3bkh - Revision history

OCA at 10:45, 12 January 2022

OCA — Wed, 12 Jan 2022 10:45:08 GMT

←Older revision		Revision as of 10:45, 12 January 2022
Line 1:		Line 1:

	==Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144==		==Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144==
-	<StructureSection load='3bkh' size='340' side='right' caption='[[3bkh]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3bkh' size='340' side='right'caption='[[3bkh]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bkh]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bpdpk Bpdpk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BKH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3BKH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bkh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpdpk Bpdpk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BKH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bkv\|3bkv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bkv\|3bkv]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ORF144 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169683 BPDPK])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ORF144 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169683 BPDPK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3bkh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bkh OCA], [~~http~~://pdbe.org/3bkh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3bkh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3bkh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3bkh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bkh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bkh OCA], [https://pdbe.org/3bkh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bkh RCSB], [https://www.ebi.ac.uk/pdbsum/3bkh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bkh ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Bpdpk]]		[[Category: Bpdpk]]
		+	[[Category: Large Structures]]
	[[Category: Fokine, A]]		[[Category: Fokine, A]]
	[[Category: Mesyanzhinov, V V]]		[[Category: Mesyanzhinov, V V]]

OCA at 07:42, 31 October 2018

OCA — Wed, 31 Oct 2018 07:42:54 GMT

←Older revision		Revision as of 07:42, 31 October 2018
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/3bkh_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/3bkh_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 15:14, 11 August 2016

OCA — Thu, 11 Aug 2016 15:14:17 GMT

←Older revision		Revision as of 15:14, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144==		==Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144==
	<StructureSection load='3bkh' size='340' side='right' caption='[[3bkh]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3bkh' size='340' side='right' caption='[[3bkh]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bkh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_phage_phikz Pseudomonas phage phikz~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BKH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BKH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bkh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpdpk Bpdpk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BKH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BKH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bkv\|3bkv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bkv\|3bkv]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ORF144 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169683 ~~Pseudomonas phage phiKZ~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ORF144 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169683 BPDPK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bkh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bkh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bkh RCSB], [http://www.ebi.ac.uk/pdbsum/3bkh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bkh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bkh OCA], [http://pdbe.org/3bkh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bkh RCSB], [http://www.ebi.ac.uk/pdbsum/3bkh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bkh ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bkh ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3bkh" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseudomonas phage phikz~~]]	+	[[Category: Bpdpk]]
	[[Category: Fokine, A]]		[[Category: Fokine, A]]
	[[Category: Mesyanzhinov, V V]]		[[Category: Mesyanzhinov, V V]]

OCA at 09:05, 20 January 2015

OCA — Tue, 20 Jan 2015 09:05:24 GMT

←Older revision		Revision as of 09:05, 20 January 2015
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Pseudomonas phage phikz]]		[[Category: Pseudomonas phage phikz]]
-	[[Category: Fokine, A.]]	+	[[Category: Fokine, A]]
-	[[Category: Mesyanzhinov, V V.]]	+	[[Category: Mesyanzhinov, V V]]
-	[[Category: Miroshnikov, K A.]]	+	[[Category: Miroshnikov, K A]]
-	[[Category: Rossmann, M G.]]	+	[[Category: Rossmann, M G]]
-	[[Category: Shneider, M M.]]	+	[[Category: Shneider, M M]]
	[[Category: Bacteriophage]]		[[Category: Bacteriophage]]
	[[Category: Cell wall degradation]]		[[Category: Cell wall degradation]]

