3cg3 - Revision history

OCA at 09:35, 21 February 2024

OCA — Wed, 21 Feb 2024 09:35:16 GMT

←Older revision		Revision as of 09:35, 21 February 2024
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	<StructureSection load='3cg3' size='340' side='right'caption='[[3cg3]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3cg3' size='340' side='right'caption='[[3cg3]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cg3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~'pyrococcus_shinkaii' 'pyrococcus shinkaii'~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CG3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cg3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CG3 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[2onr\|2onr]], [[3cfx\|3cfx]], [[3cg1\|3cg1]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">modA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 'Pyrococcus shinkaii'])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cg3 OCA], [https://pdbe.org/3cg3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cg3 RCSB], [https://www.ebi.ac.uk/pdbsum/3cg3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cg3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cg3 OCA], [https://pdbe.org/3cg3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cg3 RCSB], [https://www.ebi.ac.uk/pdbsum/3cg3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cg3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/WTPA_PYRHO WTPA_PYRHO]] Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Binds tungstate and molybdate (By similarity).	+	[https://www.uniprot.org/uniprot/WTPA_PYRHO WTPA_PYRHO] Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Binds tungstate and molybdate (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cg3 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cg3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Bacteria and archaea import molybdenum and tungsten from the environment in the form of the oxyanions molybdate (MoO(4) (2-)) and tungstate (WO(4) (2-)). These substrates are captured by an external, high-affinity binding protein, and delivered to ATP binding cassette transporters, which move them across the cell membrane. We have recently reported a crystal structure of the molybdate/tungstate binding protein ModA/WtpA from Archaeoglobus fulgidus, which revealed an octahedrally coordinated central metal atom. By contrast, the previously determined structures of three bacterial homologs showed tetracoordinate molybdenum and tungsten atoms in their binding pockets. Until then, coordination numbers above four had only been found for molybdenum/tungsten in metalloenzymes where these metal atoms are part of the catalytic cofactors and coordinated by mostly non-oxygen ligands. We now report a high-resolution structure of A. fulgidus ModA/WtpA, as well as crystal structures of four additional homologs, all bound to tungstate. These crystal structures match X-ray absorption spectroscopy measurements from soluble, tungstate-bound protein, and reveal the details of the distorted octahedral coordination. Our results demonstrate that the distorted octahedral geometry is not an exclusive feature of the A. fulgidus protein, and suggest distinct binding modes of the binding proteins from archaea and bacteria.
-
-	Distorted octahedral coordination of tungstate in a subfamily of specific binding proteins.,Hollenstein K, Comellas-Bigler M, Bevers LE, Feiters MC, Meyer-Klaucke W, Hagedoorn PL, Locher KP J Biol Inorg Chem. 2009 Feb 21. PMID:19234723<ref>PMID:19234723</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3cg3" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[ABC transporter 3D structures\|ABC transporter 3D structures]]		*[[ABC transporter 3D structures\|ABC transporter 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pyrococcus shinkaii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Comellas-Bigler, M~~]]	+	[[Category: Pyrococcus horikoshii]]
-	[[Category: ~~Hollenstein, K~~]]	+	[[Category: Comellas-Bigler M]]
-	[[Category: ~~Locher,~~ K P]]	+	[[Category: Hollenstein K]]
-	[[Category: ~~Abc transporter]]~~	+	[[Category: Locher KP]]
-	~~[[Category: Binding protein]]~~	+
-	~~[[Category: Ligand]]~~	+
-	~~[[Category: Molybdate]]~~	+
-	~~[[Category: Transport protein]]~~	+
-	~~[[Category: Tungstate]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 07:54, 27 January 2022

OCA — Thu, 27 Jan 2022 07:54:44 GMT

←Older revision		Revision as of 07:54, 27 January 2022
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	==Crystal structure of P. horikoshii periplasmic binding protein ModA/WtpA with bound tungstate==		==Crystal structure of P. horikoshii periplasmic binding protein ModA/WtpA with bound tungstate==
-	<StructureSection load='3cg3' size='340' side='right' caption='[[3cg3]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='3cg3' size='340' side='right'caption='[[3cg3]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cg3]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CG3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cg3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CG3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2onr\|2onr]], [[3cfx\|3cfx]], [[3cg1\|3cg1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2onr\|2onr]], [[3cfx\|3cfx]], [[3cg1\|3cg1]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">modA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 'Pyrococcus shinkaii'])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">modA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 'Pyrococcus shinkaii'])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3cg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cg3 OCA], [~~http~~://pdbe.org/3cg3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3cg3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3cg3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3cg3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cg3 OCA], [https://pdbe.org/3cg3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cg3 RCSB], [https://www.ebi.ac.uk/pdbsum/3cg3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cg3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/WTPA_PYRHO WTPA_PYRHO]] Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Binds tungstate and molybdate (By similarity).	+	[[https://www.uniprot.org/uniprot/WTPA_PYRHO WTPA_PYRHO]] Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Binds tungstate and molybdate (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[ABC transporter\|ABC transporter]]	+	*[[ABC transporter 3D structures\|ABC transporter 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Pyrococcus shinkaii]]		[[Category: Pyrococcus shinkaii]]
		+	[[Category: Large Structures]]
	[[Category: Comellas-Bigler, M]]		[[Category: Comellas-Bigler, M]]
	[[Category: Hollenstein, K]]		[[Category: Hollenstein, K]]

