3axi - Revision history

OCA at 08:49, 11 October 2023

OCA — Wed, 11 Oct 2023 08:49:04 GMT

←Older revision		Revision as of 08:49, 11 October 2023
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	<StructureSection load='3axi' size='340' side='right'caption='[[3axi]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='3axi' size='340' side='right'caption='[[3axi]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3axi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AXI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3axi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AXI FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3aj7\|3aj7]], [[3a4a\|3a4a]], [[3axh\|3axh]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>IMA1, FSP2, YGR287C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~4932 ATCC 18824])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Oligo~~-~~1,6~~-~~glucosidase Oligo-1,6-glucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.10 3.2.1.10]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3axi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3axi OCA], [https://pdbe.org/3axi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3axi RCSB], [https://www.ebi.ac.uk/pdbsum/3axi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3axi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3axi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3axi OCA], [https://pdbe.org/3axi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3axi RCSB], [https://www.ebi.ac.uk/pdbsum/3axi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3axi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MALX3_YEAST MALX3_YEAST]] Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization. Preferentially hydrolyzes isomaltose, palatinose, and methyl-alpha-glucoside, with little activity towards isomaltotriose or longer oligosaccharides. Does not hydrolyze maltose.<ref>PMID:15291818</ref> <ref>PMID:20562106</ref>	+	[https://www.uniprot.org/uniprot/MALX3_YEAST MALX3_YEAST] Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization. Preferentially hydrolyzes isomaltose, palatinose, and methyl-alpha-glucoside, with little activity towards isomaltotriose or longer oligosaccharides. Does not hydrolyze maltose.<ref>PMID:15291818</ref> <ref>PMID:20562106</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Oligo-1,6-glucosidase~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Kusunoki, M]]	+	[[Category: Kusunoki M]]
-	[[Category: Miyake, H]]	+	[[Category: Miyake H]]
-	[[Category: Osaki, S]]	+	[[Category: Osaki S]]
-	[[Category: Yamamoto, K]]	+	[[Category: Yamamoto K]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 11:16, 5 January 2022

OCA — Wed, 05 Jan 2022 11:16:23 GMT

←Older revision		Revision as of 11:16, 5 January 2022
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	==Crystal structure of isomaltase in complex with maltose==		==Crystal structure of isomaltase in complex with maltose==
-	<StructureSection load='3axi' size='340' side='right' caption='[[3axi]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='3axi' size='340' side='right'caption='[[3axi]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3axi]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AXI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3AXI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3axi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AXI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aj7\|3aj7]], [[3a4a\|3a4a]], [[3axh\|3axh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3aj7\|3aj7]], [[3a4a\|3a4a]], [[3axh\|3axh]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IMA1, FSP2, YGR287C ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IMA1, FSP2, YGR287C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Oligo-1,6-glucosidase Oligo-1,6-glucosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.10 3.2.1.10] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Oligo-1,6-glucosidase Oligo-1,6-glucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.10 3.2.1.10] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3axi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3axi OCA], [~~http~~://pdbe.org/3axi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3axi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3axi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3axi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3axi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3axi OCA], [https://pdbe.org/3axi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3axi RCSB], [https://www.ebi.ac.uk/pdbsum/3axi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3axi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MALX3_YEAST MALX3_YEAST]] Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization. Preferentially hydrolyzes isomaltose, palatinose, and methyl-alpha-glucoside, with little activity towards isomaltotriose or longer oligosaccharides. Does not hydrolyze maltose.<ref>PMID:15291818</ref> <ref>PMID:20562106</ref>	+	[[https://www.uniprot.org/uniprot/MALX3_YEAST MALX3_YEAST]] Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization. Preferentially hydrolyzes isomaltose, palatinose, and methyl-alpha-glucoside, with little activity towards isomaltotriose or longer oligosaccharides. Does not hydrolyze maltose.<ref>PMID:15291818</ref> <ref>PMID:20562106</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[Alpha-glucosidase\|Alpha-glucosidase]]	+	*[[Alpha-glucosidase 3D structures\|Alpha-glucosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Oligo-1,6-glucosidase]]		[[Category: Oligo-1,6-glucosidase]]
	[[Category: Kusunoki, M]]		[[Category: Kusunoki, M]]

