3bjt - Revision history

OCA at 09:29, 21 February 2024

OCA — Wed, 21 Feb 2024 09:29:30 GMT

←Older revision		Revision as of 09:29, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3bjt' size='340' side='right'caption='[[3bjt]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3bjt' size='340' side='right'caption='[[3bjt]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bjt]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3BJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bjt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BJT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http:~~/~~/en.wikipedia.org/wiki/Pyruvate_kinase Pyruvate kinase]~~, ~~with EC number [http~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.40 2.7.1.40]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3bjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bjt OCA], [~~http~~://pdbe.org/3bjt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3bjt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3bjt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3bjt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bjt OCA], [https://pdbe.org/3bjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bjt RCSB], [https://www.ebi.ac.uk/pdbsum/3bjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/KPYM_HUMAN KPYM_HUMAN]] Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.<ref>PMID:17308100</ref> <ref>PMID:18191611</ref> <ref>PMID:21620138</ref>	+	[https://www.uniprot.org/uniprot/KPYM_HUMAN KPYM_HUMAN] Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.<ref>PMID:17308100</ref> <ref>PMID:18191611</ref> <ref>PMID:21620138</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bjt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bjt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Growth factors stimulate cells to take up excess nutrients and to use them for anabolic processes. The biochemical mechanism by which this is accomplished is not fully understood but it is initiated by phosphorylation of signalling proteins on tyrosine residues. Using a novel proteomic screen for phosphotyrosine-binding proteins, we have made the observation that an enzyme involved in glycolysis, the human M2 (fetal) isoform of pyruvate kinase (PKM2), binds directly and selectively to tyrosine-phosphorylated peptides. We show that binding of phosphotyrosine peptides to PKM2 results in release of the allosteric activator fructose-1,6-bisphosphate, leading to inhibition of PKM2 enzymatic activity. We also provide evidence that this regulation of PKM2 by phosphotyrosine signalling diverts glucose metabolites from energy production to anabolic processes when cells are stimulated by certain growth factors. Collectively, our results indicate that expression of this phosphotyrosine-binding form of pyruvate kinase is critical for rapid growth in cancer cells.
-
-	Pyruvate kinase M2 is a phosphotyrosine-binding protein.,Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC Nature. 2008 Mar 13;452(7184):181-6. PMID:18337815<ref>PMID:18337815</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3bjt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 38:		Line 29:
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Pyruvate kinase]]~~	+	[[Category: Wu N]]
-	[[Category: Wu, N]]	+
-	~~[[Category: Acetylation]]~~	+
-	~~[[Category: Allosteric enzyme]]~~	+
-	~~[[Category: Alternative splicing]]~~	+
-	~~[[Category: Glycolysis]]~~	+
-	~~[[Category: Magnesium]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Polymorphism]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:18, 5 August 2020

OCA — Wed, 05 Aug 2020 06:18:11 GMT

←Older revision		Revision as of 06:18, 5 August 2020
Line 1:		Line 1:

	==Pyruvate kinase M2 is a phosphotyrosine binding protein==		==Pyruvate kinase M2 is a phosphotyrosine binding protein==
-	<StructureSection load='3bjt' size='340' side='right' caption='[[3bjt]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3bjt' size='340' side='right'caption='[[3bjt]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJT OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3BJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3BJT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_kinase Pyruvate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.40 2.7.1.40] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_kinase Pyruvate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.40 2.7.1.40] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3bjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bjt OCA], [http://pdbe.org/3bjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bjt RCSB], [http://www.ebi.ac.uk/pdbsum/3bjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bjt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3bjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bjt OCA], [http://pdbe.org/3bjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bjt RCSB], [http://www.ebi.ac.uk/pdbsum/3bjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Pyruvate ~~Kinase~~\|Pyruvate ~~Kinase~~]]	+	*[[Pyruvate kinase 3D structures\|Pyruvate kinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
	[[Category: Pyruvate kinase]]		[[Category: Pyruvate kinase]]
	[[Category: Wu, N]]		[[Category: Wu, N]]

