3fgc - Revision history

OCA at 06:46, 6 September 2023

OCA — Wed, 06 Sep 2023 06:46:00 GMT

←Older revision		Revision as of 06:46, 6 September 2023
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	<StructureSection load='3fgc' size='340' side='right'caption='[[3fgc]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3fgc' size='340' side='right'caption='[[3fgc]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fgc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"achromobacter_harveyi"_johnson_and_shunk_1936 "achromobacter~~ harveyi~~" johnson and shunk 1936~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fgc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FGC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>~~, ~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1luc\|1luc]], [[1brl\|1brl]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>luxA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~669~~ "~~Achromobacter harveyi~~" ~~Johnson and Shunk 1936]), luxB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=669 "Achromobacter harveyi" Johnson and Shunk 1936])</td~~></tr>	+
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgc OCA], [https://pdbe.org/3fgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fgc RCSB], [https://www.ebi.ac.uk/pdbsum/3fgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fgc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgc OCA], [https://pdbe.org/3fgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fgc RCSB], [https://www.ebi.ac.uk/pdbsum/3fgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fgc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LUXA_VIBHA LUXA_VIBHA]] Light-emitting reaction in luminous bacteria. [[https://www.uniprot.org/uniprot/LUXB_VIBHA LUXB_VIBHA]] Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.	+	[https://www.uniprot.org/uniprot/LUXA_VIBHA LUXA_VIBHA] Light-emitting reaction in luminous bacteria.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Achromobacter harveyi johnson and shunk 1936]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Baldwin, T O~~]]	+	[[Category: Vibrio harveyi]]
-	[[Category: ~~Campbell, Z T~~]]	+	[[Category: Baldwin TO]]
-	[[Category: ~~Montfort, W R~~]]	+	[[Category: Campbell ZT]]
-	[[Category: ~~Weichsel, A~~]]	+	[[Category: Montfort WR]]
-	[[Category: ~~Flavoprotein]]~~	+	[[Category: Weichsel A]]
-	~~[[Category: Fmn]]~~	+
-	~~[[Category: Fmn bacterial luciferase mobile loop]]~~	+
-	~~[[Category: Luminescence]]~~	+
-	~~[[Category: Monooxygenase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Photoprotein~~]]	+

OCA at 08:00, 2 March 2022

OCA — Wed, 02 Mar 2022 08:00:50 GMT

←Older revision		Revision as of 08:00, 2 March 2022
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	==Crystal Structure of the Bacterial Luciferase:Flavin Complex Reveals the Basis of Intersubunit Communication==		==Crystal Structure of the Bacterial Luciferase:Flavin Complex Reveals the Basis of Intersubunit Communication==
-	<StructureSection load='3fgc' size='340' side='right' caption='[[3fgc]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3fgc' size='340' side='right'caption='[[3fgc]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fgc]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"achromobacter_harveyi"_johnson_and_shunk_1936 "achromobacter harveyi" johnson and shunk 1936]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3FGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fgc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"achromobacter_harveyi"_johnson_and_shunk_1936 "achromobacter harveyi" johnson and shunk 1936]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FGC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1luc\|1luc]], [[1brl\|1brl]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1luc\|1luc]], [[1brl\|1brl]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">luxA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 "Achromobacter harveyi" Johnson and Shunk 1936]), luxB ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 "Achromobacter harveyi" Johnson and Shunk 1936])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">luxA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 "Achromobacter harveyi" Johnson and Shunk 1936]), luxB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 "Achromobacter harveyi" Johnson and Shunk 1936])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3fgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgc OCA], [~~http~~://pdbe.org/3fgc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3fgc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3fgc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3fgc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgc OCA], [https://pdbe.org/3fgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fgc RCSB], [https://www.ebi.ac.uk/pdbsum/3fgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fgc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LUXA_VIBHA LUXA_VIBHA]] Light-emitting reaction in luminous bacteria. [[~~http~~://www.uniprot.org/uniprot/LUXB_VIBHA LUXB_VIBHA]] Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.	+	[[https://www.uniprot.org/uniprot/LUXA_VIBHA LUXA_VIBHA]] Light-emitting reaction in luminous bacteria. [[https://www.uniprot.org/uniprot/LUXB_VIBHA LUXB_VIBHA]] Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/3fgc_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/3fgc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 33:

	==See Also==		==See Also==
-	*[[Luciferase ~~FMN complex- Vibrio harveyi~~\|Luciferase ~~FMN complex- Vibrio harveyi~~]]	+	*[[Luciferase 3D structures\|Luciferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Achromobacter harveyi johnson and shunk 1936]]		[[Category: Achromobacter harveyi johnson and shunk 1936]]
		+	[[Category: Large Structures]]
	[[Category: Baldwin, T O]]		[[Category: Baldwin, T O]]
	[[Category: Campbell, Z T]]		[[Category: Campbell, Z T]]

