3c9u - Revision history

OCA at 07:48, 27 January 2022

OCA — Thu, 27 Jan 2022 07:48:22 GMT

←Older revision		Revision as of 07:48, 27 January 2022
Line 1:		Line 1:

	==AaThiL complexed with ADP and TPP==		==AaThiL complexed with ADP and TPP==
-	<StructureSection load='3c9u' size='340' side='right' caption='[[3c9u]], [[Resolution\|resolution]] 1.48Å' scene=''>	+	<StructureSection load='3c9u' size='340' side='right'caption='[[3c9u]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3c9u]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C9U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3C9U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3c9u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C9U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3c9r\|3c9r]], [[3c9s\|3c9s]], [[3c9t\|3c9t]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3c9r\|3c9r]], [[3c9s\|3c9s]], [[3c9t\|3c9t]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiL ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Thiamine-phosphate_kinase Thiamine-phosphate kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.16 2.7.4.16] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thiamine-phosphate_kinase Thiamine-phosphate kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.16 2.7.4.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3c9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c9u OCA], [~~http~~://pdbe.org/3c9u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3c9u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3c9u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3c9u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c9u OCA], [https://pdbe.org/3c9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c9u RCSB], [https://www.ebi.ac.uk/pdbsum/3c9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c9u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/THIL_AQUAE THIL_AQUAE]] Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.<ref>PMID:18311927</ref>	+	[[https://www.uniprot.org/uniprot/THIL_AQUAE THIL_AQUAE]] Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.<ref>PMID:18311927</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c9/3c9u_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c9/3c9u_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Aquifex aeolicus huber and stetter 2001]]		[[Category: Aquifex aeolicus huber and stetter 2001]]
		+	[[Category: Large Structures]]
	[[Category: Thiamine-phosphate kinase]]		[[Category: Thiamine-phosphate kinase]]
	[[Category: Begley, T P]]		[[Category: Begley, T P]]

OCA at 08:21, 25 October 2017

OCA — Wed, 25 Oct 2017 08:21:30 GMT

←Older revision		Revision as of 08:21, 25 October 2017
Line 1:		Line 1:
		+
	==AaThiL complexed with ADP and TPP==		==AaThiL complexed with ADP and TPP==
	<StructureSection load='3c9u' size='340' side='right' caption='[[3c9u]], [[Resolution\|resolution]] 1.48Å' scene=''>		<StructureSection load='3c9u' size='340' side='right' caption='[[3c9u]], [[Resolution\|resolution]] 1.48Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_kinase Thiamine-phosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.16 2.7.4.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_kinase Thiamine-phosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.16 2.7.4.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c9u OCA], [http://pdbe.org/3c9u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c9u RCSB], [http://www.ebi.ac.uk/pdbsum/3c9u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c9u OCA], [http://pdbe.org/3c9u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c9u RCSB], [http://www.ebi.ac.uk/pdbsum/3c9u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c9u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 05:55, 8 February 2016

OCA — Mon, 08 Feb 2016 05:55:43 GMT

←Older revision		Revision as of 05:55, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='3c9u' size='340' side='right' caption='[[3c9u]], [[Resolution\|resolution]] 1.48Å' scene=''>		<StructureSection load='3c9u' size='340' side='right' caption='[[3c9u]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3c9u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aquifex_aeolicus Aquifex~~ aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C9U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C9U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3c9u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C9U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C9U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3c9r\|3c9r]], [[3c9s\|3c9s]], [[3c9t\|3c9t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3c9r\|3c9r]], [[3c9s\|3c9s]], [[3c9t\|3c9t]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_kinase Thiamine-phosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.16 2.7.4.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_kinase Thiamine-phosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.16 2.7.4.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c9u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c9u RCSB], [http://www.ebi.ac.uk/pdbsum/3c9u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c9u OCA], [http://pdbe.org/3c9u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c9u RCSB], [http://www.ebi.ac.uk/pdbsum/3c9u PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/THIL_AQUAE THIL_AQUAE]] Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.<ref>PMID:18311927</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c9u ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3c9u" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aquifex aeolicus]]	+	[[Category: Aquifex aeolicus huber and stetter 2001]]
	[[Category: Thiamine-phosphate kinase]]		[[Category: Thiamine-phosphate kinase]]
	[[Category: Begley, T P]]		[[Category: Begley, T P]]

