3bdz - Revision history

OCA at 14:44, 1 November 2023

OCA — Wed, 01 Nov 2023 14:44:57 GMT

←Older revision		Revision as of 14:44, 1 November 2023
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	<StructureSection load='3bdz' size='340' side='right'caption='[[3bdz]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3bdz' size='340' side='right'caption='[[3bdz]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bdz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_51113 Atcc 51113~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BDZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bdz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BDZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~, ~~<scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1t2b\|1t2b]], [[3be0\|3be0]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">cinA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 ATCC 51113])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bdz OCA], [https://pdbe.org/3bdz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bdz RCSB], [https://www.ebi.ac.uk/pdbsum/3bdz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bdz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bdz OCA], [https://pdbe.org/3bdz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bdz RCSB], [https://www.ebi.ac.uk/pdbsum/3bdz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bdz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CINA_CITBR CINA_CITBR]] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.	+	[https://www.uniprot.org/uniprot/CINA_CITBR CINA_CITBR] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 51113~~]]	+	[[Category: Citrobacter braakii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Meharenna~~, Y T~~]]	+	[[Category: Meharenna YT]]
-	[[Category: Poulos~~, T L]]~~	+	[[Category: Poulos TL]]
-	~~[[Category: Protein in the absence of substrate]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 07:26, 10 November 2021

OCA — Wed, 10 Nov 2021 07:26:39 GMT

←Older revision		Revision as of 07:26, 10 November 2021
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	==The Role of Asn 242 in P450cin==		==The Role of Asn 242 in P450cin==
-	<StructureSection load='3bdz' size='340' side='right' caption='[[3bdz]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3bdz' size='340' side='right'caption='[[3bdz]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bdz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_51113 Atcc 51113]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3BDZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bdz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_51113 Atcc 51113]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BDZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2b\|1t2b]], [[3be0\|3be0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1t2b\|1t2b]], [[3be0\|3be0]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cinA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 ATCC 51113])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cinA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 ATCC 51113])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bdz OCA], [~~http~~://pdbe.org/3bdz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3bdz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3bdz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3bdz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bdz OCA], [https://pdbe.org/3bdz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bdz RCSB], [https://www.ebi.ac.uk/pdbsum/3bdz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bdz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CINA_CITBR CINA_CITBR]] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.	+	[[https://www.uniprot.org/uniprot/CINA_CITBR CINA_CITBR]] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Cytochrome P450\|Cytochrome P450]]	+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 51113]]		[[Category: Atcc 51113]]
		+	[[Category: Large Structures]]
	[[Category: Meharenna, Y T]]		[[Category: Meharenna, Y T]]
	[[Category: Poulos, T L]]		[[Category: Poulos, T L]]
	[[Category: Protein in the absence of substrate]]		[[Category: Protein in the absence of substrate]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 08:11, 31 October 2018

OCA — Wed, 31 Oct 2018 08:11:41 GMT

←Older revision		Revision as of 08:11, 31 October 2018
Line 1:		Line 1:
		+
	==The Role of Asn 242 in P450cin==		==The Role of Asn 242 in P450cin==
	<StructureSection load='3bdz' size='340' side='right' caption='[[3bdz]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3bdz' size='340' side='right' caption='[[3bdz]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2b\|1t2b]], [[3be0\|3be0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2b\|1t2b]], [[3be0\|3be0]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cinA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 ATCC 51113])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cinA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 ATCC 51113])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bdz OCA], [http://pdbe.org/3bdz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bdz RCSB], [http://www.ebi.ac.uk/pdbsum/3bdz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bdz OCA], [http://pdbe.org/3bdz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bdz RCSB], [http://www.ebi.ac.uk/pdbsum/3bdz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bdz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bd/3bdz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bd/3bdz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 13:39, 8 February 2016