OCA at 14:01, 7 November 2018

OCA — Wed, 07 Nov 2018 14:01:57 GMT

←Older revision		Revision as of 14:01, 7 November 2018
Line 1:		Line 1:
		+
	==Crystal structure of P. horikoshii periplasmic binding protein ModA/WtpA with bound tungstate==		==Crystal structure of P. horikoshii periplasmic binding protein ModA/WtpA with bound tungstate==
	<StructureSection load='3cg3' size='340' side='right' caption='[[3cg3]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3cg3' size='340' side='right' caption='[[3cg3]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2onr\|2onr]], [[3cfx\|3cfx]], [[3cg1\|3cg1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2onr\|2onr]], [[3cfx\|3cfx]], [[3cg1\|3cg1]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">modA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 'Pyrococcus shinkaii'])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">modA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 'Pyrococcus shinkaii'])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cg3 OCA], [http://pdbe.org/3cg3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cg3 RCSB], [http://www.ebi.ac.uk/pdbsum/3cg3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cg3 OCA], [http://pdbe.org/3cg3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cg3 RCSB], [http://www.ebi.ac.uk/pdbsum/3cg3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cg3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/3cg3_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/3cg3_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 01:53, 9 February 2016

OCA — Tue, 09 Feb 2016 01:53:12 GMT

←Older revision		Revision as of 01:53, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3cg3' size='340' side='right' caption='[[3cg3]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3cg3' size='340' side='right' caption='[[3cg3]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cg3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pyrococcus_horikoshii Pyrococcus horikoshii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CG3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cg3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CG3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2onr\|2onr]], [[3cfx\|3cfx]], [[3cg1\|3cg1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2onr\|2onr]], [[3cfx\|3cfx]], [[3cg1\|3cg1]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">modA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus ~~horikoshii~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">modA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 'Pyrococcus shinkaii'])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cg3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cg3 RCSB], [http://www.ebi.ac.uk/pdbsum/3cg3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cg3 OCA], [http://pdbe.org/3cg3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cg3 RCSB], [http://www.ebi.ac.uk/pdbsum/3cg3 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/WTPA_PYRHO WTPA_PYRHO]] Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Binds tungstate and molybdate (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cg3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3cg3" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pyrococcus ~~horikoshii~~]]	+	[[Category: Pyrococcus shinkaii]]
	[[Category: Comellas-Bigler, M]]		[[Category: Comellas-Bigler, M]]
	[[Category: Hollenstein, K]]		[[Category: Hollenstein, K]]

OCA at 09:49, 20 January 2015

OCA — Tue, 20 Jan 2015 09:49:11 GMT

←Older revision		Revision as of 09:49, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3cg3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CG3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3cg3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CG3 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2onr\|2onr]], [[3cfx\|3cfx]], [[3cg1\|3cg1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2onr\|2onr]], [[3cfx\|3cfx]], [[3cg1\|3cg1]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">modA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">modA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cg3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cg3 RCSB], [http://www.ebi.ac.uk/pdbsum/3cg3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cg3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cg3 RCSB], [http://www.ebi.ac.uk/pdbsum/3cg3 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
-	[[Category: Comellas-Bigler, M.]]	+	[[Category: Comellas-Bigler, M]]
-	[[Category: Hollenstein, K.]]	+	[[Category: Hollenstein, K]]
-	[[Category: Locher, K P.]]	+	[[Category: Locher, K P]]
	[[Category: Abc transporter]]		[[Category: Abc transporter]]
	[[Category: Binding protein]]		[[Category: Binding protein]]