OCA at 13:41, 18 December 2014

OCA — Thu, 18 Dec 2014 13:41:15 GMT

←Older revision		Revision as of 13:41, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3c9u\| PDB~~=3c9u \| ~~SCENE~~= }}	+	==AaThiL complexed with ADP and TPP==
-	===~~AaThiL complexed~~ with ADP ~~and~~ TPP===	+	<StructureSection load='3c9u' size='340' side='right' caption='[[3c9u]], [[Resolution\|resolution]] 1.48Å' scene=''>
-	~~{{ABSTRACT_PUBMED_18311927}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3c9u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C9U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C9U FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3c9r\|3c9r]], [[3c9s\|3c9s]], [[3c9t\|3c9t]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_kinase Thiamine-phosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.16 2.7.4.16] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c9u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c9u RCSB], [http://www.ebi.ac.uk/pdbsum/3c9u PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c9/3c9u_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Thiamin monophosphate kinase (ThiL) catalyzes the ATP-dependent phosphorylation of thiamin monophosphate (TMP) to form thiamin pyrophosphate (TPP), the active form of vitamin B 1. ThiL is a member of a small ATP binding superfamily that also includes the purine biosynthetic enzymes, PurM and PurL, NiFe hydrogenase maturation protein, HypE, and selenophosphate synthase, SelD. The latter four enzymes are believed to utilize phosphorylated intermediates during catalysis. To understand the mechanism of ThiL and its relationship to the other superfamily members, we determined the structure of Aquifex aeolicus ThiL (AaThiL) with nonhydrolyzable AMP-PCP and TMP, and also with the products of the reaction, ADP and TPP. The results suggest that AaThiL utilizes a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate. The structure of ThiL is compared to those of PurM, PurL, and HypE, and the ATP binding site is compared to that of PurL, for which nucleotide complexes are available.

-	~~==About this Structure==~~	+	Structural studies of thiamin monophosphate kinase in complex with substrates and products(,).,McCulloch KM, Kinsland C, Begley TP, Ealick SE Biochemistry. 2008 Mar 25;47(12):3810-21. Epub 2008 Mar 1. PMID:18311927<ref>PMID:18311927</ref>
-	~~[[3c9u]] is a 2 chain structure~~ with ~~sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3C9U OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:018311927</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Aquifex aeolicus]]		[[Category: Aquifex aeolicus]]
	[[Category: Thiamine-phosphate kinase]]		[[Category: Thiamine-phosphate kinase]]
-	[[Category: Begley, T P.]]	+	[[Category: Begley, T P]]
-	[[Category: Ealick, S E.]]	+	[[Category: Ealick, S E]]
-	[[Category: Kinsland, C.]]	+	[[Category: Kinsland, C]]
-	[[Category: McCulloch, K M.]]	+	[[Category: McCulloch, K M]]
	[[Category: Alpha-beta structure]]		[[Category: Alpha-beta structure]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Kinase]]		[[Category: Kinase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 12:28, 3 July 2013

OCA — Wed, 03 Jul 2013 12:28:42 GMT

←Older revision		Revision as of 12:28, 3 July 2013
Line 1:		Line 1:
-	[[Image:3c9u.png\|left\|200px]]
-
	{{STRUCTURE_3c9u\| PDB=3c9u \| SCENE= }}		{{STRUCTURE_3c9u\| PDB=3c9u \| SCENE= }}
-
	===AaThiL complexed with ADP and TPP===		===AaThiL complexed with ADP and TPP===
-
	{{ABSTRACT_PUBMED_18311927}}		{{ABSTRACT_PUBMED_18311927}}

Line 11:		Line 7:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:018311927</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018311927</ref><references group="xtra"/><references/>
	[[Category: Aquifex aeolicus]]		[[Category: Aquifex aeolicus]]
	[[Category: Thiamine-phosphate kinase]]		[[Category: Thiamine-phosphate kinase]]

