3bbv - Revision history

OCA at 09:28, 21 February 2024

OCA — Wed, 21 Feb 2024 09:28:22 GMT

←Older revision		Revision as of 09:28, 21 February 2024
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [https://pdbe.org/3bbv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [https://www.ebi.ac.uk/pdbsum/3bbv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bbv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [https://pdbe.org/3bbv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [https://www.ebi.ac.uk/pdbsum/3bbv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bbv ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	When heat shock prematurely dissociates a translating bacterial ribosome, its 50S subunit is prevented from reinitiating protein synthesis by tRNA covalently linked to the unfinished protein chain that remains threaded through the exit tunnel. Hsp15, a highly upregulated bacterial heat shock protein, reactivates such dead-end complexes. Here, we show with cryo-electron microscopy reconstructions and functional assays that Hsp15 translocates the tRNA moiety from the A site to the P site of stalled 50S subunits. By stabilizing the tRNA in the P site, Hsp15 indirectly frees up the A site, allowing a release factor to land there and cleave off the tRNA. Such a release factor must be stop codon independent, suggesting a possible role for a poorly characterized class of putative release factors that are upregulated by cellular stress, lack a codon recognition domain and are conserved in eukaryotes.
-
-	Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15.,Jiang L, Schaffitzel C, Bingel-Erlenmeyer R, Ban N, Korber P, Koning RI, de Geus DC, Plaisier JR, Abrahams JP J Mol Biol. 2009 Mar 13;386(5):1357-67. Epub 2008 Nov 5. PMID:19013177<ref>PMID:19013177</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3bbv" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</SX>		</SX>

OCA at 18:19, 18 October 2023

OCA — Wed, 18 Oct 2023 18:19:47 GMT

←Older revision		Revision as of 18:19, 18 October 2023
Line 3:		Line 3:
	<SX load='3bbv' size='340' side='right' viewer='molstar' caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>		<SX load='3bbv' size='340' side='right' viewer='molstar' caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BBV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BBV FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 10Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ow8\|2ow8]], [[3bbu\|3bbu]], [[3bbx\|3bbx]]</div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [https://pdbe.org/3bbv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [https://www.ebi.ac.uk/pdbsum/3bbv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bbv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [https://pdbe.org/3bbv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [https://www.ebi.ac.uk/pdbsum/3bbv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bbv ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 22:
	</SX>		</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Abrahams, J P~~]]	+	[[Category: Thermus thermophilus]]
-	[[Category: ~~Jiang, L~~]]	+	[[Category: Abrahams JP]]
-	[[Category: ~~Phe]]~~	+	[[Category: Jiang L]]
-	~~[[Category: Rescue stalled ribosome]]~~	+
-	~~[[Category: Ribosome]]~~	+
-	~~[[Category: Trna~~]]	+

OCA at 10:39, 12 January 2022

OCA — Wed, 12 Jan 2022 10:39:53 GMT

←Older revision		Revision as of 10:39, 12 January 2022
Line 3:		Line 3:
	<SX load='3bbv' size='340' side='right' viewer='molstar' caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>		<SX load='3bbv' size='340' side='right' viewer='molstar' caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3BBV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BBV FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ow8\|2ow8]], [[3bbu\|3bbu]], [[3bbx\|3bbx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ow8\|2ow8]], [[3bbu\|3bbu]], [[3bbx\|3bbx]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [~~http~~://pdbe.org/3bbv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3bbv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3bbv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [https://pdbe.org/3bbv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [https://www.ebi.ac.uk/pdbsum/3bbv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bbv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:13, 10 April 2020

OCA — Fri, 10 Apr 2020 17:13:07 GMT

←Older revision		Revision as of 17:13, 10 April 2020
Line 3:		Line 3:
	<SX load='3bbv' size='340' side='right' viewer='molstar' caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>		<SX load='3bbv' size='340' side='right' viewer='molstar' caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3BBV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3BBV FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ow8\|2ow8]], [[3bbu\|3bbu]], [[3bbx\|3bbx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ow8\|2ow8]], [[3bbu\|3bbu]], [[3bbx\|3bbx]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [http://pdbe.org/3bbv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [http://www.ebi.ac.uk/pdbsum/3bbv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bbv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [http://pdbe.org/3bbv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [http://www.ebi.ac.uk/pdbsum/3bbv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bbv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:04, 6 March 2020

OCA — Fri, 06 Mar 2020 17:04:37 GMT

←Older revision		Revision as of 17:04, 6 March 2020
Line 1:		Line 1:

	==The tRNA(phe) fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex==		==The tRNA(phe) fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex==
-	<~~StructureSection~~ load='3bbv' size='340' side='right'caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>	+	<SX load='3bbv' size='340' side='right' viewer='molstar' caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BBV FirstGlance]. <br>		<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BBV FirstGlance]. <br>
Line 20:		Line 20:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Abrahams, J P]]		[[Category: Abrahams, J P]]

OCA at 06:12, 3 July 2019

OCA — Wed, 03 Jul 2019 06:12:59 GMT

←Older revision		Revision as of 06:12, 3 July 2019
Line 1:		Line 1:

	==The tRNA(phe) fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex==		==The tRNA(phe) fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex==
-	<StructureSection load='3bbv' size='340' side='right' caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>	+	<StructureSection load='3bbv' size='340' side='right'caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BBV FirstGlance]. <br>		<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BBV FirstGlance]. <br>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Abrahams, J P]]		[[Category: Abrahams, J P]]
	[[Category: Jiang, L]]		[[Category: Jiang, L]]

