3b2e - Revision history

OCA at 08:52, 11 October 2023

OCA — Wed, 11 Oct 2023 08:52:28 GMT

←Older revision		Revision as of 08:52, 11 October 2023
Line 3:		Line 3:
	<StructureSection load='3b2e' size='340' side='right'caption='[[3b2e]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='3b2e' size='340' side='right'caption='[[3b2e]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3b2e]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~] and [https://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B2E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3b2e]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B2E FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADP:ADENOSINE~~-~~5-DIPHOSPHATE'>ADP</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3vlc\|3vlc]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>GET1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=559292 Baker's yeast])</td></tr>~~	+
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Arsenite~~-~~transporting_ATPase Arsenite~~-~~transporting ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b2e OCA], [https://pdbe.org/3b2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b2e RCSB], [https://www.ebi.ac.uk/pdbsum/3b2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b2e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b2e OCA], [https://pdbe.org/3b2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b2e RCSB], [https://www.ebi.ac.uk/pdbsum/3b2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b2e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~GET1_YEAST GET1_YEAST~~]~~] Required~~ for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. ~~Together with GET2, acts as a membrane receptor for soluble GET3, which recognizes~~ and selectively binds the transmembrane domain of TA proteins in the cytosol. ~~The GET~~ complex ~~cooperates~~ with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in ~~mitochondrial distribution~~ and ~~morphology~~.<ref>PMID:~~11907266~~</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:~~21835666~~</ref> <ref>PMID:21719644</ref>	+	[https://www.uniprot.org/uniprot/GET3_YEAST GET3_YEAST] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.<ref>PMID:12680698</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21866104</ref> <ref>PMID:21719644</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Arsenite-transporting ATPase]]
-	[[Category: Atcc 18824]]
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Fukai, S~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Kubota, K~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Yamagata, A~~]]	+	[[Category: Fukai S]]
-	[[Category: ~~Adp binding~~]]	+	[[Category: Kubota K]]
-	[[Category: ~~Coild-coil]]~~	+	[[Category: Yamagata A]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-transport protein complex]]~~	+
-	~~[[Category: Protein-protein interaction]]~~	+
-	~~[[Category: Receptor complex]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 11:21, 5 January 2022

OCA — Wed, 05 Jan 2022 11:21:21 GMT

←Older revision		Revision as of 11:21, 5 January 2022
Line 1:		Line 1:

	==Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain==		==Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain==
-	<StructureSection load='3b2e' size='340' side='right' caption='[[3b2e]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='3b2e' size='340' side='right'caption='[[3b2e]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3b2e]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [~~http~~://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3B2E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3b2e]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B2E FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vlc\|3vlc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3vlc\|3vlc]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GET1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GET1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3b2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b2e OCA], [~~http~~://pdbe.org/3b2e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3b2e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3b2e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3b2e ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b2e OCA], [https://pdbe.org/3b2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b2e RCSB], [https://www.ebi.ac.uk/pdbsum/3b2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b2e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GET1_YEAST GET1_YEAST]] Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET2, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in mitochondrial distribution and morphology.<ref>PMID:11907266</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21835666</ref> <ref>PMID:21719644</ref>	+	[[https://www.uniprot.org/uniprot/GET1_YEAST GET1_YEAST]] Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET2, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in mitochondrial distribution and morphology.<ref>PMID:11907266</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21835666</ref> <ref>PMID:21719644</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[ATPase\|ATPase]]	+	*[[ATPase 3D structures\|ATPase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 31:
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Fukai, S]]		[[Category: Fukai, S]]
	[[Category: Kubota, K]]		[[Category: Kubota, K]]

