3chu - Revision history

OCA at 09:35, 21 February 2024

OCA — Wed, 21 Feb 2024 09:35:46 GMT

←Older revision		Revision as of 09:35, 21 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3chu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CHU FirstGlance]. <br>		<table><tr><td colspan='2'>[[3chu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CHU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FEO:MU~~-~~OXO-DIIRON'>FEO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3chi\|3chi]], [[3cht\|3cht]], [[3chh\|3chh]]~~</~~div~~>~~</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aurF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66431 Streptomyces thioluteus])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3chu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chu OCA], [https://pdbe.org/3chu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3chu RCSB], [https://www.ebi.ac.uk/pdbsum/3chu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3chu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3chu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chu OCA], [https://pdbe.org/3chu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3chu RCSB], [https://www.ebi.ac.uk/pdbsum/3chu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3chu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AURF_STRTU AURF_STRTU] Involved in the biosynthesis of the polyketide antibiotic aureothin (PubMed:14700630, PubMed:15038705). Catalyzes the oxidation of p-aminobenzoate (pABA) to p-nitrobenzoate (pNBA), an unusual polyketide synthase starter unit (PubMed:15038705, PubMed:16927313, PubMed:20798054, PubMed:18458342). Reaction mechanism involves the generation of a peroxodiiron(III/III) intermediate, which effects the initial oxidation of p-aminobenzoate to p-hydroxylaminobenzoate (Ar-NHOH) (PubMed:19731912, PubMed:20798054). Ar-NHOH is then probably directly converted to the fully oxidized p-nitrobenzoate via a four-electron N-oxidation, bypassing the formation of a nitroso compound (PubMed:20798054).<ref>PMID:14700630</ref> <ref>PMID:15038705</ref> <ref>PMID:16927313</ref> <ref>PMID:18458342</ref> <ref>PMID:19731912</ref> <ref>PMID:20798054</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3chu ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3chu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	p-Aminobenzoate N-oxygenase (AurF) from Streptomyces thioluteus catalyzes the formation of unusual polyketide synthase starter unit p-nitrobenzoic acid (pNBA) from p-aminobenzoic acid (pABA) in the biosynthesis of antibiotic aureothin. AurF is a metalloenzyme, but its native enzymatic activity has not been demonstrated in vitro, and its catalytic mechanism is unclear. In addition, the nature of the cofactor remains a controversy. Here, we report the in vitro reconstitution of the AurF enzyme activity, the crystal structure of AurF in the oxidized state, and the cocrystal structure of AurF with its product pNBA. Our combined biochemical and structural analysis unequivocally indicates that AurF is a non-heme di-iron monooxygenase that catalyzes sequential oxidation of aminoarenes to nitroarenes via hydroxylamine and nitroso intermediates.
-
-	In vitro reconstitution and crystal structure of p-aminobenzoate N-oxygenase (AurF) involved in aureothin biosynthesis.,Choi YS, Zhang H, Brunzelle JS, Nair SK, Zhao H Proc Natl Acad Sci U S A. 2008 May 13;105(19):6858-63. Epub 2008 May 5. PMID:18458342<ref>PMID:18458342</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3chu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 26:
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Streptomyces thioluteus]]		[[Category: Streptomyces thioluteus]]
-	[[Category: Brunzelle~~, J S~~]]	+	[[Category: Brunzelle JS]]
-	[[Category: Nair~~, S K~~]]	+	[[Category: Nair SK]]
-	[[Category: Zhang, H]]	+	[[Category: Zhang H]]
-	~~[[Category: Di-iron oxygenase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:56, 27 January 2022

OCA — Thu, 27 Jan 2022 07:56:17 GMT

←Older revision		Revision as of 07:56, 27 January 2022
Line 1:		Line 1:

	==Crystal Structure of Di-iron Aurf==		==Crystal Structure of Di-iron Aurf==
-	<StructureSection load='3chu' size='340' side='right' caption='[[3chu]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='3chu' size='340' side='right'caption='[[3chu]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3chu]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CHU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3chu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CHU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3chi\|3chi]], [[3cht\|3cht]], [[3chh\|3chh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3chi\|3chi]], [[3cht\|3cht]], [[3chh\|3chh]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aurF ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66431 Streptomyces thioluteus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aurF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66431 Streptomyces thioluteus])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3chu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chu OCA], [~~http~~://pdbe.org/3chu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3chu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3chu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3chu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3chu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chu OCA], [https://pdbe.org/3chu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3chu RCSB], [https://www.ebi.ac.uk/pdbsum/3chu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3chu ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Streptomyces thioluteus]]		[[Category: Streptomyces thioluteus]]
	[[Category: Brunzelle, J S]]		[[Category: Brunzelle, J S]]