OCA — Mon, 08 Feb 2016 13:39:39 GMT

←Older revision		Revision as of 13:39, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='3bdz' size='340' side='right' caption='[[3bdz]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3bdz' size='340' side='right' caption='[[3bdz]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bdz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Citrobacter_braakii Citrobacter braakii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BDZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bdz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_51113 Atcc 51113]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BDZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2b\|1t2b]], [[3be0\|3be0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2b\|1t2b]], [[3be0\|3be0]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cinA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 ~~Citrobacter braakii~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cinA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 ATCC 51113])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bdz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bdz RCSB], [http://www.ebi.ac.uk/pdbsum/3bdz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bdz OCA], [http://pdbe.org/3bdz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bdz RCSB], [http://www.ebi.ac.uk/pdbsum/3bdz PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q8VQF6_CITBR Q8VQF6_CITBR~~]] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.	+	[[http://www.uniprot.org/uniprot/CINA_CITBR CINA_CITBR]] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bdz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3bdz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Citrobacter braakii~~]]	+	[[Category: Atcc 51113]]
	[[Category: Meharenna, Y T]]		[[Category: Meharenna, Y T]]
	[[Category: Poulos, T L]]		[[Category: Poulos, T L]]
	[[Category: Protein in the absence of substrate]]		[[Category: Protein in the absence of substrate]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 23:43, 24 December 2014

OCA — Wed, 24 Dec 2014 23:43:30 GMT

←Older revision		Revision as of 23:43, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3bdz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BDZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3bdz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BDZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2b\|1t2b]], [[3be0\|3be0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2b\|1t2b]], [[3be0\|3be0]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cinA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 Citrobacter braakii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cinA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 Citrobacter braakii])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bdz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bdz RCSB], [http://www.ebi.ac.uk/pdbsum/3bdz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bdz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bdz RCSB], [http://www.ebi.ac.uk/pdbsum/3bdz PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q8VQF6_CITBR Q8VQF6_CITBR]] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Citrobacter braakii]]		[[Category: Citrobacter braakii]]
-	[[Category: Meharenna, Y T.]]	+	[[Category: Meharenna, Y T]]
-	[[Category: Poulos, T L.]]	+	[[Category: Poulos, T L]]
	[[Category: Protein in the absence of substrate]]		[[Category: Protein in the absence of substrate]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 09:12, 29 September 2014

OCA — Mon, 29 Sep 2014 09:12:10 GMT

←Older revision		Revision as of 09:12, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3bdz.~~png~~\|~~left~~\|200px]]	+	==The Role of Asn 242 in P450cin==
		+	<StructureSection load='3bdz' size='340' side='right' caption='[[3bdz]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3bdz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BDZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2b\|1t2b]], [[3be0\|3be0]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cinA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 Citrobacter braakii])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bdz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bdz RCSB], [http://www.ebi.ac.uk/pdbsum/3bdz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bd/3bdz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytochrome P450cin (CYP176A1) is a bacterial P450 isolated from Citrobacter braakii that catalyzes the hydroxylation of cineole to (S)-6beta-hydroxycineole. This initiates the biodegradation of cineole, enabling C. braakii to live on cineole as its sole source of carbon and energy. P450cin lacks the almost universally conserved threonine residue believed to be involved in dioxygen activation and instead contains an asparagine at this position (Asn-242). To investigate the role of Asn-242 in P450cin catalysis, it was converted to alanine, and the resultant mutant was characterized. The characteristic CO-bound spectrum and spectrally determined K(D) for substrate binding were unchanged in the mutant. The x-ray crystal structures of the substrate-free and -bound N242A mutant were determined and show that the only significant change is in a reorientation of the substrate such that (R)-6alpha-hydroxycineole should be a major product. Molecular dynamics simulations of both wild type and mutant are consistent with the change in regio- and stereoselectivity predicted from the crystal structure. The mutation has only a modest effect on enzyme activity and on the diversion of the NADPH-reducing equivalent toward unproductive peroxide formation. Product profile analysis shows that (R)-6alpha-hydroxycineole is the main product, which is consistent with the crystal structure. These results demonstrate that Asn-242 is not a functional replacement for the conserved threonine in other P450s but, rather, is critical in controlling regioselective substrate oxidation.

