3cgy - Revision history

OCA at 12:28, 30 August 2023

OCA — Wed, 30 Aug 2023 12:28:24 GMT

←Older revision		Revision as of 12:28, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='3cgy' size='340' side='right'caption='[[3cgy]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3cgy' size='340' side='right'caption='[[3cgy]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cgy]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3CGY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cgy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CGY FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=RDC:RADICICOL'>RDC</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[3cgz\|3cgz]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RDC:RADICICOL'>RDC</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cgy OCA], [https://pdbe.org/3cgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cgy RCSB], [https://www.ebi.ac.uk/pdbsum/3cgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cgy ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cgy OCA], [~~http~~://pdbe.org/3cgy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3cgy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3cgy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3cgy ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PHOQ_SALTY PHOQ_SALTY]] Member of the two-component regulatory system PhoP/PhoQ which regulates the expression of genes involved in virulence, adaptation to acidic and low Mg(2+) environments and resistance to host defense antimicrobial peptides. Essential for intramacrophage survival of S.typhimurium. In low periplasmic Mg(2+), PhoQ functions as a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+), acts as a protein phosphatase that dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Essential for transcription of spiC inside macrophages by controlling the expression of the two-component regulatory system SsrB/SpiR (SsrA) and Pir at transcriptional and post-transcriptional levels respectively. Promotes expression of the two-component regulatory system PmrA/PmrB via activation of pmrD gene. Is required to attenuate bacterial growth within fibroblast cells and to enhance bacterial resistance to bile in intestinal cells. Negatively regulates prgH, which is required for invasion of epithelial cells. Involved in acid tolerance.<ref>PMID:2544889</ref> <ref>PMID:9573193</ref> <ref>PMID:15910283</ref>	+	[https://www.uniprot.org/uniprot/PHOQ_SALTY PHOQ_SALTY] Member of the two-component regulatory system PhoP/PhoQ which regulates the expression of genes involved in virulence, adaptation to acidic and low Mg(2+) environments and resistance to host defense antimicrobial peptides. Essential for intramacrophage survival of S.typhimurium. In low periplasmic Mg(2+), PhoQ functions as a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+), acts as a protein phosphatase that dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Essential for transcription of spiC inside macrophages by controlling the expression of the two-component regulatory system SsrB/SpiR (SsrA) and Pir at transcriptional and post-transcriptional levels respectively. Promotes expression of the two-component regulatory system PmrA/PmrB via activation of pmrD gene. Is required to attenuate bacterial growth within fibroblast cells and to enhance bacterial resistance to bile in intestinal cells. Negatively regulates prgH, which is required for invasion of epithelial cells. Involved in acid tolerance.<ref>PMID:2544889</ref> <ref>PMID:9573193</ref> <ref>PMID:15910283</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Histidine kinase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Guarnieri~~, M T~~]]	+	[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-	[[Category: Shen, J]]	+	[[Category: Guarnieri MT]]
-	[[Category: Zhang, L]]	+	[[Category: Shen J]]
-	[[Category: Zhao, R]]	+	[[Category: Zhang L]]
-	~~[[Category: Alpha-beta sandwich]]~~	+	[[Category: Zhao R]]
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Bergerat fold]]~~	+
-	~~[[Category: Growth regulation]]~~	+
-	~~[[Category: Inner membrane]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Magnesium]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Transmembrane]]~~	+
-	~~[[Category: Two-component regulatory system]]~~	+
-	~~[[Category: Virulence~~]]	+

OCA at 10:27, 12 August 2020

OCA — Wed, 12 Aug 2020 10:27:06 GMT

←Older revision		Revision as of 10:27, 12 August 2020
Line 1:		Line 1:

	==Crystal Structure of Salmonella Sensor Kinase PhoQ catalytic domain in complex with radicicol==		==Crystal Structure of Salmonella Sensor Kinase PhoQ catalytic domain in complex with radicicol==
-	<StructureSection load='3cgy' size='340' side='right' caption='[[3cgy]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='3cgy' size='340' side='right'caption='[[3cgy]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cgy]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CGY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cgy]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3CGY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RDC:RADICICOL'>RDC</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RDC:RADICICOL'>RDC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3cgz\|3cgz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3cgz\|3cgz]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cgy OCA], [http://pdbe.org/3cgy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cgy RCSB], [http://www.ebi.ac.uk/pdbsum/3cgy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cgy ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cgy OCA], [http://pdbe.org/3cgy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cgy RCSB], [http://www.ebi.ac.uk/pdbsum/3cgy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cgy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
		+	[[Category: Large Structures]]
	[[Category: Guarnieri, M T]]		[[Category: Guarnieri, M T]]
	[[Category: Shen, J]]		[[Category: Shen, J]]

