3e1q - Revision history

OCA at 12:56, 30 August 2023

OCA — Wed, 30 Aug 2023 12:56:51 GMT

←Older revision		Revision as of 12:56, 30 August 2023
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	<StructureSection load='3e1q' size='340' side='right'caption='[[3e1q]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3e1q' size='340' side='right'caption='[[3e1q]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3e1q]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E1Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3e1q]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E1Q FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>~~, ~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3e1j\|3e1j]], [[3e1l\|3e1l]], [[3e1m\|3e1m]], [[3e1n\|3e1n]], [[3e1o\|3e1o]], [[3e1p\|3e1p]]~~</~~div~~><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">BFR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1q OCA], [https://pdbe.org/3e1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e1q RCSB], [https://www.ebi.ac.uk/pdbsum/3e1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1q OCA], [https://pdbe.org/3e1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e1q RCSB], [https://www.ebi.ac.uk/pdbsum/3e1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BFR_ECOLI BFR_ECOLI]] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex. The mineralized iron core can contain as many as 2700 iron atoms/24-meric molecule.<ref>PMID:10769150</ref> <ref>PMID:14636073</ref>	+	[https://www.uniprot.org/uniprot/BFR_ECOLI BFR_ECOLI] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex. The mineralized iron core can contain as many as 2700 iron atoms/24-meric molecule.<ref>PMID:10769150</ref> <ref>PMID:14636073</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Brun, N E.Le~~]]	+	[[Category: Crow A]]
-	[[Category: ~~Crow, A~~]]	+	[[Category: Lawson TL]]
-	[[Category: ~~Lawson, T L~~]]	+	[[Category: Le Brun NE]]
-	[[Category: Lewin, A]]	+	[[Category: Lewin A]]
-	[[Category: Moore~~, G R]]~~	+	[[Category: Moore GR]]
-	~~[[Category: Heme]]~~	+
-	~~[[Category: Iron]]~~	+
-	~~[[Category: Iron storage]]~~	+
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: W133f bacterioferritin. rhombic dodecahedral superstructure~~]]	+

OCA at 19:33, 20 October 2021

OCA — Wed, 20 Oct 2021 19:33:06 GMT

←Older revision		Revision as of 19:33, 20 October 2021
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	==Crystal structure of W133F variant E. coli Bacterioferritn with iron.==		==Crystal structure of W133F variant E. coli Bacterioferritn with iron.==
-	<StructureSection load='3e1q' size='340' side='right' caption='[[3e1q]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='3e1q' size='340' side='right'caption='[[3e1q]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3e1q]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3E1Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3e1q]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E1Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3e1j\|3e1j]], [[3e1l\|3e1l]], [[3e1m\|3e1m]], [[3e1n\|3e1n]], [[3e1o\|3e1o]], [[3e1p\|3e1p]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3e1j\|3e1j]], [[3e1l\|3e1l]], [[3e1m\|3e1m]], [[3e1n\|3e1n]], [[3e1o\|3e1o]], [[3e1p\|3e1p]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BFR ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BFR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1q OCA], [~~http~~://pdbe.org/3e1q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3e1q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3e1q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1q OCA], [https://pdbe.org/3e1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e1q RCSB], [https://www.ebi.ac.uk/pdbsum/3e1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BFR_ECOLI BFR_ECOLI]] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex. The mineralized iron core can contain as many as 2700 iron atoms/24-meric molecule.<ref>PMID:10769150</ref> <ref>PMID:14636073</ref>	+	[[https://www.uniprot.org/uniprot/BFR_ECOLI BFR_ECOLI]] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex. The mineralized iron core can contain as many as 2700 iron atoms/24-meric molecule.<ref>PMID:10769150</ref> <ref>PMID:14636073</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
-	*[[Ferritin\|Ferritin]]	+	*[[Ferritin 3D structures\|Ferritin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Brun, N E.Le]]		[[Category: Brun, N E.Le]]
	[[Category: Crow, A]]		[[Category: Crow, A]]