OCA at 06:41, 1 November 2017

OCA — Wed, 01 Nov 2017 06:41:53 GMT

←Older revision		Revision as of 06:41, 1 November 2017
Line 1:		Line 1:
		+
	==Crystal Structure of the Bacterial Luciferase:Flavin Complex Reveals the Basis of Intersubunit Communication==		==Crystal Structure of the Bacterial Luciferase:Flavin Complex Reveals the Basis of Intersubunit Communication==
	<StructureSection load='3fgc' size='340' side='right' caption='[[3fgc]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3fgc' size='340' side='right' caption='[[3fgc]], [[Resolution\|resolution]] 2.30Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">luxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 "Achromobacter harveyi" Johnson and Shunk 1936]), luxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 "Achromobacter harveyi" Johnson and Shunk 1936])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">luxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 "Achromobacter harveyi" Johnson and Shunk 1936]), luxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 "Achromobacter harveyi" Johnson and Shunk 1936])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgc OCA], [http://pdbe.org/3fgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fgc RCSB], [http://www.ebi.ac.uk/pdbsum/3fgc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgc OCA], [http://pdbe.org/3fgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fgc RCSB], [http://www.ebi.ac.uk/pdbsum/3fgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fgc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 32:		Line 33:

	==See Also==		==See Also==
-	*[[Luciferase\|Luciferase]]
	*[[Luciferase FMN complex- Vibrio harveyi\|Luciferase FMN complex- Vibrio harveyi]]		*[[Luciferase FMN complex- Vibrio harveyi\|Luciferase FMN complex- Vibrio harveyi]]
	== References ==		== References ==

OCA at 13:57, 7 February 2016

OCA — Sun, 07 Feb 2016 13:57:34 GMT

←Older revision		Revision as of 13:57, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='3fgc' size='340' side='right' caption='[[3fgc]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3fgc' size='340' side='right' caption='[[3fgc]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fgc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Vibrio_harveyi Vibrio~~ harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fgc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_harveyi"_johnson_and_shunk_1936 "achromobacter harveyi" johnson and shunk 1936]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FGC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1luc\|1luc]], [[1brl\|1brl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1luc\|1luc]], [[1brl\|1brl]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">luxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 ~~Vibrio~~ harveyi]), luxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 ~~Vibrio~~ harveyi])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">luxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 "Achromobacter harveyi" Johnson and Shunk 1936]), luxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 "Achromobacter harveyi" Johnson and Shunk 1936])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fgc RCSB], [http://www.ebi.ac.uk/pdbsum/3fgc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgc OCA], [http://pdbe.org/3fgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fgc RCSB], [http://www.ebi.ac.uk/pdbsum/3fgc PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fgc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3fgc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio~~ harveyi]]	+	[[Category: Achromobacter harveyi johnson and shunk 1936]]
	[[Category: Baldwin, T O]]		[[Category: Baldwin, T O]]
	[[Category: Campbell, Z T]]		[[Category: Campbell, Z T]]

OCA at 15:41, 24 December 2014

OCA — Wed, 24 Dec 2014 15:41:07 GMT

←Older revision		Revision as of 15:41, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fgc RCSB], [http://www.ebi.ac.uk/pdbsum/3fgc PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fgc RCSB], [http://www.ebi.ac.uk/pdbsum/3fgc PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LUXA_VIBHA LUXA_VIBHA]] Light-emitting reaction in luminous bacteria. [[http://www.uniprot.org/uniprot/LUXB_VIBHA LUXB_VIBHA]] Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 14:13, 17 December 2014

OCA — Wed, 17 Dec 2014 14:13:50 GMT

←Older revision		Revision as of 14:13, 17 December 2014
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-	~~{{STRUCTURE_3fgc\| PDB=3fgc \| SCENE= }}~~	+	==Crystal Structure of the Bacterial Luciferase:Flavin Complex Reveals the Basis of Intersubunit Communication==
-	===Crystal Structure of the Bacterial Luciferase:Flavin Complex Reveals the Basis of Intersubunit Communication===	+	<StructureSection load='3fgc' size='340' side='right' caption='[[3fgc]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19435287}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3fgc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FGC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1luc\|1luc]], [[1brl\|1brl]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">luxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 Vibrio harveyi]), luxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 Vibrio harveyi])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fgc RCSB], [http://www.ebi.ac.uk/pdbsum/3fgc PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/3fgc_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial luciferase from <i>V. harveyi</i> is a heterodimer composed of a catalytic alpha subunit and an homologous but non-catalytic beta subunit. Despite decades of enzymological investigation, structural evidence defining the active center has been elusive. We report here the crystal structure of <i>V. harveyi</i> luciferase bound to FMN at 2.3 A. The isoalloxazine ring is coordinated by an unusual <i>cis</i> Ala-Ala peptide bond. The reactive sulfhydryl group of Cys 106 projects toward position C-4a, the site of flavin-oxygenation. This structure also provides the first data specifying the conformations of a mobile loop that is crystallographically disordered in both prior crystal structures [Fisher, A. J., Raushel, F. M., Baldwin, T. O., & Rayment, I. (1995) Biochemistry 34, 6581-6586; Fisher, A. J., Thompson, T. B., Thoden, J. B., Baldwin, T. O., & Rayment, I. (1996) J. Biol. Chem. 271, 21956-21968]. This loop appears to be a boundary between solvent and the active center. Within this portion of the protein, a single contact was observed between Phe 272 of the alpha subunit, not seen in the previous structures, and Tyr 151 of the beta subunit. Substitutions at position 151 on the beta subunit caused reductions in activity and total quantum yield. Several of these mutants were found to have decreased affinity for FMNH2. These findings partially address the long-standing question of how the beta subunit stabilizes the active conformation of the alpha subunit, thereby participating in the catalytic mechanism.

