3gqj - Revision history

OCA at 09:56, 21 February 2024

OCA — Wed, 21 Feb 2024 09:56:03 GMT

←Older revision		Revision as of 09:56, 21 February 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CIF_PHOLL CIF_PHOLL] Protein-glutamine deamidase effector that inhibits the host cell cycle and other key cellular processes such as the actin network and programmed-cell death (PubMed:19308257, PubMed:19225106, PubMed:20870031). Acts by mediating the side chain deamidation of 'Gln-40' of host NEDD8, converting it to glutamate, thereby abolishing the activity of cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes) (By similarity). Inactivation of CRL complexes prevents ubiquitination and subsequent degradation of the cyclin-dependent kinase inhibitors CDKN1A/p21 and CDKN1B/p27, leading to G1 and G2 cell cycle arrests in host cells (PubMed:19308257). Deamidation of 'Gln-40' of host NEDD8 also triggers macrophage-specific programmed cell death (By similarity). Also able to catalyze deamidation of 'Gln-40' of host ubiquitin in vitro; however, NEDD8 constitutes the preferred substrate in vivo (By similarity).[UniProtKB:Q63KH5]<ref>PMID:19225106</ref> <ref>PMID:19308257</ref> <ref>PMID:20870031</ref>		[https://www.uniprot.org/uniprot/CIF_PHOLL CIF_PHOLL] Protein-glutamine deamidase effector that inhibits the host cell cycle and other key cellular processes such as the actin network and programmed-cell death (PubMed:19308257, PubMed:19225106, PubMed:20870031). Acts by mediating the side chain deamidation of 'Gln-40' of host NEDD8, converting it to glutamate, thereby abolishing the activity of cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes) (By similarity). Inactivation of CRL complexes prevents ubiquitination and subsequent degradation of the cyclin-dependent kinase inhibitors CDKN1A/p21 and CDKN1B/p27, leading to G1 and G2 cell cycle arrests in host cells (PubMed:19308257). Deamidation of 'Gln-40' of host NEDD8 also triggers macrophage-specific programmed cell death (By similarity). Also able to catalyze deamidation of 'Gln-40' of host ubiquitin in vitro; however, NEDD8 constitutes the preferred substrate in vivo (By similarity).[UniProtKB:Q63KH5]<ref>PMID:19225106</ref> <ref>PMID:19308257</ref> <ref>PMID:20870031</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	A pre-requisite for bacterial pathogenesis is the successful interaction of a pathogen with a host. One mechanism used by a broad range of Gram negative bacterial pathogens is to deliver effector proteins directly into host cells through a dedicated type III secretion system where they modulate host cell function. The cycle inhibiting factor (Cif) family of effector proteins, identified in a growing number of pathogens that harbour functional type III secretion systems and have a wide host range, arrest the eukaryotic cell cycle. Here, the crystal structures of Cifs from the insect pathogen/nematode symbiont Photorhabdus luminescens (a gamma-proteobacterium) and human pathogen Burkholderia pseudomallei (a beta-proteobacterium) are presented. Both of these proteins adopt an overall fold similar to the papain sub-family of cysteine proteases, as originally identified in the structure of a truncated form of Cif from Enteropathogenic E. coli (EPEC), despite sharing only limited sequence identity. The structure of an N-terminal region, referred to here as the 'tail-domain' (absent in the EPEC Cif structure), suggests a surface likely to be involved in host-cell substrate recognition. The conformation of the Cys-His-Gln catalytic triad is retained, and the essential cysteine is exposed to solvent and addressable by small molecule reagents. These structures and biochemical work contribute to the rapidly expanding literature on Cifs, and direct further studies to better understand the molecular details of the activity of these proteins.
-
-	Crystal structures of Cif from bacterial pathogens Photorhabdus luminescens and Burkholderia pseudomallei.,Crow A, Race PR, Jubelin G, Varela Chavez C, Escoubas JM, Oswald E, Banfield MJ PLoS ONE. 2009;4(5):e5582. Epub 2009 May 18. PMID:19440549<ref>PMID:19440549</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3gqj" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:55, 20 December 2023

