3clp - Revision history

OCA at 09:36, 21 February 2024

OCA — Wed, 21 Feb 2024 09:36:32 GMT

←Older revision		Revision as of 09:36, 21 February 2024
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	<StructureSection load='3clp' size='340' side='right'caption='[[3clp]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3clp' size='340' side='right'caption='[[3clp]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3clp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Rhizobium_loti Rhizobium~~ loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CLP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3clp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CLP FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CMP:ADENOSINE~~-3,~~5-CYCLIC-MONOPHOSPHATE'>CMP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1vp6\|1vp6]], [[1u12\|1u12]], [[3beh\|3beh]], [[3cl1\|3cl1]], [[3co2\|3co2]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3clp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clp OCA], [https://pdbe.org/3clp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3clp RCSB], [https://www.ebi.ac.uk/pdbsum/3clp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3clp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3clp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clp OCA], [https://pdbe.org/3clp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3clp RCSB], [https://www.ebi.ac.uk/pdbsum/3clp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3clp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CNGK1_RHILO CNGK1_RHILO]] Cyclic nucleotide-regulated potassium channel activated by cAMP.<ref>PMID:15550244</ref>	+	[https://www.uniprot.org/uniprot/CNGK1_RHILO CNGK1_RHILO] Cyclic nucleotide-regulated potassium channel activated by cAMP.<ref>PMID:15550244</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3clp ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3clp ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	MlotiK1 is a prokaryotic homolog of cyclic-nucleotide-dependent ion channels that contains an intracellular C-terminal cyclic nucleotide binding (CNB) domain. X-ray structures of the CNB domain have been solved in the absence of ligand and bound to cAMP. Both the full-length channel and CNB domain fragment are easily expressed and purified, making MlotiK1 a useful model system for dissecting activation by ligand binding. We have used X-ray crystallography to determine three new MlotiK1 CNB domain structures: a second apo configuration, a cGMP-bound structure, and a second cAMP-bound structure. In combination, the five MlotiK1 CNB domain structures provide a unique opportunity for analyzing, within a single protein, the structural differences between the apo state and the bound state, and the structural variability within each state. With this analysis as a guide, we have probed the nucleotide selectivity and importance of specific residue side chains in ligand binding and channel activation. These data help to identify ligand-protein interactions that are important for ligand dependence in MlotiK1 and, more globally, in the class of nucleotide-dependent proteins.
-
-	Structural and energetic analysis of activation by a cyclic nucleotide binding domain.,Altieri SL, Clayton GM, Silverman WR, Olivares AO, De la Cruz EM, Thomas LR, Morais-Cabral JH J Mol Biol. 2008 Sep 5;381(3):655-69. Epub 2008 Jun 10. PMID:18619611<ref>PMID:18619611</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3clp" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Rhizobium~~ loti]]	+	[[Category: Mesorhizobium loti]]
-	[[Category: Altieri~~, S L~~]]	+	[[Category: Altieri SL]]
-	[[Category: Clayton~~, G M~~]]	+	[[Category: Clayton GM]]
-	[[Category: Morais-Cabral~~, J H~~]]	+	[[Category: Morais-Cabral JH]]
-	[[Category: Thomas~~, L R]]~~	+	[[Category: Thomas LR]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Mlotik1 cyclic-nucleotide binding domain mutant 2. cyclic-amp bound~~]]	+

OCA at 19:11, 20 October 2021

OCA — Wed, 20 Oct 2021 19:11:02 GMT

←Older revision		Revision as of 19:11, 20 October 2021
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	<StructureSection load='3clp' size='340' side='right'caption='[[3clp]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3clp' size='340' side='right'caption='[[3clp]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3clp]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Rhizobium_loti Rhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CLP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3clp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_loti Rhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CLP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vp6\|1vp6]], [[1u12\|1u12]], [[3beh\|3beh]], [[3cl1\|3cl1]], [[3co2\|3co2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1vp6\|1vp6]], [[1u12\|1u12]], [[3beh\|3beh]], [[3cl1\|3cl1]], [[3co2\|3co2]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3clp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clp OCA], [~~http~~://pdbe.org/3clp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3clp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3clp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3clp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3clp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clp OCA], [https://pdbe.org/3clp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3clp RCSB], [https://www.ebi.ac.uk/pdbsum/3clp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3clp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CNGK1_RHILO CNGK1_RHILO]] Cyclic nucleotide-regulated potassium channel activated by cAMP.<ref>PMID:15550244</ref>	+	[[https://www.uniprot.org/uniprot/CNGK1_RHILO CNGK1_RHILO]] Cyclic nucleotide-regulated potassium channel activated by cAMP.<ref>PMID:15550244</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
-	*[[Ion channels\|Ion channels]]	+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:53, 24 July 2019

