3era - Revision history

OCA at 06:36, 3 April 2024

OCA — Wed, 03 Apr 2024 06:36:56 GMT

←Older revision		Revision as of 06:36, 3 April 2024
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	<StructureSection load='3era' size='340' side='right'caption='[[3era]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3era' size='340' side='right'caption='[[3era]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3era]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Black-banded_sea_krait Black-banded sea krait~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ERA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3era]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ERA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [https://pdbe.org/3era PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [https://www.ebi.ac.uk/pdbsum/3era PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3era ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [https://pdbe.org/3era PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [https://www.ebi.ac.uk/pdbsum/3era PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3era ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~NXSA_PSSEM NXSA_PSSEM]~~] Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs) and with low affinity to neuronal alpha-7 nAChRs and ~~inhibit~~ acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission~~. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site~~. Blocks the extracellular increase of dopamine evoked by nicotine at 4.2 uM concentrations.<ref>PMID:9305882</ref> <ref>PMID:9840221</ref>	+	[https://www.uniprot.org/uniprot/3S1EA_LATSE 3S1EA_LATSE] Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs) (tested on Torpedo marmorata, Kd=21 uM) and with low affinity to neuronal alpha-7/CHRNA7 nAChRs (tested on chimeric alpha-7/CHRNA7, Kd=0.07 nM) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission (PubMed:7721859). Blocks the extracellular increase of dopamine evoked by nicotine at 4.2 uM concentrations (PubMed:9840221). In vivo, produces peripheral paralysis.<ref>PMID:7721859</ref> <ref>PMID:9305882</ref> <ref>PMID:9840221</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3era ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3era ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	A previous mutational analysis of erabutoxin a (Ea), a curaremimetic toxin from sea snake venom, showed that the substitutions S8G and S8T caused, respectively, 176-fold and 780-fold affinity decreases for the nicotinic acetylcholine receptor (AchR). In view of the fact that the side-chain of Ser8 is buried in the wild-type toxin, we wondered whether these affinity changes reflect a direct binding contribution of S8 to the receptor and/or conformational changes that could have occurred in Ea as a result of the introduced mutations. To approach this question, we solved X-ray structures of the two mutants S8G and S8T at high resolution (0.18 nm and 0.17 nm, with R factors of 18.0% and 17.9%, respectively). The data show that none of the mutations significantly modified the toxin structure. Even within the site where the toxin binds to the receptor the backbone conformation remained unchanged. Therefore, the low affinities of the mutants S8T and S8G cannot be explained by a large conformational change of the toxin structure. Although we cannot exclude the possibility that undetectable structural changes have occurred in the toxin mutants, our data support the view that, although buried between loop I and II, S8 is part of the functional epitope of the toxin.
-
-	High resolution x-ray analysis of two mutants of a curaremimetic snake toxin.,Gaucher JF, Menez R, Arnoux B, Pusset J, Ducruix A Eur J Biochem. 2000 Mar;267(5):1323-9. PMID:10691969<ref>PMID:10691969</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3era" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Black-banded sea krait]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Arnoux, B]]	+	[[Category: Laticauda semifasciata]]
-	[[Category: Ducruix, A]]	+	[[Category: Arnoux B]]
-	[[Category: Gaucher~~, J F~~]]	+	[[Category: Ducruix A]]
-	[[Category: Menez, A]]	+	[[Category: Gaucher JF]]
-	[[Category: Menez, R]]	+	[[Category: Menez A]]
-	~~[[Category: Neurotoxin]]~~	+	[[Category: Menez R]]
-	~~[[Category: Postsynaptic neurotoxin]]~~	+
-	~~[[Category: Snake neurotoxin]]~~	+
-	~~[[Category: Venom~~]]	+

OCA at 15:46, 3 November 2021

OCA — Wed, 03 Nov 2021 15:46:59 GMT

←Older revision		Revision as of 15:46, 3 November 2021
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	<StructureSection load='3era' size='340' side='right'caption='[[3era]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3era' size='340' side='right'caption='[[3era]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3era]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Black-banded_sea_krait Black-banded sea krait]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3ERA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3era]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Black-banded_sea_krait Black-banded sea krait]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ERA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [~~http~~://pdbe.org/3era PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3era PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3era ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [https://pdbe.org/3era PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [https://www.ebi.ac.uk/pdbsum/3era PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3era ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NXSA_PSSEM NXSA_PSSEM]] Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs) and with low affinity to neuronal alpha-7 nAChRs and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Blocks the extracellular increase of dopamine evoked by nicotine at 4.2 uM concentrations.<ref>PMID:9305882</ref> <ref>PMID:9840221</ref>	+	[[https://www.uniprot.org/uniprot/NXSA_PSSEM NXSA_PSSEM]] Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs) and with low affinity to neuronal alpha-7 nAChRs and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Blocks the extracellular increase of dopamine evoked by nicotine at 4.2 uM concentrations.<ref>PMID:9305882</ref> <ref>PMID:9840221</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:24, 9 September 2020