OCA: Protected "3c9u" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 22:06:05 GMT

Protected "3c9u" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:06, 7 January 2013

OCA at 22:06, 7 January 2013

OCA — Mon, 07 Jan 2013 22:06:03 GMT

←Older revision		Revision as of 22:06, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3c9u.png\|left\|200px]]		[[Image:3c9u.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3c9u", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3c9u\| PDB=3c9u \| SCENE= }}		{{STRUCTURE_3c9u\| PDB=3c9u \| SCENE= }}

	===AaThiL complexed with ADP and TPP===		===AaThiL complexed with ADP and TPP===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18311927}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18311927 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18311927}}		{{ABSTRACT_PUBMED_18311927}}

	==About this Structure==		==About this Structure==
-	~~3C9U~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C9U OCA].	+	[[3c9u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C9U OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18311927~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018311927</ref><references group="xtra"/>
	[[Category: Aquifex aeolicus]]		[[Category: Aquifex aeolicus]]
	[[Category: Thiamine-phosphate kinase]]		[[Category: Thiamine-phosphate kinase]]
Line 34:		Line 22:
	[[Category: Kinase]]		[[Category: Kinase]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 19:37:06 2009''

OCA at 17:37, 16 February 2009

OCA — Mon, 16 Feb 2009 17:37:08 GMT

←Older revision		Revision as of 17:37, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3C9U is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C9U OCA].	+	3C9U is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C9U OCA].

	==Reference==		==Reference==
-	Structural studies of thiamin monophosphate kinase in complex with substrates and products(,)., McCulloch KM, Kinsland C, Begley TP, Ealick SE, Biochemistry. 2008 Mar 25;47(12):3810-21. Epub 2008 Mar 1. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18311927 18311927]~~	+	<ref group="xtra">PMID:18311927</ref><references group="xtra"/>
	[[Category: Aquifex aeolicus]]		[[Category: Aquifex aeolicus]]
-	[[Category: Single protein]]
	[[Category: Thiamine-phosphate kinase]]		[[Category: Thiamine-phosphate kinase]]
	[[Category: Begley, T P.]]		[[Category: Begley, T P.]]
Line 36:		Line 35:
	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 23~~:41:~~45 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 19:37:06 2009''

OCA at 20:41, 27 July 2008

OCA — Sun, 27 Jul 2008 20:41:46 GMT

←Older revision		Revision as of 20:41, 27 July 2008
Line 1:		Line 1:
-	[[Image:3c9u.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3c9u.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_3c9u\| PDB=3c9u \| SCENE= }}		{{STRUCTURE_3c9u\| PDB=3c9u \| SCENE= }}

-	~~'''~~AaThiL complexed with ADP and TPP~~'''~~	+	===AaThiL complexed with ADP and TPP===


-	~~==Overview==~~	+	<!--
-	~~Thiamin monophosphate kinase (ThiL) catalyzes the ATP-dependent phosphorylation of thiamin monophosphate (TMP) to form thiamin pyrophosphate (TPP)~~, ~~the active form of vitamin B 1. ThiL is a member of a small ATP binding superfamily that also includes the purine biosynthetic enzymes~~, PurM and PurL, NiFe hydrogenase maturation protein, HypE, and selenophosphate synthase, SelD. The latter four enzymes are believed to utilize phosphorylated intermediates during catalysis. To understand the ~~mechanism of ThiL and its relationship~~ to the ~~other superfamily members, we determined the structure of Aquifex aeolicus ThiL~~ (~~AaThiL~~) ~~with nonhydrolyzable AMP-PCP and TMP~~, ~~and also with~~ the ~~products of the reaction, ADP and TPP~~. ~~The results suggest that AaThiL utilizes a direct, inline transfer of the gamma~~-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate. The structure of ThiL is compared to those of PurM, PurL, and HypE, and the ATP binding site is compared to that of PurL, for which nucleotide complexes are available.	+	The line below this paragraph, {{ABSTRACT_PUBMED_18311927}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18311927 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18311927}}

