3cm8 - Revision history

OCA at 11:16, 21 February 2024

OCA — Wed, 21 Feb 2024 11:16:20 GMT

←Older revision		Revision as of 11:16, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3cm8' size='340' side='right'caption='[[3cm8]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='3cm8' size='340' side='right'caption='[[3cm8]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cm8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~9infa 9infa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CM8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cm8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus Influenza A virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CM8 FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>~~[https://en.wikipedia.org/wiki/RNA~~-~~directed_RNA_polymerase RNA-directed RNA polymerase]~~, ~~with EC number~~ [~~https://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~2.~~7.7.48 2.7.7.48] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.899Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm8 OCA], [https://pdbe.org/3cm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cm8 RCSB], [https://www.ebi.ac.uk/pdbsum/3cm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cm8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm8 OCA], [https://pdbe.org/3cm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cm8 RCSB], [https://www.ebi.ac.uk/pdbsum/3cm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cm8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~RDRP_I97A1 RDRP_I97A1~~]] RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.[UniProtKB:P03431]	+	[https://www.uniprot.org/uniprot/Q9EA60_9INFA Q9EA60_9INFA]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The recent emergence of highly pathogenic avian influenza A virus strains with subtype H5N1 pose a global threat to human health. Elucidation of the underlying mechanisms of viral replication is critical for development of anti-influenza virus drugs. The influenza RNA-dependent RNA polymerase (RdRp) heterotrimer has crucial roles in viral RNA replication and transcription. It contains three proteins: PA, PB1 and PB2. PB1 harbours polymerase and endonuclease activities and PB2 is responsible for cap binding; PA is implicated in RNA replication and proteolytic activity, although its function is less clearly defined. Here we report the 2.9 angstrom structure of avian H5N1 influenza A virus PA (PA(C), residues 257-716) in complex with the PA-binding region of PB1 (PB1(N), residues 1-25). PA(C) has a fold resembling a dragon's head with PB1(N) clamped into its open 'jaws'. PB1(N) is a known inhibitor that blocks assembly of the polymerase heterotrimer and abolishes viral replication. Our structure provides details for the binding of PB1(N) to PA(C) at the atomic level, demonstrating a potential target for novel anti-influenza therapeutics. We also discuss a potential nucleotide binding site and the roles of some known residues involved in polymerase activity. Furthermore, to explore the role of PA in viral replication and transcription, we propose a model for the influenza RdRp heterotrimer by comparing PA(C) with the lambda3 reovirus polymerase structure, and docking the PA(C) structure into an available low resolution electron microscopy map.	+
-		+
-	Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus.,He X, Zhou J, Bartlam M, Zhang R, Ma J, Lou Z, Li X, Li J, Joachimiak A, Zeng Z, Ge R, Rao Z, Liu Y Nature. 2008 Aug 28;454(7208):1123-6. Epub 2008 Jul 9. PMID:18615018<ref>PMID:18615018</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3cm8" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]		*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Influenza A virus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: RNA-directed RNA polymerase]]~~	+	[[Category: He X]]
-	[[Category: He, X]]	+	[[Category: Liu Y]]
-	[[Category: Liu, Y]]	+	[[Category: Ma J]]
-	[[Category: Ma, J]]	+	[[Category: Rao Z]]
-	[[Category: Rao, Z]]	+	[[Category: Zeng Z]]
-	[[Category: Zeng, Z]]	+	[[Category: Zhang R]]
-	[[Category: Zhang, R]]	+	[[Category: Zhou J]]
-	[[Category: Zhou, J]]	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Nucleotidyltransferase]]~~	+
-	~~[[Category: Protein-peptide complex]]~~	+
-	~~[[Category: Rna binding protein-transferase complex]]~~	+
-	~~[[Category: Rna replication]]~~	+
-	~~[[Category: Rna-directed rna polymerase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:39, 18 May 2022

OCA — Wed, 18 May 2022 10:39:48 GMT

←Older revision		Revision as of 10:39, 18 May 2022
Line 3:		Line 3:
	<StructureSection load='3cm8' size='340' side='right'caption='[[3cm8]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='3cm8' size='340' side='right'caption='[[3cm8]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cm8]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/9infa 9infa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3CM8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cm8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/9infa 9infa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CM8 FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm8 OCA], [~~http~~://pdbe.org/3cm8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3cm8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3cm8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3cm8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm8 OCA], [https://pdbe.org/3cm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cm8 RCSB], [https://www.ebi.ac.uk/pdbsum/3cm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cm8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RDRP_I97A1 RDRP_I97A1]] RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.[UniProtKB:P03431]	+	[[https://www.uniprot.org/uniprot/RDRP_I97A1 RDRP_I97A1]] RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.[UniProtKB:P03431]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 10:28, 12 August 2020

OCA — Wed, 12 Aug 2020 10:28:03 GMT

←Older revision		Revision as of 10:28, 12 August 2020
Line 1:		Line 1:

