3clu - Revision history

OCA at 12:30, 30 August 2023

OCA — Wed, 30 Aug 2023 12:30:22 GMT

←Older revision		Revision as of 12:30, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='3clu' size='340' side='right'caption='[[3clu]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3clu' size='340' side='right'caption='[[3clu]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3clu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_53528 Atcc 53528~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CLU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3clu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CLU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>~~, ~~<scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1o97\|1o97]], [[3clr\|3clr]], [[3cls\|3cls]], [[3clt\|3clt]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td><td class="sblockDat">etfB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ATCC 53528]), etfA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ATCC 53528])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [https://pdbe.org/3clu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [https://www.ebi.ac.uk/pdbsum/3clu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3clu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [https://pdbe.org/3clu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [https://www.ebi.ac.uk/pdbsum/3clu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3clu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ETFB_METME ETFB_METME]] The electron transfer flavoprotein of this bacterium serves as an electron acceptor specifically for trimethylamine dehydrogenase. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). [[https://www.uniprot.org/uniprot/ETFA_METME ETFA_METME]] The electron transfer flavoprotein of this bacterium serves as an electron acceptor specifically for trimethylamine dehydrogenase. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).	+	[https://www.uniprot.org/uniprot/ETFA_METME ETFA_METME] The electron transfer flavoprotein of this bacterium serves as an electron acceptor specifically for trimethylamine dehydrogenase. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 53528]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Katona, G~~]]	+	[[Category: Methylophilus methylotrophus]]
-	[[Category: ~~Leys, D~~]]	+	[[Category: Katona G]]
-	[[Category: ~~Dynamic interface]]~~	+	[[Category: Leys D]]
-	~~[[Category: Electron transfer]]~~	+
-	~~[[Category: Electron transport]]~~	+
-	~~[[Category: Etf]]~~	+
-	~~[[Category: Fad]]~~	+
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Tmadh]]~~	+
-	~~[[Category: Transport~~]]	+

OCA at 19:11, 20 October 2021

OCA — Wed, 20 Oct 2021 19:11:15 GMT

←Older revision		Revision as of 19:11, 20 October 2021
Line 1:		Line 1:

	==Crystal structure of the R236K mutant from Methylophilus methylotrophus ETF==		==Crystal structure of the R236K mutant from Methylophilus methylotrophus ETF==
-	<StructureSection load='3clu' size='340' side='right' caption='[[3clu]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='3clu' size='340' side='right'caption='[[3clu]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3clu]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_53528 Atcc 53528]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CLU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3clu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_53528 Atcc 53528]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CLU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o97\|1o97]], [[3clr\|3clr]], [[3cls\|3cls]], [[3clt\|3clt]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1o97\|1o97]], [[3clr\|3clr]], [[3cls\|3cls]], [[3clt\|3clt]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">etfB ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ATCC 53528]), etfA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ATCC 53528])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">etfB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ATCC 53528]), etfA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ATCC 53528])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [~~http~~://pdbe.org/3clu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3clu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3clu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [https://pdbe.org/3clu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [https://www.ebi.ac.uk/pdbsum/3clu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3clu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ETFB_METME ETFB_METME]] The electron transfer flavoprotein of this bacterium serves as an electron acceptor specifically for trimethylamine dehydrogenase. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). [[~~http~~://www.uniprot.org/uniprot/ETFA_METME ETFA_METME]] The electron transfer flavoprotein of this bacterium serves as an electron acceptor specifically for trimethylamine dehydrogenase. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).	+	[[https://www.uniprot.org/uniprot/ETFB_METME ETFB_METME]] The electron transfer flavoprotein of this bacterium serves as an electron acceptor specifically for trimethylamine dehydrogenase. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). [[https://www.uniprot.org/uniprot/ETFA_METME ETFA_METME]] The electron transfer flavoprotein of this bacterium serves as an electron acceptor specifically for trimethylamine dehydrogenase. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/3clu_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/3clu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 53528]]		[[Category: Atcc 53528]]
		+	[[Category: Large Structures]]
	[[Category: Katona, G]]		[[Category: Katona, G]]
	[[Category: Leys, D]]		[[Category: Leys, D]]

OCA at 08:30, 25 October 2017

OCA — Wed, 25 Oct 2017 08:30:05 GMT

←Older revision		Revision as of 08:30, 25 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of the R236K mutant from Methylophilus methylotrophus ETF==		==Crystal structure of the R236K mutant from Methylophilus methylotrophus ETF==
	<StructureSection load='3clu' size='340' side='right' caption='[[3clu]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3clu' size='340' side='right' caption='[[3clu]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o97\|1o97]], [[3clr\|3clr]], [[3cls\|3cls]], [[3clt\|3clt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o97\|1o97]], [[3clr\|3clr]], [[3cls\|3cls]], [[3clt\|3clt]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">etfB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ATCC 53528]), etfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ATCC 53528])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">etfB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ATCC 53528]), etfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ATCC 53528])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [http://pdbe.org/3clu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [http://www.ebi.ac.uk/pdbsum/3clu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [http://pdbe.org/3clu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [http://www.ebi.ac.uk/pdbsum/3clu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3clu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 13:17, 7 February 2016

