3d0z - Revision history

OCA at 12:36, 30 August 2023

OCA — Wed, 30 Aug 2023 12:36:58 GMT

←Older revision		Revision as of 12:36, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='3d0z' size='340' side='right'caption='[[3d0z]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3d0z' size='340' side='right'caption='[[3d0z]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3d0z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Blood_fluke Blood fluke~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D0Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3d0z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_japonicum Schistosoma japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D0Z FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3crt\|3crt]], [[3cru\|3cru]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [https://pdbe.org/3d0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [https://www.ebi.ac.uk/pdbsum/3d0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d0z ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [https://pdbe.org/3d0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [https://www.ebi.ac.uk/pdbsum/3d0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d0z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GST26_SCHJA GST26_SCHJA]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.	+	[https://www.uniprot.org/uniprot/GST26_SCHJA GST26_SCHJA] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Blood fluke]]
-	[[Category: Glutathione transferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Sagermann, M~~]]	+	[[Category: Schistosoma japonicum]]
-	[[Category: ~~Engineered allostery]]~~	+	[[Category: Sagermann M]]
-	~~[[Category: Ph-switch]]~~	+
-	~~[[Category: Protein design]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 19:17, 20 October 2021

OCA — Wed, 20 Oct 2021 19:17:31 GMT

←Older revision		Revision as of 19:17, 20 October 2021
Line 3:		Line 3:
	<StructureSection load='3d0z' size='340' side='right'caption='[[3d0z]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3d0z' size='340' side='right'caption='[[3d0z]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3d0z]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3D0Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3d0z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D0Z FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3crt\|3crt]], [[3cru\|3cru]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3crt\|3crt]], [[3cru\|3cru]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [~~http~~://pdbe.org/3d0z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3d0z PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3d0z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [https://pdbe.org/3d0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [https://www.ebi.ac.uk/pdbsum/3d0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d0z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GST26_SCHJA GST26_SCHJA]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.	+	[[https://www.uniprot.org/uniprot/GST26_SCHJA GST26_SCHJA]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:55, 19 August 2020

OCA — Wed, 19 Aug 2020 06:55:46 GMT

←Older revision		Revision as of 06:55, 19 August 2020
Line 1:		Line 1:

	==Structural charcaterization of an engineered allosteric protein==		==Structural charcaterization of an engineered allosteric protein==
-	<StructureSection load='3d0z' size='340' side='right' caption='[[3d0z]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3d0z' size='340' side='right'caption='[[3d0z]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3d0z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3D0Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3d0z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3D0Z FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3crt\|3crt]], [[3cru\|3cru]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3crt\|3crt]], [[3cru\|3cru]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [http://pdbe.org/3d0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [http://www.ebi.ac.uk/pdbsum/3d0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3d0z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [http://pdbe.org/3d0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [http://www.ebi.ac.uk/pdbsum/3d0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3d0z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Glutathione S-transferase\|Glutathione S-transferase]]	+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 39:
	[[Category: Blood fluke]]		[[Category: Blood fluke]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
		+	[[Category: Large Structures]]
	[[Category: Sagermann, M]]		[[Category: Sagermann, M]]
	[[Category: Engineered allostery]]		[[Category: Engineered allostery]]

OCA at 09:54, 14 November 2018

OCA — Wed, 14 Nov 2018 09:54:09 GMT

←Older revision		Revision as of 09:54, 14 November 2018
Line 1:		Line 1:
		+
	==Structural charcaterization of an engineered allosteric protein==		==Structural charcaterization of an engineered allosteric protein==
	<StructureSection load='3d0z' size='340' side='right' caption='[[3d0z]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3d0z' size='340' side='right' caption='[[3d0z]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3crt\|3crt]], [[3cru\|3cru]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3crt\|3crt]], [[3cru\|3cru]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [http://pdbe.org/3d0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [http://www.ebi.ac.uk/pdbsum/3d0z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [http://pdbe.org/3d0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [http://www.ebi.ac.uk/pdbsum/3d0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3d0z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d0/3d0z_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d0/3d0z_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 09:37, 7 February 2016

OCA — Sun, 07 Feb 2016 09:37:20 GMT

←Older revision		Revision as of 09:37, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='3d0z' size='340' side='right' caption='[[3d0z]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3d0z' size='340' side='right' caption='[[3d0z]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3d0z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Schistosoma_japonicum Schistosoma japonicum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3D0Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3d0z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3D0Z FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3crt\|3crt]], [[3cru\|3cru]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3crt\|3crt]], [[3cru\|3cru]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [http://www.ebi.ac.uk/pdbsum/3d0z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [http://pdbe.org/3d0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [http://www.ebi.ac.uk/pdbsum/3d0z PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d0z ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3d0z" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Blood fluke]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
-	[[Category: Schistosoma japonicum]]
	[[Category: Sagermann, M]]		[[Category: Sagermann, M]]
	[[Category: Engineered allostery]]		[[Category: Engineered allostery]]

OCA at 14:07, 24 December 2014

OCA — Wed, 24 Dec 2014 14:07:41 GMT

←Older revision		Revision as of 14:07, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [http://www.ebi.ac.uk/pdbsum/3d0z PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [http://www.ebi.ac.uk/pdbsum/3d0z PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GST26_SCHJA GST26_SCHJA]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Schistosoma japonicum]]		[[Category: Schistosoma japonicum]]
-	[[Category: Sagermann, M.]]	+	[[Category: Sagermann, M]]
	[[Category: Engineered allostery]]		[[Category: Engineered allostery]]
	[[Category: Ph-switch]]		[[Category: Ph-switch]]
	[[Category: Protein design]]		[[Category: Protein design]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 09:28, 5 November 2014