OCA — Wed, 20 Dec 2023 12:55:03 GMT

←Older revision		Revision as of 12:55, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='3gqj' size='340' side='right'caption='[[3gqj]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3gqj' size='340' side='right'caption='[[3gqj]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gqj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ccm_7076 Ccm 7076~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GQJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GQJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gqj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_laumondii_subsp._laumondii Photorhabdus laumondii subsp. laumondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GQJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GQJ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3gqm\|3gqm]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">plu2515 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=141679 CCM 7076])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gqj OCA], [https://pdbe.org/3gqj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gqj RCSB], [https://www.ebi.ac.uk/pdbsum/3gqj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gqj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gqj OCA], [https://pdbe.org/3gqj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gqj RCSB], [https://www.ebi.ac.uk/pdbsum/3gqj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gqj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CIF_PHOLL CIF_PHOLL] Protein-glutamine deamidase effector that inhibits the host cell cycle and other key cellular processes such as the actin network and programmed-cell death (PubMed:19308257, PubMed:19225106, PubMed:20870031). Acts by mediating the side chain deamidation of 'Gln-40' of host NEDD8, converting it to glutamate, thereby abolishing the activity of cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes) (By similarity). Inactivation of CRL complexes prevents ubiquitination and subsequent degradation of the cyclin-dependent kinase inhibitors CDKN1A/p21 and CDKN1B/p27, leading to G1 and G2 cell cycle arrests in host cells (PubMed:19308257). Deamidation of 'Gln-40' of host NEDD8 also triggers macrophage-specific programmed cell death (By similarity). Also able to catalyze deamidation of 'Gln-40' of host ubiquitin in vitro; however, NEDD8 constitutes the preferred substrate in vivo (By similarity).[UniProtKB:Q63KH5]<ref>PMID:19225106</ref> <ref>PMID:19308257</ref> <ref>PMID:20870031</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Ccm 7076]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Banfield, M J~~]]	+	[[Category: Photorhabdus laumondii subsp. laumondii]]
-	[[Category: ~~Crow, A~~]]	+	[[Category: Banfield MJ]]
-	[[Category: ~~Cell inhibiting factor]]~~	+	[[Category: Crow A]]
-	~~[[Category: Cif]]~~	+
-	~~[[Category: Protease-like]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 07:52, 16 March 2022

OCA — Wed, 16 Mar 2022 07:52:16 GMT

←Older revision		Revision as of 07:52, 16 March 2022
Line 1:		Line 1:

	==Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens==		==Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens==
-	<StructureSection load='3gqj' size='340' side='right' caption='[[3gqj]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='3gqj' size='340' side='right'caption='[[3gqj]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gqj]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ccm_7076 Ccm 7076]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GQJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3GQJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gqj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ccm_7076 Ccm 7076]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GQJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GQJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gqm\|3gqm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3gqm\|3gqm]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plu2515 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=141679 CCM 7076])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plu2515 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=141679 CCM 7076])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3gqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gqj OCA], [~~http~~://pdbe.org/3gqj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3gqj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3gqj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3gqj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gqj OCA], [https://pdbe.org/3gqj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gqj RCSB], [https://www.ebi.ac.uk/pdbsum/3gqj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gqj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Ccm 7076]]		[[Category: Ccm 7076]]
		+	[[Category: Large Structures]]
	[[Category: Banfield, M J]]		[[Category: Banfield, M J]]
	[[Category: Crow, A]]		[[Category: Crow, A]]