OCA — Wed, 09 Sep 2020 10:24:27 GMT

←Older revision		Revision as of 10:24, 9 September 2020
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	==RECOMBINANT ERABUTOXIN A (S8T MUTANT)==		==RECOMBINANT ERABUTOXIN A (S8T MUTANT)==
-	<StructureSection load='3era' size='340' side='right' caption='[[3era]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='3era' size='340' side='right'caption='[[3era]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3era]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Black-banded_sea_krait Black-banded sea krait]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ERA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3era]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Black-banded_sea_krait Black-banded sea krait]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3ERA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [http://pdbe.org/3era PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [http://www.ebi.ac.uk/pdbsum/3era PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3era ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [http://pdbe.org/3era PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [http://www.ebi.ac.uk/pdbsum/3era PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3era ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Black-banded sea krait]]		[[Category: Black-banded sea krait]]
		+	[[Category: Large Structures]]
	[[Category: Arnoux, B]]		[[Category: Arnoux, B]]
	[[Category: Ducruix, A]]		[[Category: Ducruix, A]]

OCA at 21:51, 2 December 2018

OCA — Sun, 02 Dec 2018 21:51:34 GMT

←Older revision		Revision as of 21:51, 2 December 2018
Line 1:		Line 1:
		+
	==RECOMBINANT ERABUTOXIN A (S8T MUTANT)==		==RECOMBINANT ERABUTOXIN A (S8T MUTANT)==
	<StructureSection load='3era' size='340' side='right' caption='[[3era]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3era' size='340' side='right' caption='[[3era]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3era]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Black-banded_sea_krait Black-banded sea krait]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ERA FirstGlance]. <br>		<table><tr><td colspan='2'>[[3era]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Black-banded_sea_krait Black-banded sea krait]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ERA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [http://pdbe.org/3era PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [http://www.ebi.ac.uk/pdbsum/3era PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [http://pdbe.org/3era PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [http://www.ebi.ac.uk/pdbsum/3era PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3era ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/er/3era_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/er/3era_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 02:52, 7 February 2016

OCA — Sun, 07 Feb 2016 02:52:35 GMT

←Older revision		Revision as of 02:52, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='3era' size='340' side='right' caption='[[3era]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3era' size='340' side='right' caption='[[3era]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3era]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Laticauda_semifasciata Laticauda semifasciata~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ERA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3era]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Black-banded_sea_krait Black-banded sea krait]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ERA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [http://www.ebi.ac.uk/pdbsum/3era PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [http://pdbe.org/3era PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [http://www.ebi.ac.uk/pdbsum/3era PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~NXSA_LATSE NXSA_LATSE~~]] Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs), and with low affinity to neuronal alpha-7 nAChRs. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Blocks the extracellular increase of dopamine evoked by nicotine at 4.2 uM concentrations.<ref>PMID:9305882</ref> <ref>PMID:9840221</ref>	+	[[http://www.uniprot.org/uniprot/NXSA_PSSEM NXSA_PSSEM]] Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs) and with low affinity to neuronal alpha-7 nAChRs and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Blocks the extracellular increase of dopamine evoked by nicotine at 4.2 uM concentrations.<ref>PMID:9305882</ref> <ref>PMID:9840221</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3era ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3era" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Laticauda semifasciata~~]]	+	[[Category: Black-banded sea krait]]
	[[Category: Arnoux, B]]		[[Category: Arnoux, B]]
	[[Category: Ducruix, A]]		[[Category: Ducruix, A]]

OCA at 07:15, 24 December 2014

OCA — Wed, 24 Dec 2014 07:15:30 GMT

←Older revision		Revision as of 07:15, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [http://www.ebi.ac.uk/pdbsum/3era PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [http://www.ebi.ac.uk/pdbsum/3era PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NXSA_LATSE NXSA_LATSE]] Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs), and with low affinity to neuronal alpha-7 nAChRs. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Blocks the extracellular increase of dopamine evoked by nicotine at 4.2 uM concentrations.<ref>PMID:9305882</ref> <ref>PMID:9840221</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:35, 26 November 2014

