3dhx - Revision history

OCA at 09:41, 21 February 2024

OCA — Wed, 21 Feb 2024 09:41:30 GMT

←Older revision		Revision as of 09:41, 21 February 2024
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	<StructureSection load='3dhx' size='340' side='right'caption='[[3dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3dhx' size='340' side='right'caption='[[3dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3dhx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DHX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3dhx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DHX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3dhw\|3dhw]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">metN, abc, b0199, JW0195 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhx OCA], [https://pdbe.org/3dhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dhx RCSB], [https://www.ebi.ac.uk/pdbsum/3dhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dhx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhx OCA], [https://pdbe.org/3dhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dhx RCSB], [https://www.ebi.ac.uk/pdbsum/3dhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dhx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/METN_ECOLI METN_ECOLI]] Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system (Probable). It has also been shown to be involved in formyl-L-methionine transport.<ref>PMID:12169620</ref> <ref>PMID:12819857</ref>	+	[https://www.uniprot.org/uniprot/METN_ECOLI METN_ECOLI] Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system (Probable). It has also been shown to be involved in formyl-L-methionine transport.<ref>PMID:12169620</ref> <ref>PMID:12819857</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dhx ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dhx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structure of the high-affinity Escherichia coli MetNI methionine uptake transporter, a member of the adenosine triphosphate (ATP)-binding cassette (ABC) family, has been solved to 3.7 angstrom resolution. The overall architecture of MetNI reveals two copies of the adenosine triphosphatase (ATPase) MetN in complex with two copies of the transmembrane domain MetI, with the transporter adopting an inward-facing conformation exhibiting widely separated nucleotide binding domains. Each MetI subunit is organized around a core of five transmembrane helices that correspond to a subset of the helices observed in the larger membrane-spanning subunits of the molybdate (ModBC) and maltose (MalFGK) ABC transporters. In addition to the conserved nucleotide binding domain of the ABC family, MetN contains a carboxyl-terminal extension with a ferredoxin-like fold previously assigned to a conserved family of regulatory ligand-binding domains. These domains separate the nucleotide binding domains and would interfere with their association required for ATP binding and hydrolysis. Methionine binds to the dimerized carboxyl-terminal domain and is shown to inhibit ATPase activity. These observations are consistent with an allosteric regulatory mechanism operating at the level of transport activity, where increased intracellular levels of the transported ligand stabilize an inward-facing, ATPase-inactive state of MetNI to inhibit further ligand translocation into the cell.
-
-	The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation.,Kadaba NS, Kaiser JT, Johnson E, Lee A, Rees DC Science. 2008 Jul 11;321(5886):250-3. PMID:18621668<ref>PMID:18621668</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3dhx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Johnson, E]]	+	[[Category: Johnson E]]
-	[[Category: Kaiser~~, J T~~]]	+	[[Category: Kaiser JT]]
-	[[Category: Lee~~, A T~~]]	+	[[Category: Lee AT]]
-	[[Category: Rees~~, D C]]~~	+	[[Category: Rees DC]]
-	~~[[Category: Amino-acid transport]]~~	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Inner membrane]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Methionine uptake]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Regulation]]~~	+
-	~~[[Category: Transport~~]]	+

OCA at 07:59, 9 February 2022

OCA — Wed, 09 Feb 2022 07:59:25 GMT

←Older revision		Revision as of 07:59, 9 February 2022
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	==Crystal structure of isolated C2 domain of the methionine uptake transporter==		==Crystal structure of isolated C2 domain of the methionine uptake transporter==
-	<StructureSection load='3dhx' size='340' side='right' caption='[[3dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3dhx' size='340' side='right'caption='[[3dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3dhx]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3DHX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3dhx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DHX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dhw\|3dhw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3dhw\|3dhw]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metN, abc, b0199, JW0195 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metN, abc, b0199, JW0195 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhx OCA], [~~http~~://pdbe.org/3dhx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3dhx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3dhx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3dhx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhx OCA], [https://pdbe.org/3dhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dhx RCSB], [https://www.ebi.ac.uk/pdbsum/3dhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dhx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/METN_ECOLI METN_ECOLI]] Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system (Probable). It has also been shown to be involved in formyl-L-methionine transport.<ref>PMID:12169620</ref> <ref>PMID:12819857</ref>	+	[[https://www.uniprot.org/uniprot/METN_ECOLI METN_ECOLI]] Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system (Probable). It has also been shown to be involved in formyl-L-methionine transport.<ref>PMID:12169620</ref> <ref>PMID:12819857</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[ABC transporter\|ABC transporter]]	+	*[[ABC transporter 3D structures\|ABC transporter 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Johnson, E]]		[[Category: Johnson, E]]
	[[Category: Kaiser, J T]]		[[Category: Kaiser, J T]]

