3bzx - Revision history

OCA at 09:32, 21 February 2024

OCA — Wed, 21 Feb 2024 09:32:13 GMT

←Older revision		Revision as of 09:32, 21 February 2024
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	<StructureSection load='3bzx' size='340' side='right'caption='[[3bzx]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='3bzx' size='340' side='right'caption='[[3bzx]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bzx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BZX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bzx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BZX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3bzo\|3bzo]], [[3bzl\|3bzl]], [[3bzp\|3bzp]], [[3bzr\|3bzr]], [[3bzs\|3bzs]], [[3bzt\|3bzt]], [[3bzv\|3bzv]], [[3bzy\|3bzy]], [[3bzz\|3bzz]], [[3c00\|3c00]], [[3c01\|3c01]], [[3c03\|3c03]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">escU ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzx OCA], [https://pdbe.org/3bzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bzx RCSB], [https://www.ebi.ac.uk/pdbsum/3bzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzx OCA], [https://pdbe.org/3bzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bzx RCSB], [https://www.ebi.ac.uk/pdbsum/3bzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzx ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9AJ26_ECOLX Q9AJ26_ECOLX]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bzx ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bzx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	During infection by Gram-negative pathogenic bacteria, the type III secretion system (T3SS) is assembled to allow for the direct transmission of bacterial virulence effectors into the host cell. The T3SS system is characterized by a series of prominent multi-component rings in the inner and outer bacterial membranes, as well as a translocation pore in the host cell membrane. These are all connected by a series of polymerized tubes that act as the direct conduit for the T3SS proteins to pass through to the host cell. During assembly of the T3SS, as well as the evolutionarily related flagellar apparatus, a post-translational cleavage event within the inner membrane proteins EscU/FlhB is required to promote a secretion-competent state. These proteins have long been proposed to act as a part of a molecular switch, which would regulate the appropriate chronological secretion of the various T3SS apparatus components during assembly and subsequently the transported virulence effectors. Here we show that a surface type II beta-turn in the Escherichia coli protein EscU undergoes auto-cleavage by a mechanism involving cyclization of a strictly conserved asparagine residue. Structural and in vivo analysis of point and deletion mutations illustrates the subtle conformational effects of auto-cleavage in modulating the molecular features of a highly conserved surface region of EscU, a potential point of interaction with other T3SS components at the inner membrane. In addition, this work provides new structural insight into the distinct conformational requirements for a large class of self-cleaving reactions involving asparagine cyclization.
-
-	Structural analysis of the essential self-cleaving type III secretion proteins EscU and SpaS.,Zarivach R, Deng W, Vuckovic M, Felise HB, Nguyen HV, Miller SI, Finlay BB, Strynadka NC Nature. 2008 May 1;453(7191):124-7. PMID:18451864<ref>PMID:18451864</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3bzx" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Deng, W]]	+	[[Category: Deng W]]
-	[[Category: Felise~~, H B~~]]	+	[[Category: Felise HB]]
-	[[Category: Finlay~~, B B~~]]	+	[[Category: Finlay BB]]
-	[[Category: Miller~~, S I~~]]	+	[[Category: Miller SI]]
-	[[Category: Nguyen~~, H V~~]]	+	[[Category: Nguyen HV]]
-	[[Category: Strynadka~~, N C.J~~]]	+	[[Category: Strynadka NCJ]]
-	[[Category: Vuckovic, M]]	+	[[Category: Vuckovic M]]
-	[[Category: Zarivach, R]]	+	[[Category: Zarivach R]]
-	~~[[Category: Auto cleavage protein]]~~	+
-	~~[[Category: Intein]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: T3ss~~]]	+