OCA — Wed, 26 Nov 2014 08:35:40 GMT

←Older revision		Revision as of 08:35, 26 November 2014
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-	[[~~Image~~:3era.~~png\|left~~\|200px]]	+	==RECOMBINANT ERABUTOXIN A (S8T MUTANT)==
		+	<StructureSection load='3era' size='340' side='right' caption='[[3era]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3era]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ERA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3era OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3era RCSB], [http://www.ebi.ac.uk/pdbsum/3era PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/er/3era_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A previous mutational analysis of erabutoxin a (Ea), a curaremimetic toxin from sea snake venom, showed that the substitutions S8G and S8T caused, respectively, 176-fold and 780-fold affinity decreases for the nicotinic acetylcholine receptor (AchR). In view of the fact that the side-chain of Ser8 is buried in the wild-type toxin, we wondered whether these affinity changes reflect a direct binding contribution of S8 to the receptor and/or conformational changes that could have occurred in Ea as a result of the introduced mutations. To approach this question, we solved X-ray structures of the two mutants S8G and S8T at high resolution (0.18 nm and 0.17 nm, with R factors of 18.0% and 17.9%, respectively). The data show that none of the mutations significantly modified the toxin structure. Even within the site where the toxin binds to the receptor the backbone conformation remained unchanged. Therefore, the low affinities of the mutants S8T and S8G cannot be explained by a large conformational change of the toxin structure. Although we cannot exclude the possibility that undetectable structural changes have occurred in the toxin mutants, our data support the view that, although buried between loop I and II, S8 is part of the functional epitope of the toxin.

-	~~{{STRUCTURE_3era\| PDB=3era \| SCENE= }}~~	+	High resolution x-ray analysis of two mutants of a curaremimetic snake toxin.,Gaucher JF, Menez R, Arnoux B, Pusset J, Ducruix A Eur J Biochem. 2000 Mar;267(5):1323-9. PMID:10691969<ref>PMID:10691969</ref>

-	~~===RECOMBINANT ERABUTOXIN A (S8T MUTANT)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10691969}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3era]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Laticauda_semifasciata Laticauda semifasciata]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:010691969<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Laticauda semifasciata]]		[[Category: Laticauda semifasciata]]
-	[[Category: Arnoux, B.]]	+	[[Category: Arnoux, B]]
-	[[Category: Ducruix, A.]]	+	[[Category: Ducruix, A]]
-	[[Category: Gaucher, J F.]]	+	[[Category: Gaucher, J F]]
-	[[Category: Menez, A.]]	+	[[Category: Menez, A]]
-	[[Category: Menez, R.]]	+	[[Category: Menez, R]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
	[[Category: Postsynaptic neurotoxin]]		[[Category: Postsynaptic neurotoxin]]
	[[Category: Snake neurotoxin]]		[[Category: Snake neurotoxin]]
	[[Category: Venom]]		[[Category: Venom]]

OCA: Protected "3era" [edit=sysop:move=sysop]

OCA — Wed, 06 Feb 2013 13:18:52 GMT

Protected "3era" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:18, 6 February 2013

OCA at 13:18, 6 February 2013

OCA — Wed, 06 Feb 2013 13:18:48 GMT

←Older revision		Revision as of 13:18, 6 February 2013
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-	{{Seed}}
	[[Image:3era.png\|left\|200px]]		[[Image:3era.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3era", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3era\| PDB=3era \| SCENE= }}		{{STRUCTURE_3era\| PDB=3era \| SCENE= }}

	===RECOMBINANT ERABUTOXIN A (S8T MUTANT)===		===RECOMBINANT ERABUTOXIN A (S8T MUTANT)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10691969}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10691969 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_10691969}}		{{ABSTRACT_PUBMED_10691969}}

	==About this Structure==		==About this Structure==
-	~~3ERA~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA].	+	[[3era]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10691969~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010691969</ref><references group="xtra"/>
	[[Category: Laticauda semifasciata]]		[[Category: Laticauda semifasciata]]
	[[Category: Arnoux, B.]]		[[Category: Arnoux, B.]]
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	[[Category: Menez, A.]]		[[Category: Menez, A.]]
	[[Category: Menez, R.]]		[[Category: Menez, R.]]
		+	[[Category: Neurotoxin]]
	[[Category: Postsynaptic neurotoxin]]		[[Category: Postsynaptic neurotoxin]]
	[[Category: Snake neurotoxin]]		[[Category: Snake neurotoxin]]
	[[Category: Venom]]		[[Category: Venom]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 10:32:17 2009''

OCA at 08:32, 16 February 2009

OCA — Mon, 16 Feb 2009 08:32:20 GMT

←Older revision		Revision as of 08:32, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3ERA is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA].	+	3ERA is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERA OCA].

	==Reference==		==Reference==
-	~~High resolution x-ray analysis of two mutants of a curaremimetic snake toxin., Gaucher JF, Menez R, Arnoux B, Pusset J, Ducruix A, Eur J Biochem. 2000 Mar;267(5):1323-9.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/10691969 10691969]~~	+	<ref group="xtra">PMID:10691969</ref><references group="xtra"/>
	[[Category: Laticauda semifasciata]]		[[Category: Laticauda semifasciata]]
-	[[Category: Single protein]]
	[[Category: Arnoux, B.]]		[[Category: Arnoux, B.]]
	[[Category: Ducruix, A.]]		[[Category: Ducruix, A.]]
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	[[Category: Venom]]		[[Category: Venom]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 12~~:44:~~56 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 10:32:17 2009''