	==A RNA polymerase subunit structure from virus==		==A RNA polymerase subunit structure from virus==
-	<StructureSection load='3cm8' size='340' side='right' caption='[[3cm8]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='3cm8' size='340' side='right'caption='[[3cm8]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cm8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/9infa 9infa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CM8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cm8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/9infa 9infa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3CM8 FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm8 OCA], [http://pdbe.org/3cm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cm8 RCSB], [http://www.ebi.ac.uk/pdbsum/3cm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cm8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm8 OCA], [http://pdbe.org/3cm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cm8 RCSB], [http://www.ebi.ac.uk/pdbsum/3cm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cm8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[RNA polymerase\|RNA polymerase]]	+	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: RNA-directed RNA polymerase]]		[[Category: RNA-directed RNA polymerase]]
	[[Category: He, X]]		[[Category: He, X]]

OCA at 08:46, 5 August 2016

OCA — Fri, 05 Aug 2016 08:46:09 GMT

←Older revision		Revision as of 08:46, 5 August 2016
Line 1:		Line 1:
		+
	==A RNA polymerase subunit structure from virus==		==A RNA polymerase subunit structure from virus==
	<StructureSection load='3cm8' size='340' side='right' caption='[[3cm8]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='3cm8' size='340' side='right' caption='[[3cm8]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cm8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Influenza_a_virus Influenza a virus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CM8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cm8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/9infa 9infa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CM8 FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cm8 RCSB], [http://www.ebi.ac.uk/pdbsum/3cm8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm8 OCA], [http://pdbe.org/3cm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cm8 RCSB], [http://www.ebi.ac.uk/pdbsum/3cm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cm8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3cm8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Influenza a virus]]
	[[Category: RNA-directed RNA polymerase]]		[[Category: RNA-directed RNA polymerase]]
	[[Category: He, X]]		[[Category: He, X]]

OCA at 17:23, 25 December 2014

OCA — Thu, 25 Dec 2014 17:23:36 GMT

←Older revision		Revision as of 17:23, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cm8 RCSB], [http://www.ebi.ac.uk/pdbsum/3cm8 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cm8 RCSB], [http://www.ebi.ac.uk/pdbsum/3cm8 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RDRP_I97A1 RDRP_I97A1]] RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.[UniProtKB:P03431]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[RNA polymerase\|RNA polymerase]]
	== References ==		== References ==
	<references/>		<references/>
Line 20:		Line 25:
	[[Category: Influenza a virus]]		[[Category: Influenza a virus]]
	[[Category: RNA-directed RNA polymerase]]		[[Category: RNA-directed RNA polymerase]]
-	[[Category: He, X.]]	+	[[Category: He, X]]
-	[[Category: Liu, Y.]]	+	[[Category: Liu, Y]]
-	[[Category: Ma, J.]]	+	[[Category: Ma, J]]
-	[[Category: Rao, Z.]]	+	[[Category: Rao, Z]]
-	[[Category: Zeng, Z.]]	+	[[Category: Zeng, Z]]
-	[[Category: Zhang, R.]]	+	[[Category: Zhang, R]]
-	[[Category: Zhou, J.]]	+	[[Category: Zhou, J]]
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]
	[[Category: Nucleotidyltransferase]]		[[Category: Nucleotidyltransferase]]

OCA at 09:23, 5 November 2014

OCA — Wed, 05 Nov 2014 09:23:36 GMT

←Older revision		Revision as of 09:23, 5 November 2014
Line 1:		Line 1:
-	[[~~Image~~:3cm8.~~png\|left\|200px~~]]	+	==A RNA polymerase subunit structure from virus==
		+	<StructureSection load='3cm8' size='340' side='right' caption='[[3cm8]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3cm8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CM8 FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cm8 RCSB], [http://www.ebi.ac.uk/pdbsum/3cm8 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The recent emergence of highly pathogenic avian influenza A virus strains with subtype H5N1 pose a global threat to human health. Elucidation of the underlying mechanisms of viral replication is critical for development of anti-influenza virus drugs. The influenza RNA-dependent RNA polymerase (RdRp) heterotrimer has crucial roles in viral RNA replication and transcription. It contains three proteins: PA, PB1 and PB2. PB1 harbours polymerase and endonuclease activities and PB2 is responsible for cap binding; PA is implicated in RNA replication and proteolytic activity, although its function is less clearly defined. Here we report the 2.9 angstrom structure of avian H5N1 influenza A virus PA (PA(C), residues 257-716) in complex with the PA-binding region of PB1 (PB1(N), residues 1-25). PA(C) has a fold resembling a dragon's head with PB1(N) clamped into its open 'jaws'. PB1(N) is a known inhibitor that blocks assembly of the polymerase heterotrimer and abolishes viral replication. Our structure provides details for the binding of PB1(N) to PA(C) at the atomic level, demonstrating a potential target for novel anti-influenza therapeutics. We also discuss a potential nucleotide binding site and the roles of some known residues involved in polymerase activity. Furthermore, to explore the role of PA in viral replication and transcription, we propose a model for the influenza RdRp heterotrimer by comparing PA(C) with the lambda3 reovirus polymerase structure, and docking the PA(C) structure into an available low resolution electron microscopy map.

