3fs8 - Revision history

OCA at 09:50, 21 February 2024

OCA — Wed, 21 Feb 2024 09:50:46 GMT

←Older revision		Revision as of 09:50, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3fs8' size='340' side='right'caption='[[3fs8]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3fs8' size='340' side='right'caption='[[3fs8]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fs8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"clostridium_tartarivorum"_mercer_and_vaughn "clostridium tartarivorum" mercer and vaughn~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FS8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fs8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FS8 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>~~, ~~<scene name='pdbligand=TDR:THYMINE'>TDR</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3fsb\|3fsb]], [[3fsc\|3fsc]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=TDR:THYMINE'>TDR</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">qdtC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1517 "Clostridium tartarivorum" Mercer and Vaughn])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fs8 OCA], [https://pdbe.org/3fs8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fs8 RCSB], [https://www.ebi.ac.uk/pdbsum/3fs8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fs8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fs8 OCA], [https://pdbe.org/3fs8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fs8 RCSB], [https://www.ebi.ac.uk/pdbsum/3fs8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fs8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6TFC6_THETR Q6TFC6_THETR]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fs8 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fs8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	3-acetamido-3,6-didexoy-alpha-D-glucose or Quip3NAc is an unusual dideoxy sugar found in the O-antigens of various Gram-negative bacteria and in the S-layer glycoprotein glycans of some Gram-positive bacteria. It is produced in these organisms as a dTDP-linked sugar, with five enzymes ultimately required for its biosynthesis. The focus of this investigation is on the enzyme QdtC, a CoA-dependent N-acetyltransferase that catalyzes the last step in the Quip3NAc biosynthetic pathway. For this analysis, three crystal structures were determined: the wild-type enzyme in the presence of acetyl-CoA, and two ternary complexes of the enzyme with CoA and either dTDP-D-Quip3NAc or dTDP-3-amino-3,6-didexoy-alphalpha-D-galactose (dTDP-D-Fucp3N). Each subunit of the trimeric enzyme is dominated by a left-handed beta-helix motif with 11 turns. The three active sites are located at the subunit:subunit interfaces, and the two dTDP-sugar ligands employed in this study bind to the protein in nearly identical manners. Those residues responsible for anchoring the hexose moieties of the dTDP-sugars to the protein include Glu 141, Asn 159, Asp 160 from one subunit and His 134 from another subunit. To probe the roles of various amino acid residues in the catalytic mechanism of the enzyme, ten site-directed mutant proteins were constructed and their kinetic parameters measured. On the basis of these data, a catalytic mechanism is proposed for QdtC whereby the acetylation of the sugar amino group does not require a catalytic base provided by the protein. Rather, the sulfur of CoA functions as the ultimate proton acceptor.
-
-	Structural and Functional Studies of QdtC: an N-Acetyltransferase Required for the Biosynthesis of dTDP-3-Acetamido-3,6-Dideoxy--D-Glucose.,Thoden J, Cook P, Schaffer C, Messner P, Holden H Biochemistry. 2009 Feb 4. PMID:19191736<ref>PMID:19191736</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3fs8" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Clostridium tartarivorum mercer and vaughn]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Holden, H M~~]]	+	[[Category: Thermoanaerobacterium thermosaccharolyticum]]
-	[[Category: ~~Thoden, J B~~]]	+	[[Category: Holden HM]]
-	[[Category: ~~Acetyltransferase]]~~	+	[[Category: Thoden JB]]
-	~~[[Category: Deoxysugar]]~~	+
-	~~[[Category: Natural product]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:09, 2 March 2022

OCA — Wed, 02 Mar 2022 08:09:30 GMT

←Older revision		Revision as of 08:09, 2 March 2022
Line 1:		Line 1:

	==Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA==		==Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA==
-	<StructureSection load='3fs8' size='340' side='right' caption='[[3fs8]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='3fs8' size='340' side='right'caption='[[3fs8]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fs8]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"clostridium_tartarivorum"_mercer_and_vaughn "clostridium tartarivorum" mercer and vaughn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3FS8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fs8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"clostridium_tartarivorum"_mercer_and_vaughn "clostridium tartarivorum" mercer and vaughn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FS8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=TDR:THYMINE'>TDR</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=TDR:THYMINE'>TDR</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fsb\|3fsb]], [[3fsc\|3fsc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3fsb\|3fsb]], [[3fsc\|3fsc]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">qdtC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1517 "Clostridium tartarivorum" Mercer and Vaughn])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">qdtC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1517 "Clostridium tartarivorum" Mercer and Vaughn])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3fs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fs8 OCA], [~~http~~://pdbe.org/3fs8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3fs8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3fs8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3fs8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fs8 OCA], [https://pdbe.org/3fs8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fs8 RCSB], [https://www.ebi.ac.uk/pdbsum/3fs8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fs8 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Clostridium tartarivorum mercer and vaughn]]		[[Category: Clostridium tartarivorum mercer and vaughn]]
		+	[[Category: Large Structures]]
	[[Category: Holden, H M]]		[[Category: Holden, H M]]
	[[Category: Thoden, J B]]		[[Category: Thoden, J B]]

