3gbr - Revision history

OCA at 15:38, 1 November 2023

OCA — Wed, 01 Nov 2023 15:38:20 GMT

←Older revision		Revision as of 15:38, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3gbr' size='340' side='right'caption='[[3gbr]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='3gbr' size='340' side='right'caption='[[3gbr]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gbr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_35091 Atcc 35091~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GBR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gbr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GBR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2gvq\|2gvq]], [[1o17\|1o17]], [[1gxb\|1gxb]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">trpD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Anthranilate_phosphoribosyltransferase Anthranilate phosphoribosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.18 2.4.2.18] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gbr OCA], [https://pdbe.org/3gbr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gbr RCSB], [https://www.ebi.ac.uk/pdbsum/3gbr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gbr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gbr OCA], [https://pdbe.org/3gbr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gbr RCSB], [https://www.ebi.ac.uk/pdbsum/3gbr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gbr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~TRPD_SULSO TRPD_SULSO]~~] Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).<ref>PMID:11298741</ref> <ref>PMID:16714288</ref>	+	[https://www.uniprot.org/uniprot/TRPD_SACS2 TRPD_SACS2] Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).[HAMAP-Rule:MF_00211]<ref>PMID:11298741</ref> <ref>PMID:16714288</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Anthranilate phosphoribosyltransferase]]
-	[[Category: Atcc 35091]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bruning, M]]	+	[[Category: Saccharolobus solfataricus]]
-	[[Category: Deuss, M]]	+	[[Category: Bruning M]]
-	[[Category: Ivens, A]]	+	[[Category: Deuss M]]
-	[[Category: Mayans, O]]	+	[[Category: Ivens A]]
-	[[Category: Schlee, S]]	+	[[Category: Mayans O]]
-	[[Category: Sterner, R]]	+	[[Category: Schlee S]]
-	~~[[Category: Amino-acid biosynthesis]]~~	+	[[Category: Sterner R]]
-	~~[[Category: Aromatic amino acid biosynthesis]]~~	+
-	~~[[Category: Glycosyltransferase]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Tryptophan biosynthesis~~]]	+

OCA at 07:41, 10 November 2021

OCA — Wed, 10 Nov 2021 07:41:24 GMT

←Older revision		Revision as of 07:41, 10 November 2021
Line 1:		Line 1:

	==Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus==		==Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus==
-	<StructureSection load='3gbr' size='340' side='right' caption='[[3gbr]], [[Resolution\|resolution]] 2.25Å' scene=''>	+	<StructureSection load='3gbr' size='340' side='right'caption='[[3gbr]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gbr]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3GBR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gbr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GBR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gvq\|2gvq]], [[1o17\|1o17]], [[1gxb\|1gxb]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2gvq\|2gvq]], [[1o17\|1o17]], [[1gxb\|1gxb]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">trpD ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">trpD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Anthranilate_phosphoribosyltransferase Anthranilate phosphoribosyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.18 2.4.2.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Anthranilate_phosphoribosyltransferase Anthranilate phosphoribosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.18 2.4.2.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3gbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gbr OCA], [~~http~~://pdbe.org/3gbr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3gbr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3gbr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3gbr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gbr OCA], [https://pdbe.org/3gbr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gbr RCSB], [https://www.ebi.ac.uk/pdbsum/3gbr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gbr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TRPD_SULSO TRPD_SULSO]] Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).<ref>PMID:11298741</ref> <ref>PMID:16714288</ref>	+	[[https://www.uniprot.org/uniprot/TRPD_SULSO TRPD_SULSO]] Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).<ref>PMID:11298741</ref> <ref>PMID:16714288</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gb/3gbr_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gb/3gbr_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:
	</div>		</div>
	<div class="pdbe-citations 3gbr" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3gbr" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphoribosyltransferase 3D structures\|Phosphoribosyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 40:
	[[Category: Anthranilate phosphoribosyltransferase]]		[[Category: Anthranilate phosphoribosyltransferase]]
	[[Category: Atcc 35091]]		[[Category: Atcc 35091]]
		+	[[Category: Large Structures]]
	[[Category: Bruning, M]]		[[Category: Bruning, M]]
	[[Category: Deuss, M]]		[[Category: Deuss, M]]

OCA at 07:02, 1 November 2017

OCA — Wed, 01 Nov 2017 07:02:20 GMT

←Older revision		Revision as of 07:02, 1 November 2017
Line 31:		Line 31:
	</div>		</div>
	<div class="pdbe-citations 3gbr" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3gbr" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Phosphoribosyltransferase\|Phosphoribosyltransferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:15, 5 August 2016

