3fan - Revision history

OCA at 00:30, 28 December 2023

OCA — Thu, 28 Dec 2023 00:30:13 GMT

←Older revision		Revision as of 00:30, 28 December 2023
Line 3:		Line 3:
	<StructureSection load='3fan' size='340' side='right'caption='[[3fan]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3fan' size='340' side='right'caption='[[3fan]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fan]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Prrsv Prrsv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FAN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fan]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_reproductive_and_respiratory_syndrome_virus Porcine reproductive and respiratory syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FAN FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[1mbm\|1mbm]]~~, [[~~3fao~~\|~~3fao~~]]~~</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Nsp4, ORF1a ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~28344 PRRSV])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>[https://en.wikipedia.org/wiki/Equine_arterivirus_serine_peptidase Equine arterivirus serine peptidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.114 3.4.21.114] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fan OCA], [https://pdbe.org/3fan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fan RCSB], [https://www.ebi.ac.uk/pdbsum/3fan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fan ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fan OCA], [https://pdbe.org/3fan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fan RCSB], [https://www.ebi.ac.uk/pdbsum/3fan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fan ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A1E8J1_PRRSV A1E8J1_PRRSV]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Equine arterivirus serine peptidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Prrsv~~]]	+	[[Category: Porcine reproductive and respiratory syndrome virus]]
-	[[Category: Gao, F]]	+	[[Category: Gao F]]
-	[[Category: Gao~~, G F~~]]	+	[[Category: Gao GF]]
-	[[Category: Liu, Y]]	+	[[Category: Liu Y]]
-	[[Category: Lu, G]]	+	[[Category: Lu G]]
-	[[Category: Peng, H]]	+	[[Category: Peng H]]
-	[[Category: Tian, X]]	+	[[Category: Tian X]]
-	~~[[Category: C-terminal alpha-beta extra domain]]~~	+
-	~~[[Category: Canonical catalytic triad]]~~	+
-	~~[[Category: Chymotrypsin-like]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: N-terminal beta-barrel~~]]	+

OCA at 09:27, 23 February 2022

OCA — Wed, 23 Feb 2022 09:27:36 GMT

←Older revision		Revision as of 09:27, 23 February 2022
Line 1:		Line 1:

	==Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)==		==Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)==
-	<StructureSection load='3fan' size='340' side='right' caption='[[3fan]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3fan' size='340' side='right'caption='[[3fan]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fan]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Prrsv Prrsv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FAN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3FAN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fan]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Prrsv Prrsv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FAN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mbm\|1mbm]], [[3fao\|3fao]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1mbm\|1mbm]], [[3fao\|3fao]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nsp4, ORF1a ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28344 PRRSV])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nsp4, ORF1a ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28344 PRRSV])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Equine_arterivirus_serine_peptidase Equine arterivirus serine peptidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.114 3.4.21.114] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Equine_arterivirus_serine_peptidase Equine arterivirus serine peptidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.114 3.4.21.114] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3fan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fan OCA], [~~http~~://pdbe.org/3fan PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3fan RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3fan PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3fan ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fan OCA], [https://pdbe.org/3fan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fan RCSB], [https://www.ebi.ac.uk/pdbsum/3fan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fan ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Equine arterivirus serine peptidase]]		[[Category: Equine arterivirus serine peptidase]]
		+	[[Category: Large Structures]]
	[[Category: Prrsv]]		[[Category: Prrsv]]
	[[Category: Gao, F]]		[[Category: Gao, F]]

OCA at 07:41, 5 December 2018

OCA — Wed, 05 Dec 2018 07:41:33 GMT

←Older revision		Revision as of 07:41, 5 December 2018
Line 1:		Line 1:
		+
	==Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)==		==Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)==
	<StructureSection load='3fan' size='340' side='right' caption='[[3fan]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3fan' size='340' side='right' caption='[[3fan]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nsp4, ORF1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28344 PRRSV])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nsp4, ORF1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28344 PRRSV])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Equine_arterivirus_serine_peptidase Equine arterivirus serine peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.114 3.4.21.114] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Equine_arterivirus_serine_peptidase Equine arterivirus serine peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.114 3.4.21.114] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fan OCA], [http://pdbe.org/3fan PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fan RCSB], [http://www.ebi.ac.uk/pdbsum/3fan PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fan OCA], [http://pdbe.org/3fan PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fan RCSB], [http://www.ebi.ac.uk/pdbsum/3fan PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fan ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fa/3fan_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fa/3fan_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 07:54, 9 February 2016

