3eak - Revision history

OCA at 15:20, 1 November 2023

OCA — Wed, 01 Nov 2023 15:20:26 GMT

←Older revision		Revision as of 15:20, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3eak' size='340' side='right'caption='[[3eak]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3eak' size='340' side='right'caption='[[3eak]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3eak]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arabian_camel Arabian camel~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3EAK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3eak]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EAK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3eak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eak OCA], [~~http~~://pdbe.org/3eak PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3eak RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3eak PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3eak ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eak OCA], [https://pdbe.org/3eak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eak RCSB], [https://www.ebi.ac.uk/pdbsum/3eak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eak ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arabian camel~~]]	+	[[Category: Camelus dromedarius]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Conrath, K]]	+	[[Category: Conrath K]]
-	[[Category: Loris, R]]	+	[[Category: Loris R]]
-	[[Category: Martinez-Rodriguez, S]]	+	[[Category: Martinez-Rodriguez S]]
-	[[Category: Muyldermans, S]]	+	[[Category: Muyldermans S]]
-	[[Category: Saerens, D]]	+	[[Category: Saerens D]]
-	[[Category: Vincke, C]]	+	[[Category: Vincke C]]
-	~~[[Category: Antibody]]~~	+
-	~~[[Category: Humanization]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Nanobody]]~~	+
-	~~[[Category: Vhh domain~~]]	+

OCA at 10:21, 9 September 2020

OCA — Wed, 09 Sep 2020 10:21:35 GMT

←Older revision		Revision as of 10:21, 9 September 2020
Line 1:		Line 1:

	==NbBCII10 humanized (FGLA mutant)==		==NbBCII10 humanized (FGLA mutant)==
-	<StructureSection load='3eak' size='340' side='right' caption='[[3eak]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='3eak' size='340' side='right'caption='[[3eak]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3eak]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3EAK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3eak]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3EAK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3eak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eak OCA], [http://pdbe.org/3eak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eak RCSB], [http://www.ebi.ac.uk/pdbsum/3eak PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eak ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3eak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eak OCA], [http://pdbe.org/3eak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eak RCSB], [http://www.ebi.ac.uk/pdbsum/3eak PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eak ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 29:		Line 29:
	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Arabian camel]]		[[Category: Arabian camel]]
		+	[[Category: Large Structures]]
	[[Category: Conrath, K]]		[[Category: Conrath, K]]
	[[Category: Loris, R]]		[[Category: Loris, R]]

OCA at 21:49, 2 December 2018

OCA — Sun, 02 Dec 2018 21:49:09 GMT

←Older revision		Revision as of 21:49, 2 December 2018
Line 1:		Line 1:
		+
	==NbBCII10 humanized (FGLA mutant)==		==NbBCII10 humanized (FGLA mutant)==
	<StructureSection load='3eak' size='340' side='right' caption='[[3eak]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3eak' size='340' side='right' caption='[[3eak]], [[Resolution\|resolution]] 1.95Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3eak]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EAK FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eak]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EAK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eak OCA], [http://pdbe.org/3eak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eak RCSB], [http://www.ebi.ac.uk/pdbsum/3eak PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eak OCA], [http://pdbe.org/3eak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eak RCSB], [http://www.ebi.ac.uk/pdbsum/3eak PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eak ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/3eak_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/3eak_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 25:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 3eak" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3eak" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:54, 9 February 2016

OCA — Tue, 09 Feb 2016 02:54:03 GMT

←Older revision		Revision as of 02:54, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3eak' size='340' side='right' caption='[[3eak]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3eak' size='340' side='right' caption='[[3eak]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3eak]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Camelus_dromedarius Camelus dromedarius~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EAK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3eak]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EAK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eak RCSB], [http://www.ebi.ac.uk/pdbsum/3eak PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eak OCA], [http://pdbe.org/3eak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eak RCSB], [http://www.ebi.ac.uk/pdbsum/3eak PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3eak ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3eak" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camelus dromedarius~~]]	+	[[Category: Arabian camel]]
	[[Category: Conrath, K]]		[[Category: Conrath, K]]
	[[Category: Loris, R]]		[[Category: Loris, R]]