OCA — Sun, 07 Feb 2016 13:17:20 GMT

←Older revision		Revision as of 13:17, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='3clu' size='340' side='right' caption='[[3clu]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3clu' size='340' side='right' caption='[[3clu]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3clu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Methylophilus_methylotrophus Methylophilus methylotrophus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3clu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_53528 Atcc 53528]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o97\|1o97]], [[3clr\|3clr]], [[3cls\|3cls]], [[3clt\|3clt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o97\|1o97]], [[3clr\|3clr]], [[3cls\|3cls]], [[3clt\|3clt]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">etfB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ~~Methylophilus methylotrophus~~]), etfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ~~Methylophilus methylotrophus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">etfB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ATCC 53528]), etfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 ATCC 53528])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [http://www.ebi.ac.uk/pdbsum/3clu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [http://pdbe.org/3clu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [http://www.ebi.ac.uk/pdbsum/3clu PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3clu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3clu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Methylophilus methylotrophus~~]]	+	[[Category: Atcc 53528]]
	[[Category: Katona, G]]		[[Category: Katona, G]]
	[[Category: Leys, D]]		[[Category: Leys, D]]

OCA at 15:21, 24 December 2014

OCA — Wed, 24 Dec 2014 15:21:26 GMT

←Older revision		Revision as of 15:21, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [http://www.ebi.ac.uk/pdbsum/3clu PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [http://www.ebi.ac.uk/pdbsum/3clu PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ETFB_METME ETFB_METME]] The electron transfer flavoprotein of this bacterium serves as an electron acceptor specifically for trimethylamine dehydrogenase. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). [[http://www.uniprot.org/uniprot/ETFA_METME ETFA_METME]] The electron transfer flavoprotein of this bacterium serves as an electron acceptor specifically for trimethylamine dehydrogenase. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Methylophilus methylotrophus]]		[[Category: Methylophilus methylotrophus]]
-	[[Category: Katona, G.]]	+	[[Category: Katona, G]]
-	[[Category: Leys, D.]]	+	[[Category: Leys, D]]
	[[Category: Dynamic interface]]		[[Category: Dynamic interface]]
	[[Category: Electron transfer]]		[[Category: Electron transfer]]

OCA at 06:39, 10 October 2014

OCA — Fri, 10 Oct 2014 06:39:07 GMT

←Older revision		Revision as of 06:39, 10 October 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3clu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLU FirstGlance]. <br>		<table><tr><td colspan='2'>[[3clu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o97\|1o97]], [[3clr\|3clr]], [[3cls\|3cls]], [[3clt\|3clt]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o97\|1o97]], [[3clr\|3clr]], [[3cls\|3cls]], [[3clt\|3clt]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">etfB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 Methylophilus methylotrophus]), etfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 Methylophilus methylotrophus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">etfB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 Methylophilus methylotrophus]), etfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 Methylophilus methylotrophus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [http://www.ebi.ac.uk/pdbsum/3clu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [http://www.ebi.ac.uk/pdbsum/3clu PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 24:
	Probing the dynamic interface between trimethylamine dehydrogenase (TMADH) and electron transferring flavoprotein (ETF) in the TMADH-2ETF complex: role of the Arg-alpha237 (ETF) and Tyr-442 (TMADH) residue pair.,Burgess SG, Messiha HL, Katona G, Rigby SE, Leys D, Scrutton NS Biochemistry. 2008 May 6;47(18):5168-81. Epub 2008 Apr 12. PMID:18407658<ref>PMID:18407658</ref>		Probing the dynamic interface between trimethylamine dehydrogenase (TMADH) and electron transferring flavoprotein (ETF) in the TMADH-2ETF complex: role of the Arg-alpha237 (ETF) and Tyr-442 (TMADH) residue pair.,Burgess SG, Messiha HL, Katona G, Rigby SE, Leys D, Scrutton NS Biochemistry. 2008 May 6;47(18):5168-81. Epub 2008 Apr 12. PMID:18407658<ref>PMID:18407658</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 09:39, 21 May 2014