OCA at 09:33, 14 November 2018

OCA — Wed, 14 Nov 2018 09:33:34 GMT

←Older revision		Revision as of 09:33, 14 November 2018
Line 1:		Line 1:
		+
	==Crystal structure of isolated C2 domain of the methionine uptake transporter==		==Crystal structure of isolated C2 domain of the methionine uptake transporter==
	<StructureSection load='3dhx' size='340' side='right' caption='[[3dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3dhx' size='340' side='right' caption='[[3dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dhw\|3dhw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dhw\|3dhw]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metN, abc, b0199, JW0195 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metN, abc, b0199, JW0195 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhx OCA], [http://pdbe.org/3dhx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dhx RCSB], [http://www.ebi.ac.uk/pdbsum/3dhx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhx OCA], [http://pdbe.org/3dhx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dhx RCSB], [http://www.ebi.ac.uk/pdbsum/3dhx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3dhx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dh/3dhx_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dh/3dhx_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 18:23, 8 February 2016

OCA — Mon, 08 Feb 2016 18:23:20 GMT

←Older revision		Revision as of 18:23, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='3dhx' size='340' side='right' caption='[[3dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3dhx' size='340' side='right' caption='[[3dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3dhx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DHX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3dhx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DHX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dhw\|3dhw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dhw\|3dhw]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metN, abc, b0199, JW0195 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metN, abc, b0199, JW0195 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dhx RCSB], [http://www.ebi.ac.uk/pdbsum/3dhx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhx OCA], [http://pdbe.org/3dhx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dhx RCSB], [http://www.ebi.ac.uk/pdbsum/3dhx PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dhx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3dhx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Ecoli]]
	[[Category: Johnson, E]]		[[Category: Johnson, E]]
	[[Category: Kaiser, J T]]		[[Category: Kaiser, J T]]

OCA at 01:16, 25 December 2014

OCA — Thu, 25 Dec 2014 01:16:53 GMT

←Older revision		Revision as of 01:16, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3dhx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DHX FirstGlance]. <br>		<table><tr><td colspan='2'>[[3dhx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DHX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dhw\|3dhw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dhw\|3dhw]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metN, abc, b0199, JW0195 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metN, abc, b0199, JW0195 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dhx RCSB], [http://www.ebi.ac.uk/pdbsum/3dhx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dhx RCSB], [http://www.ebi.ac.uk/pdbsum/3dhx PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/METN_ECOLI METN_ECOLI]] Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system (Probable). It has also been shown to be involved in formyl-L-methionine transport.<ref>PMID:12169620</ref> <ref>PMID:12819857</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Johnson, E.]]	+	[[Category: Johnson, E]]
-	[[Category: Kaiser, J T.]]	+	[[Category: Kaiser, J T]]
-	[[Category: Lee, A T.]]	+	[[Category: Lee, A T]]
-	[[Category: Rees, D C.]]	+	[[Category: Rees, D C]]
	[[Category: Amino-acid transport]]		[[Category: Amino-acid transport]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]

OCA at 13:06, 29 September 2014

OCA — Mon, 29 Sep 2014 13:06:02 GMT

←Older revision		Revision as of 13:06, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3dhx.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of isolated C2 domain of the methionine uptake transporter==
		+	<StructureSection load='3dhx' size='340' side='right' caption='[[3dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3dhx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DHX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dhw\|3dhw]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metN, abc, b0199, JW0195 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dhx RCSB], [http://www.ebi.ac.uk/pdbsum/3dhx PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dh/3dhx_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the high-affinity Escherichia coli MetNI methionine uptake transporter, a member of the adenosine triphosphate (ATP)-binding cassette (ABC) family, has been solved to 3.7 angstrom resolution. The overall architecture of MetNI reveals two copies of the adenosine triphosphatase (ATPase) MetN in complex with two copies of the transmembrane domain MetI, with the transporter adopting an inward-facing conformation exhibiting widely separated nucleotide binding domains. Each MetI subunit is organized around a core of five transmembrane helices that correspond to a subset of the helices observed in the larger membrane-spanning subunits of the molybdate (ModBC) and maltose (MalFGK) ABC transporters. In addition to the conserved nucleotide binding domain of the ABC family, MetN contains a carboxyl-terminal extension with a ferredoxin-like fold previously assigned to a conserved family of regulatory ligand-binding domains. These domains separate the nucleotide binding domains and would interfere with their association required for ATP binding and hydrolysis. Methionine binds to the dimerized carboxyl-terminal domain and is shown to inhibit ATPase activity. These observations are consistent with an allosteric regulatory mechanism operating at the level of transport activity, where increased intracellular levels of the transported ligand stabilize an inward-facing, ATPase-inactive state of MetNI to inhibit further ligand translocation into the cell.