OCA at 21:41, 3 January 2015

OCA — Sat, 03 Jan 2015 21:41:43 GMT

←Older revision		Revision as of 21:41, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3eak]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EAK FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eak]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EAK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eak RCSB], [http://www.ebi.ac.uk/pdbsum/3eak PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eak RCSB], [http://www.ebi.ac.uk/pdbsum/3eak PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 22:		Line 22:
	General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold.,Vincke C, Loris R, Saerens D, Martinez-Rodriguez S, Muyldermans S, Conrath K J Biol Chem. 2009 Jan 30;284(5):3273-84. Epub 2008 Nov 14. PMID:19010777<ref>PMID:19010777</ref>		General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold.,Vincke C, Loris R, Saerens D, Martinez-Rodriguez S, Muyldermans S, Conrath K J Biol Chem. 2009 Jan 30;284(5):3273-84. Epub 2008 Nov 14. PMID:19010777<ref>PMID:19010777</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Camelus dromedarius]]		[[Category: Camelus dromedarius]]
-	[[Category: Conrath, K.]]	+	[[Category: Conrath, K]]
-	[[Category: Loris, R.]]	+	[[Category: Loris, R]]
-	[[Category: Martinez-Rodriguez, S.]]	+	[[Category: Martinez-Rodriguez, S]]
-	[[Category: Muyldermans, S.]]	+	[[Category: Muyldermans, S]]
-	[[Category: Saerens, D.]]	+	[[Category: Saerens, D]]
-	[[Category: Vincke, C.]]	+	[[Category: Vincke, C]]
	[[Category: Antibody]]		[[Category: Antibody]]
	[[Category: Humanization]]		[[Category: Humanization]]

OCA: Protected "3eak" [edit=sysop:move=sysop]

OCA — Wed, 28 May 2014 10:22:00 GMT

Protected "3eak" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:22, 28 May 2014

OCA at 10:21, 28 May 2014

OCA — Wed, 28 May 2014 10:21:55 GMT

←Older revision		Revision as of 10:21, 28 May 2014
Line 1:		Line 1:
-	~~{{Seed}}~~	+	==NbBCII10 humanized (FGLA mutant)==
-	[[~~Image~~:3eak.~~png\|left~~\|200px]]	+	<StructureSection load='3eak' size='340' side='right' caption='[[3eak]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3eak]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EAK FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eak RCSB], [http://www.ebi.ac.uk/pdbsum/3eak PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/3eak_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nanobodies, single-domain antigen-binding fragments of camelid-specific heavy-chain only antibodies offer special advantages in therapy over classic antibody fragments because of their smaller size, robustness, and preference to target unique epitopes. A Nanobody differs from a human heavy chain variable domain in about ten amino acids spread all over its surface, four hallmark Nanobody-specific amino acids in the framework-2 region (positions 42, 49, 50, and 52), and a longer third antigen-binding loop (H3) folding over this area. For therapeutic applications the camelid-specific amino acid sequences in the framework have to be mutated to their human heavy chain variable domain equivalent, i.e. humanized. We performed this humanization exercise with Nanobodies of the subfamily that represents close to 80% of all dromedary-derived Nanobodies and investigated the effects on antigen affinity, solubility, expression yield, and stability. It is demonstrated that the humanization of Nanobody-specific residues outside framework-2 are neutral to the Nanobody properties. Surprisingly, the Glu-49 --> Gly and Arg-50 --> Leu humanization of hallmark amino acids generates a single domain that is more stable though probably less soluble. The other framework-2 substitutions, Phe-42 --> Val and Gly/Ala-52 --> Trp, are detrimental for antigen affinity, due to a repositioning of the H3 loop as shown by their crystal structures. These insights were used to identify a soluble, stable, well expressed universal humanized Nanobody scaffold that allows grafts of antigen-binding loops from other Nanobodies with transfer of the antigen specificity and affinity.

-	<!--	+	General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold.,Vincke C, Loris R, Saerens D, Martinez-Rodriguez S, Muyldermans S, Conrath K J Biol Chem. 2009 Jan 30;284(5):3273-84. Epub 2008 Nov 14. PMID:19010777<ref>PMID:19010777</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3eak"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3eak\| PDB=3eak \| SCENE= }}~~	+

