3egn - Revision history

OCA at 15:21, 1 November 2023

OCA — Wed, 01 Nov 2023 15:21:26 GMT

←Older revision		Revision as of 15:21, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3egn' size='340' side='right'caption='[[3egn]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3egn' size='340' side='right'caption='[[3egn]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3egn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EGN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3egn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EGN FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~U11/U12 snRNP 65K (~~[~~https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=~~5&~~id=9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3egn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egn OCA], [https://pdbe.org/3egn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3egn RCSB], [https://www.ebi.ac.uk/pdbsum/3egn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3egn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3egn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egn OCA], [https://pdbe.org/3egn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3egn RCSB], [https://www.ebi.ac.uk/pdbsum/3egn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3egn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/RNPC3_HUMAN RNPC3_HUMAN] Isolated growth hormone deficiency type IA. The disease is caused by variants affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~RBM40_HUMAN RBM40_HUMAN]~~] Participates in pre-mRNA U12-dependent splicing, performed by the minor spliceosome which removes U12-type introns. U12-type introns comprises less than 1% of all non-coding sequences. Binds to the 3'-stem-loop of m(7)G-capped U12 snRNA.<ref>PMID:16096647</ref> <ref>PMID:19447915</ref>	+	[https://www.uniprot.org/uniprot/RNPC3_HUMAN RNPC3_HUMAN] Participates in pre-mRNA U12-dependent splicing, performed by the minor spliceosome which removes U12-type introns. U12-type introns comprises less than 1% of all non-coding sequences. Binds to the 3'-stem-loop of m(7)G-capped U12 snRNA.<ref>PMID:16096647</ref> <ref>PMID:19447915</ref> <ref>PMID:24480542</ref> <ref>PMID:29255062</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Netter, C]]	+	[[Category: Netter C]]
-	[[Category: Wahl~~, M C]]~~	+	[[Category: Wahl MC]]
-	~~[[Category: Minor spliceosomal protein]]~~	+
-	~~[[Category: Mrna processing]]~~	+
-	~~[[Category: Mrna splicing]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Rna-binding]]~~	+
-	~~[[Category: Rnp motif]]~~	+
-	~~[[Category: Spliceosome]]~~	+
-	~~[[Category: Splicing]]~~	+
-	~~[[Category: U11/u12 di-snrnp]]~~	+
-	~~[[Category: U11/u12-65k protein]]~~	+
-	~~[[Category: U1a protein]]~~	+
-	~~[[Category: U2b protein~~]]	+

OCA at 11:49, 16 February 2022

OCA — Wed, 16 Feb 2022 11:49:20 GMT

←Older revision		Revision as of 11:49, 16 February 2022
Line 1:		Line 1:

	==C-terminal RNA Recognition Motif of the U11/U12 65K Protein==		==C-terminal RNA Recognition Motif of the U11/U12 65K Protein==
-	<StructureSection load='3egn' size='340' side='right' caption='[[3egn]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3egn' size='340' side='right'caption='[[3egn]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3egn]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3EGN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3egn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EGN FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U11/U12 snRNP 65K ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U11/U12 snRNP 65K ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3egn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egn OCA], [~~http~~://pdbe.org/3egn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3egn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3egn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3egn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3egn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egn OCA], [https://pdbe.org/3egn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3egn RCSB], [https://www.ebi.ac.uk/pdbsum/3egn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3egn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RBM40_HUMAN RBM40_HUMAN]] Participates in pre-mRNA U12-dependent splicing, performed by the minor spliceosome which removes U12-type introns. U12-type introns comprises less than 1% of all non-coding sequences. Binds to the 3'-stem-loop of m(7)G-capped U12 snRNA.<ref>PMID:16096647</ref> <ref>PMID:19447915</ref>	+	[[https://www.uniprot.org/uniprot/RBM40_HUMAN RBM40_HUMAN]] Participates in pre-mRNA U12-dependent splicing, performed by the minor spliceosome which removes U12-type introns. U12-type introns comprises less than 1% of all non-coding sequences. Binds to the 3'-stem-loop of m(7)G-capped U12 snRNA.<ref>PMID:16096647</ref> <ref>PMID:19447915</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Netter, C]]		[[Category: Netter, C]]
	[[Category: Wahl, M C]]		[[Category: Wahl, M C]]

