3cuz - Revision history

OCA at 09:38, 21 February 2024

OCA — Wed, 21 Feb 2024 09:38:37 GMT

←Older revision		Revision as of 09:38, 21 February 2024
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	<StructureSection load='3cuz' size='340' side='right'caption='[[3cuz]], [[Resolution\|resolution]] 1.04Å' scene=''>		<StructureSection load='3cuz' size='340' side='right'caption='[[3cuz]], [[Resolution\|resolution]] 1.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cuz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cuz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CUZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.04Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[1d8c\|1d8c]]~~, [[~~3cux~~\|~~3cux]], [[3cv1\|3cv1]], [[3cv2\|3cv2~~]]~~</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>aceB, mas ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cuz OCA], [https://pdbe.org/3cuz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cuz RCSB], [https://www.ebi.ac.uk/pdbsum/3cuz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cuz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cuz OCA], [https://pdbe.org/3cuz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cuz RCSB], [https://www.ebi.ac.uk/pdbsum/3cuz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cuz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MASY_ECOLI MASY_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cuz ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cuz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Enzymes of the glyoxylate shunt are important for the virulence of pathogenic organisms such as Mycobacterium tuberculosis and Candida albicans. Two isoforms have been identified for malate synthase, the second enzyme in the pathway. Isoform A, found in fungi and plants, comprises approximately 530 residues, whereas isoform G, found only in bacteria, is larger by approximately 200 residues. Crystal structures of malate synthase isoform G from Escherichia coli and Mycobacterium tuberculosis were previously determined at moderate resolution. Here we describe crystal structures of E. coli malate synthase A (MSA) in the apo form (1.04 A resolution) and in complex with acetyl-coenzyme A and a competitive inhibitor, possibly pyruvate or oxalate (1.40 A resolution). In addition, a crystal structure for Bacillus anthracis MSA at 1.70 A resolution is reported. The increase in size between isoforms A and G can be attributed primarily to an inserted alpha/beta domain that may have regulatory function. Upon binding of inhibitor or substrate, several active site loops in MSA undergo large conformational changes. However, in the substrate bound form, the active sites of isoforms A and G from E. coli are nearly identical. Considering that inhibitors bind with very similar affinities to both isoforms, MSA is as an excellent platform for high-resolution structural studies and drug discovery efforts.
-
-	Atomic resolution structures of Escherichia coli and Bacillus anthracis malate synthase A: comparison with isoform G and implications for structure-based drug discovery.,Lohman JR, Olson AC, Remington SJ Protein Sci. 2008 Nov;17(11):1935-45. Epub 2008 Aug 19. PMID:18714089<ref>PMID:18714089</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3cuz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Malate synthase 3D structures\|Malate synthase 3D structures]]		*[[Malate synthase 3D structures\|Malate synthase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Malate synthase]]~~	+	[[Category: Lohman JR]]
-	[[Category: Lohman~~, J R~~]]	+	[[Category: Remington SJ]]
-	[[Category: Remington~~, S J]]~~	+
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Glyoxylate bypass]]~~	+
-	~~[[Category: Tim barrel]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Tricarboxylic acid cycle~~]]	+

OCA at 07:46, 2 February 2022

OCA — Wed, 02 Feb 2022 07:46:07 GMT

←Older revision		Revision as of 07:46, 2 February 2022
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	==Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design==		==Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design==
-	<StructureSection load='3cuz' size='340' side='right' caption='[[3cuz]], [[Resolution\|resolution]] 1.04Å' scene=''>	+	<StructureSection load='3cuz' size='340' side='right'caption='[[3cuz]], [[Resolution\|resolution]] 1.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cuz]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cuz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CUZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8c\|1d8c]], [[3cux\|3cux]], [[3cv1\|3cv1]], [[3cv2\|3cv2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d8c\|1d8c]], [[3cux\|3cux]], [[3cv1\|3cv1]], [[3cv2\|3cv2]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB, mas ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB, mas ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3cuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cuz OCA], [~~http~~://pdbe.org/3cuz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3cuz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3cuz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3cuz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cuz OCA], [https://pdbe.org/3cuz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cuz RCSB], [https://www.ebi.ac.uk/pdbsum/3cuz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cuz ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Malate synthase\|Malate synthase]]	+	*[[Malate synthase 3D structures\|Malate synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]
	[[Category: Lohman, J R]]		[[Category: Lohman, J R]]

