3fjm - Revision history

OCA at 06:47, 6 September 2023

OCA — Wed, 06 Sep 2023 06:47:09 GMT

←Older revision		Revision as of 06:47, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3fjm' size='340' side='right'caption='[[3fjm]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='3fjm' size='340' side='right'caption='[[3fjm]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fjm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp.~~ jejuni~~" smibert 1974~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FJM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FJM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fjm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FJM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FJM FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[2hxw\|2hxw]], [[3fir\|3fir]], [[3fj7\|3fj7]], [[3fjg\|3fjg]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">Cj0289c, peb3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fjm OCA], [https://pdbe.org/3fjm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fjm RCSB], [https://www.ebi.ac.uk/pdbsum/3fjm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fjm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fjm OCA], [https://pdbe.org/3fjm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fjm RCSB], [https://www.ebi.ac.uk/pdbsum/3fjm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fjm ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q0PBL7_CAMJE Q0PBL7_CAMJE]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Campylobacter ~~fetus subsp.~~ jejuni ~~smibert 1974~~]]	+	[[Category: Campylobacter jejuni]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cygler, M]]	+	[[Category: Cygler M]]
-	[[Category: Matte, A]]	+	[[Category: Matte A]]
-	[[Category: Min, T]]	+	[[Category: Min T]]
-	~~[[Category: Peb3]]~~	+
-	~~[[Category: Phosphate. crystal structure]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 08:02, 2 March 2022

OCA — Wed, 02 Mar 2022 08:02:50 GMT

←Older revision		Revision as of 08:02, 2 March 2022
Line 1:		Line 1:

	==crystal structure of phosphate bound PEB3==		==crystal structure of phosphate bound PEB3==
-	<StructureSection load='3fjm' size='340' side='right' caption='[[3fjm]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='3fjm' size='340' side='right'caption='[[3fjm]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fjm]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FJM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3FJM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fjm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FJM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FJM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hxw\|2hxw]], [[3fir\|3fir]], [[3fj7\|3fj7]], [[3fjg\|3fjg]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2hxw\|2hxw]], [[3fir\|3fir]], [[3fj7\|3fj7]], [[3fjg\|3fjg]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cj0289c, peb3 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cj0289c, peb3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3fjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fjm OCA], [~~http~~://pdbe.org/3fjm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3fjm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3fjm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3fjm ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fjm OCA], [https://pdbe.org/3fjm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fjm RCSB], [https://www.ebi.ac.uk/pdbsum/3fjm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fjm ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Campylobacter fetus subsp. jejuni smibert 1974]]		[[Category: Campylobacter fetus subsp. jejuni smibert 1974]]
		+	[[Category: Large Structures]]
	[[Category: Cygler, M]]		[[Category: Cygler, M]]
	[[Category: Matte, A]]		[[Category: Matte, A]]

OCA at 07:27, 5 December 2018

OCA — Wed, 05 Dec 2018 07:27:20 GMT

←Older revision		Revision as of 07:27, 5 December 2018
Line 1:		Line 1:
		+
	==crystal structure of phosphate bound PEB3==		==crystal structure of phosphate bound PEB3==
	<StructureSection load='3fjm' size='340' side='right' caption='[[3fjm]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='3fjm' size='340' side='right' caption='[[3fjm]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hxw\|2hxw]], [[3fir\|3fir]], [[3fj7\|3fj7]], [[3fjg\|3fjg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hxw\|2hxw]], [[3fir\|3fir]], [[3fj7\|3fj7]], [[3fjg\|3fjg]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cj0289c, peb3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cj0289c, peb3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fjm OCA], [http://pdbe.org/3fjm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fjm RCSB], [http://www.ebi.ac.uk/pdbsum/3fjm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fjm OCA], [http://pdbe.org/3fjm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fjm RCSB], [http://www.ebi.ac.uk/pdbsum/3fjm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fjm ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/3fjm_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/3fjm_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 12:51, 9 February 2016