OCA at 21:52, 2 December 2018

OCA — Sun, 02 Dec 2018 21:52:28 GMT

←Older revision		Revision as of 21:52, 2 December 2018
Line 1:		Line 1:
		+
	==C-terminal RNA Recognition Motif of the U11/U12 65K Protein==		==C-terminal RNA Recognition Motif of the U11/U12 65K Protein==
	<StructureSection load='3egn' size='340' side='right' caption='[[3egn]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3egn' size='340' side='right' caption='[[3egn]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3egn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EGN FirstGlance]. <br>		<table><tr><td colspan='2'>[[3egn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EGN FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U11/U12 snRNP 65K ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U11/U12 snRNP 65K ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3egn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egn OCA], [http://pdbe.org/3egn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3egn RCSB], [http://www.ebi.ac.uk/pdbsum/3egn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3egn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egn OCA], [http://pdbe.org/3egn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3egn RCSB], [http://www.ebi.ac.uk/pdbsum/3egn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3egn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/3egn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/3egn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 02:51, 8 February 2016

OCA — Mon, 08 Feb 2016 02:51:46 GMT

←Older revision		Revision as of 02:51, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='3egn' size='340' side='right' caption='[[3egn]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3egn' size='340' side='right' caption='[[3egn]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3egn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EGN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3egn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EGN FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U11/U12 snRNP 65K ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U11/U12 snRNP 65K ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3egn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3egn RCSB], [http://www.ebi.ac.uk/pdbsum/3egn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3egn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egn OCA], [http://pdbe.org/3egn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3egn RCSB], [http://www.ebi.ac.uk/pdbsum/3egn PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3egn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3egn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Netter, C]]		[[Category: Netter, C]]
	[[Category: Wahl, M C]]		[[Category: Wahl, M C]]

OCA at 20:02, 24 December 2014

OCA — Wed, 24 Dec 2014 20:02:44 GMT

←Older revision		Revision as of 20:02, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3egn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3egn RCSB], [http://www.ebi.ac.uk/pdbsum/3egn PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3egn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3egn RCSB], [http://www.ebi.ac.uk/pdbsum/3egn PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RBM40_HUMAN RBM40_HUMAN]] Participates in pre-mRNA U12-dependent splicing, performed by the minor spliceosome which removes U12-type introns. U12-type introns comprises less than 1% of all non-coding sequences. Binds to the 3'-stem-loop of m(7)G-capped U12 snRNA.<ref>PMID:16096647</ref> <ref>PMID:19447915</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 13:16, 19 November 2014

OCA — Wed, 19 Nov 2014 13:16:16 GMT

←Older revision		Revision as of 13:16, 19 November 2014
Line 1:		Line 1:
-	[[~~Image~~:3egn.~~png\|left~~\|200px]]	+	==C-terminal RNA Recognition Motif of the U11/U12 65K Protein==
		+	<StructureSection load='3egn' size='340' side='right' caption='[[3egn]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3egn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EGN FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U11/U12 snRNP 65K ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3egn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3egn RCSB], [http://www.ebi.ac.uk/pdbsum/3egn PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/3egn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RNA recognition motifs (RRMs) constitute versatile macromolecular interaction platforms. They are found in many components of spliceosomes, in which they mediate RNA and protein interactions by diverse molecular strategies. The human U11/U12-65K protein of the minor spliceosome employs a C-terminal RRM to bind hairpin III of the U12 small nuclear RNA (snRNA). This interaction comprises one side of a molecular bridge between the U11 and U12 small nuclear ribonucleoprotein particles (snRNPs) and is reminiscent of the binding of the N-terminal RRMs in the major spliceosomal U1A and U2B'' proteins to hairpins in their cognate snRNAs. Here we show by mutagenesis and electrophoretic mobility shift assays that the beta-sheet surface and a neighboring loop of 65K C-terminal RRM are involved in RNA binding, as previously seen in canonical RRMs like the N-terminal RRMs of the U1A and U2B'' proteins. However, unlike U1A and U2B'', some 30 residues N-terminal of the 65K C-terminal RRM core are additionally required for stable U12 snRNA binding. The crystal structure of the expanded 65K C-terminal RRM revealed that the N-terminal tail adopts an alpha-helical conformation and wraps around the protein toward the face opposite the RNA-binding platform. Point mutations in this part of the protein had only minor effects on RNA affinity. Removal of the N-terminal extension significantly decreased the thermal stability of the 65K C-terminal RRM. These results demonstrate that the 65K C-terminal RRM is augmented by an N-terminal element that confers stability to the domain, and thereby facilitates stable RNA binding.