OCA at 14:04, 7 November 2018

OCA — Wed, 07 Nov 2018 14:04:27 GMT

←Older revision		Revision as of 14:04, 7 November 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/3chu_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/3chu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 19:28, 11 August 2016

OCA — Thu, 11 Aug 2016 19:28:05 GMT

←Older revision		Revision as of 19:28, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Di-iron Aurf==		==Crystal Structure of Di-iron Aurf==
	<StructureSection load='3chu' size='340' side='right' caption='[[3chu]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3chu' size='340' side='right' caption='[[3chu]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3chi\|3chi]], [[3cht\|3cht]], [[3chh\|3chh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3chi\|3chi]], [[3cht\|3cht]], [[3chh\|3chh]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aurF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66431 Streptomyces thioluteus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aurF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66431 Streptomyces thioluteus])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3chu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3chu RCSB], [http://www.ebi.ac.uk/pdbsum/3chu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3chu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chu OCA], [http://pdbe.org/3chu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3chu RCSB], [http://www.ebi.ac.uk/pdbsum/3chu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3chu ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3chu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3chu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:51, 20 January 2015

OCA — Tue, 20 Jan 2015 09:51:09 GMT

←Older revision		Revision as of 09:51, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3chu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CHU FirstGlance]. <br>		<table><tr><td colspan='2'>[[3chu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CHU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3chi\|3chi]], [[3cht\|3cht]], [[3chh\|3chh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3chi\|3chi]], [[3cht\|3cht]], [[3chh\|3chh]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aurF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66431 Streptomyces thioluteus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aurF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66431 Streptomyces thioluteus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3chu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3chu RCSB], [http://www.ebi.ac.uk/pdbsum/3chu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3chu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3chu RCSB], [http://www.ebi.ac.uk/pdbsum/3chu PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces thioluteus]]		[[Category: Streptomyces thioluteus]]
-	[[Category: Brunzelle, J S.]]	+	[[Category: Brunzelle, J S]]
-	[[Category: Nair, S K.]]	+	[[Category: Nair, S K]]
-	[[Category: Zhang, H.]]	+	[[Category: Zhang, H]]
	[[Category: Di-iron oxygenase]]		[[Category: Di-iron oxygenase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 21:37, 2 October 2014

OCA — Thu, 02 Oct 2014 21:37:25 GMT

←Older revision		Revision as of 21:37, 2 October 2014
Line 1:		Line 1:
-	[[~~Image:~~3chu.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Di-iron Aurf==
		+	<StructureSection load='3chu' size='340' side='right' caption='[[3chu]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3chu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CHU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3chi\|3chi]], [[3cht\|3cht]], [[3chh\|3chh]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aurF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66431 Streptomyces thioluteus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3chu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3chu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3chu RCSB], [http://www.ebi.ac.uk/pdbsum/3chu PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/3chu_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	p-Aminobenzoate N-oxygenase (AurF) from Streptomyces thioluteus catalyzes the formation of unusual polyketide synthase starter unit p-nitrobenzoic acid (pNBA) from p-aminobenzoic acid (pABA) in the biosynthesis of antibiotic aureothin. AurF is a metalloenzyme, but its native enzymatic activity has not been demonstrated in vitro, and its catalytic mechanism is unclear. In addition, the nature of the cofactor remains a controversy. Here, we report the in vitro reconstitution of the AurF enzyme activity, the crystal structure of AurF in the oxidized state, and the cocrystal structure of AurF with its product pNBA. Our combined biochemical and structural analysis unequivocally indicates that AurF is a non-heme di-iron monooxygenase that catalyzes sequential oxidation of aminoarenes to nitroarenes via hydroxylamine and nitroso intermediates.