	==About this Structure==		==About this Structure==
Line 31:		Line 35:
	[[Category: Kinase]]		[[Category: Kinase]]
	[[Category: Transferase]]		[[Category: Transferase]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun ~~May 4 21~~:30:35 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:41:45 2008''

OCA at 18:30, 4 May 2008

OCA — Sun, 04 May 2008 18:30:36 GMT

←Older revision		Revision as of 18:30, 4 May 2008
Line 1:		Line 1:
	[[Image:3c9u.jpg\|left\|200px]]		[[Image:3c9u.jpg\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 3c9u \|SIZE=350\|CAPTION=~~ <~~scene name='initialview01'>3c9u</scene>, resolution 1.480Å~~	+	The line below this paragraph, containing "STRUCTURE_3c9u", creates the "Structure Box" on the page.
-	\|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Residue+A+309'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Residue+A+310'>AC2</scene>, <scene name='pdbsite=AC3:Mg+Binding+Site+For+Residue+A+311'>AC3</scene>, <scene name='pdbsite=AC4:Mg+Binding+Site+For+Residue+A+312'>AC4</scene>, <scene name='pdbsite=AC5:Mg+Binding+Site+For+Residue+A+313'>AC5</scene>, <scene name='pdbsite=AC6:Mg+Binding+Site+For+Residue+B+309'>AC6</scene>, <scene name='pdbsite=AC7:Mg+Binding+Site+For+Residue+B+310'>AC7</scene>, <scene name='pdbsite=AC8:Mg+Binding+Site+For+Residue+B+311'>AC8</scene>, <scene name='pdbsite=AC9:Mg+Binding+Site+For+Residue+B+312'>AC9</scene>, <scene name='pdbsite=BC1:Mg+Binding+Site+For+Residue+A+314'>BC1</scene>, <scene name='pdbsite=BC2:Adp+Binding+Site+For+Residue+B+307'>BC2</scene>, <scene name='pdbsite=BC3:Tpp+Binding+Site+For+Residue+A+308'>BC3</scene>, <scene name='pdbsite=BC4:Adp+Binding+Site+For+Residue+A+307'>BC4</scene> and <scene name='pdbsite=BC5:Tpp+Binding+Site+For+Residue+B+308'>BC5</scene>	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <scene name='pdbligand=ADP:ADENOSINE~~-~~5'~~-~~DIPHOSPHATE'>ADP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene>~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY= <span class='plainlinks'>[http://en~~.~~wikipedia.org/wiki/Thiamine-phosphate_kinase Thiamine-phosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.16 2.7.4.16] </span>~~	+	or leave the SCENE parameter empty for the default display.
-	~~\|GENE= thiL~~ (~~[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]~~)	+	-->
-	~~\|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd02194 ThiL_N]~~, ~~[http://www~~.~~ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0611 ThiL]</span~~>	+	{{STRUCTURE_3c9u\| PDB=3c9u \| SCENE= }}
-	\|~~RELATEDENTRY~~=~~[[3c9r~~\|~~3C9R]], [[3c9s\|3C9S]], [[3c9t\|3C9T]]~~	+
-	~~\|RESOURCES~~=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c9u OCA], [http://www.ebi.ac.uk/pdbsum/3c9u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3c9u RCSB]</span>	+
-	}}	+

	'''AaThiL complexed with ADP and TPP'''		'''AaThiL complexed with ADP and TPP'''
Line 30:		Line 27:
	[[Category: Kinsland, C.]]		[[Category: Kinsland, C.]]
	[[Category: McCulloch, K M.]]		[[Category: McCulloch, K M.]]
-	[[Category: ~~alpha~~-beta structure]]	+	[[Category: Alpha-beta structure]]
-	[[Category: ~~beta~~ barrel]]	+	[[Category: Beta barrel]]
-	[[Category: ~~kinase~~]]	+	[[Category: Kinase]]
-	[[Category: ~~transferase~~]]	+	[[Category: Transferase]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 21:30:35 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 05~~:31:22 2008''	+