-	~~===NbBCII10 humanized (FGLA mutant)===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_19010777}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 19010777 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_19010777}}~~	+
-		+
-	==~~About this Structure~~==	+
-	~~3EAK is a 2 chains structure of sequences from [http:~~/~~/en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA].~~	+
-		+
-	~~==Reference==~~	+
-	General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold., Vincke C, Loris R, Saerens D, Martinez-Rodriguez S, Muyldermans S, Conrath K, J Biol Chem. 2008 Nov 14. PMID:[http:/~~/www.ncbi.nlm.nih.gov/pubmed/19010777 19010777]~~	+
	[[Category: Camelus dromedarius]]		[[Category: Camelus dromedarius]]
	[[Category: Conrath, K.]]		[[Category: Conrath, K.]]
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	[[Category: Nanobody]]		[[Category: Nanobody]]
	[[Category: Vhh domain]]		[[Category: Vhh domain]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 10 15:28:40 2008''

OCA at 13:28, 10 December 2008

OCA — Wed, 10 Dec 2008 13:28:41 GMT

←Older revision		Revision as of 13:28, 10 December 2008
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	{{Seed}}		{{Seed}}
-	[[Image:3eak.~~jpg~~\|left\|200px]]	+	[[Image:3eak.png\|left\|200px]]

	<!--		<!--
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	General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold., Vincke C, Loris R, Saerens D, Martinez-Rodriguez S, Muyldermans S, Conrath K, J Biol Chem. 2008 Nov 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19010777 19010777]		General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold., Vincke C, Loris R, Saerens D, Martinez-Rodriguez S, Muyldermans S, Conrath K, J Biol Chem. 2008 Nov 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19010777 19010777]
	[[Category: Camelus dromedarius]]		[[Category: Camelus dromedarius]]
-	[[Category: ~~Pdbx_ordinal=~~, ~~<PDBx~~:~~audit_author~~.]]	+	[[Category: Conrath, K.]]
		+	[[Category: Loris, R.]]
		+	[[Category: Martinez-Rodriguez, S.]]
		+	[[Category: Muyldermans, S.]]
		+	[[Category: Saerens, D.]]
		+	[[Category: Vincke, C.]]
	[[Category: Antibody]]		[[Category: Antibody]]
	[[Category: Humanization]]		[[Category: Humanization]]
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	[[Category: Vhh domain]]		[[Category: Vhh domain]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri~~ Dec ~~5 09~~:39:09 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 10 15:28:40 2008''

OCA at 07:39, 5 December 2008

OCA — Fri, 05 Dec 2008 07:39:11 GMT

←Older revision		Revision as of 07:39, 5 December 2008
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3eak.jpg\|left\|200px]]

-	The ~~entry 3eak~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3eak", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3eak\| PDB=3eak \| SCENE= }}

-	~~Authors: Vincke, C., Loris, R., Saerens, D., Martinez-Rodriguez, S., Muyldermans, S., Conrath, K.~~	+	===NbBCII10 humanized (FGLA mutant)===

-	Description: NbBCII10 humanized (FGLA mutant)

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Sep 10 12~~:35:06 2008''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19010777}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19010777 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19010777}}
		+
		+	==About this Structure==
		+	3EAK is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EAK OCA].
		+
		+	==Reference==
		+	General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold., Vincke C, Loris R, Saerens D, Martinez-Rodriguez S, Muyldermans S, Conrath K, J Biol Chem. 2008 Nov 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19010777 19010777]
		+	[[Category: Camelus dromedarius]]
		+	[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
		+	[[Category: Antibody]]
		+	[[Category: Humanization]]
		+	[[Category: Immune system]]
		+	[[Category: Nanobody]]
		+	[[Category: Vhh domain]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Dec 5 09:39:09 2008''

OCA: New page: '''Unreleased structure''' The entry 3eak is ON HOLD until Paper Publication Authors: Vincke, C., Loris, R., Saerens, D., Martinez-Rodriguez, S., Muyldermans, S., Conrath, K. Descripti...

OCA — Wed, 10 Sep 2008 09:35:07 GMT

New page: '''Unreleased structure''' The entry 3eak is ON HOLD until Paper Publication Authors: Vincke, C., Loris, R., Saerens, D., Martinez-Rodriguez, S., Muyldermans, S., Conrath, K. Descripti...

New page

'''Unreleased structure'''

The entry 3eak is ON HOLD until Paper Publication

Authors: Vincke, C., Loris, R., Saerens, D., Martinez-Rodriguez, S., Muyldermans, S., Conrath, K.

Description: NbBCII10 humanized (FGLA mutant)

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 10 12:35:06 2008''