-	~~{{STRUCTURE_3bdz\| PDB=3bdz \| SCENE= }}~~	+	The critical role of substrate-protein hydrogen bonding in the control of regioselective hydroxylation in p450cin.,Meharenna YT, Slessor KE, Cavaignac SM, Poulos TL, De Voss JJ J Biol Chem. 2008 Apr 18;283(16):10804-12. Epub 2008 Feb 12. PMID:18270198<ref>PMID:18270198</ref>

-	~~===The Role~~ of ~~Asn 242 in P450cin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18270198}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3bdz]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Citrobacter_braakii Citrobacter braakii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA].~~	+

	==See Also==		==See Also==
	*[[Cytochrome P450\|Cytochrome P450]]		*[[Cytochrome P450\|Cytochrome P450]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018270198<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Citrobacter braakii]]		[[Category: Citrobacter braakii]]
	[[Category: Meharenna, Y T.]]		[[Category: Meharenna, Y T.]]

OCA at 10:20, 20 October 2012

OCA — Sat, 20 Oct 2012 10:20:10 GMT

←Older revision		Revision as of 10:20, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3bdz]] is a 2 chain structure ~~of [[Cytochrome P450]]~~ with sequence from [http://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA].	+	[[3bdz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA].

	==See Also==		==See Also==

OCA: Protected "3bdz" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 01:46:05 GMT

Protected "3bdz" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:46, 26 July 2012

OCA at 01:46, 26 July 2012

OCA — Thu, 26 Jul 2012 01:46:04 GMT

←Older revision		Revision as of 01:46, 26 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3bdz.png\|left\|200px]]		[[Image:3bdz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3bdz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3bdz\| PDB=3bdz \| SCENE= }}		{{STRUCTURE_3bdz\| PDB=3bdz \| SCENE= }}

	===The Role of Asn 242 in P450cin===		===The Role of Asn 242 in P450cin===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18270198}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18270198 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18270198}}		{{ABSTRACT_PUBMED_18270198}}

	==About this Structure==		==About this Structure==
-	~~3BDZ~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA].	+	[[3bdz]] is a 2 chain structure of [[Cytochrome P450]] with sequence from [http://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA].
		+
		+	==See Also==
		+	*[[Cytochrome P450\|Cytochrome P450]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18270198~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018270198</ref><references group="xtra"/>
	[[Category: Citrobacter braakii]]		[[Category: Citrobacter braakii]]
	[[Category: Meharenna, Y T.]]		[[Category: Meharenna, Y T.]]
Line 29:		Line 20:
	[[Category: Protein in the absence of substrate]]		[[Category: Protein in the absence of substrate]]
	[[Category: Unknown function]]		[[Category: Unknown function]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:50:34 2009''

OCA at 20:50, 16 February 2009

OCA — Mon, 16 Feb 2009 20:50:38 GMT

←Older revision		Revision as of 20:50, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3BDZ is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA].	+	3BDZ is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDZ OCA].

	==Reference==		==Reference==
-	The critical role of substrate-protein hydrogen bonding in the control of regioselective hydroxylation in p450cin., Meharenna YT, Slessor KE, Cavaignac SM, Poulos TL, De Voss JJ, J Biol Chem. 2008 Apr 18;283(16):10804-12. Epub 2008 Feb 12. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18270198 18270198]~~	+	<ref group="xtra">PMID:18270198</ref><references group="xtra"/>
	[[Category: Citrobacter braakii]]		[[Category: Citrobacter braakii]]
-	[[Category: Single protein]]
	[[Category: Meharenna, Y T.]]		[[Category: Meharenna, Y T.]]
	[[Category: Poulos, T L.]]		[[Category: Poulos, T L.]]
Line 31:		Line 30:
	[[Category: Unknown function]]		[[Category: Unknown function]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon ~~Jul 28 03~~:32:~~57 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:50:34 2009''