OCA at 07:46, 31 October 2018

OCA — Wed, 31 Oct 2018 07:46:58 GMT

←Older revision		Revision as of 07:46, 31 October 2018
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/3bjt_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/3bjt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 39:		Line 39:
	[[Category: Pyruvate kinase]]		[[Category: Pyruvate kinase]]
	[[Category: Wu, N]]		[[Category: Wu, N]]
		+	[[Category: Acetylation]]
	[[Category: Allosteric enzyme]]		[[Category: Allosteric enzyme]]
		+	[[Category: Alternative splicing]]
	[[Category: Glycolysis]]		[[Category: Glycolysis]]
	[[Category: Magnesium]]		[[Category: Magnesium]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
		+	[[Category: Polymorphism]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 05:39, 5 August 2016

OCA — Fri, 05 Aug 2016 05:39:31 GMT

←Older revision		Revision as of 05:39, 5 August 2016
Line 1:		Line 1:
		+
	==Pyruvate kinase M2 is a phosphotyrosine binding protein==		==Pyruvate kinase M2 is a phosphotyrosine binding protein==
	<StructureSection load='3bjt' size='340' side='right' caption='[[3bjt]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3bjt' size='340' side='right' caption='[[3bjt]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_kinase Pyruvate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.40 2.7.1.40] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_kinase Pyruvate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.40 2.7.1.40] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bjt RCSB], [http://www.ebi.ac.uk/pdbsum/3bjt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bjt OCA], [http://pdbe.org/3bjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bjt RCSB], [http://www.ebi.ac.uk/pdbsum/3bjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bjt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3bjt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 16:12, 25 December 2014

OCA — Thu, 25 Dec 2014 16:12:24 GMT

←Older revision		Revision as of 16:12, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3bjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BJT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3bjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BJT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_kinase Pyruvate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.40 2.7.1.40] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_kinase Pyruvate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.40 2.7.1.40] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bjt RCSB], [http://www.ebi.ac.uk/pdbsum/3bjt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bjt RCSB], [http://www.ebi.ac.uk/pdbsum/3bjt PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KPYM_HUMAN KPYM_HUMAN]] Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.<ref>PMID:17308100</ref> <ref>PMID:18191611</ref> <ref>PMID:21620138</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Pyruvate kinase]]		[[Category: Pyruvate kinase]]
-	[[Category: Wu, N.]]	+	[[Category: Wu, N]]
	[[Category: Allosteric enzyme]]		[[Category: Allosteric enzyme]]
	[[Category: Glycolysis]]		[[Category: Glycolysis]]
Line 40:		Line 42:
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
-	[[Category: Pyruvate kinase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 21:02, 2 October 2014

OCA — Thu, 02 Oct 2014 21:02:29 GMT

←Older revision		Revision as of 21:02, 2 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3bjt~~\| ~~PDB~~=3bjt \| ~~SCENE~~= }}	+	==Pyruvate kinase M2 is a phosphotyrosine binding protein==
-	===Pyruvate kinase ~~M2 is a phosphotyrosine binding~~ protein===	+	<StructureSection load='3bjt' size='340' side='right' caption='[[3bjt]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_18337815}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3bjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BJT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_kinase Pyruvate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.40 2.7.1.40] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bjt RCSB], [http://www.ebi.ac.uk/pdbsum/3bjt PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/3bjt_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Growth factors stimulate cells to take up excess nutrients and to use them for anabolic processes. The biochemical mechanism by which this is accomplished is not fully understood but it is initiated by phosphorylation of signalling proteins on tyrosine residues. Using a novel proteomic screen for phosphotyrosine-binding proteins, we have made the observation that an enzyme involved in glycolysis, the human M2 (fetal) isoform of pyruvate kinase (PKM2), binds directly and selectively to tyrosine-phosphorylated peptides. We show that binding of phosphotyrosine peptides to PKM2 results in release of the allosteric activator fructose-1,6-bisphosphate, leading to inhibition of PKM2 enzymatic activity. We also provide evidence that this regulation of PKM2 by phosphotyrosine signalling diverts glucose metabolites from energy production to anabolic processes when cells are stimulated by certain growth factors. Collectively, our results indicate that expression of this phosphotyrosine-binding form of pyruvate kinase is critical for rapid growth in cancer cells.