OCA — Tue, 09 Feb 2016 07:54:09 GMT

←Older revision		Revision as of 07:54, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3fan' size='340' side='right' caption='[[3fan]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3fan' size='340' side='right' caption='[[3fan]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fan]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Porcine_reproductive_and_respiratory_syndrome_virus Porcine reproductive and respiratory syndrome virus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FAN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fan]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Prrsv Prrsv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FAN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mbm\|1mbm]], [[3fao\|3fao]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mbm\|1mbm]], [[3fao\|3fao]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nsp4, ORF1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28344 ~~Porcine reproductive and respiratory syndrome virus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nsp4, ORF1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28344 PRRSV])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Equine_arterivirus_serine_peptidase Equine arterivirus serine peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.114 3.4.21.114] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Equine_arterivirus_serine_peptidase Equine arterivirus serine peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.114 3.4.21.114] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fan OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fan RCSB], [http://www.ebi.ac.uk/pdbsum/3fan PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fan OCA], [http://pdbe.org/3fan PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fan RCSB], [http://www.ebi.ac.uk/pdbsum/3fan PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fan ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3fan" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Equine arterivirus serine peptidase]]		[[Category: Equine arterivirus serine peptidase]]
-	[[Category: ~~Porcine reproductive and respiratory syndrome virus~~]]	+	[[Category: Prrsv]]
	[[Category: Gao, F]]		[[Category: Gao, F]]
	[[Category: Gao, G F]]		[[Category: Gao, G F]]

OCA at 08:57, 26 November 2014

OCA — Wed, 26 Nov 2014 08:57:06 GMT

←Older revision		Revision as of 08:57, 26 November 2014
Line 1:		Line 1:
-	[[~~Image:~~3fan.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)==
		+	<StructureSection load='3fan' size='340' side='right' caption='[[3fan]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3fan]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Porcine_reproductive_and_respiratory_syndrome_virus Porcine reproductive and respiratory syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FAN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mbm\|1mbm]], [[3fao\|3fao]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nsp4, ORF1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28344 Porcine reproductive and respiratory syndrome virus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Equine_arterivirus_serine_peptidase Equine arterivirus serine peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.114 3.4.21.114] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fan OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fan RCSB], [http://www.ebi.ac.uk/pdbsum/3fan PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fa/3fan_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Biogenesis and replication of the porcine reproductive and respiratory syndrome virus (PRRSV) include the crucial step of replicative polyprotein processing by self-encoded proteases. Whole genome bioinformatics analysis suggests that nonstructural protein 4 (nsp4) is a 3C-like serine protease (3CLSP), responsible for most of the nonstructural protein processing. The gene encoding this protease was cloned and expressed in Escherichia coli in order to confirm this prediction. The purified protein was crystallized, and the structure was solved at 1.9 A resolution. In addition, the crystal structure of the Ser118Ala mutant was determined at 2.0 A resolution. The monomeric enzyme folds into three domains, similar to that of the homologous protease of equine arteritis virus, which, like PRRSV, is a member of the family Arteriviridae in the order of Nidovirales. The active site of the PRRSV 3CLSP is located between domains I and II and harbors a canonical catalytic triad comprising Ser118, His39, and Asp64. The structure also shows an atypical oxyanion hole and a partially collapsed S1 specificity pocket. The proteolytic activity of the purified protein was assessed in vitro. Three sites joining nonstructural protein domains in the PRRSV replicative polyprotein are confirmed to be processed by the enzyme. Two of them, the nsp3/nsp4 and nsp11/nsp12 junctions, are shown to be cleaved in trans, while cis cleavage is demonstrated for the nsp4/nsp5 linker. Thus, we provide structural evidence as well as enzymatic proof of the nsp4 protein being a functional 3CLSP. We also show that the enzyme has a strong preference for glutamic acid at the P1 position of the substrate.

-	~~{{STRUCTURE_3fan\| PDB=3fan \| SCENE= }}~~	+	Structure and cleavage specificity of the chymotrypsin-like serine protease (3CLSP/nsp4) of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV).,Tian X, Lu G, Gao F, Peng H, Feng Y, Ma G, Bartlam M, Tian K, Yan J, Hilgenfeld R, Gao GF J Mol Biol. 2009 Oct 2;392(4):977-93. Epub 2009 Jul 29. PMID:19646449<ref>PMID:19646449</ref>

-	~~===Crystal structure of chymotrypsin-like protease~~/~~proteinase (3CLSP/Nsp4)~~ of ~~porcine reproductive and respiratory syndrome virus (PRRSV)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19646449}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3fan]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Porcine_reproductive_and_respiratory_syndrome_virus Porcine reproductive and respiratory syndrome virus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3FAN OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019646449<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Equine arterivirus serine peptidase]]		[[Category: Equine arterivirus serine peptidase]]
	[[Category: Porcine reproductive and respiratory syndrome virus]]		[[Category: Porcine reproductive and respiratory syndrome virus]]
-	[[Category: Gao, F.]]	+	[[Category: Gao, F]]
-	[[Category: Gao, G F.]]	+	[[Category: Gao, G F]]
-	[[Category: Liu, Y.]]	+	[[Category: Liu, Y]]
-	[[Category: Lu, G.]]	+	[[Category: Lu, G]]
-	[[Category: Peng, H.]]	+	[[Category: Peng, H]]
-	[[Category: Tian, X.]]	+	[[Category: Tian, X]]
	[[Category: C-terminal alpha-beta extra domain]]		[[Category: C-terminal alpha-beta extra domain]]
	[[Category: Canonical catalytic triad]]		[[Category: Canonical catalytic triad]]

