3b5d - Revision history

OCA at 09:27, 21 February 2024

OCA — Wed, 21 Feb 2024 09:27:35 GMT

←Older revision		Revision as of 09:27, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3b5d' size='340' side='right'caption='[[3b5d]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='3b5d' size='340' side='right'caption='[[3b5d]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3b5d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B5D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3b5d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B5D FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.8Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~emrE, eb, mvrC ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5d OCA], [https://pdbe.org/3b5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b5d RCSB], [https://www.ebi.ac.uk/pdbsum/3b5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b5d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5d OCA], [https://pdbe.org/3b5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b5d RCSB], [https://www.ebi.ac.uk/pdbsum/3b5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b5d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI]] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>	+	[https://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b5d ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b5d ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	EmrE, a multidrug transporter from Escherichia coli, functions as a homodimer of a small four-transmembrane protein. The membrane insertion topology of the two monomers is controversial. Although the EmrE protein was reported to have a unique orientation in the membrane, models based on electron microscopy and now defunct x-ray structures, as well as recent biochemical studies, posit an antiparallel dimer. We have now reanalyzed our x-ray data on EmrE. The corrected structures in complex with a transport substrate are highly similar to the electron microscopy structure. The first three transmembrane helices from each monomer surround the substrate binding chamber, whereas the fourth helices participate only in dimer formation. Selenomethionine markers clearly indicate an antiparallel orientation for the monomers, supporting a "dual topology" model.
-
-	X-ray structure of EmrE supports dual topology model.,Chen YJ, Pornillos O, Lieu S, Ma C, Chen AP, Chang G Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18999-9004. Epub 2007 Nov, 16. PMID:18024586<ref>PMID:18024586</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3b5d" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chang, G]]	+	[[Category: Chang G]]
-	[[Category: Chen~~, Y J]]~~	+	[[Category: Chen YJ]]
-	~~[[Category: Antiport]]~~	+
-	~~[[Category: Helical membrane protein]]~~	+
-	~~[[Category: Inner membrane]]~~	+
-	~~[[Category: Joint center for innovative membrane protein technology]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Multidrug resistance transporter]]~~	+
-	~~[[Category: Smr]]~~	+
-	~~[[Category: Transmembrane~~]]	+

OCA at 11:24, 5 January 2022

OCA — Wed, 05 Jan 2022 11:24:01 GMT

←Older revision		Revision as of 11:24, 5 January 2022
Line 1:		Line 1:

	==EmrE multidrug transporter in complex with TPP, C2 crystal form==		==EmrE multidrug transporter in complex with TPP, C2 crystal form==
-	<StructureSection load='3b5d' size='340' side='right' caption='[[3b5d]], [[Resolution\|resolution]] 3.80Å' scene=''>	+	<StructureSection load='3b5d' size='340' side='right'caption='[[3b5d]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3b5d]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3B5D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3b5d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B5D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, eb, mvrC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, eb, mvrC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3b5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5d OCA], [~~http~~://pdbe.org/3b5d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3b5d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3b5d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3b5d ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5d OCA], [https://pdbe.org/3b5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b5d RCSB], [https://www.ebi.ac.uk/pdbsum/3b5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b5d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI]] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>	+	[[https://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI]] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Chang, G]]		[[Category: Chang, G]]
	[[Category: Chen, Y J]]		[[Category: Chen, Y J]]

OCA at 07:48, 31 October 2018

OCA — Wed, 31 Oct 2018 07:48:18 GMT

←Older revision		Revision as of 07:48, 31 October 2018
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/3b5d_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/3b5d_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 22:40, 5 August 2016

OCA — Fri, 05 Aug 2016 22:40:02 GMT

←Older revision		Revision as of 22:40, 5 August 2016
Line 1:		Line 1:
		+
	==EmrE multidrug transporter in complex with TPP, C2 crystal form==		==EmrE multidrug transporter in complex with TPP, C2 crystal form==
	<StructureSection load='3b5d' size='340' side='right' caption='[[3b5d]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='3b5d' size='340' side='right' caption='[[3b5d]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3b5d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B5D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3b5d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B5D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, eb, mvrC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ~~Escherichia coli K-12~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, eb, mvrC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b5d RCSB], [http://www.ebi.ac.uk/pdbsum/3b5d PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5d OCA], [http://pdbe.org/3b5d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b5d RCSB], [http://www.ebi.ac.uk/pdbsum/3b5d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3b5d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b5d ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3b5d" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli k-12~~]]	+	[[Category: Ecoli]]
	[[Category: Chang, G]]		[[Category: Chang, G]]
	[[Category: Chen, Y J]]		[[Category: Chen, Y J]]

OCA at 22:32, 25 December 2014

OCA — Thu, 25 Dec 2014 22:32:20 GMT

←Older revision		Revision as of 22:32, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b5d RCSB], [http://www.ebi.ac.uk/pdbsum/3b5d PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b5d RCSB], [http://www.ebi.ac.uk/pdbsum/3b5d PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI]] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
-	[[Category: Chang, G.]]	+	[[Category: Chang, G]]
-	[[Category: Chen, Y J.]]	+	[[Category: Chen, Y J]]
	[[Category: Antiport]]		[[Category: Antiport]]
	[[Category: Helical membrane protein]]		[[Category: Helical membrane protein]]