OCA at 09:58, 14 November 2018

OCA — Wed, 14 Nov 2018 09:58:55 GMT

←Older revision		Revision as of 09:58, 14 November 2018
Line 1:		Line 1:
		+
	==Crystal structure of W133F variant E. coli Bacterioferritn with iron.==		==Crystal structure of W133F variant E. coli Bacterioferritn with iron.==
	<StructureSection load='3e1q' size='340' side='right' caption='[[3e1q]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3e1q' size='340' side='right' caption='[[3e1q]], [[Resolution\|resolution]] 2.60Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3e1j\|3e1j]], [[3e1l\|3e1l]], [[3e1m\|3e1m]], [[3e1n\|3e1n]], [[3e1o\|3e1o]], [[3e1p\|3e1p]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3e1j\|3e1j]], [[3e1l\|3e1l]], [[3e1m\|3e1m]], [[3e1n\|3e1n]], [[3e1o\|3e1o]], [[3e1p\|3e1p]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BFR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BFR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1q OCA], [http://pdbe.org/3e1q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3e1q RCSB], [http://www.ebi.ac.uk/pdbsum/3e1q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1q OCA], [http://pdbe.org/3e1q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3e1q RCSB], [http://www.ebi.ac.uk/pdbsum/3e1q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/3e1q_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/3e1q_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 19:31, 9 February 2016

OCA — Tue, 09 Feb 2016 19:31:42 GMT

←Older revision		Revision as of 19:31, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3e1q' size='340' side='right' caption='[[3e1q]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3e1q' size='340' side='right' caption='[[3e1q]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3e1q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E1Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3e1q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E1Q FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3e1j\|3e1j]], [[3e1l\|3e1l]], [[3e1m\|3e1m]], [[3e1n\|3e1n]], [[3e1o\|3e1o]], [[3e1p\|3e1p]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3e1j\|3e1j]], [[3e1l\|3e1l]], [[3e1m\|3e1m]], [[3e1n\|3e1n]], [[3e1o\|3e1o]], [[3e1p\|3e1p]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BFR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BFR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3e1q RCSB], [http://www.ebi.ac.uk/pdbsum/3e1q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1q OCA], [http://pdbe.org/3e1q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3e1q RCSB], [http://www.ebi.ac.uk/pdbsum/3e1q PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e1q ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3e1q" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Ecoli]]
	[[Category: Brun, N E.Le]]		[[Category: Brun, N E.Le]]
	[[Category: Crow, A]]		[[Category: Crow, A]]

OCA at 09:03, 25 December 2014

OCA — Thu, 25 Dec 2014 09:03:13 GMT

←Older revision		Revision as of 09:03, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3e1q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E1Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[3e1q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E1Q FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3e1j\|3e1j]], [[3e1l\|3e1l]], [[3e1m\|3e1m]], [[3e1n\|3e1n]], [[3e1o\|3e1o]], [[3e1p\|3e1p]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3e1j\|3e1j]], [[3e1l\|3e1l]], [[3e1m\|3e1m]], [[3e1n\|3e1n]], [[3e1o\|3e1o]], [[3e1p\|3e1p]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BFR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BFR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3e1q RCSB], [http://www.ebi.ac.uk/pdbsum/3e1q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3e1q RCSB], [http://www.ebi.ac.uk/pdbsum/3e1q PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BFR_ECOLI BFR_ECOLI]] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex. The mineralized iron core can contain as many as 2700 iron atoms/24-meric molecule.<ref>PMID:10769150</ref> <ref>PMID:14636073</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Brun, N E.Le.]]	+	[[Category: Brun, N E.Le]]
-	[[Category: Crow, A.]]	+	[[Category: Crow, A]]
-	[[Category: Lawson, T L.]]	+	[[Category: Lawson, T L]]
-	[[Category: Lewin, A.]]	+	[[Category: Lewin, A]]
-	[[Category: Moore, G R.]]	+	[[Category: Moore, G R]]
	[[Category: Heme]]		[[Category: Heme]]
	[[Category: Iron]]		[[Category: Iron]]