OCA at 10:02, 4 August 2016

OCA — Thu, 04 Aug 2016 10:02:27 GMT

←Older revision		Revision as of 10:02, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain==		==Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain==
	<StructureSection load='3b2e' size='340' side='right' caption='[[3b2e]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='3b2e' size='340' side='right' caption='[[3b2e]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3b2e]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~] and [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B2E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3b2e]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B2E FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vlc\|3vlc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vlc\|3vlc]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GET1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 ~~Saccharomyces cerevisiae S288c~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GET1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b2e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b2e RCSB], [http://www.ebi.ac.uk/pdbsum/3b2e PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b2e OCA], [http://pdbe.org/3b2e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b2e RCSB], [http://www.ebi.ac.uk/pdbsum/3b2e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3b2e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3b2e" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Arsenite-transporting ATPase]]		[[Category: Arsenite-transporting ATPase]]
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
-	[[Category: ~~Saccharomyces cerevisiae s288c~~]]	+	[[Category: Baker's yeast]]
	[[Category: Fukai, S]]		[[Category: Fukai, S]]
	[[Category: Kubota, K]]		[[Category: Kubota, K]]

OCA at 08:40, 24 December 2014

OCA — Wed, 24 Dec 2014 08:40:35 GMT

←Older revision		Revision as of 08:40, 24 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b2e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b2e RCSB], [http://www.ebi.ac.uk/pdbsum/3b2e PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b2e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b2e RCSB], [http://www.ebi.ac.uk/pdbsum/3b2e PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GET1_YEAST GET1_YEAST]] Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET2, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in mitochondrial distribution and morphology.<ref>PMID:11907266</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21835666</ref> <ref>PMID:21719644</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 13:36, 18 December 2014

OCA — Thu, 18 Dec 2014 13:36:01 GMT

←Older revision		Revision as of 13:36, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3b2e\| PDB=3b2e \| SCENE= }}~~	+	==Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain==
-	===Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain===	+	<StructureSection load='3b2e' size='340' side='right' caption='[[3b2e]], [[Resolution\|resolution]] 3.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_22684149}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3b2e]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B2E FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vlc\|3vlc]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GET1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b2e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b2e RCSB], [http://www.ebi.ac.uk/pdbsum/3b2e PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tail-anchored (TA) proteins are integral membrane proteins that possess a single transmembrane domain near their carboxy terminus. TA proteins play critical roles in many important cellular processes such as membrane trafficking, protein translocation, and apoptosis. The GET complex mediates posttranslational insertion of newly synthesized TA proteins to the endoplasmic reticulum membrane. The GET complex is composed of the homodimeric Get3 ATPase and its heterooligomeric receptor, Get1/2. During insertion, the Get3 dimer shuttles between open and closed conformational states, coupled with ATP hydrolysis and the binding/release of TA proteins. We report crystal structures of ADP-bound Get3 in complex with the cytoplasmic domain of Get1 (Get1CD) in open and semi-open conformations at 3.0- and 4.5-A resolutions, respectively. Our structures and biochemical data suggest that Get1 uses two interfaces to stabilize the open dimer conformation of Get3. We propose that one interface is sufficient for binding of Get1 by Get3, while the second interface stabilizes the open dimer conformation of Get3.

-	~~==Function==~~	+	Get1 Stabilizes an Open Dimer Conformation of Get3 ATPase by Binding Two Distinct Interfaces.,Kubota K, Yamagata A, Sato Y, Goto-Ito S, Fukai S J Mol Biol. 2012 Jun 7. PMID:22684149<ref>PMID:22684149</ref>
-	~~[[http://www.uniprot.org/uniprot/GET1_YEAST GET1_YEAST]] Required for the post-translational delivery~~ of ~~tail-anchored (TA) proteins to the endoplasmic reticulum~~. ~~Together with GET2~~, ~~acts as a membrane receptor for soluble GET3~~, ~~which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP~~-~~dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER~~. ~~Involved in mitochondrial distribution and morphology~~.~~<ref>~~PMID:~~11907266</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21835666</ref>~~ <ref>PMID:~~21719644~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3b2e]] is~~ a ~~8 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en~~.~~wikipedia.org~~/~~wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2E OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:022684149</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[ATPase\|ATPase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Arsenite-transporting ATPase]]		[[Category: Arsenite-transporting ATPase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Saccharomyces cerevisiae s288c]]		[[Category: Saccharomyces cerevisiae s288c]]
-	[[Category: Fukai, S.]]	+	[[Category: Fukai, S]]
-	[[Category: Kubota, K.]]	+	[[Category: Kubota, K]]
-	[[Category: Yamagata, A.]]	+	[[Category: Yamagata, A]]
	[[Category: Adp binding]]		[[Category: Adp binding]]
	[[Category: Coild-coil]]		[[Category: Coild-coil]]