OCA — Fri, 05 Aug 2016 10:15:49 GMT

←Older revision		Revision as of 10:15, 5 August 2016
Line 1:		Line 1:
		+
	==Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus==		==Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus==
	<StructureSection load='3gbr' size='340' side='right' caption='[[3gbr]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='3gbr' size='340' side='right' caption='[[3gbr]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gbr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus Sulfolobus solfataricus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GBR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gbr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GBR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gvq\|2gvq]], [[1o17\|1o17]], [[1gxb\|1gxb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gvq\|2gvq]], [[1o17\|1o17]], [[1gxb\|1gxb]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">trpD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ~~Sulfolobus solfataricus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">trpD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_phosphoribosyltransferase Anthranilate phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.18 2.4.2.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_phosphoribosyltransferase Anthranilate phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.18 2.4.2.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gbr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gbr RCSB], [http://www.ebi.ac.uk/pdbsum/3gbr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gbr OCA], [http://pdbe.org/3gbr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gbr RCSB], [http://www.ebi.ac.uk/pdbsum/3gbr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gbr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gbr ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 30:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3gbr" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Anthranilate phosphoribosyltransferase]]		[[Category: Anthranilate phosphoribosyltransferase]]
-	[[Category: ~~Sulfolobus solfataricus~~]]	+	[[Category: Atcc 35091]]
	[[Category: Bruning, M]]		[[Category: Bruning, M]]
	[[Category: Deuss, M]]		[[Category: Deuss, M]]

OCA at 17:58, 25 December 2014

OCA — Thu, 25 Dec 2014 17:58:28 GMT

←Older revision		Revision as of 17:58, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gbr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gbr RCSB], [http://www.ebi.ac.uk/pdbsum/3gbr PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gbr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gbr RCSB], [http://www.ebi.ac.uk/pdbsum/3gbr PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TRPD_SULSO TRPD_SULSO]] Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).<ref>PMID:11298741</ref> <ref>PMID:16714288</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:22, 3 December 2014

OCA — Wed, 03 Dec 2014 10:22:06 GMT

←Older revision		Revision as of 10:22, 3 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3gbr.~~png~~\|~~left~~\|200px]]	+	==Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus==
		+	<StructureSection load='3gbr' size='340' side='right' caption='[[3gbr]], [[Resolution\|resolution]] 2.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3gbr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GBR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gvq\|2gvq]], [[1o17\|1o17]], [[1gxb\|1gxb]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">trpD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_phosphoribosyltransferase Anthranilate phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.18 2.4.2.18] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gbr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gbr RCSB], [http://www.ebi.ac.uk/pdbsum/3gbr PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gb/3gbr_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Anthranilate phosphoribosyltransferase from the hyperthermophilic archaeon Sulfolobus solfataricus (ssAnPRT) is encoded by the sstrpD gene and catalyzes the reaction of anthranilate (AA) with a complex of Mg(2+) and 5'-phosphoribosyl-alpha1-pyrophosphate (Mg.PRPP) to N-(5'-phosphoribosyl)-anthranilate (PRA) and pyrophosphate (PP(i)) within tryptophan biosynthesis. The ssAnPRT enzyme is highly thermostable (half-life at 85 degrees C = 35 min) but only marginally active at ambient temperatures (turnover number at 37 degrees C = 0.33 s(-1)). To understand the reason for the poor catalytic proficiency of ssAnPRT, we have isolated from an sstrpD library the activated ssAnPRT-D83G + F149S double mutant by metabolic complementation of an auxotrophic Escherichia coli strain. Whereas the activity of purified wild-type ssAnPRT is strongly reduced in the presence of high concentrations of Mg(2+) ions, this inhibition is no longer observed in the double mutant and the ssAnPRT-D83G single mutant. The comparison of the crystal structures of activated and wild-type ssAnPRT shows that the D83G mutation alters the binding mode of the substrate Mg.PRPP. Analysis of PRPP and Mg(2+)-dependent enzymatic activity indicates that this leads to a decreased affinity for a second Mg(2+) ion and thus reduces the concentration of enzymes with the inhibitory Mg(2).PRPP complex bound to the active site. Moreover, the turnover number of the double mutant ssAnPRT-D83G + F149S is elevated 40-fold compared to the wild-type enzyme, which can be attributed to an accelerated release of the product PRA. This effect appears to be mainly caused by an increased conformational flexibility induced by the F149S mutation, a hypothesis which is supported by the reduced thermal stability of the ssAnPRT-F149S single mutant.