OCA at 19:04, 20 October 2021

OCA — Wed, 20 Oct 2021 19:04:27 GMT

←Older revision		Revision as of 19:04, 20 October 2021
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	==Crystal structure of the mutated H265A EscU C-terminal domain==		==Crystal structure of the mutated H265A EscU C-terminal domain==
-	<StructureSection load='3bzx' size='340' side='right' caption='[[3bzx]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='3bzx' size='340' side='right'caption='[[3bzx]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bzx]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3BZX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bzx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BZX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bzo\|3bzo]], [[3bzl\|3bzl]], [[3bzp\|3bzp]], [[3bzr\|3bzr]], [[3bzs\|3bzs]], [[3bzt\|3bzt]], [[3bzv\|3bzv]], [[3bzy\|3bzy]], [[3bzz\|3bzz]], [[3c00\|3c00]], [[3c01\|3c01]], [[3c03\|3c03]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bzo\|3bzo]], [[3bzl\|3bzl]], [[3bzp\|3bzp]], [[3bzr\|3bzr]], [[3bzs\|3bzs]], [[3bzt\|3bzt]], [[3bzv\|3bzv]], [[3bzy\|3bzy]], [[3bzz\|3bzz]], [[3c00\|3c00]], [[3c01\|3c01]], [[3c03\|3c03]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">escU ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">escU ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3bzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzx OCA], [~~http~~://pdbe.org/3bzx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3bzx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3bzx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzx OCA], [https://pdbe.org/3bzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bzx RCSB], [https://www.ebi.ac.uk/pdbsum/3bzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzx ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/3bzx_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/3bzx_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Deng, W]]		[[Category: Deng, W]]
	[[Category: Felise, H B]]		[[Category: Felise, H B]]

OCA at 11:17, 11 August 2016

OCA — Thu, 11 Aug 2016 11:17:10 GMT

←Older revision		Revision as of 11:17, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the mutated H265A EscU C-terminal domain==		==Crystal structure of the mutated H265A EscU C-terminal domain==
	<StructureSection load='3bzx' size='340' side='right' caption='[[3bzx]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='3bzx' size='340' side='right' caption='[[3bzx]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3bzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BZX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3bzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BZX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bzo\|3bzo]], [[3bzl\|3bzl]], [[3bzp\|3bzp]], [[3bzr\|3bzr]], [[3bzs\|3bzs]], [[3bzt\|3bzt]], [[3bzv\|3bzv]], [[3bzy\|3bzy]], [[3bzz\|3bzz]], [[3c00\|3c00]], [[3c01\|3c01]], [[3c03\|3c03]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bzo\|3bzo]], [[3bzl\|3bzl]], [[3bzp\|3bzp]], [[3bzr\|3bzr]], [[3bzs\|3bzs]], [[3bzt\|3bzt]], [[3bzv\|3bzv]], [[3bzy\|3bzy]], [[3bzz\|3bzz]], [[3c00\|3c00]], [[3c01\|3c01]], [[3c03\|3c03]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">escU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">escU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bzx RCSB], [http://www.ebi.ac.uk/pdbsum/3bzx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzx OCA], [http://pdbe.org/3bzx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bzx RCSB], [http://www.ebi.ac.uk/pdbsum/3bzx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzx ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bzx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3bzx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Deng, W]]		[[Category: Deng, W]]
	[[Category: Felise, H B]]		[[Category: Felise, H B]]

