3aqu - Revision history

OCA at 15:52, 4 October 2023

OCA — Wed, 04 Oct 2023 15:52:55 GMT

←Older revision		Revision as of 15:52, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='3aqu' size='340' side='right'caption='[[3aqu]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='3aqu' size='340' side='right'caption='[[3aqu]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3aqu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AQU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3aqu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AQU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.01Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>AT4g19810 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~3702 ARATH])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aqu OCA], [https://pdbe.org/3aqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aqu RCSB], [https://www.ebi.ac.uk/pdbsum/3aqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aqu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aqu OCA], [https://pdbe.org/3aqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aqu RCSB], [https://www.ebi.ac.uk/pdbsum/3aqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aqu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CHIC_ARATH CHIC_ARATH] Can hydrolyze glycol chitin and chitin oligosaccharides (e.g. N-acetylglucosamine) (GlcNAc)4, (GlcNAc)5 and (GlcNAc)6 (PubMed:21390509, PubMed:22936594). Hydrolyzes N-acetylglucosamine oligomers producing dimers from the non-reducing end of the substrates (PubMed:21390509).<ref>PMID:21390509</ref> <ref>PMID:22936594</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath]]~~	+	[[Category: Arabidopsis thaliana]]
-	~~[[Category: Chitinase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fukamizo, T]]	+	[[Category: Fukamizo T]]
-	[[Category: Numata, T]]	+	[[Category: Numata T]]
-	[[Category: Ohnuma, T]]	+	[[Category: Ohnuma T]]
-	[[Category: Osawa, T]]	+	[[Category: Osawa T]]
-	~~[[Category: Chitin]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Stress response]]~~	+
-	~~[[Category: Tim barrel~~]]	+

OCA at 15:01, 29 December 2021

OCA — Wed, 29 Dec 2021 15:01:38 GMT

←Older revision		Revision as of 15:01, 29 December 2021
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	==Crystal structure of a class V chitinase from Arabidopsis thaliana==		==Crystal structure of a class V chitinase from Arabidopsis thaliana==
-	<StructureSection load='3aqu' size='340' side='right' caption='[[3aqu]], [[Resolution\|resolution]] 2.01Å' scene=''>	+	<StructureSection load='3aqu' size='340' side='right'caption='[[3aqu]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3aqu]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3AQU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3aqu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AQU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AT4g19810 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AT4g19810 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aqu OCA], [~~http~~://pdbe.org/3aqu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3aqu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3aqu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3aqu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aqu OCA], [https://pdbe.org/3aqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aqu RCSB], [https://www.ebi.ac.uk/pdbsum/3aqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aqu ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 24:
	[[Category: Arath]]		[[Category: Arath]]
	[[Category: Chitinase]]		[[Category: Chitinase]]
		+	[[Category: Large Structures]]
	[[Category: Fukamizo, T]]		[[Category: Fukamizo, T]]
	[[Category: Numata, T]]		[[Category: Numata, T]]

OCA at 08:53, 5 August 2016

OCA — Fri, 05 Aug 2016 08:53:50 GMT

←Older revision		Revision as of 08:53, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of a class V chitinase from Arabidopsis thaliana==		==Crystal structure of a class V chitinase from Arabidopsis thaliana==
	<StructureSection load='3aqu' size='340' side='right' caption='[[3aqu]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='3aqu' size='340' side='right' caption='[[3aqu]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Arabidopsis_thaliana Arabidopsis thaliana~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AQU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AQU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AT4g19810 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ~~Arabidopsis thaliana~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AT4g19810 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aqu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aqu RCSB], [http://www.ebi.ac.uk/pdbsum/3aqu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aqu OCA], [http://pdbe.org/3aqu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3aqu RCSB], [http://www.ebi.ac.uk/pdbsum/3aqu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3aqu ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3aqu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arabidopsis thaliana~~]]	+	[[Category: Arath]]
	[[Category: Chitinase]]		[[Category: Chitinase]]
	[[Category: Fukamizo, T]]		[[Category: Fukamizo, T]]

OCA at 21:59, 3 January 2015

OCA — Sat, 03 Jan 2015 21:59:19 GMT

←Older revision		Revision as of 21:59, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AQU FirstGlance]. <br>		<table><tr><td colspan='2'>[[3aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AQU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AT4g19810 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AT4g19810 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aqu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aqu RCSB], [http://www.ebi.ac.uk/pdbsum/3aqu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aqu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aqu RCSB], [http://www.ebi.ac.uk/pdbsum/3aqu PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis.,Ohnuma T, Numata T, Osawa T, Mizuhara M, Lampela O, Juffer AH, Skriver K, Fukamizo T Planta. 2011 Jul;234(1):123-37. Epub 2011 Mar 9. PMID:21390509<ref>PMID:21390509</ref>		A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis.,Ohnuma T, Numata T, Osawa T, Mizuhara M, Lampela O, Juffer AH, Skriver K, Fukamizo T Planta. 2011 Jul;234(1):123-37. Epub 2011 Mar 9. PMID:21390509<ref>PMID:21390509</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 22:		Line 22:
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Chitinase]]		[[Category: Chitinase]]
-	[[Category: Fukamizo, T.]]	+	[[Category: Fukamizo, T]]
-	[[Category: Numata, T.]]	+	[[Category: Numata, T]]
-	[[Category: Ohnuma, T.]]	+	[[Category: Ohnuma, T]]
-	[[Category: Osawa, T.]]	+	[[Category: Osawa, T]]
	[[Category: Chitin]]		[[Category: Chitin]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Stress response]]		[[Category: Stress response]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]