OCA — Wed, 05 Nov 2014 09:28:18 GMT

←Older revision		Revision as of 09:28, 5 November 2014
Line 1:		Line 1:
-	[[~~Image:~~3d0z.~~png~~\|~~left~~\|200px]]	+	==Structural charcaterization of an engineered allosteric protein==
		+	<StructureSection load='3d0z' size='340' side='right' caption='[[3d0z]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3d0z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Schistosoma_japonicum Schistosoma japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3D0Z FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3crt\|3crt]], [[3cru\|3cru]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3d0z RCSB], [http://www.ebi.ac.uk/pdbsum/3d0z PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d0/3d0z_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Conformational changes play important roles in the regulation of many enzymatic reactions. Specific motions of side chains, secondary structures, or entire protein domains facilitate the precise control of substrate selection, binding, and catalysis. Likewise, the engineering of allostery into proteins is envisioned to enable unprecedented control of chemical reactions and molecular assembly processes. We here study the structural effects of engineered ionizable residues in the core of the glutathione-S-transferase to convert this protein into a pH-dependent allosteric protein. The underlying rational of these substitutions is that in the neutral state, an uncharged residue is compatible with the hydrophobic environment. In the charged state, however, the residue will invoke unfavorable interactions, which are likely to induce conformational changes that will affect the function of the enzyme. To test this hypothesis, we have engineered a single aspartate, cysteine, or histidine residue at a distance from the active site into the protein. All of the mutations exhibit a dramatic effect on the protein's affinity to bind glutathione. Whereas the aspartate or histidine mutations result in permanently nonbinding or binding versions of the protein, respectively, mutant GST50C exhibits distinct pH-dependent GSH-binding affinity. The crystal structures of the mutant protein GST50C under ionizing and nonionizing conditions reveal the recruitment of water molecules into the hydrophobic core to produce conformational changes that influence the protein's active site. The methodology described here to create and characterize engineered allosteric proteins through affinity chromatography may lead to a general approach to engineer effector-specific allostery into a protein structure.

-	~~{{STRUCTURE_3d0z\| PDB=3d0z \| SCENE= }}~~	+	Using affinity chromatography to engineer and characterize pH-dependent protein switches.,Sagermann M, Chapleau RR, DeLorimier E, Lei M Protein Sci. 2009 Jan;18(1):217-28. PMID:19177365<ref>PMID:19177365</ref>

-	~~===Structural charcaterization~~ of ~~an engineered allosteric protein===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19177365}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3d0z]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Schistosoma_japonicum Schistosoma japonicum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA].~~	+

	==See Also==		==See Also==
	*[[Glutathione S-transferase\|Glutathione S-transferase]]		*[[Glutathione S-transferase\|Glutathione S-transferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019177365<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Schistosoma japonicum]]		[[Category: Schistosoma japonicum]]

OCA: Protected "3d0z" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 20:25:33 GMT

Protected "3d0z" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:25, 7 January 2013

OCA at 20:25, 7 January 2013

OCA — Mon, 07 Jan 2013 20:25:29 GMT

←Older revision		Revision as of 20:25, 7 January 2013
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3d0z.png\|left\|200px]]
-	[[Image:3d0z.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3d0z", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3d0z\| PDB=3d0z \| SCENE= }}		{{STRUCTURE_3d0z\| PDB=3d0z \| SCENE= }}

	===Structural charcaterization of an engineered allosteric protein===		===Structural charcaterization of an engineered allosteric protein===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19177365}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19177365 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19177365}}		{{ABSTRACT_PUBMED_19177365}}

	==About this Structure==		==About this Structure==
-	~~3D0Z~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Schistosoma_japonicum Schistosoma japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA].	+	[[3d0z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Schistosoma_japonicum Schistosoma japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA].
		+
		+	==See Also==
		+	*[[Glutathione S-transferase\|Glutathione S-transferase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19177365~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019177365</ref><references group="xtra"/>
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Schistosoma japonicum]]		[[Category: Schistosoma japonicum]]
Line 31:		Line 22:
	[[Category: Protein design]]		[[Category: Protein design]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 25 09:28:57 2009''

OCA at 07:29, 25 February 2009

OCA — Wed, 25 Feb 2009 07:29:02 GMT

←Older revision		Revision as of 07:29, 25 February 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3d0z.jpg\|left\|200px]]

-	The ~~entry 3d0z~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3d0z", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3d0z\| PDB=3d0z \| SCENE= }}

-	~~Authors: Sagermann, M.~~	+	===Structural charcaterization of an engineered allosteric protein===

-	Description: Structural charcaterization of an engineered allosteric protein

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Oct 8~~ 09:16:~~46 2008~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19177365}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19177365 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19177365}}
		+
		+	==About this Structure==
		+	3D0Z is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Schistosoma_japonicum Schistosoma japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Z OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19177365</ref><references group="xtra"/>
		+	[[Category: Glutathione transferase]]
		+	[[Category: Schistosoma japonicum]]
		+	[[Category: Sagermann, M.]]
		+	[[Category: Engineered allostery]]
		+	[[Category: Ph-switch]]
		+	[[Category: Protein design]]
		+	[[Category: Transferase]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 25 09:28:57 2009''