OCA at 13:44, 7 November 2018

OCA — Wed, 07 Nov 2018 13:44:14 GMT

←Older revision		Revision as of 13:44, 7 November 2018
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/3cgy_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/3cgy_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:24, 5 August 2016

OCA — Fri, 05 Aug 2016 20:24:59 GMT

←Older revision		Revision as of 20:24, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Salmonella Sensor Kinase PhoQ catalytic domain in complex with radicicol==		==Crystal Structure of Salmonella Sensor Kinase PhoQ catalytic domain in complex with radicicol==
	<StructureSection load='3cgy' size='340' side='right' caption='[[3cgy]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3cgy' size='340' side='right' caption='[[3cgy]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cgy]] is a 3 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CGY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cgy]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CGY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RDC:RADICICOL'>RDC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RDC:RADICICOL'>RDC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3cgz\|3cgz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3cgz\|3cgz]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phoQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 Salmonella enterica subsp. enterica serovar Typhimurium])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cgy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cgy RCSB], [http://www.ebi.ac.uk/pdbsum/3cgy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cgy OCA], [http://pdbe.org/3cgy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cgy RCSB], [http://www.ebi.ac.uk/pdbsum/3cgy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cgy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cgy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3cgy" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
-	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
	[[Category: Guarnieri, M T]]		[[Category: Guarnieri, M T]]
	[[Category: Shen, J]]		[[Category: Shen, J]]

OCA at 21:37, 25 December 2014

OCA — Thu, 25 Dec 2014 21:37:16 GMT

←Older revision		Revision as of 21:37, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3cgy]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CGY FirstGlance]. <br>		<table><tr><td colspan='2'>[[3cgy]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CGY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RDC:RADICICOL'>RDC</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RDC:RADICICOL'>RDC</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3cgz\|3cgz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3cgz\|3cgz]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phoQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 Salmonella enterica subsp. enterica serovar Typhimurium])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phoQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 Salmonella enterica subsp. enterica serovar Typhimurium])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cgy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cgy RCSB], [http://www.ebi.ac.uk/pdbsum/3cgy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cgy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cgy RCSB], [http://www.ebi.ac.uk/pdbsum/3cgy PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PHOQ_SALTY PHOQ_SALTY]] Member of the two-component regulatory system PhoP/PhoQ which regulates the expression of genes involved in virulence, adaptation to acidic and low Mg(2+) environments and resistance to host defense antimicrobial peptides. Essential for intramacrophage survival of S.typhimurium. In low periplasmic Mg(2+), PhoQ functions as a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+), acts as a protein phosphatase that dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Essential for transcription of spiC inside macrophages by controlling the expression of the two-component regulatory system SsrB/SpiR (SsrA) and Pir at transcriptional and post-transcriptional levels respectively. Promotes expression of the two-component regulatory system PmrA/PmrB via activation of pmrD gene. Is required to attenuate bacterial growth within fibroblast cells and to enhance bacterial resistance to bile in intestinal cells. Negatively regulates prgH, which is required for invasion of epithelial cells. Involved in acid tolerance.<ref>PMID:2544889</ref> <ref>PMID:9573193</ref> <ref>PMID:15910283</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
-	[[Category: Guarnieri, M T.]]	+	[[Category: Guarnieri, M T]]
-	[[Category: Shen, J.]]	+	[[Category: Shen, J]]
-	[[Category: Zhang, L.]]	+	[[Category: Zhang, L]]
-	[[Category: Zhao, R.]]	+	[[Category: Zhao, R]]
	[[Category: Alpha-beta sandwich]]		[[Category: Alpha-beta sandwich]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]

OCA at 07:52, 29 September 2014

OCA — Mon, 29 Sep 2014 07:52:35 GMT

←Older revision		Revision as of 07:52, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3cgy.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Salmonella Sensor Kinase PhoQ catalytic domain in complex with radicicol==
		+	<StructureSection load='3cgy' size='340' side='right' caption='[[3cgy]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3cgy]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CGY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RDC:RADICICOL'>RDC</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3cgz\|3cgz]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phoQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 Salmonella enterica subsp. enterica serovar Typhimurium])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cgy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cgy RCSB], [http://www.ebi.ac.uk/pdbsum/3cgy PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/3cgy_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sensor kinases in the bacterial two-component system share a unique ATP-binding Bergerat fold with the GHL (gyrase, Hsp90, and MutL) family of proteins. We demonstrated that selected GHL inhibitors bind to the catalytic domain of sensor kinase PhoQ (PhoQcat) using NMR chemical shift perturbation experiments. Using crystallographic approaches, we show that radicicol (an Hsp90 inhibitor) binds and interacts specifically with residues in the ATP-binding pocket of PhoQ. The interaction between radicicol and PhoQcat demonstrates significant similarities as well as differences compared to AMPPNP (a non-hydrolyzable ATP analog) bound to PhoQcat and radicicol bound to Hsp90. Our results suggest that GHL inhibitors may be useful lead compounds for developing sensor kinase inhibitors.