OCA at 08:14, 29 September 2014

OCA — Mon, 29 Sep 2014 08:14:12 GMT

←Older revision		Revision as of 08:14, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3cg3.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of P. horikoshii periplasmic binding protein ModA/WtpA with bound tungstate==
		+	<StructureSection load='3cg3' size='340' side='right' caption='[[3cg3]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3cg3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CG3 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2onr\|2onr]], [[3cfx\|3cfx]], [[3cg1\|3cg1]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">modA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cg3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cg3 RCSB], [http://www.ebi.ac.uk/pdbsum/3cg3 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/3cg3_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria and archaea import molybdenum and tungsten from the environment in the form of the oxyanions molybdate (MoO(4) (2-)) and tungstate (WO(4) (2-)). These substrates are captured by an external, high-affinity binding protein, and delivered to ATP binding cassette transporters, which move them across the cell membrane. We have recently reported a crystal structure of the molybdate/tungstate binding protein ModA/WtpA from Archaeoglobus fulgidus, which revealed an octahedrally coordinated central metal atom. By contrast, the previously determined structures of three bacterial homologs showed tetracoordinate molybdenum and tungsten atoms in their binding pockets. Until then, coordination numbers above four had only been found for molybdenum/tungsten in metalloenzymes where these metal atoms are part of the catalytic cofactors and coordinated by mostly non-oxygen ligands. We now report a high-resolution structure of A. fulgidus ModA/WtpA, as well as crystal structures of four additional homologs, all bound to tungstate. These crystal structures match X-ray absorption spectroscopy measurements from soluble, tungstate-bound protein, and reveal the details of the distorted octahedral coordination. Our results demonstrate that the distorted octahedral geometry is not an exclusive feature of the A. fulgidus protein, and suggest distinct binding modes of the binding proteins from archaea and bacteria.

-	~~{{STRUCTURE_3cg3\| PDB=3cg3 \| SCENE= }}~~	+	Distorted octahedral coordination of tungstate in a subfamily of specific binding proteins.,Hollenstein K, Comellas-Bigler M, Bevers LE, Feiters MC, Meyer-Klaucke W, Hagedoorn PL, Locher KP J Biol Inorg Chem. 2009 Feb 21. PMID:19234723<ref>PMID:19234723</ref>

-	~~===Crystal structure of P. horikoshii periplasmic binding protein ModA~~/~~WtpA with bound tungstate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19234723}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3cg3]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA].~~	+

	==See Also==		==See Also==
	*[[ABC transporter\|ABC transporter]]		*[[ABC transporter\|ABC transporter]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019234723<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
	[[Category: Comellas-Bigler, M.]]		[[Category: Comellas-Bigler, M.]]

OCA at 12:14, 20 October 2012

OCA — Sat, 20 Oct 2012 12:14:18 GMT

←Older revision		Revision as of 12:14, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3cg3]] is a 1 chain structure ~~of [[ABC transporter]]~~ with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA].	+	[[3cg3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA].

	==See Also==		==See Also==

OCA: Protected "3cg3" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 08:02:54 GMT

Protected "3cg3" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:02, 26 July 2012

OCA at 08:02, 26 July 2012

OCA — Thu, 26 Jul 2012 08:02:53 GMT

←Older revision		Revision as of 08:02, 26 July 2012
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-	{{Seed}}
	[[Image:3cg3.png\|left\|200px]]		[[Image:3cg3.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3cg3", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_3cg3\| PDB=3cg3 \| SCENE= }}		{{STRUCTURE_3cg3\| PDB=3cg3 \| SCENE= }}

	===Crystal structure of P. horikoshii periplasmic binding protein ModA/WtpA with bound tungstate===		===Crystal structure of P. horikoshii periplasmic binding protein ModA/WtpA with bound tungstate===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19234723}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19234723 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_19234723}}		{{ABSTRACT_PUBMED_19234723}}

	==About this Structure==		==About this Structure==
-	~~3CG3~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA].	+	[[3cg3]] is a 1 chain structure of [[ABC transporter]] with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CG3 OCA].
		+
		+	==See Also==
		+	*[[ABC transporter\|ABC transporter]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19234723~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019234723</ref><references group="xtra"/>
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
	[[Category: Comellas-Bigler, M.]]		[[Category: Comellas-Bigler, M.]]
Line 30:		Line 21:
	[[Category: Abc transporter]]		[[Category: Abc transporter]]
	[[Category: Binding protein]]		[[Category: Binding protein]]
-	[[Category: Crystal structure]]
	[[Category: Ligand]]		[[Category: Ligand]]
	[[Category: Molybdate]]		[[Category: Molybdate]]
Line 36:		Line 26:
	[[Category: Tungstate]]		[[Category: Tungstate]]
	[[Category: Unknown function]]		[[Category: Unknown function]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 22 20:27:18 2009''

OCA at 17:21, 22 July 2009

OCA — Wed, 22 Jul 2009 17:21:44 GMT

←Older revision		Revision as of 17:21, 22 July 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3cg3.~~jpg~~\|left\|200px]]	+	[[Image:3cg3.png\|left\|200px]]

	<!--		<!--
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	[[Category: Unknown function]]		[[Category: Unknown function]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar 11 11:~~22:54 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 22 20:27:18 2009''