OCA: Protected "3fan" [edit=sysop:move=sysop]

OCA — Wed, 13 Feb 2013 11:23:50 GMT

Protected "3fan" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:23, 13 February 2013

OCA at 11:23, 13 February 2013

OCA — Wed, 13 Feb 2013 11:23:48 GMT

←Older revision		Revision as of 11:23, 13 February 2013
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-	{{Seed}}
	[[Image:3fan.png\|left\|200px]]		[[Image:3fan.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3fan", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3fan\| PDB=3fan \| SCENE= }}		{{STRUCTURE_3fan\| PDB=3fan \| SCENE= }}

	===Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)===		===Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19646449}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19646449 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19646449}}		{{ABSTRACT_PUBMED_19646449}}

	==About this Structure==		==About this Structure==
-	~~3FAN~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Porcine_reproductive_and_respiratory_syndrome_virus Porcine reproductive and respiratory syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FAN OCA].	+	[[3fan]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Porcine_reproductive_and_respiratory_syndrome_virus Porcine reproductive and respiratory syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FAN OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19646449~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019646449</ref><references group="xtra"/>
	[[Category: Equine arterivirus serine peptidase]]		[[Category: Equine arterivirus serine peptidase]]
	[[Category: Porcine reproductive and respiratory syndrome virus]]		[[Category: Porcine reproductive and respiratory syndrome virus]]
Line 37:		Line 25:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: N-terminal beta-barrel]]		[[Category: N-terminal beta-barrel]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 21 09:52:22 2009''

OCA at 07:52, 21 October 2009

OCA — Wed, 21 Oct 2009 07:52:22 GMT

←Older revision		Revision as of 07:52, 21 October 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3fan.~~jpg~~\|left\|200px]]	+	[[Image:3fan.png\|left\|200px]]

	<!--		<!--
Line 38:		Line 38:
	[[Category: N-terminal beta-barrel]]		[[Category: N-terminal beta-barrel]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 9~~ 09:31:47 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 21 09:52:22 2009''

OCA at 06:31, 9 September 2009

OCA — Wed, 09 Sep 2009 06:31:51 GMT

←Older revision		Revision as of 06:31, 9 September 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3fan.jpg\|left\|200px]]

-	The ~~entry 3fan~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3fan", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3fan\| PDB=3fan \| SCENE= }}

-	~~Authors: Gao, F., Peng, H., Tian, X., Lu, G., Liu, Y., Gao, G.F.~~	+	===Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)===

-	Description: Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Sep ~~3 15~~:16:56 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19646449}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19646449 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19646449}}
		+
		+	==About this Structure==
		+	3FAN is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Porcine_reproductive_and_respiratory_syndrome_virus Porcine reproductive and respiratory syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FAN OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19646449</ref><references group="xtra"/>
		+	[[Category: Equine arterivirus serine peptidase]]
		+	[[Category: Porcine reproductive and respiratory syndrome virus]]
		+	[[Category: Gao, F.]]
		+	[[Category: Gao, G F.]]
		+	[[Category: Liu, Y.]]
		+	[[Category: Lu, G.]]
		+	[[Category: Peng, H.]]
		+	[[Category: Tian, X.]]
		+	[[Category: C-terminal alpha-beta extra domain]]
		+	[[Category: Canonical catalytic triad]]
		+	[[Category: Chymotrypsin-like]]
		+	[[Category: Hydrolase]]
		+	[[Category: N-terminal beta-barrel]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 9 09:31:47 2009''

OCA at 12:16, 3 September 2009

OCA — Thu, 03 Sep 2009 12:16:56 GMT

←Older revision		Revision as of 12:16, 3 September 2009
Line 3:		Line 3:
	The entry 3fan is ON HOLD until Paper Publication		The entry 3fan is ON HOLD until Paper Publication

-	Authors: Tian, X., Lu, G., ~~Peng~~, ~~H., Gao, F~~., Gao, G.F.	+	Authors: Gao, F., Peng, H., Tian, X., Lu, G., Liu, Y., Gao, G.F.

	Description: Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)		Description: Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Dec 10 14~~:40:~~22 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 15:16:56 2009''