OCA at 11:08, 14 May 2014

OCA — Wed, 14 May 2014 11:08:36 GMT

←Older revision		Revision as of 11:08, 14 May 2014
Line 1:		Line 1:
-	[[~~Image:~~3aqu.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of a class V chitinase from Arabidopsis thaliana==
		+	<StructureSection load='3aqu' size='340' side='right' caption='[[3aqu]], [[Resolution\|resolution]] 2.01Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AQU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AT4g19810 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aqu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aqu RCSB], [http://www.ebi.ac.uk/pdbsum/3aqu PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Expression of a class V chitinase gene (At4g19810, AtChiC) in Arabidopsis thaliana was examined by quantitative real-time PCR and by analyzing microarray data available at Genevestigator. The gene expression was induced by the plant stress-related hormones abscisic acid (ABA) and jasmonic acid (JA) and by the stress resulting from the elicitor flagellin, NaCl, and osmosis. The recombinant AtChiC protein was produced in E. coli, purified, and characterized with respect to the structure and function. The recombinant AtChiC hydrolyzed N-acetylglucosamine oligomers producing dimers from the non-reducing end of the substrates. The crystal structure of AtChiC was determined by the molecular replacement method at 2.0 A resolution. AtChiC was found to adopt an (beta/alpha)(8) fold with a small insertion domain composed of an alpha-helix and a five-stranded beta-sheet. From docking simulation of AtChiC with pentameric substrate, the amino acid residues responsible for substrate binding were found to be well conserved when compared with those of the class V chitinase from Nicotiana tabacum (NtChiV). All of the structural and functional properties of AtChiC are quite similar to those obtained for NtChiV, and seem to be common to class V chitinases from higher plants.

-	~~<!--~~	+	A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis.,Ohnuma T, Numata T, Osawa T, Mizuhara M, Lampela O, Juffer AH, Skriver K, Fukamizo T Planta. 2011 Jul;234(1):123-37. Epub 2011 Mar 9. PMID:21390509<ref>PMID:21390509</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3aqu"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3aqu\| PDB=3aqu \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~a class V chitinase from Arabidopsis thaliana===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21390509}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21390509 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21390509}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3aqu]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQU OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021390509<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Chitinase]]		[[Category: Chitinase]]

OCA at 06:12, 27 July 2011

OCA — Wed, 27 Jul 2011 06:12:04 GMT

←Older revision		Revision as of 06:12, 27 July 2011
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-	[[Image:3aqu.~~jpg~~\|left\|200px]]	+	[[Image:3aqu.png\|left\|200px]]

	<!--		<!--

OCA at 06:47, 20 July 2011

OCA — Wed, 20 Jul 2011 06:47:12 GMT

←Older revision		Revision as of 06:47, 20 July 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3aqu.jpg\|left\|200px]]

-	The ~~entry 3aqu~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3aqu", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3aqu\| PDB=3aqu \| SCENE= }}

-	~~Authors: Numata, T., Ohnuma, T., Osawa, T., Fukamizo, T.~~	+	===Crystal structure of a class V chitinase from Arabidopsis thaliana===

-	~~Description~~: ~~Crystal structure of~~ a ~~class V chitinase~~ from Arabidopsis thaliana	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21390509}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21390509 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21390509}}
		+
		+	==About this Structure==
		+	[[3aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQU OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021390509</ref><references group="xtra"/>
		+	[[Category: Arabidopsis thaliana]]
		+	[[Category: Chitinase]]
		+	[[Category: Fukamizo, T.]]
		+	[[Category: Numata, T.]]
		+	[[Category: Ohnuma, T.]]
		+	[[Category: Osawa, T.]]
		+	[[Category: Chitin]]
		+	[[Category: Hydrolase]]
		+	[[Category: Stress response]]
		+	[[Category: Tim barrel]]

OCA: Protected "3aqu" [edit=sysop:move=sysop]

OCA — Wed, 05 Jan 2011 15:48:22 GMT

Protected "3aqu" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:48, 5 January 2011

OCA at 15:48, 5 January 2011

OCA — Wed, 05 Jan 2011 15:48:21 GMT

←Older revision		Revision as of 15:48, 5 January 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3aqu is ON HOLD	+	The entry 3aqu is ON HOLD until Paper Publication

	Authors: Numata, T., Ohnuma, T., Osawa, T., Fukamizo, T.		Authors: Numata, T., Ohnuma, T., Osawa, T., Fukamizo, T.

	Description: Crystal structure of a class V chitinase from Arabidopsis thaliana		Description: Crystal structure of a class V chitinase from Arabidopsis thaliana
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 24 13:52:07 2010''

OCA: New page: '''Unreleased structure''' The entry 3aqu is ON HOLD Authors: Numata, T., Ohnuma, T., Osawa, T., Fukamizo, T. Description: Crystal structure of a class V chitinase from Arabidopsis tha...

OCA — Wed, 24 Nov 2010 10:40:37 GMT

New page: '''Unreleased structure''' The entry 3aqu is ON HOLD Authors: Numata, T., Ohnuma, T., Osawa, T., Fukamizo, T. Description: Crystal structure of a class V chitinase from Arabidopsis tha...

New page

'''Unreleased structure'''

The entry 3aqu is ON HOLD

Authors: Numata, T., Ohnuma, T., Osawa, T., Fukamizo, T.

Description: Crystal structure of a class V chitinase from Arabidopsis thaliana

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 24 13:52:07 2010''