OCA at 06:29, 10 October 2014

OCA — Fri, 10 Oct 2014 06:29:53 GMT

←Older revision		Revision as of 06:29, 10 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3bkh\| PDB=3bkh \| SCENE= }}~~	+	==Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144==
-	===Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144===	+	<StructureSection load='3bkh' size='340' side='right' caption='[[3bkh]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_18160394}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3bkh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phikz Pseudomonas phage phikz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BKH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BKH FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bkv\|3bkv]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ORF144 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169683 Pseudomonas phage phiKZ])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bkh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bkh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bkh RCSB], [http://www.ebi.ac.uk/pdbsum/3bkh PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/3bkh_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lytic transglycosylases are enzymes that act on the peptidoglycan of bacterial cell walls. They cleave the glycosidic linkage between N-acetylmuramoyl and N-acetylglucosaminyl residues with the concomitant formation of a 1,6-anhydromuramoyl product. The x-ray structure of the lytic transglycosylase gp144 from the Pseudomonas bacteriophage phi KZ has been determined to 2.5-A resolution. This protein is probably employed by the bacteriophage in the late stage of the virus reproduction cycle to destroy the bacterial cell wall to release the phage progeny. phi KZ gp144 is a 260-residue alpha-helical protein composed of a 70-residue N-terminal cell wall-binding domain and a C-terminal catalytic domain. The fold of the N-terminal domain is similar to the peptidoglycan-binding domain from Streptomyces albus G D-Ala-D-Ala carboxypeptidase and to the N-terminal prodomain of human metalloproteinases that act on extracellular matrices. The C-terminal catalytic domain of gp144 has a structural similarity to the catalytic domain of the transglycosylase Slt70 from Escherichia coli and to lysozymes. The gp144 catalytic domain has an elongated groove that can bind at least five sugar residues at sites A-E. As in other lysozymes, the peptidoglycan cleavage (catalyzed by Glu 115 in gp144) occurs between sugar-binding subsites D and E. The x-ray structure of the phi KZ transglycosylase complexed with the chitotetraose (N-acetylglucosamine)(4) has been determined to 2.6-A resolution. The N-acetylglucosamine residues of the chitotetraose bind in sites A-D.

-	~~==About this~~ Structure==	+	Structure of the bacteriophage phi KZ lytic transglycosylase gp144.,Fokine A, Miroshnikov KA, Shneider MM, Mesyanzhinov VV, Rossmann MG J Biol Chem. 2008 Mar 14;283(11):7242-50. Epub 2007 Dec 26. PMID:18160394<ref>PMID:18160394</ref>
-	~~[[3bkh]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_phage_phikz Pseudomonas phage phikz]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3BKH OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:018160394</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Pseudomonas phage phikz]]		[[Category: Pseudomonas phage phikz]]
	[[Category: Fokine, A.]]		[[Category: Fokine, A.]]

OCA at 07:40, 30 June 2013

OCA — Sun, 30 Jun 2013 07:40:20 GMT

←Older revision		Revision as of 07:40, 30 June 2013
Line 1:		Line 1:
-	[[Image:3bkh.png\|left\|200px]]
-
	{{STRUCTURE_3bkh\| PDB=3bkh \| SCENE= }}		{{STRUCTURE_3bkh\| PDB=3bkh \| SCENE= }}
-
	===Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144===		===Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144===
-
	{{ABSTRACT_PUBMED_18160394}}		{{ABSTRACT_PUBMED_18160394}}

Line 11:		Line 7:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:018160394</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018160394</ref><references group="xtra"/><references/>
	[[Category: Pseudomonas phage phikz]]		[[Category: Pseudomonas phage phikz]]
	[[Category: Fokine, A.]]		[[Category: Fokine, A.]]

OCA: Protected "3bkh" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 18:36:52 GMT

Protected "3bkh" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:36, 7 January 2013

OCA at 18:36, 7 January 2013

OCA — Mon, 07 Jan 2013 18:36:49 GMT

←Older revision		Revision as of 18:36, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3bkh.png\|left\|200px]]		[[Image:3bkh.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3bkh", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3bkh\| PDB=3bkh \| SCENE= }}		{{STRUCTURE_3bkh\| PDB=3bkh \| SCENE= }}

	===Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144===		===Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18160394}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18160394 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18160394}}		{{ABSTRACT_PUBMED_18160394}}