OCA — Wed, 21 May 2014 09:39:34 GMT

←Older revision		Revision as of 09:39, 21 May 2014
Line 1:		Line 1:
-	[[~~Image:~~3clu.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of the R236K mutant from Methylophilus methylotrophus ETF==
		+	<StructureSection load='3clu' size='340' side='right' caption='[[3clu]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3clu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CLU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o97\|1o97]], [[3clr\|3clr]], [[3cls\|3cls]], [[3clt\|3clt]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">etfB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 Methylophilus methylotrophus]), etfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=17 Methylophilus methylotrophus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3clu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3clu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3clu RCSB], [http://www.ebi.ac.uk/pdbsum/3clu PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/3clu_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have used multiple solution state techniques and crystallographic analysis to investigate the importance of a putative transient interaction formed between Arg-alpha237 in electron transferring flavoprotein (ETF) and Tyr-442 in trimethylamine dehydrogenase (TMADH) in complex assembly, electron transfer, and structural imprinting of ETF by TMADH. We have isolated four mutant forms of ETF altered in the identity of the residue at position 237 (alphaR237A, alphaR237K, alphaR237C, and alphaR237E) and with each form studied electron transfer from TMADH to ETF, investigated the reduction potentials of the bound ETF cofactor, and analyzed complex formation. We show that mutation of Arg-alpha237 substantially destabilizes the semiquinone couple of the bound FAD and impedes electron transfer from TMADH to ETF. Crystallographic structures of the mutant ETF proteins indicate that mutation does not perturb the overall structure of ETF, but leads to disruption of an electrostatic network at an ETF domain boundary that likely affects the dynamic properties of ETF in the crystal and in solution. We show that Arg-alpha237 is required for TMADH to structurally imprint the as-purified semiquinone form of wild-type ETF and that the ability of TMADH to facilitate this structural reorganization is lost following (i) redox cycling of ETF, or simple conversion to the oxidized form, and (ii) mutagenesis of Arg-alpha237. We discuss this result in light of recent apparent conflict in the literature relating to the structural imprinting of wild-type ETF. Our studies support a mechanism of electron transfer by conformational sampling as advanced from our previous analysis of the crystal structure of the TMADH-2ETF complex [Leys, D. , Basran, J. , Sutcliffe, M. J., and Scrutton, N. S. (2003) Nature Struct. Biol. 10, 219-225] and point to a key role for the Tyr-442 (TMADH) and Arg-alpha237 (ETF) residue pair in transiently stabilizing productive electron transfer configurations. Our work also points to the importance of Arg-alpha237 in controlling the thermodynamics of electron transfer, the dynamics of ETF, and the protection of reducing equivalents following disassembly of the TMADH-2ETF complex.

-	~~<!--~~	+	Probing the dynamic interface between trimethylamine dehydrogenase (TMADH) and electron transferring flavoprotein (ETF) in the TMADH-2ETF complex: role of the Arg-alpha237 (ETF) and Tyr-442 (TMADH) residue pair.,Burgess SG, Messiha HL, Katona G, Rigby SE, Leys D, Scrutton NS Biochemistry. 2008 May 6;47(18):5168-81. Epub 2008 Apr 12. PMID:18407658<ref>PMID:18407658</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3clu", creates~~ the ~~"Structure Box" on the page.~~	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter~~ (~~which sets~~ the ~~initial scene displayed when~~ the ~~page is loaded~~),	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3clu\| PDB=3clu \| SCENE= }}~~	+

-	~~===Crystal structure~~ of the ~~R236K mutant from Methylophilus methylotrophus ETF===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_18407658}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 18407658 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_18407658}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3clu]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018407658<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Methylophilus methylotrophus]]		[[Category: Methylophilus methylotrophus]]
	[[Category: Katona, G.]]		[[Category: Katona, G.]]

OCA: Protected "3clu" [edit=sysop:move=sysop]

OCA — Wed, 05 Oct 2011 05:38:01 GMT

Protected "3clu" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:38, 5 October 2011

OCA at 05:37, 5 October 2011

OCA — Wed, 05 Oct 2011 05:37:59 GMT

←Older revision		Revision as of 05:37, 5 October 2011
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3clu.png\|left\|200px]]		[[Image:3clu.png\|left\|200px]]

Line 12:		Line 11:
	===Crystal structure of the R236K mutant from Methylophilus methylotrophus ETF===		===Crystal structure of the R236K mutant from Methylophilus methylotrophus ETF===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18407658}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18407658 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18407658}}

	==About this Structure==		==About this Structure==
-	~~3CLU~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA].	+	[[3clu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:018407658</ref><references group="xtra"/>
	[[Category: Methylophilus methylotrophus]]		[[Category: Methylophilus methylotrophus]]
	[[Category: Katona, G.]]		[[Category: Katona, G.]]
Line 26:		Line 34:
	[[Category: Tmadh]]		[[Category: Tmadh]]
	[[Category: Transport]]		[[Category: Transport]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:03:39 2009''

OCA at 12:03, 16 February 2009

OCA — Mon, 16 Feb 2009 12:03:42 GMT

←Older revision		Revision as of 12:03, 16 February 2009
Line 1:		Line 1:
-	[[Image:3clu.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3clu.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_3clu\| PDB=3clu \| SCENE= }}		{{STRUCTURE_3clu\| PDB=3clu \| SCENE= }}

-	~~'''~~Crystal structure of the R236K mutant from Methylophilus methylotrophus ETF~~'''~~	+	===Crystal structure of the R236K mutant from Methylophilus methylotrophus ETF===


	==About this Structure==		==About this Structure==
-	3CLU is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA].	+	3CLU is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CLU OCA].
	[[Category: Methylophilus methylotrophus]]		[[Category: Methylophilus methylotrophus]]
-	[[Category: Protein complex]]
	[[Category: Katona, G.]]		[[Category: Katona, G.]]
	[[Category: Leys, D.]]		[[Category: Leys, D.]]
Line 26:		Line 26:
	[[Category: Tmadh]]		[[Category: Tmadh]]
	[[Category: Transport]]		[[Category: Transport]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:03:39 2009''