3gu9 - Revision history

OCA at 11:51, 13 October 2021

OCA — Wed, 13 Oct 2021 11:51:43 GMT

←Older revision		Revision as of 11:51, 13 October 2021
Line 1:		Line 1:

	==R228A mutation in organophosphorus hydrolase from Deinococcus radiodurans==		==R228A mutation in organophosphorus hydrolase from Deinococcus radiodurans==
-	<StructureSection load='3gu9' size='340' side='right' caption='[[3gu9]], [[Resolution\|resolution]] 2.06Å' scene=''>	+	<StructureSection load='3gu9' size='340' side='right'caption='[[3gu9]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gu9]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3GU9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gu9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GU9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gtf\|3gtf]], [[3gth\|3gth]], [[3gti\|3gti]], [[3gtx\|3gtx]], [[3gu1\|3gu1]], [[3gu2\|3gu2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3gtf\|3gtf]], [[3gth\|3gth]], [[3gti\|3gti]], [[3gtx\|3gtx]], [[3gu1\|3gu1]], [[3gu2\|3gu2]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DR_0930 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 "Micrococcus radiodurans" Raj et al. 1960])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DR_0930 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 "Micrococcus radiodurans" Raj et al. 1960])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3gu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gu9 OCA], [~~http~~://pdbe.org/3gu9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3gu9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3gu9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3gu9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gu9 OCA], [https://pdbe.org/3gu9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gu9 RCSB], [https://www.ebi.ac.uk/pdbsum/3gu9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gu9 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Phosphotriesterase\|Phosphotriesterase]]	+	*[[Phosphotriesterase 3D structures\|Phosphotriesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Micrococcus radiodurans raj et al. 1960]]		[[Category: Micrococcus radiodurans raj et al. 1960]]
		+	[[Category: Large Structures]]
	[[Category: Hawwa, R]]		[[Category: Hawwa, R]]
	[[Category: Larsen, S]]		[[Category: Larsen, S]]

OCA at 06:01, 7 December 2018

OCA — Fri, 07 Dec 2018 06:01:20 GMT

←Older revision		Revision as of 06:01, 7 December 2018
Line 1:		Line 1:
		+
	==R228A mutation in organophosphorus hydrolase from Deinococcus radiodurans==		==R228A mutation in organophosphorus hydrolase from Deinococcus radiodurans==
	<StructureSection load='3gu9' size='340' side='right' caption='[[3gu9]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='3gu9' size='340' side='right' caption='[[3gu9]], [[Resolution\|resolution]] 2.06Å' scene=''>
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gtf\|3gtf]], [[3gth\|3gth]], [[3gti\|3gti]], [[3gtx\|3gtx]], [[3gu1\|3gu1]], [[3gu2\|3gu2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gtf\|3gtf]], [[3gth\|3gth]], [[3gti\|3gti]], [[3gtx\|3gtx]], [[3gu1\|3gu1]], [[3gu2\|3gu2]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DR_0930 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 "Micrococcus radiodurans" Raj et al. 1960])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DR_0930 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 "Micrococcus radiodurans" Raj et al. 1960])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gu9 OCA], [http://pdbe.org/3gu9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gu9 RCSB], [http://www.ebi.ac.uk/pdbsum/3gu9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gu9 OCA], [http://pdbe.org/3gu9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gu9 RCSB], [http://www.ebi.ac.uk/pdbsum/3gu9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gu9 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/3gu9_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/3gu9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 07:49, 7 February 2016

OCA — Sun, 07 Feb 2016 07:49:35 GMT

←Older revision		Revision as of 07:49, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='3gu9' size='340' side='right' caption='[[3gu9]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='3gu9' size='340' side='right' caption='[[3gu9]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gu9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Deinococcus_radiodurans Deinococcus~~ radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GU9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gu9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GU9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gtf\|3gtf]], [[3gth\|3gth]], [[3gti\|3gti]], [[3gtx\|3gtx]], [[3gu1\|3gu1]], [[3gu2\|3gu2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gtf\|3gtf]], [[3gth\|3gth]], [[3gti\|3gti]], [[3gtx\|3gtx]], [[3gu1\|3gu1]], [[3gu2\|3gu2]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DR_0930 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 ~~Deinococcus~~ radiodurans])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DR_0930 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 "Micrococcus radiodurans" Raj et al. 1960])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gu9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gu9 RCSB], [http://www.ebi.ac.uk/pdbsum/3gu9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gu9 OCA], [http://pdbe.org/3gu9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gu9 RCSB], [http://www.ebi.ac.uk/pdbsum/3gu9 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gu9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3gu9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Deinococcus~~ radiodurans]]	+	[[Category: Micrococcus radiodurans raj et al. 1960]]
	[[Category: Hawwa, R]]		[[Category: Hawwa, R]]
	[[Category: Larsen, S]]		[[Category: Larsen, S]]