OCA at 07:32, 20 June 2013

OCA — Thu, 20 Jun 2013 07:32:55 GMT

←Older revision		Revision as of 07:32, 20 June 2013
Line 1:		Line 1:
-	[[Image:3b2e.jpg\|left\|200px]]
-
	{{STRUCTURE_3b2e\| PDB=3b2e \| SCENE= }}		{{STRUCTURE_3b2e\| PDB=3b2e \| SCENE= }}
-
	===Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain===		===Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain===
		+	{{ABSTRACT_PUBMED_22684149}}

-	~~{{ABSTRACT_PUBMED_22684149}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/GET1_YEAST GET1_YEAST]] Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET2, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in mitochondrial distribution and morphology.<ref>PMID:11907266</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21835666</ref> <ref>PMID:21719644</ref>

	==About this Structure==		==About this Structure==
-	[[3b2e]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2E OCA].	+	[[3b2e]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2E OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:022684149</ref><references group="xtra"/>	+	<ref group="xtra">PMID:022684149</ref><references group="xtra"/><references/>
	[[Category: Arsenite-transporting ATPase]]		[[Category: Arsenite-transporting ATPase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Saccharomyces cerevisiae s288c]]
	[[Category: Fukai, S.]]		[[Category: Fukai, S.]]
	[[Category: Kubota, K.]]		[[Category: Kubota, K.]]

OCA at 05:45, 27 June 2012

OCA — Wed, 27 Jun 2012 05:45:10 GMT

←Older revision		Revision as of 05:45, 27 June 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3b2e.jpg\|left\|200px]]

-	~~The entry~~ 3b2e ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3b2e\| PDB=3b2e \| SCENE= }}

-	~~Authors: Kubota, K., Yamagata, A., Fukai,~~ S.	+	===Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain===

-	~~Description: Crystal~~ structure ~~of S~~. cerevisiae ~~Get3 in the open conformation in~~ complex ~~with Get1 cytosolic domain~~	+	{{ABSTRACT_PUBMED_22684149}}
		+
		+	==About this Structure==
		+	[[3b2e]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2E OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022684149</ref><references group="xtra"/>
		+	[[Category: Arsenite-transporting ATPase]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Fukai, S.]]
		+	[[Category: Kubota, K.]]
		+	[[Category: Yamagata, A.]]
		+	[[Category: Adp binding]]
		+	[[Category: Coild-coil]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase-transport protein complex]]
		+	[[Category: Protein-protein interaction]]
		+	[[Category: Receptor complex]]
		+	[[Category: Transport protein]]

OCA at 05:53, 14 September 2011

OCA — Wed, 14 Sep 2011 05:53:52 GMT

←Older revision		Revision as of 05:53, 14 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3b2e is ON HOLD	+	The entry 3b2e is ON HOLD until Paper Publication

	Authors: Kubota, K., Yamagata, A., Fukai, S.		Authors: Kubota, K., Yamagata, A., Fukai, S.

	Description: Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain		Description: Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain

OCA at 06:12, 31 August 2011

OCA — Wed, 31 Aug 2011 06:12:26 GMT

←Older revision		Revision as of 06:12, 31 August 2011
Line 3:		Line 3:
	The entry 3b2e is ON HOLD		The entry 3b2e is ON HOLD

-	Authors: Kubota, K, Yamagata, A, Fukai, S	+	Authors: Kubota, K., Yamagata, A., Fukai, S.

	Description: Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain		Description: Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain

OCA: Protected "3b2e" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2011 05:22:06 GMT

Protected "3b2e" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:22, 10 August 2011