OCA at 07:56, 5 December 2018

OCA — Wed, 05 Dec 2018 07:56:14 GMT

←Older revision		Revision as of 07:56, 5 December 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/3fs8_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/3fs8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:26, 9 December 2016

OCA — Fri, 09 Dec 2016 20:26:44 GMT

←Older revision		Revision as of 20:26, 9 December 2016
Line 1:		Line 1:
		+
	==Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA==		==Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA==
	<StructureSection load='3fs8' size='340' side='right' caption='[[3fs8]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3fs8' size='340' side='right' caption='[[3fs8]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3fs8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FS8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3fs8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_tartarivorum"_mercer_and_vaughn "clostridium tartarivorum" mercer and vaughn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FS8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=TDR:THYMINE'>TDR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=TDR:THYMINE'>TDR</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fsb\|3fsb]], [[3fsc\|3fsc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fsb\|3fsb]], [[3fsc\|3fsc]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">qdtC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1517 ~~Thermoanaerobacterium thermosaccharolyticum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">qdtC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1517 "Clostridium tartarivorum" Mercer and Vaughn])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fs8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fs8 RCSB], [http://www.ebi.ac.uk/pdbsum/3fs8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fs8 OCA], [http://pdbe.org/3fs8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fs8 RCSB], [http://www.ebi.ac.uk/pdbsum/3fs8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fs8 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fs8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3fs8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thermoanaerobacterium thermosaccharolyticum~~]]	+	[[Category: Clostridium tartarivorum mercer and vaughn]]
	[[Category: Holden, H M]]		[[Category: Holden, H M]]
	[[Category: Thoden, J B]]		[[Category: Thoden, J B]]

OCA at 10:15, 3 December 2014

OCA — Wed, 03 Dec 2014 10:15:40 GMT

←Older revision		Revision as of 10:15, 3 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3fs8.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA==
		+	<StructureSection load='3fs8' size='340' side='right' caption='[[3fs8]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3fs8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FS8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=TDR:THYMINE'>TDR</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fsb\|3fsb]], [[3fsc\|3fsc]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">qdtC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1517 Thermoanaerobacterium thermosaccharolyticum])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fs8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fs8 RCSB], [http://www.ebi.ac.uk/pdbsum/3fs8 PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/3fs8_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	3-acetamido-3,6-didexoy-alpha-D-glucose or Quip3NAc is an unusual dideoxy sugar found in the O-antigens of various Gram-negative bacteria and in the S-layer glycoprotein glycans of some Gram-positive bacteria. It is produced in these organisms as a dTDP-linked sugar, with five enzymes ultimately required for its biosynthesis. The focus of this investigation is on the enzyme QdtC, a CoA-dependent N-acetyltransferase that catalyzes the last step in the Quip3NAc biosynthetic pathway. For this analysis, three crystal structures were determined: the wild-type enzyme in the presence of acetyl-CoA, and two ternary complexes of the enzyme with CoA and either dTDP-D-Quip3NAc or dTDP-3-amino-3,6-didexoy-alphalpha-D-galactose (dTDP-D-Fucp3N). Each subunit of the trimeric enzyme is dominated by a left-handed beta-helix motif with 11 turns. The three active sites are located at the subunit:subunit interfaces, and the two dTDP-sugar ligands employed in this study bind to the protein in nearly identical manners. Those residues responsible for anchoring the hexose moieties of the dTDP-sugars to the protein include Glu 141, Asn 159, Asp 160 from one subunit and His 134 from another subunit. To probe the roles of various amino acid residues in the catalytic mechanism of the enzyme, ten site-directed mutant proteins were constructed and their kinetic parameters measured. On the basis of these data, a catalytic mechanism is proposed for QdtC whereby the acetylation of the sugar amino group does not require a catalytic base provided by the protein. Rather, the sulfur of CoA functions as the ultimate proton acceptor.

