3emr - Revision history

OCA at 09:10, 23 February 2022

OCA — Wed, 23 Feb 2022 09:10:13 GMT

←Older revision		Revision as of 09:10, 23 February 2022
Line 1:		Line 1:

	==Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens==		==Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens==
-	<StructureSection load='3emr' size='340' side='right' caption='[[3emr]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='3emr' size='340' side='right'caption='[[3emr]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3emr]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_700290 Atcc 700290]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EMR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3EMR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3emr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_700290 Atcc 700290]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EMR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EMR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ectD ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=61016 ATCC 700290])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ectD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=61016 ATCC 700290])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3emr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3emr OCA], [~~http~~://pdbe.org/3emr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3emr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3emr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3emr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3emr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3emr OCA], [https://pdbe.org/3emr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3emr RCSB], [https://www.ebi.ac.uk/pdbsum/3emr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3emr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ECTD_VIRSA ECTD_VIRSA]] Involved in the biosynthesis of ectoine ((S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid) which is a highly soluble organic osmolyte, called compatible solute, use to avoid excessive water efflux, plasmolysis, molecular crowding of the cytoplasm, and cessation of growth in high salinity environments. Catalyzes the 2-oxoglutarate-dependent selective hydroxylation of L-ectoine to yield (4S,5S)-5-hydroxyectoine.<ref>PMID:17636255</ref>	+	[[https://www.uniprot.org/uniprot/ECTD_VIRSA ECTD_VIRSA]] Involved in the biosynthesis of ectoine ((S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid) which is a highly soluble organic osmolyte, called compatible solute, use to avoid excessive water efflux, plasmolysis, molecular crowding of the cytoplasm, and cessation of growth in high salinity environments. Catalyzes the 2-oxoglutarate-dependent selective hydroxylation of L-ectoine to yield (4S,5S)-5-hydroxyectoine.<ref>PMID:17636255</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/em/3emr_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/em/3emr_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 3emr" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3emr" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Hydroxylases 3D structures\|Hydroxylases 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 700290]]		[[Category: Atcc 700290]]
		+	[[Category: Large Structures]]
	[[Category: Heine, A]]		[[Category: Heine, A]]
	[[Category: Reuter, K]]		[[Category: Reuter, K]]
	[[Category: Double stranded beta helix]]		[[Category: Double stranded beta helix]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 09:15, 25 October 2017

OCA — Wed, 25 Oct 2017 09:15:12 GMT

←Older revision		Revision as of 09:15, 25 October 2017
Line 1:		Line 1:
		+
	==Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens==		==Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens==
	<StructureSection load='3emr' size='340' side='right' caption='[[3emr]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3emr' size='340' side='right' caption='[[3emr]], [[Resolution\|resolution]] 1.85Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ectD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=61016 ATCC 700290])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ectD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=61016 ATCC 700290])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3emr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3emr OCA], [http://pdbe.org/3emr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3emr RCSB], [http://www.ebi.ac.uk/pdbsum/3emr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3emr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3emr OCA], [http://pdbe.org/3emr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3emr RCSB], [http://www.ebi.ac.uk/pdbsum/3emr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3emr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 18:40, 9 February 2016

OCA — Tue, 09 Feb 2016 18:40:34 GMT

←Older revision		Revision as of 18:40, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3emr' size='340' side='right' caption='[[3emr]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3emr' size='340' side='right' caption='[[3emr]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3emr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Virgibacillus_salexigens Virgibacillus salexigens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EMR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EMR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3emr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_700290 Atcc 700290]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EMR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EMR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ectD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=61016 ~~Virgibacillus salexigens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ectD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=61016 ATCC 700290])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3emr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3emr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3emr RCSB], [http://www.ebi.ac.uk/pdbsum/3emr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3emr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3emr OCA], [http://pdbe.org/3emr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3emr RCSB], [http://www.ebi.ac.uk/pdbsum/3emr PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q2TDY4_9BACI Q2TDY4_9BACI~~]] Involved in the biosynthesis of ectoine ((S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid) which is a highly soluble organic osmolyte, called compatible solute, use to avoid excessive water efflux, plasmolysis, molecular crowding of the cytoplasm, and cessation of growth in high salinity environments. Catalyzes the 2-oxoglutarate-dependent selective hydroxylation of L-ectoine to yield (4S,5S)-5-hydroxyectoine.<ref>PMID:17636255</ref>	+	[[http://www.uniprot.org/uniprot/ECTD_VIRSA ECTD_VIRSA]] Involved in the biosynthesis of ectoine ((S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid) which is a highly soluble organic osmolyte, called compatible solute, use to avoid excessive water efflux, plasmolysis, molecular crowding of the cytoplasm, and cessation of growth in high salinity environments. Catalyzes the 2-oxoglutarate-dependent selective hydroxylation of L-ectoine to yield (4S,5S)-5-hydroxyectoine.<ref>PMID:17636255</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3emr ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3emr" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Virgibacillus salexigens~~]]	+	[[Category: Atcc 700290]]
	[[Category: Heine, A]]		[[Category: Heine, A]]
	[[Category: Reuter, K]]		[[Category: Reuter, K]]
	[[Category: Double stranded beta helix]]		[[Category: Double stranded beta helix]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 08:44, 25 December 2014