-	~~==About this Structure==~~	+	Pyruvate kinase M2 is a phosphotyrosine-binding protein.,Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC Nature. 2008 Mar 13;452(7184):181-6. PMID:18337815<ref>PMID:18337815</ref>
-	~~[[3bjt]]~~ is a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3BJT OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Pyruvate Kinase\|Pyruvate Kinase]]		*[[Pyruvate Kinase\|Pyruvate Kinase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018337815<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Pyruvate kinase]]		[[Category: Pyruvate kinase]]

OCA at 10:21, 11 March 2013

OCA — Mon, 11 Mar 2013 10:21:56 GMT

←Older revision		Revision as of 10:21, 11 March 2013
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-	[[Image:3bjt.png\|left\|200px]]
-
	{{STRUCTURE_3bjt\| PDB=3bjt \| SCENE= }}		{{STRUCTURE_3bjt\| PDB=3bjt \| SCENE= }}
-
	===Pyruvate kinase M2 is a phosphotyrosine binding protein===		===Pyruvate kinase M2 is a phosphotyrosine binding protein===
-
	{{ABSTRACT_PUBMED_18337815}}		{{ABSTRACT_PUBMED_18337815}}

OCA: Protected "3bjt" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 19:22:58 GMT

Protected "3bjt" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:22, 7 January 2013

OCA at 19:22, 7 January 2013

OCA — Mon, 07 Jan 2013 19:22:56 GMT

←Older revision		Revision as of 19:22, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3bjt.png\|left\|200px]]		[[Image:3bjt.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3bjt", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3bjt\| PDB=3bjt \| SCENE= }}		{{STRUCTURE_3bjt\| PDB=3bjt \| SCENE= }}

	===Pyruvate kinase M2 is a phosphotyrosine binding protein===		===Pyruvate kinase M2 is a phosphotyrosine binding protein===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18337815}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18337815 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18337815}}		{{ABSTRACT_PUBMED_18337815}}

	==About this Structure==		==About this Structure==
-	~~3BJT~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJT OCA].	+	[[3bjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJT OCA].
		+
		+	==See Also==
		+	*[[Pyruvate Kinase\|Pyruvate Kinase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18337815~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018337815</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Pyruvate kinase]]		[[Category: Pyruvate kinase]]
	[[Category: Wu, N.]]		[[Category: Wu, N.]]
-	[[Category: Acetylation]]
	[[Category: Allosteric enzyme]]		[[Category: Allosteric enzyme]]
-	[[Category: Alternative splicing]]
	[[Category: Glycolysis]]		[[Category: Glycolysis]]
	[[Category: Magnesium]]		[[Category: Magnesium]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
-	[[Category: Polymorphism]]
	[[Category: Pyruvate kinase]]		[[Category: Pyruvate kinase]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 22 10:54:34 2009''

OCA at 08:54, 22 March 2009

OCA — Sun, 22 Mar 2009 08:54:35 GMT

←Older revision		Revision as of 08:54, 22 March 2009
Line 12:		Line 12:
	===Pyruvate kinase M2 is a phosphotyrosine binding protein===		===Pyruvate kinase M2 is a phosphotyrosine binding protein===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18337815}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18337815 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18337815}}

	==About this Structure==		==About this Structure==
	3BJT is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJT OCA].		3BJT is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJT OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:18337815</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Pyruvate kinase]]		[[Category: Pyruvate kinase]]
Line 29:		Line 38:
	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Feb 17 21~~:25:42 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 22 10:54:34 2009''