OCA at 07:46, 11 August 2016

OCA — Thu, 11 Aug 2016 07:46:14 GMT

←Older revision		Revision as of 07:46, 11 August 2016
Line 1:		Line 1:
		+
	==The tRNA(phe) fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex==		==The tRNA(phe) fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex==
	<StructureSection load='3bbv' size='340' side='right' caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>		<StructureSection load='3bbv' size='340' side='right' caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BBV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BBV FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ow8\|2ow8]], [[3bbu\|3bbu]], [[3bbx\|3bbx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ow8\|2ow8]], [[3bbu\|3bbu]], [[3bbx\|3bbx]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [http://www.ebi.ac.uk/pdbsum/3bbv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [http://pdbe.org/3bbv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [http://www.ebi.ac.uk/pdbsum/3bbv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bbv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3bbv" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Thermus thermophilus]]
	[[Category: Abrahams, J P]]		[[Category: Abrahams, J P]]
	[[Category: Jiang, L]]		[[Category: Jiang, L]]

OCA at 04:39, 24 November 2014

OCA — Mon, 24 Nov 2014 04:39:01 GMT

←Older revision		Revision as of 04:39, 24 November 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BBV FirstGlance]. <br>		<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BBV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ow8\|2ow8]], [[3bbu\|3bbu]], [[3bbx\|3bbx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ow8\|2ow8]], [[3bbu\|3bbu]], [[3bbx\|3bbx]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [http://www.ebi.ac.uk/pdbsum/3bbv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [http://www.ebi.ac.uk/pdbsum/3bbv PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15.,Jiang L, Schaffitzel C, Bingel-Erlenmeyer R, Ban N, Korber P, Koning RI, de Geus DC, Plaisier JR, Abrahams JP J Mol Biol. 2009 Mar 13;386(5):1357-67. Epub 2008 Nov 5. PMID:19013177<ref>PMID:19013177</ref>		Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15.,Jiang L, Schaffitzel C, Bingel-Erlenmeyer R, Ban N, Korber P, Koning RI, de Geus DC, Plaisier JR, Abrahams JP J Mol Biol. 2009 Mar 13;386(5):1357-67. Epub 2008 Nov 5. PMID:19013177<ref>PMID:19013177</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
-	[[Category: Abrahams, J P.]]	+	[[Category: Abrahams, J P]]
-	[[Category: Jiang, L.]]	+	[[Category: Jiang, L]]
	[[Category: Phe]]		[[Category: Phe]]
	[[Category: Rescue stalled ribosome]]		[[Category: Rescue stalled ribosome]]
	[[Category: Ribosome]]		[[Category: Ribosome]]
	[[Category: Trna]]		[[Category: Trna]]

OCA: Protected "3bbv" [edit=sysop:move=sysop]

OCA — Wed, 28 May 2014 10:26:40 GMT

Protected "3bbv" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:26, 28 May 2014

OCA at 10:26, 28 May 2014

OCA — Wed, 28 May 2014 10:26:36 GMT

←Older revision		Revision as of 10:26, 28 May 2014
Line 1:		Line 1:
-	~~{{Seed}}~~	+	==The tRNA(phe) fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex==
-	[[~~Image:~~3bbv.~~png~~\|~~left~~\|~~200px~~]]	+	<StructureSection load='3bbv' size='340' side='right' caption='[[3bbv]], [[Resolution\|resolution]] 10.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3bbv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BBV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ow8\|2ow8]], [[3bbu\|3bbu]], [[3bbx\|3bbx]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bbv RCSB], [http://www.ebi.ac.uk/pdbsum/3bbv PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	When heat shock prematurely dissociates a translating bacterial ribosome, its 50S subunit is prevented from reinitiating protein synthesis by tRNA covalently linked to the unfinished protein chain that remains threaded through the exit tunnel. Hsp15, a highly upregulated bacterial heat shock protein, reactivates such dead-end complexes. Here, we show with cryo-electron microscopy reconstructions and functional assays that Hsp15 translocates the tRNA moiety from the A site to the P site of stalled 50S subunits. By stabilizing the tRNA in the P site, Hsp15 indirectly frees up the A site, allowing a release factor to land there and cleave off the tRNA. Such a release factor must be stop codon independent, suggesting a possible role for a poorly characterized class of putative release factors that are upregulated by cellular stress, lack a codon recognition domain and are conserved in eukaryotes.

-	<!--	+	Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15.,Jiang L, Schaffitzel C, Bingel-Erlenmeyer R, Ban N, Korber P, Koning RI, de Geus DC, Plaisier JR, Abrahams JP J Mol Biol. 2009 Mar 13;386(5):1357-67. Epub 2008 Nov 5. PMID:19013177<ref>PMID:19013177</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3bbv"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3bbv\| PDB=3bbv \| SCENE= }}~~	+

-	~~===The tRNA(phe) fitted into the low resolution Cryo-EM map~~ of the ~~50S~~.~~nc-tRNA~~.~~Hsp15 complex===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	<~~!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_19013177}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http:~~/~~/www.pubmed.gov), where 19013177 is the PubMed ID number.~~	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_19013177}}~~	+
-		+
-	==~~About this Structure~~==	+
-	~~3BBV is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBV OCA].~~	+
-		+
-	~~==Reference==~~	+
-	<~~ref group="xtra">PMID:19013177</ref><references group="xtra"~~/>	+
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Abrahams, J P.]]		[[Category: Abrahams, J P.]]
Line 31:		Line 26:
	[[Category: Ribosome]]		[[Category: Ribosome]]
	[[Category: Trna]]		[[Category: Trna]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 2 08:25:20 2010''