3bzn - Revision history

OCA at 09:32, 21 February 2024

OCA — Wed, 21 Feb 2024 09:32:00 GMT

←Older revision		Revision as of 09:32, 21 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3bzn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BZN FirstGlance]. <br>		<table><tr><td colspan='2'>[[3bzn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BZN FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3bzm\|3bzm]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>menF, yfbA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562 Escherichia coli])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzn OCA], [https://pdbe.org/3bzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bzn RCSB], [https://www.ebi.ac.uk/pdbsum/3bzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzn OCA], [https://pdbe.org/3bzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bzn RCSB], [https://www.ebi.ac.uk/pdbsum/3bzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MENF_ECOLI MENF_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bzn ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bzn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The electron carrier menaquinone is one of many important bacterial metabolites that are derived from the key intermediate chorismic acid. MenF, the first enzyme in the menaquinone pathway, catalyzes the isomerization of chorismate to isochorismate. Here, an improved structure of MenF in a new crystal form is presented. The structure, solved at 2.0 angstroms resolution in complex with magnesium, reveals a well defined closed active site. Existing evidence suggests that the mechanism of the reaction catalyzed by MenF involves nucleophilic attack of a water molecule on the chorismate ring. The structure reveals a well defined water molecule located in an appropriate position for activation by Lys190 and attack on the substrate.
-
-	Structure of isochorismate synthase in complex with magnesium.,Parsons JF, Shi KM, Ladner JE Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):607-10. Epub 2008, Apr 19. PMID:18453696<ref>PMID:18453696</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3bzn" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Isochorismate synthase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ladner~~, J E~~]]	+	[[Category: Ladner JE]]
-	[[Category: Parsons~~, J F~~]]	+	[[Category: Parsons JF]]
-	[[Category: Shi~~, K M]]~~	+	[[Category: Shi KM]]
-	~~[[Category: Chorismate]]~~	+
-	~~[[Category: Isochorismate]]~~	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Menaquinone]]~~	+
-	~~[[Category: Menaquinone biosynthesis~~]]	+

OCA at 07:37, 27 January 2022

OCA — Thu, 27 Jan 2022 07:37:48 GMT

←Older revision		Revision as of 07:37, 27 January 2022
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	==Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase, MenF==		==Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase, MenF==
-	<StructureSection load='3bzn' size='340' side='right' caption='[[3bzn]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3bzn' size='340' side='right'caption='[[3bzn]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bzn]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3BZN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bzn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BZN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bzm\|3bzm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bzm\|3bzm]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menF, yfbA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menF, yfbA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3bzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzn OCA], [~~http~~://pdbe.org/3bzn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3bzn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3bzn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzn OCA], [https://pdbe.org/3bzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bzn RCSB], [https://www.ebi.ac.uk/pdbsum/3bzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 35:		Line 35:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Isochorismate synthase]]		[[Category: Isochorismate synthase]]
		+	[[Category: Large Structures]]
	[[Category: Ladner, J E]]		[[Category: Ladner, J E]]
	[[Category: Parsons, J F]]		[[Category: Parsons, J F]]

OCA at 13:15, 7 November 2018

OCA — Wed, 07 Nov 2018 13:15:14 GMT

←Older revision		Revision as of 13:15, 7 November 2018
Line 1:		Line 1:
		+
	==Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase, MenF==		==Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase, MenF==
	<StructureSection load='3bzn' size='340' side='right' caption='[[3bzn]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3bzn' size='340' side='right' caption='[[3bzn]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menF, yfbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menF, yfbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzn OCA], [http://pdbe.org/3bzn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bzn RCSB], [http://www.ebi.ac.uk/pdbsum/3bzn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzn OCA], [http://pdbe.org/3bzn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bzn RCSB], [http://www.ebi.ac.uk/pdbsum/3bzn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/3bzn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/3bzn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 10:42, 8 February 2016

OCA — Mon, 08 Feb 2016 10:42:02 GMT

←Older revision		Revision as of 10:42, 8 February 2016
Line 7:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menF, yfbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menF, yfbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bzn RCSB], [http://www.ebi.ac.uk/pdbsum/3bzn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzn OCA], [http://pdbe.org/3bzn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bzn RCSB], [http://www.ebi.ac.uk/pdbsum/3bzn PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bzn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3bzn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:54, 20 January 2015

OCA — Tue, 20 Jan 2015 08:54:22 GMT

←Older revision		Revision as of 08:54, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3bzn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BZN FirstGlance]. <br>		<table><tr><td colspan='2'>[[3bzn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BZN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bzm\|3bzm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bzm\|3bzm]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menF, yfbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menF, yfbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bzn RCSB], [http://www.ebi.ac.uk/pdbsum/3bzn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bzn RCSB], [http://www.ebi.ac.uk/pdbsum/3bzn PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Isochorismate synthase]]		[[Category: Isochorismate synthase]]
-	[[Category: Ladner, J E.]]	+	[[Category: Ladner, J E]]
-	[[Category: Parsons, J F.]]	+	[[Category: Parsons, J F]]
-	[[Category: Shi, K M.]]	+	[[Category: Shi, K M]]
	[[Category: Chorismate]]		[[Category: Chorismate]]
	[[Category: Isochorismate]]		[[Category: Isochorismate]]