OCA at 13:26, 5 August 2016

OCA — Fri, 05 Aug 2016 13:26:12 GMT

←Older revision		Revision as of 13:26, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens==		==Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens==
	<StructureSection load='3gqj' size='340' side='right' caption='[[3gqj]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3gqj' size='340' side='right' caption='[[3gqj]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gqj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Photorhabdus_luminescens_subsp._laumondii Photorhabdus luminescens subsp. laumondii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GQJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gqj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ccm_7076 Ccm 7076]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GQJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gqm\|3gqm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gqm\|3gqm]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plu2515 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=141679 ~~Photorhabdus luminescens subsp. laumondii~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plu2515 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=141679 CCM 7076])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gqj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gqj RCSB], [http://www.ebi.ac.uk/pdbsum/3gqj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gqj OCA], [http://pdbe.org/3gqj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gqj RCSB], [http://www.ebi.ac.uk/pdbsum/3gqj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gqj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3gqj" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Photorhabdus luminescens subsp. laumondii~~]]	+	[[Category: Ccm 7076]]
	[[Category: Banfield, M J]]		[[Category: Banfield, M J]]
	[[Category: Crow, A]]		[[Category: Crow, A]]

OCA at 09:05, 8 December 2014

OCA — Mon, 08 Dec 2014 09:05:15 GMT

←Older revision		Revision as of 09:05, 8 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3gqj.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens==
		+	<StructureSection load='3gqj' size='340' side='right' caption='[[3gqj]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3gqj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Photorhabdus_luminescens_subsp._laumondii Photorhabdus luminescens subsp. laumondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GQJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gqm\|3gqm]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plu2515 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=141679 Photorhabdus luminescens subsp. laumondii])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gqj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gqj RCSB], [http://www.ebi.ac.uk/pdbsum/3gqj PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A pre-requisite for bacterial pathogenesis is the successful interaction of a pathogen with a host. One mechanism used by a broad range of Gram negative bacterial pathogens is to deliver effector proteins directly into host cells through a dedicated type III secretion system where they modulate host cell function. The cycle inhibiting factor (Cif) family of effector proteins, identified in a growing number of pathogens that harbour functional type III secretion systems and have a wide host range, arrest the eukaryotic cell cycle. Here, the crystal structures of Cifs from the insect pathogen/nematode symbiont Photorhabdus luminescens (a gamma-proteobacterium) and human pathogen Burkholderia pseudomallei (a beta-proteobacterium) are presented. Both of these proteins adopt an overall fold similar to the papain sub-family of cysteine proteases, as originally identified in the structure of a truncated form of Cif from Enteropathogenic E. coli (EPEC), despite sharing only limited sequence identity. The structure of an N-terminal region, referred to here as the 'tail-domain' (absent in the EPEC Cif structure), suggests a surface likely to be involved in host-cell substrate recognition. The conformation of the Cys-His-Gln catalytic triad is retained, and the essential cysteine is exposed to solvent and addressable by small molecule reagents. These structures and biochemical work contribute to the rapidly expanding literature on Cifs, and direct further studies to better understand the molecular details of the activity of these proteins.

-	~~{{STRUCTURE_3gqj\| PDB=3gqj \| SCENE= }}~~	+	Crystal structures of Cif from bacterial pathogens Photorhabdus luminescens and Burkholderia pseudomallei.,Crow A, Race PR, Jubelin G, Varela Chavez C, Escoubas JM, Oswald E, Banfield MJ PLoS ONE. 2009;4(5):e5582. Epub 2009 May 18. PMID:19440549<ref>PMID:19440549</ref>

-	~~===Crystal structure~~ of ~~Cell Inhibiting Factor (Cif) from Photorhabdus luminescens===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19440549}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3gqj]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Photorhabdus_luminescens_subsp~~.~~_laumondii Photorhabdus luminescens subsp. laumondii]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3GQJ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019440549<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Photorhabdus luminescens subsp. laumondii]]		[[Category: Photorhabdus luminescens subsp. laumondii]]
-	[[Category: Banfield, M J.]]	+	[[Category: Banfield, M J]]
-	[[Category: Crow, A.]]	+	[[Category: Crow, A]]
	[[Category: Cell inhibiting factor]]		[[Category: Cell inhibiting factor]]
	[[Category: Cif]]		[[Category: Cif]]
	[[Category: Protease-like]]		[[Category: Protease-like]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA: Protected "3gqj" [edit=sysop:move=sysop]

