3g7j - Revision history

OCA at 15:37, 1 November 2023

OCA — Wed, 01 Nov 2023 15:37:09 GMT

←Older revision		Revision as of 15:37, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3g7j' size='340' side='right'caption='[[3g7j]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3g7j' size='340' side='right'caption='[[3g7j]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3g7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Anopheles_(cellia)_dirus~~ Anopheles ~~(cellia)~~ dirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3G7J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3g7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Anopheles_dirus Anopheles dirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3G7J FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GTX:S~~-~~HEXYLGLUTATHIONE'>GTX</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3f63\|3f63]], [[3g7i\|3g7i]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>GSTD4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~7168 Anopheles (Cellia) dirus])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3g7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g7j OCA], [https://pdbe.org/3g7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3g7j RCSB], [https://www.ebi.ac.uk/pdbsum/3g7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3g7j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3g7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g7j OCA], [https://pdbe.org/3g7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3g7j RCSB], [https://www.ebi.ac.uk/pdbsum/3g7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3g7j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9GN60_9DIPT Q9GN60_9DIPT]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Glutathione transferase~~]]	+	[[Category: Anopheles dirus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ketterman~~, A J~~]]	+	[[Category: Ketterman AJ]]
-	[[Category: Robinson~~, R C~~]]	+	[[Category: Robinson RC]]
-	[[Category: Wongsantichon, J]]	+	[[Category: Wongsantichon J]]
-	~~[[Category: Enzyme-inhibitor complex]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:41, 10 November 2021

OCA — Wed, 10 Nov 2021 07:41:12 GMT

←Older revision		Revision as of 07:41, 10 November 2021
Line 1:		Line 1:

	==Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus, Y119E, in Complex with S-Hexyl Glutathione==		==Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus, Y119E, in Complex with S-Hexyl Glutathione==
-	<StructureSection load='3g7j' size='340' side='right' caption='[[3g7j]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='3g7j' size='340' side='right'caption='[[3g7j]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3g7j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Anopheles_(cellia)_dirus Anopheles (cellia) dirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3G7J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3g7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Anopheles_(cellia)_dirus Anopheles (cellia) dirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3G7J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3f63\|3f63]], [[3g7i\|3g7i]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3f63\|3f63]], [[3g7i\|3g7i]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GSTD4 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7168 Anopheles (Cellia) dirus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GSTD4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7168 Anopheles (Cellia) dirus])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3g7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g7j OCA], [~~http~~://pdbe.org/3g7j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3g7j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3g7j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3g7j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3g7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g7j OCA], [https://pdbe.org/3g7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3g7j RCSB], [https://www.ebi.ac.uk/pdbsum/3g7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3g7j ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Glutathione S-transferase\|Glutathione S-transferase]]	+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
		+	[[Category: Large Structures]]
	[[Category: Ketterman, A J]]		[[Category: Ketterman, A J]]
	[[Category: Robinson, R C]]		[[Category: Robinson, R C]]

OCA at 08:05, 5 December 2018

OCA — Wed, 05 Dec 2018 08:05:03 GMT

←Older revision		Revision as of 08:05, 5 December 2018
Line 1:		Line 1:
		+
	==Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus, Y119E, in Complex with S-Hexyl Glutathione==		==Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus, Y119E, in Complex with S-Hexyl Glutathione==
	<StructureSection load='3g7j' size='340' side='right' caption='[[3g7j]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3g7j' size='340' side='right' caption='[[3g7j]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GSTD4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7168 Anopheles (Cellia) dirus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GSTD4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7168 Anopheles (Cellia) dirus])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3g7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g7j OCA], [http://pdbe.org/3g7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3g7j RCSB], [http://www.ebi.ac.uk/pdbsum/3g7j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3g7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g7j OCA], [http://pdbe.org/3g7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3g7j RCSB], [http://www.ebi.ac.uk/pdbsum/3g7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3g7j ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/3g7j_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/3g7j_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 01:39, 8 February 2016

