3efx - Revision history

OCA at 08:07, 10 April 2024

OCA — Wed, 10 Apr 2024 08:07:32 GMT

←Older revision		Revision as of 08:07, 10 April 2024
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3efx ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3efx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	A hybrid between the B subunits of cholera toxin and Escherichia coli heat-labile enterotoxin has been described, which exhibits a novel binding specificity to blood group A and B type 2 determinants. In the present investigation, we have determined the crystal structure of this protein hybrid, termed LCTBK, in complex with the blood group A pentasaccharide GalNAcalpha3(Fucalpha2)Galbeta4(Fucalpha3)GlcNAcbeta, confirming not only the novel binding specificity but also a distinct new oligosaccharide binding site. Binding studies revealed that the new specificity can be ascribed to a single mutation (S4N) introduced into the sequence of Escherichia coli heat-labile enterotoxin. At a resolution of 1.9 A, the new binding site is resolved in excellent detail. Main features include a complex network of water molecules, which is well preserved by the parent toxins, and an unexpectedly modest contribution to binding by the critical residue Asn4, which interacts with the ligand only via a single water molecule.
-
-	Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin: 1.9 A crystal structure reveals the details.,Holmner A, Lebens M, Teneberg S, Angstrom J, Okvist M, Krengel U Structure. 2004 Sep;12(9):1655-67. PMID:15341730<ref>PMID:15341730</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3efx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Cholera toxin 3D structures\|Cholera toxin 3D structures]]		*[[Cholera toxin 3D structures\|Cholera toxin 3D structures]]
	*[[User:David Solfiell/sandbox 1\|User:David Solfiell/sandbox 1]]		*[[User:David Solfiell/sandbox 1\|User:David Solfiell/sandbox 1]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:21, 1 November 2023

OCA — Wed, 01 Nov 2023 15:21:21 GMT

←Older revision		Revision as of 15:21, 1 November 2023
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	<StructureSection load='3efx' size='340' side='right'caption='[[3efx]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='3efx' size='340' side='right'caption='[[3efx]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus coli" migula 1895~~]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2nzg 2nzg] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EFX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2nzg 2nzg] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EFX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~"><~~scene name~~='~~pdbligand~~=~~BGC~~:~~BETA-D-GLUCOSE'~~>~~BGC~~</~~scene~~>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.94Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [https://pdbe.org/3efx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [https://www.ebi.ac.uk/pdbsum/3efx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3efx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [https://pdbe.org/3efx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [https://www.ebi.ac.uk/pdbsum/3efx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3efx ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ELBH_ECOLX ELBH_ECOLX] The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.[https://www.uniprot.org/uniprot/CHTB_VIBCH CHTB_VIBCH] The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus coli migula 1895]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Angstrom, J]]	+	[[Category: Vibrio cholerae]]
-	[[Category: Holmner, A]]	+	[[Category: Angstrom J]]
-	[[Category: Krengel, U]]	+	[[Category: Holmner A]]
-	[[Category: Lebens, M]]	+	[[Category: Krengel U]]
-	[[Category: Okvist, M]]	+	[[Category: Lebens M]]
-	[[Category: Teneberg, S]]	+	[[Category: Okvist M]]
-	~~[[Category: Blood group antigen]]~~	+	[[Category: Teneberg S]]
-	~~[[Category: Cholera toxin]]~~	+
-	~~[[Category: Heat-labile enterotoxin]]~~	+
-	~~[[Category: Protein-carbohydrate complex]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 11:48, 16 February 2022

OCA — Wed, 16 Feb 2022 11:48:48 GMT

←Older revision		Revision as of 11:48, 16 February 2022
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	<StructureSection load='3efx' size='340' side='right'caption='[[3efx]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='3efx' size='340' side='right'caption='[[3efx]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2nzg 2nzg] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3EFX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2nzg 2nzg] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EFX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~A2G~~:N-~~ACETYL~~-~~2-DEOXY-2-AMINO-GALACTOSE~~'>~~A2G~~</scene>, <scene name='pdbligand=~~BGC~~:~~BETA~~-D-~~GLUCOSE~~'>~~BGC~~</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [~~http~~://pdbe.org/3efx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3efx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3efx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [https://pdbe.org/3efx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [https://www.ebi.ac.uk/pdbsum/3efx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3efx ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 15:50, 29 January 2020