OCA — Wed, 24 Jul 2019 07:53:16 GMT

←Older revision		Revision as of 07:53, 24 July 2019
Line 1:		Line 1:

	==M. loti cyclic-nucleotide binding domain mutant 2==		==M. loti cyclic-nucleotide binding domain mutant 2==
-	<StructureSection load='3clp' size='340' side='right' caption='[[3clp]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3clp' size='340' side='right'caption='[[3clp]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3clp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizobium_loti Rhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLP FirstGlance]. <br>		<table><tr><td colspan='2'>[[3clp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizobium_loti Rhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLP FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/3clp_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/3clp_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 3clp" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3clp" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ion channels\|Ion channels]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Rhizobium loti]]		[[Category: Rhizobium loti]]
	[[Category: Altieri, S L]]		[[Category: Altieri, S L]]

OCA at 08:29, 25 October 2017

OCA — Wed, 25 Oct 2017 08:29:49 GMT

←Older revision		Revision as of 08:29, 25 October 2017
Line 3:		Line 3:
	<StructureSection load='3clp' size='340' side='right' caption='[[3clp]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3clp' size='340' side='right' caption='[[3clp]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3clp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mesorhizobium_loti Mesorhizobium~~ loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3clp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizobium_loti Rhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vp6\|1vp6]], [[1u12\|1u12]], [[3beh\|3beh]], [[3cl1\|3cl1]], [[3co2\|3co2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vp6\|1vp6]], [[1u12\|1u12]], [[3beh\|3beh]], [[3cl1\|3cl1]], [[3co2\|3co2]]</td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mesorhizobium~~ loti]]	+	[[Category: Rhizobium loti]]
	[[Category: Altieri, S L]]		[[Category: Altieri, S L]]
	[[Category: Clayton, G M]]		[[Category: Clayton, G M]]

OCA at 15:40, 11 August 2016

OCA — Thu, 11 Aug 2016 15:40:35 GMT

←Older revision		Revision as of 15:40, 11 August 2016
Line 1:		Line 1:
		+
	==M. loti cyclic-nucleotide binding domain mutant 2==		==M. loti cyclic-nucleotide binding domain mutant 2==
	<StructureSection load='3clp' size='340' side='right' caption='[[3clp]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3clp' size='340' side='right' caption='[[3clp]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vp6\|1vp6]], [[1u12\|1u12]], [[3beh\|3beh]], [[3cl1\|3cl1]], [[3co2\|3co2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vp6\|1vp6]], [[1u12\|1u12]], [[3beh\|3beh]], [[3cl1\|3cl1]], [[3co2\|3co2]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3clp RCSB], [http://www.ebi.ac.uk/pdbsum/3clp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clp OCA], [http://pdbe.org/3clp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3clp RCSB], [http://www.ebi.ac.uk/pdbsum/3clp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3clp ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CNGK1_RHILO CNGK1_RHILO]] Cyclic nucleotide-regulated potassium channel activated by cAMP.<ref>PMID:15550244</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 15:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3clp ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3clp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:40, 20 January 2015

OCA — Tue, 20 Jan 2015 09:40:36 GMT

←Older revision		Revision as of 09:40, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3clp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLP FirstGlance]. <br>		<table><tr><td colspan='2'>[[3clp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLP FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vp6\|1vp6]], [[1u12\|1u12]], [[3beh\|3beh]], [[3cl1\|3cl1]], [[3co2\|3co2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vp6\|1vp6]], [[1u12\|1u12]], [[3beh\|3beh]], [[3cl1\|3cl1]], [[3co2\|3co2]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3clp RCSB], [http://www.ebi.ac.uk/pdbsum/3clp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3clp RCSB], [http://www.ebi.ac.uk/pdbsum/3clp PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Mesorhizobium loti]]		[[Category: Mesorhizobium loti]]
-	[[Category: Altieri, S L.]]	+	[[Category: Altieri, S L]]
-	[[Category: Clayton, G M.]]	+	[[Category: Clayton, G M]]
-	[[Category: Morais-Cabral, J H.]]	+	[[Category: Morais-Cabral, J H]]
-	[[Category: Thomas, L R.]]	+	[[Category: Thomas, L R]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Mlotik1 cyclic-nucleotide binding domain mutant 2. cyclic-amp bound]]		[[Category: Mlotik1 cyclic-nucleotide binding domain mutant 2. cyclic-amp bound]]