OCA — Thu, 25 Dec 2014 08:44:40 GMT

←Older revision		Revision as of 08:44, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3emr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3emr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3emr RCSB], [http://www.ebi.ac.uk/pdbsum/3emr PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3emr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3emr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3emr RCSB], [http://www.ebi.ac.uk/pdbsum/3emr PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q2TDY4_9BACI Q2TDY4_9BACI]] Involved in the biosynthesis of ectoine ((S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid) which is a highly soluble organic osmolyte, called compatible solute, use to avoid excessive water efflux, plasmolysis, molecular crowding of the cytoplasm, and cessation of growth in high salinity environments. Catalyzes the 2-oxoglutarate-dependent selective hydroxylation of L-ectoine to yield (4S,5S)-5-hydroxyectoine.<ref>PMID:17636255</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 13:31, 19 November 2014

OCA — Wed, 19 Nov 2014 13:31:00 GMT

←Older revision		Revision as of 13:31, 19 November 2014
Line 1:		Line 1:
-	[[~~Image:~~3emr.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens==
		+	<StructureSection load='3emr' size='340' side='right' caption='[[3emr]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3emr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Virgibacillus_salexigens Virgibacillus salexigens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EMR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EMR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ectD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=61016 Virgibacillus salexigens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3emr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3emr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3emr RCSB], [http://www.ebi.ac.uk/pdbsum/3emr PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/em/3emr_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	As a response to high osmolality, many microorganisms synthesize various types of compatible solutes. These organic osmolytes aid in offsetting the detrimental effects of low water activity on cell physiology. One of these compatible solutes is ectoine. A sub-group of the ectoine producer's enzymatically convert this tetrahydropyrimidine into a hydroxylated derivative, 5-hydroxyectoine. This compound also functions as an effective osmostress protectant and compatible solute but it possesses properties that differ in several aspects from those of ectoine. The enzyme responsible for ectoine hydroxylation (EctD) is a member of the non-heme iron(II)-containing and 2-oxoglutarate-dependent dioxygenases (EC 1.14.11). These enzymes couple the decarboxylation of 2-oxoglutarate with the formation of a high-energy ferryl-oxo intermediate to catalyze the oxidation of the bound organic substrate. We report here the crystal structure of the ectoine hydroxylase EctD from the moderate halophile Virgibacillus salexigens in complex with Fe(3+) at a resolution of 1.85 A. Like other non-heme iron(II) and 2-oxoglutarate dependent dioxygenases, the core of the EctD structure consists of a double-stranded beta-helix forming the main portion of the active-site of the enzyme. The positioning of the iron ligand in the active-site of EctD is mediated by an evolutionarily conserved 2-His-1-carboxylate iron-binding motif. The side chains of the three residues forming this iron-binding site protrude into a deep cavity in the EctD structure that also harbours the 2-oxoglutarate co-substrate-binding site. Database searches revealed a widespread occurrence of EctD-type proteins in members of the Bacteria but only in a single representative of the Archaea, the marine crenarchaeon Nitrosopumilus maritimus. The EctD crystal structure reported here can serve as a template to guide further biochemical and structural studies of this biotechnologically interesting enzyme family.