OCA — Wed, 29 Jan 2020 15:50:12 GMT

←Older revision		Revision as of 15:50, 29 January 2020
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	==Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details==		==Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details==
-	<StructureSection load='3efx' size='340' side='right' caption='[[3efx]], [[Resolution\|resolution]] 1.94Å' scene=''>	+	<StructureSection load='3efx' size='340' side='right'caption='[[3efx]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2nzg 2nzg] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EFX FirstGlance]. <br>		<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2nzg 2nzg] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EFX FirstGlance]. <br>
Line 28:		Line 28:

	==See Also==		==See Also==
-	*[[Cholera toxin\|Cholera toxin]]	+	*[[Cholera toxin 3D structures\|Cholera toxin 3D structures]]
	*[[User:David Solfiell/sandbox 1\|User:David Solfiell/sandbox 1]]		*[[User:David Solfiell/sandbox 1\|User:David Solfiell/sandbox 1]]
	== References ==		== References ==
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Angstrom, J]]		[[Category: Angstrom, J]]
	[[Category: Holmner, A]]		[[Category: Holmner, A]]

OCA at 21:35, 2 December 2018

OCA — Sun, 02 Dec 2018 21:35:34 GMT

←Older revision		Revision as of 21:35, 2 December 2018
Line 1:		Line 1:
		+
	==Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details==		==Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details==
	<StructureSection load='3efx' size='340' side='right' caption='[[3efx]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='3efx' size='340' side='right' caption='[[3efx]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Hybrid Hybrid~~]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2nzg 2nzg] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EFX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2nzg 2nzg] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EFX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~BGC~~:~~BETA~~-D-~~GLUCOSE~~'>~~BGC~~</scene>, <scene name='pdbligand=~~A2G~~:N-~~ACETYL~~-~~2-DEOXY-2-AMINO-GALACTOSE~~'>~~A2G~~</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [http://pdbe.org/3efx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [http://www.ebi.ac.uk/pdbsum/3efx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [http://pdbe.org/3efx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [http://www.ebi.ac.uk/pdbsum/3efx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3efx ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ef/3efx_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ef/3efx_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Hybrid~~]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Angstrom, J]]		[[Category: Angstrom, J]]
	[[Category: Holmner, A]]		[[Category: Holmner, A]]

OCA at 22:37, 7 February 2016

OCA — Sun, 07 Feb 2016 22:37:31 GMT

←Older revision		Revision as of 22:37, 7 February 2016
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Hybrid Hybrid]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2nzg 2nzg] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EFX FirstGlance]. <br>		<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Hybrid Hybrid]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2nzg 2nzg] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EFX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [http://www.ebi.ac.uk/pdbsum/3efx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [http://pdbe.org/3efx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [http://www.ebi.ac.uk/pdbsum/3efx PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3efx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3efx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 06:29, 2 September 2015

OCA — Wed, 02 Sep 2015 06:29:40 GMT

←Older revision		Revision as of 06:29, 2 September 2015
Line 2:		Line 2:
	<StructureSection load='3efx' size='340' side='right' caption='[[3efx]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='3efx' size='340' side='right' caption='[[3efx]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Hybrid Hybrid]. This structure supersedes the now removed PDB entries and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EFX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Hybrid Hybrid]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2nzg 2nzg] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EFX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [http://www.ebi.ac.uk/pdbsum/3efx PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [http://www.ebi.ac.uk/pdbsum/3efx PDBsum]</span></td></tr>

OCA at 10:12, 20 January 2015

OCA — Tue, 20 Jan 2015 10:12:34 GMT

←Older revision		Revision as of 10:12, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Hybrid Hybrid]. This structure supersedes the now removed PDB entries and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EFX FirstGlance]. <br>		<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Hybrid Hybrid]. This structure supersedes the now removed PDB entries and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EFX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [http://www.ebi.ac.uk/pdbsum/3efx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [http://www.ebi.ac.uk/pdbsum/3efx PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Hybrid]]		[[Category: Hybrid]]
-	[[Category: Angstrom, J.]]	+	[[Category: Angstrom, J]]
-	[[Category: Holmner, A.]]	+	[[Category: Holmner, A]]
-	[[Category: Krengel, U.]]	+	[[Category: Krengel, U]]
-	[[Category: Lebens, M.]]	+	[[Category: Lebens, M]]
-	[[Category: Okvist, M.]]	+	[[Category: Okvist, M]]
-	[[Category: Teneberg, S.]]	+	[[Category: Teneberg, S]]
	[[Category: Blood group antigen]]		[[Category: Blood group antigen]]
	[[Category: Cholera toxin]]		[[Category: Cholera toxin]]

