3cpo - Revision history

OCA at 12:31, 30 August 2023

OCA — Wed, 30 Aug 2023 12:31:52 GMT

←Older revision		Revision as of 12:31, 30 August 2023
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	<StructureSection load='3cpo' size='340' side='right'caption='[[3cpo]], [[Resolution\|resolution]] 1.24Å' scene=''>		<StructureSection load='3cpo' size='340' side='right'caption='[[3cpo]], [[Resolution\|resolution]] 1.24Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cpo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cpo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CPO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FP2:2~~-~~FLUOROPHENOL'>FP2</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.24Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ksi ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~303~~ "~~Bacillus fluorescens putidus~~" ~~Flugge 1886])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FP2:2-FLUOROPHENOL'>FP2</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cpo OCA], [https://pdbe.org/3cpo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cpo RCSB], [https://www.ebi.ac.uk/pdbsum/3cpo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cpo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cpo OCA], [https://pdbe.org/3cpo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cpo RCSB], [https://www.ebi.ac.uk/pdbsum/3cpo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cpo ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SDIS_PSEPU SDIS_PSEPU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus fluorescens putidus flugge 1886]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Steroid Delta-isomerase~~]]	+	[[Category: Pseudomonas putida]]
-	[[Category: Caaveiro~~, J M.M~~]]	+	[[Category: Caaveiro JMM]]
-	[[Category: Petsko, G]]	+	[[Category: Petsko G]]
-	[[Category: Pybus, B]]	+	[[Category: Pybus B]]
-	[[Category: Ringe, D]]	+	[[Category: Ringe D]]
-	~~[[Category: Active site]]~~	+
-	~~[[Category: Enzyme]]~~	+
-	~~[[Category: Geometry]]~~	+
-	~~[[Category: Hydrogen bond]]~~	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Lipid metabolism]]~~	+
-	~~[[Category: Steroid metabolism~~]]	+

OCA at 19:12, 20 October 2021

OCA — Wed, 20 Oct 2021 19:12:52 GMT

←Older revision		Revision as of 19:12, 20 October 2021
Line 1:		Line 1:

	==Crystal structure of ketosteroid isomerase D40N with bound 2-fluorophenol==		==Crystal structure of ketosteroid isomerase D40N with bound 2-fluorophenol==
-	<StructureSection load='3cpo' size='340' side='right' caption='[[3cpo]], [[Resolution\|resolution]] 1.24Å' scene=''>	+	<StructureSection load='3cpo' size='340' side='right'caption='[[3cpo]], [[Resolution\|resolution]] 1.24Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cpo]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cpo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CPO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FP2:2-FLUOROPHENOL'>FP2</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FP2:2-FLUOROPHENOL'>FP2</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3cpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cpo OCA], [~~http~~://pdbe.org/3cpo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3cpo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3cpo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3cpo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cpo OCA], [https://pdbe.org/3cpo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cpo RCSB], [https://www.ebi.ac.uk/pdbsum/3cpo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cpo ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cp/3cpo_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cp/3cpo_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 3cpo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3cpo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ketosteroid Isomerase\|Ketosteroid Isomerase]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus fluorescens putidus flugge 1886]]		[[Category: Bacillus fluorescens putidus flugge 1886]]
		+	[[Category: Large Structures]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]
	[[Category: Caaveiro, J M.M]]		[[Category: Caaveiro, J M.M]]

OCA at 08:32, 25 October 2017

OCA — Wed, 25 Oct 2017 08:32:41 GMT

←Older revision		Revision as of 08:32, 25 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of ketosteroid isomerase D40N with bound 2-fluorophenol==		==Crystal structure of ketosteroid isomerase D40N with bound 2-fluorophenol==
	<StructureSection load='3cpo' size='340' side='right' caption='[[3cpo]], [[Resolution\|resolution]] 1.24Å' scene=''>		<StructureSection load='3cpo' size='340' side='right' caption='[[3cpo]], [[Resolution\|resolution]] 1.24Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cpo OCA], [http://pdbe.org/3cpo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cpo RCSB], [http://www.ebi.ac.uk/pdbsum/3cpo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cpo OCA], [http://pdbe.org/3cpo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cpo RCSB], [http://www.ebi.ac.uk/pdbsum/3cpo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cpo ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 3cpo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3cpo" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Ketosteroid Isomerase\|Ketosteroid Isomerase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:12, 7 February 2016