-	~~{{STRUCTURE_3fs8\| PDB=3fs8 \| SCENE= }}~~	+	Structural and Functional Studies of QdtC: an N-Acetyltransferase Required for the Biosynthesis of dTDP-3-Acetamido-3,6-Dideoxy--D-Glucose.,Thoden J, Cook P, Schaffer C, Messner P, Holden H Biochemistry. 2009 Feb 4. PMID:19191736<ref>PMID:19191736</ref>

-	~~===Crystal structure~~ of ~~QdtC,~~ the ~~dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19191736}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3fs8]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019191736<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Thermoanaerobacterium thermosaccharolyticum]]		[[Category: Thermoanaerobacterium thermosaccharolyticum]]
-	[[Category: Holden, H M.]]	+	[[Category: Holden, H M]]
-	[[Category: Thoden, J B.]]	+	[[Category: Thoden, J B]]
	[[Category: Acetyltransferase]]		[[Category: Acetyltransferase]]
	[[Category: Deoxysugar]]		[[Category: Deoxysugar]]
	[[Category: Natural product]]		[[Category: Natural product]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA: Protected "3fs8" [edit=sysop:move=sysop]

OCA — Wed, 20 Feb 2013 14:32:54 GMT

Protected "3fs8" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:32, 20 February 2013

OCA at 14:32, 20 February 2013

OCA — Wed, 20 Feb 2013 14:32:50 GMT

←Older revision		Revision as of 14:32, 20 February 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3fs8.png\|left\|200px]]		[[Image:3fs8.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3fs8", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3fs8\| PDB=3fs8 \| SCENE= }}		{{STRUCTURE_3fs8\| PDB=3fs8 \| SCENE= }}

	===Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA===		===Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19191736}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19191736 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19191736}}		{{ABSTRACT_PUBMED_19191736}}

	==About this Structure==		==About this Structure==
-	~~3FS8~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA].	+	[[3fs8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19191736~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019191736</ref><references group="xtra"/>
	[[Category: Thermoanaerobacterium thermosaccharolyticum]]		[[Category: Thermoanaerobacterium thermosaccharolyticum]]
	[[Category: Holden, H M.]]		[[Category: Holden, H M.]]
Line 30:		Line 18:
	[[Category: Deoxysugar]]		[[Category: Deoxysugar]]
	[[Category: Natural product]]		[[Category: Natural product]]
-		+	[[Category: Transferase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed May 6 09:26:35 2009''~~	+

OCA at 06:26, 6 May 2009

OCA — Wed, 06 May 2009 06:26:36 GMT

←Older revision		Revision as of 06:26, 6 May 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3fs8.~~jpg~~\|left\|200px]]	+	[[Image:3fs8.png\|left\|200px]]

	<!--		<!--
Line 12:		Line 12:
	===Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA===		===Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19191736}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19191736 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19191736}}

	==About this Structure==		==About this Structure==
	3FS8 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA].		3FS8 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19191736</ref><references group="xtra"/>
	[[Category: Thermoanaerobacterium thermosaccharolyticum]]		[[Category: Thermoanaerobacterium thermosaccharolyticum]]
	[[Category: Holden, H M.]]		[[Category: Holden, H M.]]
Line 22:		Line 31:
	[[Category: Natural product]]		[[Category: Natural product]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Feb 18~~ 09:26:38 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 09:26:35 2009''

OCA at 07:26, 18 February 2009

OCA — Wed, 18 Feb 2009 07:26:38 GMT

←Older revision		Revision as of 07:26, 18 February 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3fs8.jpg\|left\|200px]]

-	The ~~entry 3fs8~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3fs8", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3fs8\| PDB=3fs8 \| SCENE= }}

-	~~Authors: Holden~~, ~~H.M.~~, ~~Thoden, J.B.~~	+	===Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA===

-	Description: Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jan 21 10~~:39:39 2009''	+	==About this Structure==
		+	3FS8 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FS8 OCA].
		+	[[Category: Thermoanaerobacterium thermosaccharolyticum]]
		+	[[Category: Holden, H M.]]
		+	[[Category: Thoden, J B.]]
		+	[[Category: Acetyltransferase]]
		+	[[Category: Deoxysugar]]
		+	[[Category: Natural product]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:26:38 2009''

OCA: New page: '''Unreleased structure''' The entry 3fs8 is ON HOLD Authors: Holden, H.M., Thoden, J.B. Description: Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transfe...

OCA — Wed, 21 Jan 2009 08:39:40 GMT

New page: '''Unreleased structure''' The entry 3fs8 is ON HOLD Authors: Holden, H.M., Thoden, J.B. Description: Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transfe...

New page

'''Unreleased structure'''

The entry 3fs8 is ON HOLD

Authors: Holden, H.M., Thoden, J.B.

Description: Crystal structure of QdtC, the dTDP-3-amino-3,6-dideoxy-D-glucose N-acetyl transferase from Thermoanaerobacterium thermosaccharolyticum in complex with Acetyl-CoA

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 21 10:39:39 2009''