OCA at 06:32, 10 October 2014

OCA — Fri, 10 Oct 2014 06:32:09 GMT

←Older revision		Revision as of 06:32, 10 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3b5d\| PDB=3b5d \| SCENE= }}~~	+	==EmrE multidrug transporter in complex with TPP, C2 crystal form==
-	===EmrE multidrug transporter in complex with TPP, C2 crystal form===	+	<StructureSection load='3b5d' size='340' side='right' caption='[[3b5d]], [[Resolution\|resolution]] 3.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_18024586}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3b5d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B5D FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, eb, mvrC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b5d RCSB], [http://www.ebi.ac.uk/pdbsum/3b5d PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/3b5d_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	EmrE, a multidrug transporter from Escherichia coli, functions as a homodimer of a small four-transmembrane protein. The membrane insertion topology of the two monomers is controversial. Although the EmrE protein was reported to have a unique orientation in the membrane, models based on electron microscopy and now defunct x-ray structures, as well as recent biochemical studies, posit an antiparallel dimer. We have now reanalyzed our x-ray data on EmrE. The corrected structures in complex with a transport substrate are highly similar to the electron microscopy structure. The first three transmembrane helices from each monomer surround the substrate binding chamber, whereas the fourth helices participate only in dimer formation. Selenomethionine markers clearly indicate an antiparallel orientation for the monomers, supporting a "dual topology" model.

-	~~==Function==~~	+	X-ray structure of EmrE supports dual topology model.,Chen YJ, Pornillos O, Lieu S, Ma C, Chen AP, Chang G Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18999-9004. Epub 2007 Nov, 16. PMID:18024586<ref>PMID:18024586</ref>
-	~~[[http://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI]] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane~~ of ~~E.coli~~., ~~thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen~~, ~~ethidium bromide and acriflavine~~. ~~Is also able to transport tetraphenylphosphonium~~ (~~TPP(+~~)~~) and benzalkonium~~.~~<ref>~~PMID:~~7896833</ref> <ref>PMID:10681497</ref>~~ <ref>PMID:~~15371426~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3b5d]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:018024586</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
	[[Category: Chang, G.]]		[[Category: Chang, G.]]

OCA at 07:48, 30 June 2013

OCA — Sun, 30 Jun 2013 07:48:50 GMT

←Older revision		Revision as of 07:48, 30 June 2013
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-	[[Image:3b5d.png\|left\|200px]]
-
	{{STRUCTURE_3b5d\| PDB=3b5d \| SCENE= }}		{{STRUCTURE_3b5d\| PDB=3b5d \| SCENE= }}
-
	===EmrE multidrug transporter in complex with TPP, C2 crystal form===		===EmrE multidrug transporter in complex with TPP, C2 crystal form===
		+	{{ABSTRACT_PUBMED_18024586}}

-	~~{{ABSTRACT_PUBMED_18024586}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI]] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>

	==About this Structure==		==About this Structure==
Line 11:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:018024586</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018024586</ref><references group="xtra"/><references/>
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
	[[Category: Chang, G.]]		[[Category: Chang, G.]]

OCA: Protected "3b5d" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 22:53:12 GMT

Protected "3b5d" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:53, 7 January 2013

OCA at 22:53, 7 January 2013

OCA — Mon, 07 Jan 2013 22:53:07 GMT

←Older revision		Revision as of 22:53, 7 January 2013
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-	{{Seed}}
	[[Image:3b5d.png\|left\|200px]]		[[Image:3b5d.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3b5d", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3b5d\| PDB=3b5d \| SCENE= }}		{{STRUCTURE_3b5d\| PDB=3b5d \| SCENE= }}

	===EmrE multidrug transporter in complex with TPP, C2 crystal form===		===EmrE multidrug transporter in complex with TPP, C2 crystal form===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18024586}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18024586 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18024586}}		{{ABSTRACT_PUBMED_18024586}}

	==About this Structure==		==About this Structure==
-	~~3B5D~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Escherichia_coli~~ Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA].	+	[[3b5d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18024586~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018024586</ref><references group="xtra"/>
-	[[Category: Escherichia coli]]	+	[[Category: Escherichia coli k-12]]
	[[Category: Chang, G.]]		[[Category: Chang, G.]]
	[[Category: Chen, Y J.]]		[[Category: Chen, Y J.]]
Line 31:		Line 19:
	[[Category: Inner membrane]]		[[Category: Inner membrane]]
	[[Category: Joint center for innovative membrane protein technology]]		[[Category: Joint center for innovative membrane protein technology]]
		+	[[Category: Membrane protein]]
	[[Category: Multidrug resistance transporter]]		[[Category: Multidrug resistance transporter]]
	[[Category: Smr]]		[[Category: Smr]]
	[[Category: Transmembrane]]		[[Category: Transmembrane]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:48:58 2009''

OCA at 07:48, 18 February 2009

OCA — Wed, 18 Feb 2009 07:48:58 GMT

←Older revision		Revision as of 07:48, 18 February 2009
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	==About this Structure==		==About this Structure==
-	3B5D is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA].	+	3B5D is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA].

	==Reference==		==Reference==
-	~~X-ray structure of EmrE supports dual topology model., Chen YJ, Pornillos O, Lieu S, Ma C, Chen AP, Chang G, Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18999-9004. Epub 2007 Nov, 16.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18024586 18024586]~~	+	<ref group="xtra">PMID:18024586</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Chang, G.]]		[[Category: Chang, G.]]
	[[Category: Chen, Y J.]]		[[Category: Chen, Y J.]]
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	[[Category: Transmembrane]]		[[Category: Transmembrane]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 16~~:08:~~54 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:48:58 2009''