-	~~{{STRUCTURE_3egn\| PDB=3egn \| SCENE= }}~~	+	Functional stabilization of an RNA recognition motif by a noncanonical N-terminal expansion.,Netter C, Weber G, Benecke H, Wahl MC RNA. 2009 Jul;15(7):1305-13. Epub 2009 May 15. PMID:19447915<ref>PMID:19447915</ref>

-	~~===C-terminal RNA Recognition Motif~~ of the ~~U11/U12 65K Protein===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19447915}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3egn]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019447915<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Netter, C.]]	+	[[Category: Netter, C]]
-	[[Category: Wahl, M C.]]	+	[[Category: Wahl, M C]]
	[[Category: Minor spliceosomal protein]]		[[Category: Minor spliceosomal protein]]
	[[Category: Mrna processing]]		[[Category: Mrna processing]]

OCA: Protected "3egn" [edit=sysop:move=sysop]

OCA — Wed, 09 Jan 2013 08:39:58 GMT

Protected "3egn" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:39, 9 January 2013

OCA at 08:39, 9 January 2013

OCA — Wed, 09 Jan 2013 08:39:57 GMT

←Older revision		Revision as of 08:39, 9 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3egn.png\|left\|200px]]		[[Image:3egn.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3egn", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3egn\| PDB=3egn \| SCENE= }}		{{STRUCTURE_3egn\| PDB=3egn \| SCENE= }}

	===C-terminal RNA Recognition Motif of the U11/U12 65K Protein===		===C-terminal RNA Recognition Motif of the U11/U12 65K Protein===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19447915}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19447915 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19447915}}		{{ABSTRACT_PUBMED_19447915}}

	==About this Structure==		==About this Structure==
-	~~3EGN~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA].	+	[[3egn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19447915~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019447915</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Netter, C.]]		[[Category: Netter, C.]]
	[[Category: Wahl, M C.]]		[[Category: Wahl, M C.]]
-	[[Category: Crystal structure]]
	[[Category: Minor spliceosomal protein]]		[[Category: Minor spliceosomal protein]]
	[[Category: Mrna processing]]		[[Category: Mrna processing]]
Line 36:		Line 23:
	[[Category: Rnp motif]]		[[Category: Rnp motif]]
	[[Category: Spliceosome]]		[[Category: Spliceosome]]
		+	[[Category: Splicing]]
	[[Category: U11/u12 di-snrnp]]		[[Category: U11/u12 di-snrnp]]
	[[Category: U11/u12-65k protein]]		[[Category: U11/u12-65k protein]]
	[[Category: U1a protein]]		[[Category: U1a protein]]
	[[Category: U2b protein]]		[[Category: U2b protein]]
-	[[Category: X-ray crystallography]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 1 09:52:55 2009''

OCA at 06:52, 1 July 2009

OCA — Wed, 01 Jul 2009 06:52:57 GMT

←Older revision		Revision as of 06:52, 1 July 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3egn.~~jpg~~\|left\|200px]]	+	[[Image:3egn.png\|left\|200px]]

	<!--		<!--
Line 42:		Line 42:
	[[Category: X-ray crystallography]]		[[Category: X-ray crystallography]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jun 10 18~~:01:19 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 1 09:52:55 2009''

OCA at 15:01, 10 June 2009

OCA — Wed, 10 Jun 2009 15:01:21 GMT

←Older revision		Revision as of 15:01, 10 June 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3egn.jpg\|left\|200px]]

-	The ~~entry 3egn~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3egn", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3egn\| PDB=3egn \| SCENE= }}

-	~~Authors: Netter,~~ C~~., Weber, G., Benecke, H., Wahl, M.C.~~	+	===C-terminal RNA Recognition Motif of the U11/U12 65K Protein===

-	Description: C-terminal RNA Recognition Motif of the U11/U12 65K Protein

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 24~~ 10:15:~~26 2008~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19447915}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19447915 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19447915}}
		+
		+	==About this Structure==
		+	3EGN is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGN OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19447915</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Netter, C.]]
		+	[[Category: Wahl, M C.]]
		+	[[Category: Crystal structure]]
		+	[[Category: Minor spliceosomal protein]]
		+	[[Category: Mrna processing]]
		+	[[Category: Mrna splicing]]
		+	[[Category: Nucleus]]
		+	[[Category: Phosphoprotein]]
		+	[[Category: Rna-binding]]
		+	[[Category: Rnp motif]]
		+	[[Category: Spliceosome]]
		+	[[Category: U11/u12 di-snrnp]]
		+	[[Category: U11/u12-65k protein]]
		+	[[Category: U1a protein]]
		+	[[Category: U2b protein]]
		+	[[Category: X-ray crystallography]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 10 18:01:19 2009''