-	~~==About this~~ Structure==	+	Crystal Structure of the Bacterial Luciferase:Flavin Complex Provides Insight into the Function of the subunit.,Campbell ZT, Weichsel A, Montfort WR, Baldwin TO Biochemistry. 2009 May 12. PMID:19435287<ref>PMID:19435287</ref>
-	~~[[3fgc]] is a 4 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Vibrio_harveyi Vibrio harveyi]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3FGC OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Luciferase\|Luciferase]]		*[[Luciferase\|Luciferase]]
	*[[Luciferase FMN complex- Vibrio harveyi\|Luciferase FMN complex- Vibrio harveyi]]		*[[Luciferase FMN complex- Vibrio harveyi\|Luciferase FMN complex- Vibrio harveyi]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019435287<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Vibrio harveyi]]		[[Category: Vibrio harveyi]]
-	[[Category: Baldwin, T O.]]	+	[[Category: Baldwin, T O]]
-	[[Category: Campbell, Z T.]]	+	[[Category: Campbell, Z T]]
-	[[Category: Montfort, W R.]]	+	[[Category: Montfort, W R]]
-	[[Category: Weichsel, A.]]	+	[[Category: Weichsel, A]]
	[[Category: Flavoprotein]]		[[Category: Flavoprotein]]
	[[Category: Fmn]]		[[Category: Fmn]]

OCA at 10:09, 11 March 2013

OCA — Mon, 11 Mar 2013 10:09:45 GMT

←Older revision		Revision as of 10:09, 11 March 2013
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-	[[Image:3fgc.png\|left\|200px]]
-
	{{STRUCTURE_3fgc\| PDB=3fgc \| SCENE= }}		{{STRUCTURE_3fgc\| PDB=3fgc \| SCENE= }}
-
	===Crystal Structure of the Bacterial Luciferase:Flavin Complex Reveals the Basis of Intersubunit Communication===		===Crystal Structure of the Bacterial Luciferase:Flavin Complex Reveals the Basis of Intersubunit Communication===
-
	{{ABSTRACT_PUBMED_19435287}}		{{ABSTRACT_PUBMED_19435287}}

OCA at 06:50, 20 October 2012

OCA — Sat, 20 Oct 2012 06:50:48 GMT

←Older revision		Revision as of 06:50, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3fgc]] is a 4 chain structure ~~of [[Luciferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGC OCA].	+	[[3fgc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGC OCA].

	==See Also==		==See Also==

OCA: Protected "3fgc" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 14:29:39 GMT

Protected "3fgc" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:29, 25 July 2012

OCA at 14:29, 25 July 2012

OCA — Wed, 25 Jul 2012 14:29:37 GMT

←Older revision		Revision as of 14:29, 25 July 2012
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-	{{Seed}}
	[[Image:3fgc.png\|left\|200px]]		[[Image:3fgc.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3fgc\| PDB=3fgc \| SCENE= }}		{{STRUCTURE_3fgc\| PDB=3fgc \| SCENE= }}

	===Crystal Structure of the Bacterial Luciferase:Flavin Complex Reveals the Basis of Intersubunit Communication===		===Crystal Structure of the Bacterial Luciferase:Flavin Complex Reveals the Basis of Intersubunit Communication===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19435287}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19435287 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_19435287}}		{{ABSTRACT_PUBMED_19435287}}

	==About this Structure==		==About this Structure==
-	~~3FGC~~ is a 4 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGC OCA].	+	[[3fgc]] is a 4 chain structure of [[Luciferase]] with sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGC OCA].
		+
		+	==See Also==
		+	*[[Luciferase\|Luciferase]]
		+	*[[Luciferase FMN complex- Vibrio harveyi\|Luciferase FMN complex- Vibrio harveyi]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19435287~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019435287</ref><references group="xtra"/>
	[[Category: Vibrio harveyi]]		[[Category: Vibrio harveyi]]
	[[Category: Baldwin, T O.]]		[[Category: Baldwin, T O.]]
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	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Photoprotein]]		[[Category: Photoprotein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 15 14:00:48 2009''