OCA at 13:48, 7 November 2018

OCA — Wed, 07 Nov 2018 13:48:56 GMT

←Older revision		Revision as of 13:48, 7 November 2018
Line 1:		Line 1:
		+
	==Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design==		==Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design==
	<StructureSection load='3cuz' size='340' side='right' caption='[[3cuz]], [[Resolution\|resolution]] 1.04Å' scene=''>		<StructureSection load='3cuz' size='340' side='right' caption='[[3cuz]], [[Resolution\|resolution]] 1.04Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB, mas ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB, mas ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cuz OCA], [http://pdbe.org/3cuz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cuz RCSB], [http://www.ebi.ac.uk/pdbsum/3cuz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cuz OCA], [http://pdbe.org/3cuz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cuz RCSB], [http://www.ebi.ac.uk/pdbsum/3cuz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cuz ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/3cuz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/3cuz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 39:		Line 40:
	[[Category: Lohman, J R]]		[[Category: Lohman, J R]]
	[[Category: Remington, S J]]		[[Category: Remington, S J]]
		+	[[Category: Cytoplasm]]
	[[Category: Glyoxylate bypass]]		[[Category: Glyoxylate bypass]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Tricarboxylic acid cycle]]		[[Category: Tricarboxylic acid cycle]]

OCA at 13:42, 9 February 2016

OCA — Tue, 09 Feb 2016 13:42:18 GMT

←Older revision		Revision as of 13:42, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3cuz' size='340' side='right' caption='[[3cuz]], [[Resolution\|resolution]] 1.04Å' scene=''>		<StructureSection load='3cuz' size='340' side='right' caption='[[3cuz]], [[Resolution\|resolution]] 1.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3cuz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3cuz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CUZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8c\|1d8c]], [[3cux\|3cux]], [[3cv1\|3cv1]], [[3cv2\|3cv2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8c\|1d8c]], [[3cux\|3cux]], [[3cv1\|3cv1]], [[3cv2\|3cv2]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB, mas ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB, mas ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cuz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cuz RCSB], [http://www.ebi.ac.uk/pdbsum/3cuz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cuz OCA], [http://pdbe.org/3cuz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cuz RCSB], [http://www.ebi.ac.uk/pdbsum/3cuz PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cuz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3cuz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]
	[[Category: Lohman, J R]]		[[Category: Lohman, J R]]

OCA at 08:23, 20 January 2015

OCA — Tue, 20 Jan 2015 08:23:28 GMT

←Older revision		Revision as of 08:23, 20 January 2015
Line 36:		Line 36:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]
-	[[Category: Lohman, J R.]]	+	[[Category: Lohman, J R]]
-	[[Category: Remington, S J.]]	+	[[Category: Remington, S J]]
	[[Category: Glyoxylate bypass]]		[[Category: Glyoxylate bypass]]
-	[[Category: Malate synthase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Tricarboxylic acid cycle]]		[[Category: Tricarboxylic acid cycle]]

OCA at 06:06, 10 October 2014

OCA — Fri, 10 Oct 2014 06:06:20 GMT

←Older revision		Revision as of 06:06, 10 October 2014
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-	[[~~Image:~~3cuz.~~png~~\|~~left~~\|200px]]	+	==Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design==
		+	<StructureSection load='3cuz' size='340' side='right' caption='[[3cuz]], [[Resolution\|resolution]] 1.04Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3cuz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CUZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8c\|1d8c]], [[3cux\|3cux]], [[3cv1\|3cv1]], [[3cv2\|3cv2]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aceB, mas ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cuz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cuz RCSB], [http://www.ebi.ac.uk/pdbsum/3cuz PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/3cuz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enzymes of the glyoxylate shunt are important for the virulence of pathogenic organisms such as Mycobacterium tuberculosis and Candida albicans. Two isoforms have been identified for malate synthase, the second enzyme in the pathway. Isoform A, found in fungi and plants, comprises approximately 530 residues, whereas isoform G, found only in bacteria, is larger by approximately 200 residues. Crystal structures of malate synthase isoform G from Escherichia coli and Mycobacterium tuberculosis were previously determined at moderate resolution. Here we describe crystal structures of E. coli malate synthase A (MSA) in the apo form (1.04 A resolution) and in complex with acetyl-coenzyme A and a competitive inhibitor, possibly pyruvate or oxalate (1.40 A resolution). In addition, a crystal structure for Bacillus anthracis MSA at 1.70 A resolution is reported. The increase in size between isoforms A and G can be attributed primarily to an inserted alpha/beta domain that may have regulatory function. Upon binding of inhibitor or substrate, several active site loops in MSA undergo large conformational changes. However, in the substrate bound form, the active sites of isoforms A and G from E. coli are nearly identical. Considering that inhibitors bind with very similar affinities to both isoforms, MSA is as an excellent platform for high-resolution structural studies and drug discovery efforts.