-	~~{{STRUCTURE_3emr\| PDB=3emr \| SCENE= }}~~	+	Synthesis of 5-hydroxyectoine from ectoine: crystal structure of the non-heme iron(II) and 2-oxoglutarate-dependent dioxygenase EctD.,Reuter K, Pittelkow M, Bursy J, Heine A, Craan T, Bremer E PLoS One. 2010 May 14;5(5):e10647. PMID:20498719<ref>PMID:20498719</ref>

-	~~===Crystal Structure Analysis~~ of the ~~ectoine hydroxylase ECTD from Salibacillus salexigens===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20498719}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3emr]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Virgibacillus_salexigens Virgibacillus salexigens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3EMR OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:020498719<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Virgibacillus salexigens]]		[[Category: Virgibacillus salexigens]]
-	[[Category: Heine, A.]]	+	[[Category: Heine, A]]
-	[[Category: Reuter, K.]]	+	[[Category: Reuter, K]]
	[[Category: Double stranded beta helix]]		[[Category: Double stranded beta helix]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA: Protected "3emr" [edit=sysop:move=sysop]

OCA — Wed, 06 Feb 2013 13:37:19 GMT

Protected "3emr" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:37, 6 February 2013

OCA at 13:37, 6 February 2013

OCA — Wed, 06 Feb 2013 13:37:17 GMT

←Older revision		Revision as of 13:37, 6 February 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3emr.png\|left\|200px]]		[[Image:3emr.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3emr", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3emr\| PDB=3emr \| SCENE= }}		{{STRUCTURE_3emr\| PDB=3emr \| SCENE= }}

	===Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens===		===Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20498719}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20498719 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20498719}}		{{ABSTRACT_PUBMED_20498719}}

	==About this Structure==		==About this Structure==
-	~~3EMR~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Virgibacillus_salexigens Virgibacillus salexigens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EMR OCA].	+	[[3emr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Virgibacillus_salexigens Virgibacillus salexigens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EMR OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20498719~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020498719</ref><references group="xtra"/>
	[[Category: Virgibacillus salexigens]]		[[Category: Virgibacillus salexigens]]
	[[Category: Heine, A.]]		[[Category: Heine, A.]]
Line 29:		Line 17:
	[[Category: Double stranded beta helix]]		[[Category: Double stranded beta helix]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 18 11:06:03 2010''

OCA at 06:58, 18 August 2010

OCA — Wed, 18 Aug 2010 06:58:29 GMT

←Older revision		Revision as of 06:58, 18 August 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3emr.~~jpg~~\|left\|200px]]	+	[[Image:3emr.png\|left\|200px]]

	<!--		<!--
Line 12:		Line 12:
	===Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens===		===Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20498719}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20498719 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20498719}}

	==About this Structure==		==About this Structure==
-	3EMR is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Virgibacillus_salexigens Virgibacillus salexigens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EMR OCA].	+	3EMR is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Virgibacillus_salexigens Virgibacillus salexigens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EMR OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20498719</ref><references group="xtra"/>
	[[Category: Virgibacillus salexigens]]		[[Category: Virgibacillus salexigens]]
	[[Category: Heine, A.]]		[[Category: Heine, A.]]
Line 21:		Line 30:
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 30 09:26~~:06 ~~2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 18 11:06:03 2010''

OCA at 07:26, 30 September 2009

OCA — Wed, 30 Sep 2009 07:26:07 GMT

←Older revision		Revision as of 07:26, 30 September 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3emr.jpg\|left\|200px]]

-	The ~~entry 3emr~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3emr", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3emr\| PDB=3emr \| SCENE= }}

-	~~Authors: Reuter, K., Heine, A.~~	+	===Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens===

-	Description: Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Sep ~~3 15~~:16:20 2009''	+	==About this Structure==
		+	3EMR is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Virgibacillus_salexigens Virgibacillus salexigens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EMR OCA].
		+	[[Category: Virgibacillus salexigens]]
		+	[[Category: Heine, A.]]
		+	[[Category: Reuter, K.]]
		+	[[Category: Double stranded beta helix]]
		+	[[Category: Oxidoreductase]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 30 09:26:06 2009''

OCA at 12:16, 3 September 2009

OCA — Thu, 03 Sep 2009 12:16:21 GMT

←Older revision		Revision as of 12:16, 3 September 2009
Line 3:		Line 3:
	The entry 3emr is ON HOLD until Paper Publication		The entry 3emr is ON HOLD until Paper Publication

-	Authors: Reuter, K~~., Bursy, J~~., Heine, A~~., Pittelkow, M., Bremer, E~~.	+	Authors: Reuter, K., Heine, A.

	Description: Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens		Description: Crystal Structure Analysis of the ectoine hydroxylase ECTD from Salibacillus salexigens

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Oct 29 10~~:28:~~09 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 15:16:20 2009''