OCA — Sun, 07 Feb 2016 08:12:27 GMT

←Older revision		Revision as of 08:12, 7 February 2016
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	<StructureSection load='3cpo' size='340' side='right' caption='[[3cpo]], [[Resolution\|resolution]] 1.24Å' scene=''>		<StructureSection load='3cpo' size='340' side='right' caption='[[3cpo]], [[Resolution\|resolution]] 1.24Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cpo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_putida Pseudomonas putida~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cpo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CPO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FP2:2-FLUOROPHENOL'>FP2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FP2:2-FLUOROPHENOL'>FP2</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 ~~Pseudomonas putida~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cpo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cpo RCSB], [http://www.ebi.ac.uk/pdbsum/3cpo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cpo OCA], [http://pdbe.org/3cpo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cpo RCSB], [http://www.ebi.ac.uk/pdbsum/3cpo PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cpo ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3cpo" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseudomonas putida~~]]	+	[[Category: Bacillus fluorescens putidus flugge 1886]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]
	[[Category: Caaveiro, J M.M]]		[[Category: Caaveiro, J M.M]]

OCA at 11:03, 20 January 2015

OCA — Tue, 20 Jan 2015 11:03:41 GMT

←Older revision		Revision as of 11:03, 20 January 2015
Line 35:		Line 35:
	[[Category: Pseudomonas putida]]		[[Category: Pseudomonas putida]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]
-	[[Category: Caaveiro, J M.M.]]	+	[[Category: Caaveiro, J M.M]]
-	[[Category: Petsko, G.]]	+	[[Category: Petsko, G]]
-	[[Category: Pybus, B.]]	+	[[Category: Pybus, B]]
-	[[Category: Ringe, D.]]	+	[[Category: Ringe, D]]
	[[Category: Active site]]		[[Category: Active site]]
	[[Category: Enzyme]]		[[Category: Enzyme]]

OCA at 06:23, 10 October 2014

OCA — Fri, 10 Oct 2014 06:23:05 GMT

←Older revision		Revision as of 06:23, 10 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3cpo\| PDB=3cpo \| SCENE= }}~~	+	==Crystal structure of ketosteroid isomerase D40N with bound 2-fluorophenol==
-	===Crystal structure of ketosteroid isomerase D40N with bound 2-fluorophenol===	+	<StructureSection load='3cpo' size='340' side='right' caption='[[3cpo]], [[Resolution\|resolution]] 1.24Å' scene=''>
-	~~{{ABSTRACT_PUBMED_18808119}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3cpo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CPO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FP2:2-FLUOROPHENOL'>FP2</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cpo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cpo RCSB], [http://www.ebi.ac.uk/pdbsum/3cpo PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cp/3cpo_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enzymes are classically proposed to accelerate reactions by binding substrates within active-site environments that are structurally preorganized to optimize binding interactions with reaction transition states rather than ground states. This is a remarkably formidable task considering the limited 0.1-1 A scale of most substrate rearrangements. The flexibility of active-site functional groups along the coordinate of substrate rearrangement, the distance scale on which enzymes can distinguish structural rearrangement, and the energetic significance of discrimination on that scale remain open questions that are fundamental to a basic physical understanding of enzyme active sites and catalysis. We bring together 1.2-1.5 A resolution X-ray crystallography, (1)H and (19)F NMR spectroscopy, quantum mechanical calculations, and transition-state analogue binding measurements to test the distance scale on which noncovalent forces can constrain the structural relaxation or translation of side chains and ligands along a specific coordinate and the energetic consequences of such geometric constraints within the active site of bacterial ketosteroid isomerase (KSI). Our results strongly suggest that packing and binding interactions within the KSI active site can constrain local side-chain reorientation and prevent hydrogen bond shortening by 0.1 A or less. Further, this constraint has substantial energetic effects on ligand binding and stabilization of negative charge within the oxyanion hole. These results provide evidence that subtle geometric effects, indistinguishable in most X-ray crystallographic structures, can have significant energetic consequences and highlight the importance of using synergistic experimental approaches to dissect enzyme function.