-	~~{{STRUCTURE_3dhx\| PDB=3dhx \| SCENE= }}~~	+	The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation.,Kadaba NS, Kaiser JT, Johnson E, Lee A, Rees DC Science. 2008 Jul 11;321(5886):250-3. PMID:18621668<ref>PMID:18621668</ref>

-	~~===Crystal structure of isolated C2 domain~~ of the ~~methionine uptake transporter===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18621668}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3dhx]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA].~~	+

	==See Also==		==See Also==
	*[[ABC transporter\|ABC transporter]]		*[[ABC transporter\|ABC transporter]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018621668<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Johnson, E.]]		[[Category: Johnson, E.]]

OCA at 11:03, 20 October 2012

OCA — Sat, 20 Oct 2012 11:03:24 GMT

←Older revision		Revision as of 11:03, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3dhx]] is a 2 chain structure ~~of [[ABC transporter]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA].	+	[[3dhx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA].

	==See Also==		==See Also==

OCA: Protected "3dhx" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 04:06:31 GMT

Protected "3dhx" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:06, 26 July 2012

OCA at 04:06, 26 July 2012

OCA — Thu, 26 Jul 2012 04:06:29 GMT

←Older revision		Revision as of 04:06, 26 July 2012
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-	{{Seed}}
	[[Image:3dhx.png\|left\|200px]]		[[Image:3dhx.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3dhx", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_3dhx\| PDB=3dhx \| SCENE= }}		{{STRUCTURE_3dhx\| PDB=3dhx \| SCENE= }}

	===Crystal structure of isolated C2 domain of the methionine uptake transporter===		===Crystal structure of isolated C2 domain of the methionine uptake transporter===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18621668}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18621668 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_18621668}}		{{ABSTRACT_PUBMED_18621668}}

	==About this Structure==		==About this Structure==
-	~~3DHX~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k12~~ Escherichia coli ~~k12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA].	+	[[3dhx]] is a 2 chain structure of [[ABC transporter]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA].
		+
		+	==See Also==
		+	*[[ABC transporter\|ABC transporter]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18621668~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018621668</ref><references group="xtra"/>
-	[[Category: Escherichia coli ~~k12~~]]	+	[[Category: Escherichia coli]]
	[[Category: Johnson, E.]]		[[Category: Johnson, E.]]
	[[Category: Kaiser, J T.]]		[[Category: Kaiser, J T.]]
Line 38:		Line 29:
	[[Category: Regulation]]		[[Category: Regulation]]
	[[Category: Transport]]		[[Category: Transport]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 01:20:19 2009''

OCA at 23:20, 16 February 2009

OCA — Mon, 16 Feb 2009 23:20:21 GMT

←Older revision		Revision as of 23:20, 16 February 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3dhx.~~jpg~~\|left\|200px]]	+	[[Image:3dhx.png\|left\|200px]]

	<!--		<!--
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3DHX is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA].	+	3DHX is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHX OCA].

	==Reference==		==Reference==
-	~~The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation., Kadaba NS, Kaiser JT, Johnson E, Lee A, Rees DC, Science. 2008 Jul 11;321(5886):250-3.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18621668 18621668]~~	+	<ref group="xtra">PMID:18621668</ref><references group="xtra"/>
	[[Category: Escherichia coli k12]]		[[Category: Escherichia coli k12]]
-	[[Category: Single protein]]
	[[Category: Johnson, E.]]		[[Category: Johnson, E.]]
	[[Category: Kaiser, J T.]]		[[Category: Kaiser, J T.]]
Line 40:		Line 39:
	[[Category: Transport]]		[[Category: Transport]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Aug 6 13~~:05:19 ~~2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 01:20:19 2009''