OCA at 21:27, 2 October 2014

OCA — Thu, 02 Oct 2014 21:27:36 GMT

←Older revision		Revision as of 21:27, 2 October 2014
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-	[[~~Image:~~3clp.~~png~~\|~~left~~\|200px]]	+	==M. loti cyclic-nucleotide binding domain mutant 2==
		+	<StructureSection load='3clp' size='340' side='right' caption='[[3clp]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3clp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLP FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vp6\|1vp6]], [[1u12\|1u12]], [[3beh\|3beh]], [[3cl1\|3cl1]], [[3co2\|3co2]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3clp RCSB], [http://www.ebi.ac.uk/pdbsum/3clp PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/3clp_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MlotiK1 is a prokaryotic homolog of cyclic-nucleotide-dependent ion channels that contains an intracellular C-terminal cyclic nucleotide binding (CNB) domain. X-ray structures of the CNB domain have been solved in the absence of ligand and bound to cAMP. Both the full-length channel and CNB domain fragment are easily expressed and purified, making MlotiK1 a useful model system for dissecting activation by ligand binding. We have used X-ray crystallography to determine three new MlotiK1 CNB domain structures: a second apo configuration, a cGMP-bound structure, and a second cAMP-bound structure. In combination, the five MlotiK1 CNB domain structures provide a unique opportunity for analyzing, within a single protein, the structural differences between the apo state and the bound state, and the structural variability within each state. With this analysis as a guide, we have probed the nucleotide selectivity and importance of specific residue side chains in ligand binding and channel activation. These data help to identify ligand-protein interactions that are important for ligand dependence in MlotiK1 and, more globally, in the class of nucleotide-dependent proteins.

-	~~{{STRUCTURE_3clp\| PDB=3clp \| SCENE= }}~~	+	Structural and energetic analysis of activation by a cyclic nucleotide binding domain.,Altieri SL, Clayton GM, Silverman WR, Olivares AO, De la Cruz EM, Thomas LR, Morais-Cabral JH J Mol Biol. 2008 Sep 5;381(3):655-69. Epub 2008 Jun 10. PMID:18619611<ref>PMID:18619611</ref>

-	~~===M. loti cyclic-nucleotide binding domain mutant 2===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18619611}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3clp]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mesorhizobium_loti Mesorhizobium loti]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018619611<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mesorhizobium loti]]		[[Category: Mesorhizobium loti]]
	[[Category: Altieri, S L.]]		[[Category: Altieri, S L.]]

OCA: Protected "3clp" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 18:58:34 GMT

Protected "3clp" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:58, 7 January 2013

OCA at 18:58, 7 January 2013

OCA — Mon, 07 Jan 2013 18:58:32 GMT

←Older revision		Revision as of 18:58, 7 January 2013
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-	{{Seed}}
	[[Image:3clp.png\|left\|200px]]		[[Image:3clp.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3clp", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3clp\| PDB=3clp \| SCENE= }}		{{STRUCTURE_3clp\| PDB=3clp \| SCENE= }}

	===M. loti cyclic-nucleotide binding domain mutant 2===		===M. loti cyclic-nucleotide binding domain mutant 2===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18619611}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18619611 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_18619611}}		{{ABSTRACT_PUBMED_18619611}}

	==About this Structure==		==About this Structure==
-	~~3CLP~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA].	+	[[3clp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18619611~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018619611</ref><references group="xtra"/>
	[[Category: Mesorhizobium loti]]		[[Category: Mesorhizobium loti]]
	[[Category: Altieri, S L.]]		[[Category: Altieri, S L.]]
Line 31:		Line 19:
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Mlotik1 cyclic-nucleotide binding domain mutant 2. cyclic-amp bound]]		[[Category: Mlotik1 cyclic-nucleotide binding domain mutant 2. cyclic-amp bound]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:15:11 2009''

OCA at 03:15, 18 February 2009

OCA — Wed, 18 Feb 2009 03:15:12 GMT

←Older revision		Revision as of 03:15, 18 February 2009
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	{{Seed}}		{{Seed}}
-	[[Image:3clp.~~jpg~~\|left\|200px]]	+	[[Image:3clp.png\|left\|200px]]

	<!--		<!--
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	==About this Structure==		==About this Structure==
-	3CLP is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA].	+	3CLP is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLP OCA].

	==Reference==		==Reference==
-	Structural and energetic analysis of activation by a cyclic nucleotide binding domain., Altieri SL, Clayton GM, Silverman WR, Olivares AO, De la Cruz EM, Thomas LR, Morais-Cabral JH, J Mol Biol. 2008 Sep 5;381(3):655-69. Epub 2008 Jun 10. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18619611 18619611]~~	+	<ref group="xtra">PMID:18619611</ref><references group="xtra"/>
	[[Category: Mesorhizobium loti]]		[[Category: Mesorhizobium loti]]
-	[[Category: Single protein]]
	[[Category: Altieri, S L.]]		[[Category: Altieri, S L.]]
	[[Category: Clayton, G M.]]		[[Category: Clayton, G M.]]
Line 33:		Line 32:
	[[Category: Mlotik1 cyclic-nucleotide binding domain mutant 2. cyclic-amp bound]]		[[Category: Mlotik1 cyclic-nucleotide binding domain mutant 2. cyclic-amp bound]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Aug 6 12~~:58:~~57 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:15:11 2009''