OCA at 08:29, 20 January 2015

OCA — Tue, 20 Jan 2015 08:29:17 GMT

←Older revision		Revision as of 08:29, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3bzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BZX FirstGlance]. <br>		<table><tr><td colspan='2'>[[3bzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BZX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bzo\|3bzo]], [[3bzl\|3bzl]], [[3bzp\|3bzp]], [[3bzr\|3bzr]], [[3bzs\|3bzs]], [[3bzt\|3bzt]], [[3bzv\|3bzv]], [[3bzy\|3bzy]], [[3bzz\|3bzz]], [[3c00\|3c00]], [[3c01\|3c01]], [[3c03\|3c03]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bzo\|3bzo]], [[3bzl\|3bzl]], [[3bzp\|3bzp]], [[3bzr\|3bzr]], [[3bzs\|3bzs]], [[3bzt\|3bzt]], [[3bzv\|3bzv]], [[3bzy\|3bzy]], [[3bzz\|3bzz]], [[3c00\|3c00]], [[3c01\|3c01]], [[3c03\|3c03]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">escU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">escU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bzx RCSB], [http://www.ebi.ac.uk/pdbsum/3bzx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bzx RCSB], [http://www.ebi.ac.uk/pdbsum/3bzx PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Deng, W.]]	+	[[Category: Deng, W]]
-	[[Category: Felise, H B.]]	+	[[Category: Felise, H B]]
-	[[Category: Finlay, B B.]]	+	[[Category: Finlay, B B]]
-	[[Category: Miller, S I.]]	+	[[Category: Miller, S I]]
-	[[Category: Nguyen, H V.]]	+	[[Category: Nguyen, H V]]
-	[[Category: Strynadka, N C.J.]]	+	[[Category: Strynadka, N C.J]]
-	[[Category: Vuckovic, M.]]	+	[[Category: Vuckovic, M]]
-	[[Category: Zarivach, R.]]	+	[[Category: Zarivach, R]]
	[[Category: Auto cleavage protein]]		[[Category: Auto cleavage protein]]
	[[Category: Intein]]		[[Category: Intein]]

OCA at 21:26, 2 October 2014

OCA — Thu, 02 Oct 2014 21:26:28 GMT

←Older revision		Revision as of 21:26, 2 October 2014
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-	[[~~Image:~~3bzx.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of the mutated H265A EscU C-terminal domain==
		+	<StructureSection load='3bzx' size='340' side='right' caption='[[3bzx]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3bzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BZX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bzo\|3bzo]], [[3bzl\|3bzl]], [[3bzp\|3bzp]], [[3bzr\|3bzr]], [[3bzs\|3bzs]], [[3bzt\|3bzt]], [[3bzv\|3bzv]], [[3bzy\|3bzy]], [[3bzz\|3bzz]], [[3c00\|3c00]], [[3c01\|3c01]], [[3c03\|3c03]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">escU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bzx RCSB], [http://www.ebi.ac.uk/pdbsum/3bzx PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/3bzx_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During infection by Gram-negative pathogenic bacteria, the type III secretion system (T3SS) is assembled to allow for the direct transmission of bacterial virulence effectors into the host cell. The T3SS system is characterized by a series of prominent multi-component rings in the inner and outer bacterial membranes, as well as a translocation pore in the host cell membrane. These are all connected by a series of polymerized tubes that act as the direct conduit for the T3SS proteins to pass through to the host cell. During assembly of the T3SS, as well as the evolutionarily related flagellar apparatus, a post-translational cleavage event within the inner membrane proteins EscU/FlhB is required to promote a secretion-competent state. These proteins have long been proposed to act as a part of a molecular switch, which would regulate the appropriate chronological secretion of the various T3SS apparatus components during assembly and subsequently the transported virulence effectors. Here we show that a surface type II beta-turn in the Escherichia coli protein EscU undergoes auto-cleavage by a mechanism involving cyclization of a strictly conserved asparagine residue. Structural and in vivo analysis of point and deletion mutations illustrates the subtle conformational effects of auto-cleavage in modulating the molecular features of a highly conserved surface region of EscU, a potential point of interaction with other T3SS components at the inner membrane. In addition, this work provides new structural insight into the distinct conformational requirements for a large class of self-cleaving reactions involving asparagine cyclization.

-	~~{{STRUCTURE_3bzx\| PDB=3bzx \| SCENE= }}~~	+	Structural analysis of the essential self-cleaving type III secretion proteins EscU and SpaS.,Zarivach R, Deng W, Vuckovic M, Felise HB, Nguyen HV, Miller SI, Finlay BB, Strynadka NC Nature. 2008 May 1;453(7191):124-7. PMID:18451864<ref>PMID:18451864</ref>

-	~~===Crystal structure~~ of the ~~mutated H265A EscU C-terminal domain===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18451864}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3bzx]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018451864<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Deng, W.]]		[[Category: Deng, W.]]