OCA at 20:47, 2 October 2014

OCA — Thu, 02 Oct 2014 20:47:48 GMT

←Older revision		Revision as of 20:47, 2 October 2014
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-	[[~~Image:~~3bzn.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase, MenF==
		+	<StructureSection load='3bzn' size='340' side='right' caption='[[3bzn]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3bzn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BZN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bzm\|3bzm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menF, yfbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bzn RCSB], [http://www.ebi.ac.uk/pdbsum/3bzn PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/3bzn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The electron carrier menaquinone is one of many important bacterial metabolites that are derived from the key intermediate chorismic acid. MenF, the first enzyme in the menaquinone pathway, catalyzes the isomerization of chorismate to isochorismate. Here, an improved structure of MenF in a new crystal form is presented. The structure, solved at 2.0 angstroms resolution in complex with magnesium, reveals a well defined closed active site. Existing evidence suggests that the mechanism of the reaction catalyzed by MenF involves nucleophilic attack of a water molecule on the chorismate ring. The structure reveals a well defined water molecule located in an appropriate position for activation by Lys190 and attack on the substrate.

-	~~{{STRUCTURE_3bzn\| PDB=3bzn \| SCENE= }}~~	+	Structure of isochorismate synthase in complex with magnesium.,Parsons JF, Shi KM, Ladner JE Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):607-10. Epub 2008, Apr 19. PMID:18453696<ref>PMID:18453696</ref>

-	~~===Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase~~, ~~MenF===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18453696}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3bzn]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3BZN OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018453696<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Isochorismate synthase]]		[[Category: Isochorismate synthase]]

OCA: Protected "3bzn" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 18:29:22 GMT

Protected "3bzn" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:29, 7 January 2013

OCA at 18:29, 7 January 2013

OCA — Mon, 07 Jan 2013 18:29:16 GMT

←Older revision		Revision as of 18:29, 7 January 2013
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-	{{Seed}}
	[[Image:3bzn.png\|left\|200px]]		[[Image:3bzn.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3bzn\| PDB=3bzn \| SCENE= }}		{{STRUCTURE_3bzn\| PDB=3bzn \| SCENE= }}

	===Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase, MenF===		===Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase, MenF===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18453696}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18453696 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_18453696}}		{{ABSTRACT_PUBMED_18453696}}

	==About this Structure==		==About this Structure==
-	~~3BZN~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZN OCA].	+	[[3bzn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZN OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18453696~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018453696</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Isochorismate synthase]]		[[Category: Isochorismate synthase]]
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	[[Category: Menaquinone]]		[[Category: Menaquinone]]
	[[Category: Menaquinone biosynthesis]]		[[Category: Menaquinone biosynthesis]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:24:54 2009''

OCA at 19:24, 16 February 2009

OCA — Mon, 16 Feb 2009 19:24:56 GMT

←Older revision		Revision as of 19:24, 16 February 2009
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	==About this Structure==		==About this Structure==
-	3BZN is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZN OCA].	+	3BZN is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZN OCA].

	==Reference==		==Reference==
-	~~Structure of isochorismate synthase in complex with magnesium., Parsons JF, Shi KM, Ladner JE, Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):607-10. Epub 2008, Apr 19.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18453696 18453696]~~	+	<ref group="xtra">PMID:18453696</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Isochorismate synthase]]		[[Category: Isochorismate synthase]]
-	[[Category: Single protein]]
	[[Category: Ladner, J E.]]		[[Category: Ladner, J E.]]
	[[Category: Parsons, J F.]]		[[Category: Parsons, J F.]]
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	[[Category: Menaquinone biosynthesis]]		[[Category: Menaquinone biosynthesis]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon ~~Jul 28 02~~:17:~~41 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:24:54 2009''

OCA at 23:17, 27 July 2008

OCA — Sun, 27 Jul 2008 23:17:46 GMT

←Older revision		Revision as of 23:17, 27 July 2008
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-	[[Image:3bzn.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3bzn.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_3bzn\| PDB=3bzn \| SCENE= }}		{{STRUCTURE_3bzn\| PDB=3bzn \| SCENE= }}

-	~~'''~~Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase, MenF~~'''~~	+	===Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase, MenF===


-	~~==Overview==~~	+	<!--
-	The ~~electron carrier menaquinone is one of many important bacterial metabolites that are derived from the key intermediate chorismic acid. MenF~~, ~~the first enzyme in the menaquinone pathway~~, ~~catalyzes~~ the ~~isomerization of chorismate~~ to ~~isochorismate~~. ~~Here~~, ~~an improved structure of MenF in a new crystal form~~ is presented. The structure, solved at 2.0 A resolution in complex with magnesium, reveals a well defined closed active site. Existing evidence suggests that the mechanism of the reaction catalyzed by MenF involves nucleophilic attack of a water molecule on the ~~chorismate ring. The structure reveals a well defined water molecule located in an appropriate position for activation by Lys190 and attack on the substrate~~.	+	The line below this paragraph, {{ABSTRACT_PUBMED_18453696}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18453696 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18453696}}

	==About this Structure==		==About this Structure==
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	[[Category: Menaquinone]]		[[Category: Menaquinone]]
	[[Category: Menaquinone biosynthesis]]		[[Category: Menaquinone biosynthesis]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu May 22 21~~:52:57 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:17:41 2008''