OCA at 13:35, 29 September 2014

OCA — Mon, 29 Sep 2014 13:35:28 GMT

←Older revision		Revision as of 13:35, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3e1q.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of W133F variant E. coli Bacterioferritn with iron.==
		+	<StructureSection load='3e1q' size='340' side='right' caption='[[3e1q]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3e1q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E1Q FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3e1j\|3e1j]], [[3e1l\|3e1l]], [[3e1m\|3e1m]], [[3e1n\|3e1n]], [[3e1o\|3e1o]], [[3e1p\|3e1p]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BFR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3e1q RCSB], [http://www.ebi.ac.uk/pdbsum/3e1q PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/3e1q_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ferritins solubilize and detoxify the essential metal iron through formation of a ferric mineral within the protein's central cavity. Key to this activity is an intrasubunit catalytic dinuclear iron center called the ferroxidase center. Here we show that the fluorescence intensity of Escherichia coli bacterioferritin (BFR), due to the presence of two tryptophan residues (Trp35 and Trp133) in each of the 24 subunits, is highly sensitive to the iron status of the ferroxidase center and is quenched to different extents by Fe2+ and Fe3+. Recovery of the quench following oxidation of Fe2+ to Fe3+ at the ferroxidase center was not observed, indicating that the di-Fe3+ form of the center is stable. Studies of the single-tryptophan variants W35F and W133F showed that Trp133, which lies approximately 10 A from the ferroxidase center, is primarily responsible for the observed fluorescence sensitivity to iron, while studies of a stable E. coli BFR subunit dimer demonstrated that the observed quench properties are principally derived from the interaction of iron with tryptophan residues within the subunit dimer. A double-tryptophan variant (W35F/W133F) was found to exhibit fluorescence from the seven tyrosine residues present in each subunit, which was also sensitive to the iron status of the ferroxidase center. Finally, we demonstrate using Zn2+, a potent competitive inhibitor of Fe2+ binding and oxidation, that the fluorescence response can be used to monitor the loss of iron from the ferroxidase center.

-	~~{{STRUCTURE_3e1q\| PDB=3e1q \| SCENE= }}~~	+	Monitoring the iron status of the ferroxidase center of Escherichia coli bacterioferritin using fluorescence spectroscopy.,Lawson TL, Crow A, Lewin A, Yasmin S, Moore GR, Le Brun NE Biochemistry. 2009 Sep 29;48(38):9031-9. PMID:19705876<ref>PMID:19705876</ref>

-	~~===Crystal structure~~ of ~~W133F variant E~~. ~~coli Bacterioferritn with iron~~.~~===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19705876}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3e1q]] is a 12 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA].~~	+

	==See Also==		==See Also==
	*[[Ferritin\|Ferritin]]		*[[Ferritin\|Ferritin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019705876<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Brun, N E.Le.]]		[[Category: Brun, N E.Le.]]

OCA at 14:54, 20 October 2012

OCA — Sat, 20 Oct 2012 14:54:15 GMT

←Older revision		Revision as of 14:54, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3e1q]] is a 12 chain structure ~~of [[Ferritin]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA].	+	[[3e1q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA].

	==See Also==		==See Also==

OCA at 16:38, 26 July 2012

OCA — Thu, 26 Jul 2012 16:38:19 GMT

←Older revision		Revision as of 16:38, 26 July 2012
Line 1:		Line 1:
	[[Image:3e1q.png\|left\|200px]]		[[Image:3e1q.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3e1q", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3e1q\| PDB=3e1q \| SCENE= }}		{{STRUCTURE_3e1q\| PDB=3e1q \| SCENE= }}

	===Crystal structure of W133F variant E. coli Bacterioferritn with iron.===		===Crystal structure of W133F variant E. coli Bacterioferritn with iron.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19705876}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19705876 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19705876}}		{{ABSTRACT_PUBMED_19705876}}

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	==See Also==		==See Also==
-	*[[Ferritin]]	+	*[[Ferritin\|Ferritin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19705876~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019705876</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Brun, N E.Le.]]		[[Category: Brun, N E.Le.]]

OCA: Protected "3e1q" [edit=sysop:move=sysop]

OCA — Sun, 26 Dec 2010 23:23:41 GMT

Protected "3e1q" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:23, 26 December 2010

OCA at 23:23, 26 December 2010

OCA — Sun, 26 Dec 2010 23:23:40 GMT

←Older revision		Revision as of 23:23, 26 December 2010
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3e1q.png\|left\|200px]]		[[Image:3e1q.png\|left\|200px]]

Line 20:		Line 19:

	==About this Structure==		==About this Structure==
-	~~3E1Q~~ is a 12 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA].	+	[[3e1q]] is a 12 chain structure of [[Ferritin]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Q OCA].
		+
		+	==See Also==
		+	*[[Ferritin]]

	==Reference==		==Reference==
Line 36:		Line 38:
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
	[[Category: W133f bacterioferritin. rhombic dodecahedral superstructure]]		[[Category: W133f bacterioferritin. rhombic dodecahedral superstructure]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 14 09:49:33 2009''