-	~~{{STRUCTURE_3gbr\| PDB=3gbr \| SCENE= }}~~	+	Activation of anthranilate phosphoribosyltransferase from Sulfolobus solfataricus by removal of magnesium inhibition and acceleration of product release .,Schlee S, Deuss M, Bruning M, Ivens A, Schwab T, Hellmann N, Mayans O, Sterner R Biochemistry. 2009 Jun 16;48(23):5199-209. PMID:19385665<ref>PMID:19385665</ref>

-	~~===Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19385665}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3gbr]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3GBR OCA].~~	+

	==See Also==		==See Also==
	*[[Phosphoribosyltransferase\|Phosphoribosyltransferase]]		*[[Phosphoribosyltransferase\|Phosphoribosyltransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019385665<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Anthranilate phosphoribosyltransferase]]		[[Category: Anthranilate phosphoribosyltransferase]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
-	[[Category: Bruning, M.]]	+	[[Category: Bruning, M]]
-	[[Category: Deuss, M.]]	+	[[Category: Deuss, M]]
-	[[Category: Ivens, A.]]	+	[[Category: Ivens, A]]
-	[[Category: Mayans, O.]]	+	[[Category: Mayans, O]]
-	[[Category: Schlee, S.]]	+	[[Category: Schlee, S]]
-	[[Category: Sterner, R.]]	+	[[Category: Sterner, R]]
	[[Category: Amino-acid biosynthesis]]		[[Category: Amino-acid biosynthesis]]
	[[Category: Aromatic amino acid biosynthesis]]		[[Category: Aromatic amino acid biosynthesis]]

OCA: Protected "3gbr" [edit=sysop:move=sysop]

OCA — Wed, 20 Feb 2013 14:39:29 GMT

Protected "3gbr" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:39, 20 February 2013

OCA at 14:39, 20 February 2013

OCA — Wed, 20 Feb 2013 14:39:26 GMT

←Older revision		Revision as of 14:39, 20 February 2013
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3gbr.png\|left\|200px]]
-	[[Image:3gbr.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3gbr", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3gbr\| PDB=3gbr \| SCENE= }}		{{STRUCTURE_3gbr\| PDB=3gbr \| SCENE= }}

	===Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus===		===Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19385665}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19385665 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19385665}}		{{ABSTRACT_PUBMED_19385665}}

	==About this Structure==		==About this Structure==
-	~~3GBR~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBR OCA].	+	[[3gbr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBR OCA].
		+
		+	==See Also==
		+	*[[Phosphoribosyltransferase\|Phosphoribosyltransferase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19385665~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019385665</ref><references group="xtra"/>
	[[Category: Anthranilate phosphoribosyltransferase]]		[[Category: Anthranilate phosphoribosyltransferase]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
Line 37:		Line 28:
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Tryptophan biosynthesis]]		[[Category: Tryptophan biosynthesis]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 16 13:09:43 2009''

OCA at 11:09, 16 December 2009

OCA — Wed, 16 Dec 2009 11:09:45 GMT

←Older revision		Revision as of 11:09, 16 December 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3gbr.jpg\|left\|200px]]

-	The ~~entry 3gbr~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3gbr", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3gbr\| PDB=3gbr \| SCENE= }}

-	~~Authors: Bruning, M., Schlee,~~ S.~~, Deuss, M., Ivens, A., Mayans, O., Sterner, R.~~	+	===Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus===

-	Description: Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Sep 3 15~~:18:10 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19385665}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19385665 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19385665}}
		+
		+	==About this Structure==
		+	3GBR is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBR OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19385665</ref><references group="xtra"/>
		+	[[Category: Anthranilate phosphoribosyltransferase]]
		+	[[Category: Sulfolobus solfataricus]]
		+	[[Category: Bruning, M.]]
		+	[[Category: Deuss, M.]]
		+	[[Category: Ivens, A.]]
		+	[[Category: Mayans, O.]]
		+	[[Category: Schlee, S.]]
		+	[[Category: Sterner, R.]]
		+	[[Category: Amino-acid biosynthesis]]
		+	[[Category: Aromatic amino acid biosynthesis]]
		+	[[Category: Glycosyltransferase]]
		+	[[Category: Transferase]]
		+	[[Category: Tryptophan biosynthesis]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 16 13:09:43 2009''

OCA at 12:18, 3 September 2009

OCA — Thu, 03 Sep 2009 12:18:10 GMT

←Older revision		Revision as of 12:18, 3 September 2009
Line 3:		Line 3:
	The entry 3gbr is ON HOLD until Paper Publication		The entry 3gbr is ON HOLD until Paper Publication

-	Authors: Schlee, S., Deuss~~, M., Bruning~~, M., Ivens, A~~., Schwab, T., Hellmann, N~~., Mayans, O., Sterner, R.	+	Authors: Bruning, M., Schlee, S., Deuss, M., Ivens, A., Mayans, O., Sterner, R.

	Description: Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus		Description: Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Mar 25 12~~:14:07 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 15:18:10 2009''