-	~~{{STRUCTURE_3cgy\| PDB=3cgy \| SCENE= }}~~	+	The Hsp90 inhibitor radicicol interacts with the ATP-binding pocket of bacterial sensor kinase PhoQ.,Guarnieri MT, Zhang L, Shen J, Zhao R J Mol Biol. 2008 May 23;379(1):82-93. Epub 2008 Mar 26. PMID:18440021<ref>PMID:18440021</ref>

-	~~===Crystal Structure~~ of ~~Salmonella Sensor Kinase PhoQ catalytic domain in complex with radicicol===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18440021}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3cgy]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Salmonella_enterica_subsp~~.~~_enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA].~~	+

	==See Also==		==See Also==
	*[[PhoP-PhoQ\|PhoP-PhoQ]]		*[[PhoP-PhoQ\|PhoP-PhoQ]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018440021<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]

OCA at 21:57, 20 October 2012

OCA — Sat, 20 Oct 2012 21:57:38 GMT

←Older revision		Revision as of 21:57, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3cgy]] is a 3 chain structure ~~of [[PhoP-PhoQ]]~~ with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA].	+	[[3cgy]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA].

	==See Also==		==See Also==

OCA: Protected "3cgy" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 14:46:22 GMT

Protected "3cgy" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:46, 27 July 2012

OCA at 14:46, 27 July 2012

OCA — Fri, 27 Jul 2012 14:46:20 GMT

←Older revision		Revision as of 14:46, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3cgy.png\|left\|200px]]		[[Image:3cgy.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3cgy", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3cgy\| PDB=3cgy \| SCENE= }}		{{STRUCTURE_3cgy\| PDB=3cgy \| SCENE= }}

	===Crystal Structure of Salmonella Sensor Kinase PhoQ catalytic domain in complex with radicicol===		===Crystal Structure of Salmonella Sensor Kinase PhoQ catalytic domain in complex with radicicol===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18440021}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18440021 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18440021}}		{{ABSTRACT_PUBMED_18440021}}

	==About this Structure==		==About this Structure==
-	~~3CGY~~ is a 3 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/~~Salmonella_typhimurium~~ Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA].	+	[[3cgy]] is a 3 chain structure of [[PhoP-PhoQ]] with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA].
		+
		+	==See Also==
		+	*[[PhoP-PhoQ\|PhoP-PhoQ]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18440021~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018440021</ref><references group="xtra"/>
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
-	[[Category: Salmonella typhimurium]]	+	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
	[[Category: Guarnieri, M T.]]		[[Category: Guarnieri, M T.]]
	[[Category: Shen, J.]]		[[Category: Shen, J.]]
Line 45:		Line 36:
	[[Category: Two-component regulatory system]]		[[Category: Two-component regulatory system]]
	[[Category: Virulence]]		[[Category: Virulence]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:38:00 2009''

OCA at 06:38, 18 February 2009

OCA — Wed, 18 Feb 2009 06:38:02 GMT

←Older revision		Revision as of 06:38, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3CGY is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA].	+	3CGY is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CGY OCA].

	==Reference==		==Reference==
-	~~The Hsp90 inhibitor radicicol interacts with the ATP-binding pocket of bacterial sensor kinase PhoQ., Guarnieri MT, Zhang L, Shen J, Zhao R, J Mol Biol. 2008 May 23;379(1):82-93. Epub 2008 Mar 26.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18440021 18440021]~~	+	<ref group="xtra">PMID:18440021</ref><references group="xtra"/>
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
	[[Category: Salmonella typhimurium]]		[[Category: Salmonella typhimurium]]
-	[[Category: Single protein]]
	[[Category: Guarnieri, M T.]]		[[Category: Guarnieri, M T.]]
	[[Category: Shen, J.]]		[[Category: Shen, J.]]
Line 47:		Line 46:
	[[Category: Virulence]]		[[Category: Virulence]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 14~~:50:~~43 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:38:00 2009''