OCA: Protected "3bzx" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 21:01:03 GMT

Protected "3bzx" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:01, 7 January 2013

OCA at 21:01, 7 January 2013

OCA — Mon, 07 Jan 2013 21:01:01 GMT

←Older revision		Revision as of 21:01, 7 January 2013
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-	{{Seed}}
	[[Image:3bzx.png\|left\|200px]]		[[Image:3bzx.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3bzx", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
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	{{STRUCTURE_3bzx\| PDB=3bzx \| SCENE= }}		{{STRUCTURE_3bzx\| PDB=3bzx \| SCENE= }}

	===Crystal structure of the mutated H265A EscU C-terminal domain===		===Crystal structure of the mutated H265A EscU C-terminal domain===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18451864}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18451864 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_18451864}}		{{ABSTRACT_PUBMED_18451864}}

	==About this Structure==		==About this Structure==
-	~~3BZX~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA].	+	[[3bzx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18451864~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018451864</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Deng, W.]]		[[Category: Deng, W.]]
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	[[Category: Protein transport]]		[[Category: Protein transport]]
	[[Category: T3ss]]		[[Category: T3ss]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 22 11:22:14 2009''

OCA at 09:22, 22 March 2009

OCA — Sun, 22 Mar 2009 09:22:14 GMT

←Older revision		Revision as of 09:22, 22 March 2009
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	===Crystal structure of the mutated H265A EscU C-terminal domain===		===Crystal structure of the mutated H265A EscU C-terminal domain===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18451864}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18451864 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18451864}}

	==About this Structure==		==About this Structure==
	3BZX is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA].		3BZX is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:18451864</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Deng, W.]]		[[Category: Deng, W.]]
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	[[Category: T3ss]]		[[Category: T3ss]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Feb 18 00~~:09:15 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 22 11:22:14 2009''

OCA at 22:09, 17 February 2009

OCA — Tue, 17 Feb 2009 22:09:16 GMT

←Older revision		Revision as of 22:09, 17 February 2009
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-	[[Image:3bzx.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3bzx.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_3bzx\| PDB=3bzx \| SCENE= }}		{{STRUCTURE_3bzx\| PDB=3bzx \| SCENE= }}

-	~~'''~~Crystal structure of the mutated H265A EscU C-terminal domain~~'''~~	+	===Crystal structure of the mutated H265A EscU C-terminal domain===


	==About this Structure==		==About this Structure==
-	3BZX is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA].	+	3BZX is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZX OCA].
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Protein complex]]
	[[Category: Deng, W.]]		[[Category: Deng, W.]]
	[[Category: Felise, H B.]]		[[Category: Felise, H B.]]
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	[[Category: Protein transport]]		[[Category: Protein transport]]
	[[Category: T3ss]]		[[Category: T3ss]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~May 7 08~~:57:~~56 2008~~''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:09:15 2009''

OCA at 05:57, 7 May 2008

OCA — Wed, 07 May 2008 05:57:58 GMT

←Older revision		Revision as of 05:57, 7 May 2008
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	[[Category: Protein complex]]		[[Category: Protein complex]]
	[[Category: Deng, W.]]		[[Category: Deng, W.]]
		+	[[Category: Felise, H B.]]
	[[Category: Finlay, B B.]]		[[Category: Finlay, B B.]]
-	[[Category: ~~Hai~~, ~~V N~~.]]	+	[[Category: Miller, S I.]]
-	[[Category: ~~Heather~~, ~~B F.]]~~	+	[[Category: Nguyen, H V.]]
-	~~[[Category: Samuel, I M~~.]]	+
	[[Category: Strynadka, N C.J.]]		[[Category: Strynadka, N C.J.]]
	[[Category: Vuckovic, M.]]		[[Category: Vuckovic, M.]]
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	[[Category: Protein transport]]		[[Category: Protein transport]]
	[[Category: T3ss]]		[[Category: T3ss]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Apr 24 09:50~~:57 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 7 08:57:56 2008''