OCA — Tue, 09 Feb 2016 12:51:41 GMT

←Older revision		Revision as of 12:51, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3fjm' size='340' side='right' caption='[[3fjm]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='3fjm' size='340' side='right' caption='[[3fjm]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fjm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Campylobacter_jejuni Campylobacter~~ jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FJM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FJM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fjm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FJM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FJM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hxw\|2hxw]], [[3fir\|3fir]], [[3fj7\|3fj7]], [[3fjg\|3fjg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hxw\|2hxw]], [[3fir\|3fir]], [[3fj7\|3fj7]], [[3fjg\|3fjg]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cj0289c, peb3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 Campylobacter jejuni])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cj0289c, peb3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fjm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fjm RCSB], [http://www.ebi.ac.uk/pdbsum/3fjm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fjm OCA], [http://pdbe.org/3fjm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fjm RCSB], [http://www.ebi.ac.uk/pdbsum/3fjm PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fjm ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3fjm" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Campylobacter jejuni]]	+	[[Category: Campylobacter fetus subsp. jejuni smibert 1974]]
	[[Category: Cygler, M]]		[[Category: Cygler, M]]
	[[Category: Matte, A]]		[[Category: Matte, A]]

OCA at 10:15, 3 December 2014

OCA — Wed, 03 Dec 2014 10:15:27 GMT

←Older revision		Revision as of 10:15, 3 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3fjm.~~png~~\|~~left~~\|200px]]	+	==crystal structure of phosphate bound PEB3==
		+	<StructureSection load='3fjm' size='340' side='right' caption='[[3fjm]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3fjm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FJM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FJM FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hxw\|2hxw]], [[3fir\|3fir]], [[3fj7\|3fj7]], [[3fjg\|3fjg]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cj0289c, peb3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 Campylobacter jejuni])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fjm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fjm RCSB], [http://www.ebi.ac.uk/pdbsum/3fjm PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/3fjm_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	PEB3 is a glycoprotein adhesin from C. jejuni whose structure suggested a role in transport. We have investigated potential ligands for PEB3 and characterized their binding properties using biophysical methods in solution and by X-ray crystallography. A thermal aggregation assay of PEB3 with a library of physiological compounds identified three possible ligands (3-phosphoglycerate (3-PG), phosphoenolpyruvate (PEP) and aconitate), which stabilized wild type PEB3 but did not stabilize either a PEB3 form containing two mutations at the ligand-binding site, T138A/S139A, or a second PEB3 mutant, K135E, at a site ~14 A away. Fluorescence titration experiments and co-crystal structures with various ligands were used to characterize the binding of 3-PG, PEP and phosphate to PEB3. Further, a C. jejuni growth experiment in minimal medium supplemented with 3-PG showed that this molecule enhances the growth of wild type C. jejuni, but not of the PEB3 mutants. Crystallographic analysis of PEB3 complexes revealed that the region Ser171-Gln180, in the presence of 3-PG, or other phosphates, is helical and similar to other transport proteins, but it is non-helical when citrate is bound. The K135E mutation resulted in expression of a more highly glycosylated form of PEB3 in vivo and its crystal structure showed the conformation of the first two residues of the glycan. Based on our findings, we suggest that PEB3 is a transport protein that may function in utilization of 3-PG or other phosphate-containing molecules from the host.

-	~~{{STRUCTURE_3fjm\| PDB=3fjm \| SCENE= }}~~	+	Specificity of the Campylobacter jejuni Adhesin PEB3 for Phosphates and Structural Differences among its Ligand Complexes.,Min T, Vedadi M, Watson D, Wasney G, Munger C, Cygler M, Matte A, Young N Biochemistry. 2009 Feb 23. PMID:19236052<ref>PMID:19236052</ref>