OCA — Mon, 08 Feb 2016 01:39:35 GMT

←Older revision		Revision as of 01:39, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='3g7j' size='340' side='right' caption='[[3g7j]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3g7j' size='340' side='right' caption='[[3g7j]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3g7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Anopheles_dirus~~ Anopheles dirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3G7J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3g7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anopheles_(cellia)_dirus Anopheles (cellia) dirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3G7J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3f63\|3f63]], [[3g7i\|3g7i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3f63\|3f63]], [[3g7i\|3g7i]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GSTD4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7168 Anopheles dirus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GSTD4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7168 Anopheles (Cellia) dirus])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3g7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3g7j RCSB], [http://www.ebi.ac.uk/pdbsum/3g7j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3g7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g7j OCA], [http://pdbe.org/3g7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3g7j RCSB], [http://www.ebi.ac.uk/pdbsum/3g7j PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3g7j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3g7j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Anopheles dirus]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Ketterman, A J]]		[[Category: Ketterman, A J]]

OCA at 10:25, 3 December 2014

OCA — Wed, 03 Dec 2014 10:25:31 GMT

←Older revision		Revision as of 10:25, 3 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3g7j.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus, Y119E, in Complex with S-Hexyl Glutathione==
		+	<StructureSection load='3g7j' size='340' side='right' caption='[[3g7j]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3g7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anopheles_dirus Anopheles dirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3G7J FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3f63\|3f63]], [[3g7i\|3g7i]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GSTD4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7168 Anopheles dirus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3g7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3g7j RCSB], [http://www.ebi.ac.uk/pdbsum/3g7j PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/3g7j_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	GST (glutathione transferase) is a dimeric enzyme recognized for biotransformation of xenobiotics and endogenous toxic compounds. In the present study, residues forming the hydrophobic substrate-binding site (H-site) of a Delta class enzyme were investigated in detail for the first time by site-directed mutagenesis and crystallographic studies. Enzyme kinetics reveal that Tyr111 indirectly stabilizes GSH binding, Tyr119 modulates hydrophobic substrate binding and Phe123 indirectly modulates catalysis. Mutations at Tyr111 and Phe123 also showed evidence for positive co-operativity for GSH and 1-chloro-2,4-dinitrobenzene respectively, strongly suggesting a role for these residues in manipulating subunit-subunit communication. In the present paper we report crystal structures of the wild-type enzyme, and two mutants, in complex with S-hexylglutathione. This study has identified an aromatic 'zipper' in the H-site contributing a network of aromatic pi-pi interactions. Several residues of the cluster directly interact with the hydrophobic substrate, whereas others indirectly maintain conformational stability of the dimeric structure through the C-terminal domain (domain II). The Y119E mutant structure shows major main-chain rearrangement of domain II. This reorganization is moderated through the 'zipper' that contributes to the H-site remodelling, thus illustrating a role in co-substrate binding modulation. The F123A structure shows molecular rearrangement of the H-site in one subunit, but not the other, explaining weakened hydrophobic substrate binding and kinetic co-operativity effects of Phe123 mutations. The three crystal structures provide comprehensive evidence of the aromatic 'zipper' residues having an impact upon protein stability, catalysis and specificity. Consequently, 'zipper' residues appear to modulate and co-ordinate substrate processing through permissive flexing.

-	~~{{STRUCTURE_3g7j\| PDB=3g7j \| SCENE= }}~~	+	Structural contributions of delta class glutathione transferase active-site residues to catalysis.,Wongsantichon J, Robinson RC, Ketterman AJ Biochem J. 2010 Apr 28;428(1):25-32. PMID:20196771<ref>PMID:20196771</ref>

-	~~===Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus~~, ~~Y119E, in Complex with S-Hexyl Glutathione===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20196771}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3g7j]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Anopheles_dirus Anopheles dirus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA].~~	+