OCA at 12:17, 29 September 2014

OCA — Mon, 29 Sep 2014 12:17:51 GMT

←Older revision		Revision as of 12:17, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:3efx.~~png\|left~~\|200px]]	+	==Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details==
		+	<StructureSection load='3efx' size='340' side='right' caption='[[3efx]], [[Resolution\|resolution]] 1.94Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3efx]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Hybrid Hybrid]. This structure supersedes the now removed PDB entries and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EFX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3efx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3efx RCSB], [http://www.ebi.ac.uk/pdbsum/3efx PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ef/3efx_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A hybrid between the B subunits of cholera toxin and Escherichia coli heat-labile enterotoxin has been described, which exhibits a novel binding specificity to blood group A and B type 2 determinants. In the present investigation, we have determined the crystal structure of this protein hybrid, termed LCTBK, in complex with the blood group A pentasaccharide GalNAcalpha3(Fucalpha2)Galbeta4(Fucalpha3)GlcNAcbeta, confirming not only the novel binding specificity but also a distinct new oligosaccharide binding site. Binding studies revealed that the new specificity can be ascribed to a single mutation (S4N) introduced into the sequence of Escherichia coli heat-labile enterotoxin. At a resolution of 1.9 A, the new binding site is resolved in excellent detail. Main features include a complex network of water molecules, which is well preserved by the parent toxins, and an unexpectedly modest contribution to binding by the critical residue Asn4, which interacts with the ligand only via a single water molecule.

-	~~{{STRUCTURE_3efx\| PDB=3efx \| SCENE= }}~~	+	Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin: 1.9 A crystal structure reveals the details.,Holmner A, Lebens M, Teneberg S, Angstrom J, Okvist M, Krengel U Structure. 2004 Sep;12(9):1655-67. PMID:15341730<ref>PMID:15341730</ref>

-	~~===Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin~~, ~~1.9A crystal structure reveals the details===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15341730}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3efx]] is~~ a ~~10 chain structure with sequence from [http://en.wikipedia.org/wiki/Hybrid Hybrid]. This structure supersedes~~ the ~~now removed PDB entries and [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA].~~	+

	==See Also==		==See Also==
	*[[Cholera toxin\|Cholera toxin]]		*[[Cholera toxin\|Cholera toxin]]
	*[[User:David Solfiell/sandbox 1\|User:David Solfiell/sandbox 1]]		*[[User:David Solfiell/sandbox 1\|User:David Solfiell/sandbox 1]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015341730<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Hybrid]]		[[Category: Hybrid]]
	[[Category: Angstrom, J.]]		[[Category: Angstrom, J.]]

OCA at 08:03, 20 October 2012

OCA — Sat, 20 Oct 2012 08:03:04 GMT

←Older revision		Revision as of 08:03, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3efx]] is a 10 chain structure ~~of [[Cholera toxin]] and [[User:David Solfiell/sandbox 1]]~~ with sequence from [http://en.wikipedia.org/wiki/~~Vibrio_cholerae,_escherichia_coli Vibrio cholerae, escherichia coli~~]. This structure supersedes the now removed PDB entries and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA].	+	[[3efx]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Hybrid Hybrid]. This structure supersedes the now removed PDB entries and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFX OCA].

	==See Also==		==See Also==
Line 16:		Line 16:
	==Reference==		==Reference==
	<ref group="xtra">PMID:015341730</ref><references group="xtra"/>		<ref group="xtra">PMID:015341730</ref><references group="xtra"/>
-	[[Category: ~~Vibrio cholerae, escherichia coli~~]]	+	[[Category: Hybrid]]
	[[Category: Angstrom, J.]]		[[Category: Angstrom, J.]]
	[[Category: Holmner, A.]]		[[Category: Holmner, A.]]