-	~~==About this Structure==~~	+	Testing geometrical discrimination within an enzyme active site: constrained hydrogen bonding in the ketosteroid isomerase oxyanion hole.,Sigala PA, Kraut DA, Caaveiro JM, Pybus B, Ruben EA, Ringe D, Petsko GA, Herschlag D J Am Chem Soc. 2008 Oct 15;130(41):13696-708. Epub 2008 Sep 23. PMID:18808119<ref>PMID:18808119</ref>
-	~~[[3cpo]] is a 1 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_putida Pseudomonas putida]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3CPO OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Ketosteroid Isomerase\|Ketosteroid Isomerase]]		*[[Ketosteroid Isomerase\|Ketosteroid Isomerase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018808119<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Pseudomonas putida]]		[[Category: Pseudomonas putida]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]

OCA at 10:46, 11 March 2013

OCA — Mon, 11 Mar 2013 10:46:42 GMT

←Older revision		Revision as of 10:46, 11 March 2013
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-	[[Image:3cpo.png\|left\|200px]]
-
	{{STRUCTURE_3cpo\| PDB=3cpo \| SCENE= }}		{{STRUCTURE_3cpo\| PDB=3cpo \| SCENE= }}
-
	===Crystal structure of ketosteroid isomerase D40N with bound 2-fluorophenol===		===Crystal structure of ketosteroid isomerase D40N with bound 2-fluorophenol===
-
	{{ABSTRACT_PUBMED_18808119}}		{{ABSTRACT_PUBMED_18808119}}

OCA: Protected "3cpo" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 19:54:15 GMT

Protected "3cpo" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:54, 7 January 2013

OCA at 19:54, 7 January 2013

OCA — Mon, 07 Jan 2013 19:54:12 GMT

←Older revision		Revision as of 19:54, 7 January 2013
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-	{{Seed}}
	[[Image:3cpo.png\|left\|200px]]		[[Image:3cpo.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3cpo", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3cpo\| PDB=3cpo \| SCENE= }}		{{STRUCTURE_3cpo\| PDB=3cpo \| SCENE= }}

	===Crystal structure of ketosteroid isomerase D40N with bound 2-fluorophenol===		===Crystal structure of ketosteroid isomerase D40N with bound 2-fluorophenol===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18808119}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18808119 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18808119}}		{{ABSTRACT_PUBMED_18808119}}

	==About this Structure==		==About this Structure==
-	~~3CPO~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPO OCA].	+	[[3cpo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPO OCA].
		+
		+	==See Also==
		+	*[[Ketosteroid Isomerase\|Ketosteroid Isomerase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18808119~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018808119</ref><references group="xtra"/>
	[[Category: Pseudomonas putida]]		[[Category: Pseudomonas putida]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]
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	[[Category: Ringe, D.]]		[[Category: Ringe, D.]]
	[[Category: Active site]]		[[Category: Active site]]
-	[[Category: Binding]]
	[[Category: Enzyme]]		[[Category: Enzyme]]
	[[Category: Geometry]]		[[Category: Geometry]]
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	[[Category: Lipid metabolism]]		[[Category: Lipid metabolism]]
	[[Category: Steroid metabolism]]		[[Category: Steroid metabolism]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:29:49 2009''

OCA at 10:29, 16 February 2009

OCA — Mon, 16 Feb 2009 10:29:50 GMT

←Older revision		Revision as of 10:29, 16 February 2009
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	==About this Structure==		==About this Structure==
-	3CPO is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPO OCA].	+	3CPO is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPO OCA].

	==Reference==		==Reference==
-	Testing geometrical discrimination within an enzyme active site: constrained hydrogen bonding in the ketosteroid isomerase oxyanion hole., Sigala PA, Kraut DA, Caaveiro JM, Pybus B, Ruben EA, Ringe D, Petsko GA, Herschlag D, J Am Chem Soc. 2008 Oct 15;130(41):13696-708. Epub 2008 Sep 23. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18808119 18808119]~~	+	<ref group="xtra">PMID:18808119</ref><references group="xtra"/>
	[[Category: Pseudomonas putida]]		[[Category: Pseudomonas putida]]
-	[[Category: Single protein]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]
	[[Category: Caaveiro, J M.M.]]		[[Category: Caaveiro, J M.M.]]
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	[[Category: Steroid metabolism]]		[[Category: Steroid metabolism]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Oct 15~~ 12:35:~~39 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:29:49 2009''