OCA at 07:40, 5 August 2016

OCA — Fri, 05 Aug 2016 07:40:45 GMT

←Older revision		Revision as of 07:40, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of isomaltase in complex with maltose==		==Crystal structure of isomaltase in complex with maltose==
	<StructureSection load='3axi' size='340' side='right' caption='[[3axi]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='3axi' size='340' side='right' caption='[[3axi]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3axi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AXI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AXI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3axi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AXI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AXI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aj7\|3aj7]], [[3a4a\|3a4a]], [[3axh\|3axh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aj7\|3aj7]], [[3a4a\|3a4a]], [[3axh\|3axh]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IMA1, FSP2, YGR287C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IMA1, FSP2, YGR287C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oligo-1,6-glucosidase Oligo-1,6-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.10 3.2.1.10] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oligo-1,6-glucosidase Oligo-1,6-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.10 3.2.1.10] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3axi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3axi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3axi RCSB], [http://www.ebi.ac.uk/pdbsum/3axi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3axi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3axi OCA], [http://pdbe.org/3axi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3axi RCSB], [http://www.ebi.ac.uk/pdbsum/3axi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3axi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3axi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 18824]]
	[[Category: Oligo-1,6-glucosidase]]		[[Category: Oligo-1,6-glucosidase]]
-	[[Category: Saccharomyces cerevisiae]]
	[[Category: Kusunoki, M]]		[[Category: Kusunoki, M]]
	[[Category: Miyake, H]]		[[Category: Miyake, H]]

OCA at 16:58, 25 December 2014

OCA — Thu, 25 Dec 2014 16:58:40 GMT

←Older revision		Revision as of 16:58, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3axi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3axi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3axi RCSB], [http://www.ebi.ac.uk/pdbsum/3axi PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3axi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3axi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3axi RCSB], [http://www.ebi.ac.uk/pdbsum/3axi PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MALX3_YEAST MALX3_YEAST]] Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization. Preferentially hydrolyzes isomaltose, palatinose, and methyl-alpha-glucoside, with little activity towards isomaltotriose or longer oligosaccharides. Does not hydrolyze maltose.<ref>PMID:15291818</ref> <ref>PMID:20562106</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 14:17, 18 December 2014

OCA — Thu, 18 Dec 2014 14:17:06 GMT

←Older revision		Revision as of 14:17, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3axi\| PDB~~=3axi \| ~~SCENE~~= }}	+	==Crystal structure of isomaltase in complex with maltose==
-	===~~Crystal~~ structure of isomaltase in complex with maltose~~===~~	+	<StructureSection load='3axi' size='340' side='right' caption='[[3axi]], [[Resolution\|resolution]] 1.40Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21925939}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3axi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AXI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AXI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aj7\|3aj7]], [[3a4a\|3a4a]], [[3axh\|3axh]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IMA1, FSP2, YGR287C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oligo-1,6-glucosidase Oligo-1,6-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.10 3.2.1.10] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3axi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3axi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3axi RCSB], [http://www.ebi.ac.uk/pdbsum/3axi PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The structures of the E277A isomaltase mutant from Saccharomyces cerevisiae in complex with isomaltose or maltose were determined at resolutions of 1.80 and 1.40A, respectively. The root mean square deviations between the corresponding main-chain atoms of free isomaltase and the E277Alpha-isomaltose complex structures and those of free isomaltase and the E277A-maltose complex structures were found to be 0.131A and 0.083A, respectively. Thus, the amino acid substitution and ligand binding do not affect the overall structure of isomaltase. In the E277A-isomaltose structure, the bound isomaltose was readily identified by electron densities in the active site pocket; however, the reducing end of maltose was not observed in the E277A-maltose structure. The superposition of maltose onto the E277A-maltose structure revealed that the reducing end of maltose cannot bind to the subsite +1 due to the steric hindrance from Val216 and Gln279. The amino acid sequence comparisons with alpha-glucosidases showed that a bulky hydrophobic amino acid residue is conserved at the position of Val216 in alpha-1,6-glucosidic linkage hydrolyzing enzymes. Similarly, a bulky amino acid residue is conserved at the position of Gln279 in alpha-1,6-glucosidic linkage-only hydrolyzing alpha-glucosidases. Ala, Gly, or Asn residues were located at the position of alpha-1,4-glucosidic linkage hydrolyzing alpha-glucosidases. Two isomaltase mutant enzymes - V216T and Q279A - hydrolyzed maltose. Thus, the amino acid residues at these positions may be largely responsible for determining the substrate specificity of alpha-glucosidases.