OCA — Wed, 27 Feb 2013 15:39:03 GMT

Protected "3gqj" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:39, 27 February 2013

OCA at 15:39, 27 February 2013

OCA — Wed, 27 Feb 2013 15:39:00 GMT

←Older revision		Revision as of 15:39, 27 February 2013
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3gqj.png\|left\|200px]]
-	[[Image:3gqj.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3gqj", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3gqj\| PDB=3gqj \| SCENE= }}		{{STRUCTURE_3gqj\| PDB=3gqj \| SCENE= }}

	===Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens===		===Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19440549}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19440549 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_19440549}}		{{ABSTRACT_PUBMED_19440549}}

	==About this Structure==		==About this Structure==
-	~~3GQJ~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Photorhabdus_luminescens_subsp._laumondii Photorhabdus luminescens subsp. laumondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GQJ OCA].	+	[[3gqj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Photorhabdus_luminescens_subsp._laumondii Photorhabdus luminescens subsp. laumondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GQJ OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19440549~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019440549</ref><references group="xtra"/>
	[[Category: Photorhabdus luminescens subsp. laumondii]]		[[Category: Photorhabdus luminescens subsp. laumondii]]
	[[Category: Banfield, M J.]]		[[Category: Banfield, M J.]]
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	[[Category: Protease-like]]		[[Category: Protease-like]]
	[[Category: Unknown function]]		[[Category: Unknown function]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 4 07:25:44 2009''

OCA at 04:25, 4 June 2009

OCA — Thu, 04 Jun 2009 04:25:46 GMT

←Older revision		Revision as of 04:25, 4 June 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3gqj.jpg\|left\|200px]]

-	The ~~entry 3gqj~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3gqj", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3gqj\| PDB=3gqj \| SCENE= }}

-	~~Authors: Crow, A., Banfield, M.J.~~	+	===Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens===

-	Description: Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu ~~Apr 30 08~~:44:53 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19440549}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19440549 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19440549}}
		+
		+	==About this Structure==
		+	3GQJ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Photorhabdus_luminescens_subsp._laumondii Photorhabdus luminescens subsp. laumondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GQJ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19440549</ref><references group="xtra"/>
		+	[[Category: Photorhabdus luminescens subsp. laumondii]]
		+	[[Category: Banfield, M J.]]
		+	[[Category: Crow, A.]]
		+	[[Category: Cell inhibiting factor]]
		+	[[Category: Cif]]
		+	[[Category: Protease-like]]
		+	[[Category: Unknown function]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 4 07:25:44 2009''

OCA at 05:44, 30 April 2009

OCA — Thu, 30 Apr 2009 05:44:53 GMT

←Older revision		Revision as of 05:44, 30 April 2009
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3gqj is ON HOLD	+	The entry 3gqj is ON HOLD until Paper Publication

	Authors: Crow, A., Banfield, M.J.		Authors: Crow, A., Banfield, M.J.
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	Description: Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens		Description: Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ Apr ~~15 09~~:56:21 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 30 08:44:53 2009''

OCA: New page: '''Unreleased structure''' The entry 3gqj is ON HOLD Authors: Crow, A., Banfield, M.J. Description: Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens ''P...

OCA — Wed, 15 Apr 2009 06:56:21 GMT

New page: '''Unreleased structure''' The entry 3gqj is ON HOLD Authors: Crow, A., Banfield, M.J. Description: Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens ''P...

New page

'''Unreleased structure'''

The entry 3gqj is ON HOLD

Authors: Crow, A., Banfield, M.J.

Description: Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 15 09:56:21 2009''