-	~~{{STRUCTURE_3cuz\| PDB=3cuz \| SCENE= }}~~	+	Atomic resolution structures of Escherichia coli and Bacillus anthracis malate synthase A: comparison with isoform G and implications for structure-based drug discovery.,Lohman JR, Olson AC, Remington SJ Protein Sci. 2008 Nov;17(11):1935-45. Epub 2008 Aug 19. PMID:18714089<ref>PMID:18714089</ref>

-	~~===Atomic Resolution Structures~~ of ~~Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_18714089}}~~	+	==See Also==
-		+	*[[Malate synthase\|Malate synthase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~3cuz~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUZ OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:018714089<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]

OCA: Protected "3cuz" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 18:13:24 GMT

Protected "3cuz" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:13, 7 January 2013

OCA at 18:13, 7 January 2013

OCA — Mon, 07 Jan 2013 18:13:22 GMT

←Older revision		Revision as of 18:13, 7 January 2013
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-	{{Seed}}
	[[Image:3cuz.png\|left\|200px]]		[[Image:3cuz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3cuz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3cuz\| PDB=3cuz \| SCENE= }}		{{STRUCTURE_3cuz\| PDB=3cuz \| SCENE= }}

	===Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design===		===Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18714089}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18714089 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18714089}}		{{ABSTRACT_PUBMED_18714089}}

	==About this Structure==		==About this Structure==
-	~~3CUZ~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUZ OCA].	+	[[3cuz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUZ OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18714089~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018714089</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]
	[[Category: Lohman, J R.]]		[[Category: Lohman, J R.]]
	[[Category: Remington, S J.]]		[[Category: Remington, S J.]]
-	[[Category: Cytoplasm]]
	[[Category: Glyoxylate bypass]]		[[Category: Glyoxylate bypass]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]
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	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Tricarboxylic acid cycle]]		[[Category: Tricarboxylic acid cycle]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:12:45 2009''

OCA at 08:12, 17 February 2009

OCA — Tue, 17 Feb 2009 08:12:47 GMT

←Older revision		Revision as of 08:12, 17 February 2009
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	{{Seed}}		{{Seed}}
-	[[Image:3cuz.~~jpg~~\|left\|200px]]	+	[[Image:3cuz.png\|left\|200px]]

	<!--		<!--
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	==Reference==		==Reference==
-	Atomic resolution structures of Escherichia coli and Bacillus anthracis malate synthase A: comparison with isoform G and implications for structure-based drug discovery., Lohman JR, Olson AC, Remington SJ, Protein Sci. 2008 Nov;17(11):1935-45. Epub 2008 Aug 19. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18714089 18714089]~~	+	<ref group="xtra">PMID:18714089</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Malate synthase]]		[[Category: Malate synthase]]
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	[[Category: Tricarboxylic acid cycle]]		[[Category: Tricarboxylic acid cycle]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Nov 12~~ 10:46:~~27 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:12:45 2009''

OCA at 08:46, 12 November 2008

OCA — Wed, 12 Nov 2008 08:46:28 GMT

←Older revision		Revision as of 08:46, 12 November 2008
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3cuz.jpg\|left\|200px]]

-	The ~~entry 3cuz~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3cuz", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3cuz\| PDB=3cuz \| SCENE= }}

-	~~Authors~~: ~~Lohman, J.R., Remington, S.J.~~	+	===Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design===

-	Description: Atomic Resolution Structures of Escherichia coli and Bacillis anthracis Malate Synthase A: Comparison with Isoform G and Implications for Structure Based Drug Design

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri Jul 11 13~~:04:15 2008''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18714089}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18714089 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18714089}}
		+
		+	==About this Structure==
		+	3CUZ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CUZ OCA].
		+
		+	==Reference==
		+	Atomic resolution structures of Escherichia coli and Bacillus anthracis malate synthase A: comparison with isoform G and implications for structure-based drug discovery., Lohman JR, Olson AC, Remington SJ, Protein Sci. 2008 Nov;17(11):1935-45. Epub 2008 Aug 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18714089 18714089]
		+	[[Category: Escherichia coli]]
		+	[[Category: Malate synthase]]
		+	[[Category: Lohman, J R.]]
		+	[[Category: Remington, S J.]]
		+	[[Category: Cytoplasm]]
		+	[[Category: Glyoxylate bypass]]
		+	[[Category: Malate synthase]]
		+	[[Category: Tim barrel]]
		+	[[Category: Transferase]]
		+	[[Category: Tricarboxylic acid cycle]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 12 10:46:27 2008''