-	~~{{STRUCTURE_3cm8\| PDB=3cm8 \| SCENE= }}~~	+	Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus.,He X, Zhou J, Bartlam M, Zhang R, Ma J, Lou Z, Li X, Li J, Joachimiak A, Zeng Z, Ge R, Rao Z, Liu Y Nature. 2008 Aug 28;454(7208):1123-6. Epub 2008 Jul 9. PMID:18615018<ref>PMID:18615018</ref>

-	~~===A RNA polymerase subunit structure from virus===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18615018}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3cm8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki~~/~~Influenza_a_virus Influenza~~ a ~~virus]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3CM8 OCA].~~	+	</StructureSection>
-		+
-	==~~See Also==~~	+
-	*[[Polymerase acidic protein\|Polymerase acidic protein]]	+
-		+
-	~~==Reference~~==	+
-	<~~ref group="xtra">PMID:018615018<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Influenza a virus]]		[[Category: Influenza a virus]]
	[[Category: RNA-directed RNA polymerase]]		[[Category: RNA-directed RNA polymerase]]

OCA at 19:24, 20 October 2012

OCA — Sat, 20 Oct 2012 19:24:13 GMT

←Older revision		Revision as of 19:24, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3cm8]] is a 2 chain structure ~~of [[Polymerase acidic protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA].	+	[[3cm8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA].

	==See Also==		==See Also==

OCA: Protected "3cm8" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 07:14:55 GMT

Protected "3cm8" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:14, 27 July 2012

OCA at 07:14, 27 July 2012

OCA — Fri, 27 Jul 2012 07:14:53 GMT

←Older revision		Revision as of 07:14, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3cm8.png\|left\|200px]]		[[Image:3cm8.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3cm8", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3cm8\| PDB=3cm8 \| SCENE= }}		{{STRUCTURE_3cm8\| PDB=3cm8 \| SCENE= }}

	===A RNA polymerase subunit structure from virus===		===A RNA polymerase subunit structure from virus===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18615018}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18615018 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18615018}}		{{ABSTRACT_PUBMED_18615018}}

	==About this Structure==		==About this Structure==
-	~~3CM8 is a~~ [[~~Protein complex~~]] structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA].	+	[[3cm8]] is a 2 chain structure of [[Polymerase acidic protein]] with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA].
		+
		+	==See Also==
		+	*[[Polymerase acidic protein\|Polymerase acidic protein]]

	==Reference==		==Reference==
-	Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus., He X, Zhou J, Bartlam M, Zhang R, Ma J, Lou Z, Li X, Li J, Joachimiak A, Zeng Z, Ge R, Rao Z, Liu Y, Nature. 2008 Aug 28;454(7208):1123-6. Epub 2008 Jul 9. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~18615018 18615018]~~	+	<ref group="xtra">PMID:018615018</ref><references group="xtra"/>
	[[Category: Influenza a virus]]		[[Category: Influenza a virus]]
-	[[Category: Protein complex]]
	[[Category: RNA-directed RNA polymerase]]		[[Category: RNA-directed RNA polymerase]]
	[[Category: He, X.]]		[[Category: He, X.]]
Line 37:		Line 27:
	[[Category: Nucleotidyltransferase]]		[[Category: Nucleotidyltransferase]]
	[[Category: Protein-peptide complex]]		[[Category: Protein-peptide complex]]
-	[[Category: Rna binding protein/transferase complex]]	+	[[Category: Rna binding protein-transferase complex]]
	[[Category: Rna replication]]		[[Category: Rna replication]]
	[[Category: Rna-directed rna polymerase]]		[[Category: Rna-directed rna polymerase]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 3 10:36:54 2008''

OCA at 07:36, 3 September 2008

OCA — Wed, 03 Sep 2008 07:36:55 GMT

←Older revision		Revision as of 07:36, 3 September 2008
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3cm8.~~jpg~~\|left\|200px]]	+	[[Image:3cm8.png\|left\|200px]]

	<!--		<!--
Line 12:		Line 12:
	===A RNA polymerase subunit structure from virus===		===A RNA polymerase subunit structure from virus===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18615018}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18615018 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18615018}}

	==About this Structure==		==About this Structure==
	3CM8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA].		3CM8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM8 OCA].
		+
		+	==Reference==
		+	Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus., He X, Zhou J, Bartlam M, Zhang R, Ma J, Lou Z, Li X, Li J, Joachimiak A, Zeng Z, Ge R, Rao Z, Liu Y, Nature. 2008 Aug 28;454(7208):1123-6. Epub 2008 Jul 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18615018 18615018]
	[[Category: Influenza a virus]]		[[Category: Influenza a virus]]
	[[Category: Protein complex]]		[[Category: Protein complex]]
Line 33:		Line 42:
	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jul 16~~ 10:47:27 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 3 10:36:54 2008''