	==About this Structure==		==About this Structure==
-	~~3BKH~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phikz Pseudomonas phage phikz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BKH OCA].	+	[[3bkh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phikz Pseudomonas phage phikz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BKH OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18160394~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018160394</ref><references group="xtra"/>
	[[Category: Pseudomonas phage phikz]]		[[Category: Pseudomonas phage phikz]]
	[[Category: Fokine, A.]]		[[Category: Fokine, A.]]
Line 38:		Line 26:
	[[Category: Phikz]]		[[Category: Phikz]]
	[[Category: Transglycosylase]]		[[Category: Transglycosylase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:45:50 2009''

OCA at 02:45, 18 February 2009

OCA — Wed, 18 Feb 2009 02:45:51 GMT

←Older revision		Revision as of 02:45, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3BKH is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phikz Pseudomonas phage phikz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BKH OCA].	+	3BKH is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phikz Pseudomonas phage phikz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BKH OCA].

	==Reference==		==Reference==
-	~~Structure of the bacteriophage phiKZ lytic transglycosylase, gp144., Fokine A, Miroshnikov KA, Shneider MM, Mesyanzhinov VV, Rossmann MG, J Biol Chem. 2007 Dec 26;.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18160394 18160394]~~	+	<ref group="xtra">PMID:18160394</ref><references group="xtra"/>
	[[Category: Pseudomonas phage phikz]]		[[Category: Pseudomonas phage phikz]]
-	[[Category: Single protein]]
	[[Category: Fokine, A.]]		[[Category: Fokine, A.]]
	[[Category: Mesyanzhinov, V V.]]		[[Category: Mesyanzhinov, V V.]]
Line 40:		Line 39:
	[[Category: Transglycosylase]]		[[Category: Transglycosylase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jul 23 10~~:02:~~21 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:45:50 2009''

OCA at 07:02, 23 July 2008

OCA — Wed, 23 Jul 2008 07:02:21 GMT

←Older revision		Revision as of 07:02, 23 July 2008
Line 1:		Line 1:
-	[[Image:3bkh.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3bkh.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_3bkh\| PDB=3bkh \| SCENE= }}		{{STRUCTURE_3bkh\| PDB=3bkh \| SCENE= }}

-	~~'''~~Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144~~'''~~	+	===Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144===


-	~~==Overview==~~	+	<!--
-	~~Lytic transglycosylases are enzymes which act on the peptidoglycan of bacterial cell walls. They cleave the glycosidic linkage between N~~-~~acetylmuramoyl and N~~-~~acetylglucosaminyl residues with the concomitant formation of a 1,6-anhydromuramoyl product.~~ The ~~X-ray structure of the lytic transglycosylase~~, ~~gp144~~, ~~from~~ the ~~Pseudomonas bacteriophage phiKZ, has been determined~~ to ~~2.5 A resolution. This protein is probably employed by~~ the ~~bacteriophage in the late stage of the virus reproduction cycle to destroy the bacterial cell wall to release the phage progeny~~. ~~The phiKZ gp144 is a 260 residue a-helical protein composed of a 70-residue N-terminal cell wall binding domain and a C-terminal catalytic domain~~. ~~The fold of the N-terminal domain~~ is ~~similar to~~ the ~~peptidoglycan binding domain from Streptomyces albus G D-Ala-D-Ala carboxypeptidase and to the N-terminal pro-domain of human metalloproteinases that act on extracellular matrices~~. ~~The C~~-terminal catalytic domain of gp144 has a structural similarity to the catalytic domain of the transglycosylase SLT70 from E. coli and to lysozymes. The gp144 catalytic domain has an elongated groove which can bind at least five sugar residues at sites A, B, C, D, and E. As in other lysozymes, the peptidoglycan cleavage, catalyzed by Glu 115 in gp144, occurs between the sugar-binding subsites D and E. The X-ray structure of the phiKZ transglycosylase has been determined to 2.6 A resolution when complexed with the chitotetraose (NAG)(4). The NAG residues of the chitotetraose bind in sites A through D.	+	The line below this paragraph, {{ABSTRACT_PUBMED_18160394}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18160394 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18160394}}

	==About this Structure==		==About this Structure==
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	[[Category: Phikz]]		[[Category: Phikz]]
	[[Category: Transglycosylase]]		[[Category: Transglycosylase]]
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