	==See Also==		==See Also==
	*[[Glutathione S-transferase\|Glutathione S-transferase]]		*[[Glutathione S-transferase\|Glutathione S-transferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020196771<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Anopheles dirus]]		[[Category: Anopheles dirus]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
-	[[Category: Ketterman, A J.]]	+	[[Category: Ketterman, A J]]
-	[[Category: Robinson, R C.]]	+	[[Category: Robinson, R C]]
-	[[Category: Wongsantichon, J.]]	+	[[Category: Wongsantichon, J]]
	[[Category: Enzyme-inhibitor complex]]		[[Category: Enzyme-inhibitor complex]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA: Protected "3g7j" [edit=sysop:move=sysop]

OCA — Wed, 20 Feb 2013 14:44:19 GMT

Protected "3g7j" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:44, 20 February 2013

OCA at 14:44, 20 February 2013

OCA — Wed, 20 Feb 2013 14:44:14 GMT

←Older revision		Revision as of 14:44, 20 February 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3g7j.png\|left\|200px]]		[[Image:3g7j.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3g7j", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3g7j\| PDB=3g7j \| SCENE= }}		{{STRUCTURE_3g7j\| PDB=3g7j \| SCENE= }}

	===Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus, Y119E, in Complex with S-Hexyl Glutathione===		===Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus, Y119E, in Complex with S-Hexyl Glutathione===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20196771}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20196771 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20196771}}		{{ABSTRACT_PUBMED_20196771}}

	==About this Structure==		==About this Structure==
-	~~3G7J~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Anopheles_dirus Anopheles dirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA].	+	[[3g7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anopheles_dirus Anopheles dirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA].
		+
		+	==See Also==
		+	*[[Glutathione S-transferase\|Glutathione S-transferase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20196771~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020196771</ref><references group="xtra"/>
	[[Category: Anopheles dirus]]		[[Category: Anopheles dirus]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
Line 31:		Line 22:
	[[Category: Enzyme-inhibitor complex]]		[[Category: Enzyme-inhibitor complex]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 12 10:29:01 2010''

OCA at 07:29, 12 May 2010

OCA — Wed, 12 May 2010 07:29:02 GMT

←Older revision		Revision as of 07:29, 12 May 2010
Line 12:		Line 12:
	===Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus, Y119E, in Complex with S-Hexyl Glutathione===		===Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus, Y119E, in Complex with S-Hexyl Glutathione===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20196771}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20196771 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20196771}}

	==About this Structure==		==About this Structure==
	3G7J is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Anopheles_dirus Anopheles dirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA].		3G7J is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Anopheles_dirus Anopheles dirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20196771</ref><references group="xtra"/>
	[[Category: Anopheles dirus]]		[[Category: Anopheles dirus]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
Line 23:		Line 32:
	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar~~ 10 15:13:14 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 12 10:29:01 2010''

OCA at 13:13, 10 March 2010

OCA — Wed, 10 Mar 2010 13:13:15 GMT

←Older revision		Revision as of 13:13, 10 March 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3g7j.~~jpg~~\|left\|200px]]	+	[[Image:3g7j.png\|left\|200px]]

	<!--		<!--
Line 23:		Line 23:
	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Feb 17~~ 10~~:44~~:13 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 10 15:13:14 2010''

OCA at 08:44, 17 February 2010

OCA — Wed, 17 Feb 2010 08:44:14 GMT

←Older revision		Revision as of 08:44, 17 February 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3g7j.jpg\|left\|200px]]

-	The ~~entry 3g7j~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3g7j", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3g7j\| PDB=3g7j \| SCENE= }}

-	~~Authors: Wongsantichon~~, J., ~~Robinson, R.C., Ketterman, A.J.~~	+	===Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus, Y119E, in Complex with S-Hexyl Glutathione===

-	Description: Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus, Y119E, in Complex with S-Hexyl Glutathione

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar 11 11:~~10:~~54 2009~~''	+	==About this Structure==
		+	3G7J is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Anopheles_dirus Anopheles dirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7J OCA].
		+	[[Category: Anopheles dirus]]
		+	[[Category: Glutathione transferase]]
		+	[[Category: Ketterman, A J.]]
		+	[[Category: Robinson, R C.]]
		+	[[Category: Wongsantichon, J.]]
		+	[[Category: Enzyme-inhibitor complex]]
		+	[[Category: Transferase]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 17 10:44:13 2010''