-	~~{{STRUCTURE_3chu\| PDB=3chu \| SCENE= }}~~	+	In vitro reconstitution and crystal structure of p-aminobenzoate N-oxygenase (AurF) involved in aureothin biosynthesis.,Choi YS, Zhang H, Brunzelle JS, Nair SK, Zhao H Proc Natl Acad Sci U S A. 2008 May 13;105(19):6858-63. Epub 2008 May 5. PMID:18458342<ref>PMID:18458342</ref>

-	~~===Crystal Structure~~ of ~~Di-iron Aurf===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18458342}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3chu]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3CHU OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018458342<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptomyces thioluteus]]		[[Category: Streptomyces thioluteus]]
	[[Category: Brunzelle, J S.]]		[[Category: Brunzelle, J S.]]

OCA: Protected "3chu" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 21:48:15 GMT

Protected "3chu" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:48, 7 January 2013

OCA at 21:48, 7 January 2013

OCA — Mon, 07 Jan 2013 21:48:11 GMT

←Older revision		Revision as of 21:48, 7 January 2013
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-	{{Seed}}
	[[Image:3chu.png\|left\|200px]]		[[Image:3chu.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3chu", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3chu\| PDB=3chu \| SCENE= }}		{{STRUCTURE_3chu\| PDB=3chu \| SCENE= }}

	===Crystal Structure of Di-iron Aurf===		===Crystal Structure of Di-iron Aurf===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18458342}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18458342 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_18458342}}		{{ABSTRACT_PUBMED_18458342}}

	==About this Structure==		==About this Structure==
-	~~3CHU~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHU OCA].	+	[[3chu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHU OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18458342~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018458342</ref><references group="xtra"/>
	[[Category: Streptomyces thioluteus]]		[[Category: Streptomyces thioluteus]]
	[[Category: Brunzelle, J S.]]		[[Category: Brunzelle, J S.]]
Line 30:		Line 18:
	[[Category: Di-iron oxygenase]]		[[Category: Di-iron oxygenase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:15:45 2009''

OCA at 16:15, 17 February 2009

OCA — Tue, 17 Feb 2009 16:15:47 GMT

←Older revision		Revision as of 16:15, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3CHU is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHU OCA].	+	3CHU is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CHU OCA].

	==Reference==		==Reference==
-	In vitro reconstitution and crystal structure of p-aminobenzoate N-oxygenase (AurF) involved in aureothin biosynthesis., Choi YS, Zhang H, Brunzelle JS, Nair SK, Zhao H, Proc Natl Acad Sci U S A. 2008 May 13;105(19):6858-63. Epub 2008 May 5. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18458342 18458342]~~	+	<ref group="xtra">PMID:18458342</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Streptomyces thioluteus]]		[[Category: Streptomyces thioluteus]]
	[[Category: Brunzelle, J S.]]		[[Category: Brunzelle, J S.]]
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	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 16 08~~:56:~~38 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:15:45 2009''

OCA at 05:56, 16 July 2008

OCA — Wed, 16 Jul 2008 05:56:39 GMT

←Older revision		Revision as of 05:56, 16 July 2008
Line 1:		Line 1:
-	[[Image:3chu.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3chu.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_3chu\| PDB=3chu \| SCENE= }}		{{STRUCTURE_3chu\| PDB=3chu \| SCENE= }}

-	~~'''~~Crystal Structure of Di-iron Aurf~~'''~~	+	===Crystal Structure of Di-iron Aurf===


-	~~==Overview==~~	+	<!--
-	p-~~Aminobenzoate N~~-oxygenase (AurF) from Streptomyces thioluteus catalyzes the formation of unusual polyketide synthase starter unit p-nitrobenzoic acid (pNBA) from p-aminobenzoic acid (pABA) in the biosynthesis of antibiotic aureothin. AurF is a metalloenzyme, ~~but its native enzymatic activity has not been demonstrated in vitro, and its catalytic mechanism is unclear. In addition~~, the ~~nature of~~ the ~~cofactor remains a controversy~~. ~~Here~~, ~~we report~~ the ~~in vitro reconstitution of the AurF enzyme activity, the crystal structure of AurF in the oxidized state, and the cocrystal structure of AurF with its product pNBA~~. ~~Our combined biochemical and structural analysis unequivocally indicates that AurF is a non~~-~~heme di~~-~~iron monooxygenase that catalyzes sequential oxidation of aminoarenes to nitroarenes via hydroxylamine and nitroso intermediates.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_18458342}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18458342 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18458342}}

	==About this Structure==		==About this Structure==
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	[[Category: Di-iron oxygenase]]		[[Category: Di-iron oxygenase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~May 28 09~~:22:13 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 16 08:56:38 2008''