-	~~===crystal structure~~ of ~~phosphate bound PEB3===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19236052}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3fjm]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Campylobacter_jejuni Campylobacter jejuni]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3FJM OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019236052<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Campylobacter jejuni]]		[[Category: Campylobacter jejuni]]
-	[[Category: Cygler, M.]]	+	[[Category: Cygler, M]]
-	[[Category: Matte, A.]]	+	[[Category: Matte, A]]
-	[[Category: Min, T.]]	+	[[Category: Min, T]]
	[[Category: Peb3]]		[[Category: Peb3]]
	[[Category: Phosphate. crystal structure]]		[[Category: Phosphate. crystal structure]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA: Protected "3fjm" [edit=sysop:move=sysop]

OCA — Wed, 13 Feb 2013 11:06:30 GMT

Protected "3fjm" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:06, 13 February 2013

OCA at 11:06, 13 February 2013

OCA — Wed, 13 Feb 2013 11:06:27 GMT

←Older revision		Revision as of 11:06, 13 February 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3fjm.png\|left\|200px]]		[[Image:3fjm.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3fjm", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3fjm\| PDB=3fjm \| SCENE= }}		{{STRUCTURE_3fjm\| PDB=3fjm \| SCENE= }}

	===crystal structure of phosphate bound PEB3===		===crystal structure of phosphate bound PEB3===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19236052}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19236052 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19236052}}		{{ABSTRACT_PUBMED_19236052}}

	==About this Structure==		==About this Structure==
-	~~3FJM~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FJM OCA].	+	[[3fjm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FJM OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19236052~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019236052</ref><references group="xtra"/>
	[[Category: Campylobacter jejuni]]		[[Category: Campylobacter jejuni]]
	[[Category: Cygler, M.]]		[[Category: Cygler, M.]]
Line 31:		Line 19:
	[[Category: Phosphate. crystal structure]]		[[Category: Phosphate. crystal structure]]
	[[Category: Transport protein]]		[[Category: Transport protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 22 10:57:49 2009''

OCA at 07:57, 22 April 2009

OCA — Wed, 22 Apr 2009 07:57:49 GMT

←Older revision		Revision as of 07:57, 22 April 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3fjm.~~jpg~~\|left\|200px]]	+	[[Image:3fjm.png\|left\|200px]]

	<!--		<!--
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	[[Category: Transport protein]]		[[Category: Transport protein]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar 11 11~~:25:39 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 22 10:57:49 2009''

OCA at 09:25, 11 March 2009

OCA — Wed, 11 Mar 2009 09:25:40 GMT

←Older revision		Revision as of 09:25, 11 March 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3fjm.jpg\|left\|200px]]

-	The ~~entry 3fjm~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3fjm", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3fjm\| PDB=3fjm \| SCENE= }}

-	~~Authors: Min, T., Matte, A., Cygler, M.~~	+	===crystal structure of phosphate bound PEB3===

-	Description: crystal structure of phosphate bound PEB3

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Feb~~ 11 12:35:18 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19236052}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19236052 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19236052}}
		+
		+	==About this Structure==
		+	3FJM is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FJM OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19236052</ref><references group="xtra"/>
		+	[[Category: Campylobacter jejuni]]
		+	[[Category: Cygler, M.]]
		+	[[Category: Matte, A.]]
		+	[[Category: Min, T.]]
		+	[[Category: Peb3]]
		+	[[Category: Phosphate. crystal structure]]
		+	[[Category: Transport protein]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 11 11:25:39 2009''

OCA at 10:35, 11 February 2009

OCA — Wed, 11 Feb 2009 10:35:18 GMT

←Older revision		Revision as of 10:35, 11 February 2009
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3fjm is ON HOLD	+	The entry 3fjm is ON HOLD until Paper Publication

	Authors: Min, T., Matte, A., Cygler, M.		Authors: Min, T., Matte, A., Cygler, M.
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	Description: crystal structure of phosphate bound PEB3		Description: crystal structure of phosphate bound PEB3

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Dec 31 09~~:48:~~53 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 11 12:35:18 2009''