-	~~==Function==~~	+	Steric hindrance by 2 amino acid residues determines the substrate specificity of isomaltase from Saccharomyces cerevisiae.,Yamamoto K, Miyake H, Kusunoki M, Osaki S J Biosci Bioeng. 2011 Sep 16. PMID:21925939<ref>PMID:21925939</ref>
-	~~[[http://www.uniprot.org/uniprot/MALX3_YEAST MALX3_YEAST]] Major~~ isomaltase ~~(alpha-1,6-glucosidase) required for isomaltose utilization~~. ~~Preferentially hydrolyzes isomaltose~~, ~~palatinose~~, ~~and methyl-alpha-glucoside~~, ~~with little activity towards isomaltotriose or longer oligosaccharides~~. ~~Does not hydrolyze maltose~~.~~<ref>~~PMID:~~15291818</ref>~~ <ref>PMID:~~20562106~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3axi]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3AXI OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:021925939</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Alpha-glucosidase\|Alpha-glucosidase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Oligo-1,6-glucosidase]]		[[Category: Oligo-1,6-glucosidase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Kusunoki, M.]]	+	[[Category: Kusunoki, M]]
-	[[Category: Miyake, H.]]	+	[[Category: Miyake, H]]
-	[[Category: Osaki, S.]]	+	[[Category: Osaki, S]]
-	[[Category: Yamamoto, K.]]	+	[[Category: Yamamoto, K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 09:32, 19 June 2013

OCA — Wed, 19 Jun 2013 09:32:23 GMT

←Older revision		Revision as of 09:32, 19 June 2013
Line 1:		Line 1:
-	[[Image:3axi.jpg\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3axi", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3axi\| PDB=3axi \| SCENE= }}		{{STRUCTURE_3axi\| PDB=3axi \| SCENE= }}
-
	===Crystal structure of isomaltase in complex with maltose===		===Crystal structure of isomaltase in complex with maltose===
		+	{{ABSTRACT_PUBMED_21925939}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/MALX3_YEAST MALX3_YEAST]] Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization. Preferentially hydrolyzes isomaltose, palatinose, and methyl-alpha-glucoside, with little activity towards isomaltotriose or longer oligosaccharides. Does not hydrolyze maltose.<ref>PMID:15291818</ref> <ref>PMID:20562106</ref>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21925939}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov)~~, ~~where 21925939 is the PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_21925939}}~~	+

	==About this Structure==		==About this Structure==
Line 22:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:021925939</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021925939</ref><references group="xtra"/><references/>
	[[Category: Oligo-1,6-glucosidase]]		[[Category: Oligo-1,6-glucosidase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]

OCA at 06:35, 5 October 2011

OCA — Wed, 05 Oct 2011 06:35:27 GMT

←Older revision		Revision as of 06:35, 5 October 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3axi.jpg\|left\|200px]]

-	The ~~entry 3axi~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3axi", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3axi\| PDB=3axi \| SCENE= }}

-	~~Authors: Yamamoto, K., Miyake, H., Kusunoki, M., Osaki, S.~~	+	===Crystal structure of isomaltase in complex with maltose===

-	~~Description~~: ~~Crystal~~ structure ~~of isomaltase in complex~~ with ~~maltose~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21925939}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21925939 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21925939}}
		+
		+	==About this Structure==
		+	[[3axi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AXI OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021925939</ref><references group="xtra"/>
		+	[[Category: Oligo-1,6-glucosidase]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Kusunoki, M.]]
		+	[[Category: Miyake, H.]]
		+	[[Category: Osaki, S.]]
		+	[[Category: Yamamoto, K.]]
		+	[[Category: Hydrolase]]

OCA at 10:11, 21 April 2011

OCA — Thu, 21 Apr 2011 10:11:56 GMT

←Older revision		Revision as of 10:11, 21 April 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3axi is ON HOLD	+	The entry 3axi is ON HOLD until Paper Publication

	Authors: Yamamoto, K., Miyake, H., Kusunoki, M., Osaki, S.		Authors: Yamamoto, K., Miyake, H., Kusunoki, M., Osaki, S.

-	Description: Crystal structure of ~~isomaltose~~ in complex with maltose	+	Description: Crystal structure of isomaltase in complex with maltose

OCA: Protected "3axi" [edit=sysop:move=sysop]

OCA — Wed, 13 Apr 2011 04:49:33 GMT

Protected "3axi" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:49, 13 April 2011

OCA: New page: '''Unreleased structure''' The entry 3axi is ON HOLD Authors: Yamamoto, K., Miyake, H., Kusunoki, M., Osaki, S. Description: Crystal structure of isomaltose in complex with maltose

OCA — Wed, 13 Apr 2011 04:49:32 GMT

New page: '''Unreleased structure''' The entry 3axi is ON HOLD Authors: Yamamoto, K., Miyake, H., Kusunoki, M., Osaki, S. Description: Crystal structure of isomaltose in complex with maltose

New page

'''Unreleased structure'''

The entry 3axi is ON HOLD

Authors: Yamamoto, K., Miyake, H., Kusunoki, M., Osaki, S.

Description: Crystal structure of isomaltose in complex with maltose