OCA at 10:53, 20 January 2015

OCA — Tue, 20 Jan 2015 10:53:15 GMT

←Older revision		Revision as of 10:53, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3gu9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GU9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3gu9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GU9 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gtf\|3gtf]], [[3gth\|3gth]], [[3gti\|3gti]], [[3gtx\|3gtx]], [[3gu1\|3gu1]], [[3gu2\|3gu2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gtf\|3gtf]], [[3gth\|3gth]], [[3gti\|3gti]], [[3gtx\|3gtx]], [[3gu1\|3gu1]], [[3gu2\|3gu2]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DR_0930 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 Deinococcus radiodurans])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DR_0930 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 Deinococcus radiodurans])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gu9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gu9 RCSB], [http://www.ebi.ac.uk/pdbsum/3gu9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gu9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gu9 RCSB], [http://www.ebi.ac.uk/pdbsum/3gu9 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Deinococcus radiodurans]]		[[Category: Deinococcus radiodurans]]
-	[[Category: Hawwa, R.]]	+	[[Category: Hawwa, R]]
-	[[Category: Larsen, S.]]	+	[[Category: Larsen, S]]
-	[[Category: Mesecar, A.]]	+	[[Category: Mesecar, A]]
-	[[Category: Ratia, K.]]	+	[[Category: Ratia, K]]
	[[Category: Alpha-beta barrel]]		[[Category: Alpha-beta barrel]]
	[[Category: Amidohydrolase]]		[[Category: Amidohydrolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Mutant]]		[[Category: Mutant]]

OCA at 12:39, 29 September 2014

OCA — Mon, 29 Sep 2014 12:39:31 GMT

←Older revision		Revision as of 12:39, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3gu9.~~png~~\|~~left~~\|200px]]	+	==R228A mutation in organophosphorus hydrolase from Deinococcus radiodurans==
		+	<StructureSection load='3gu9' size='340' side='right' caption='[[3gu9]], [[Resolution\|resolution]] 2.06Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3gu9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GU9 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gtf\|3gtf]], [[3gth\|3gth]], [[3gti\|3gti]], [[3gtx\|3gtx]], [[3gu1\|3gu1]], [[3gu2\|3gu2]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DR_0930 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 Deinococcus radiodurans])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gu9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gu9 RCSB], [http://www.ebi.ac.uk/pdbsum/3gu9 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/3gu9_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	An enzyme from the amidohydrolase family from Deinococcus radiodurans (Dr-OPH) with homology to phosphotriesterase has been shown to exhibit activity against both organophosphate (OP) and lactone compounds. We have characterized the physical properties of Dr-OPH and have found it to be a highly thermostable enzyme, remaining active after 3 h of incubation at 60 degrees C and withstanding incubation at temperatures up to 70 degrees C. In addition, it can withstand concentrations of at least 200 mg/mL. These properties make Dr-OPH a promising candidate for development in commercial applications. However, compared to the most widely studied OP-degrading enzyme, that from Pseudomonas diminuta, Dr-OPH has low hydrolytic activity against certain OP substrates. Therefore, we sought to improve the OP-degrading activity of Dr-OPH, specifically toward the pesticides ethyl and methyl paraoxon, using structure-based and random approaches. Site-directed mutagenesis, random mutagenesis, and site-saturation mutagenesis were utilized to increase the OP-degrading activity of Dr-OPH. Out of a screen of more than 30,000 potential mutants, a total of 26 mutant enzymes were purified and characterized kinetically. Crystal structures of w.t. Dr-OPH, of Dr-OPH in complex with a product analog, and of 7 mutant enzymes were determined to resolutions between 1.7 and 2.4 A. Information from these structures directed the design and production of 4 additional mutants for analysis. In total, our mutagenesis efforts improved the catalytic activity of Dr-OPH toward ethyl and methyl paraoxon by 126- and 322-fold and raised the specificity for these two substrates by 557- and 183-fold, respectively. Our work highlights the importance of an iterative approach to mutagenesis, proving that large rate enhancements are achieved when mutations are made in already active mutants. In addition, the relationship between the kinetic parameters and the introduced mutations has allowed us to hypothesize on those factors most important for maintaining the structure and function of the enzyme.

-	~~{{STRUCTURE_3gu9\| PDB=3gu9 \| SCENE= }}~~	+	Structure-based and random mutagenesis approaches increase the organophosphate-degrading activity of a phosphotriesterase homologue from Deinococcus radiodurans.,Hawwa R, Larsen SD, Ratia K, Mesecar AD J Mol Biol. 2009 Oct 16;393(1):36-57. Epub 2009 Jul 22. PMID:19631223<ref>PMID:19631223</ref>

-	~~===R228A mutation in organophosphorus hydrolase from Deinococcus radiodurans===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19631223}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3gu9]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA].~~	+

	==See Also==		==See Also==
	*[[Phosphotriesterase\|Phosphotriesterase]]		*[[Phosphotriesterase\|Phosphotriesterase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019631223<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Deinococcus radiodurans]]		[[Category: Deinococcus radiodurans]]
	[[Category: Hawwa, R.]]		[[Category: Hawwa, R.]]

OCA at 06:02, 20 October 2012

OCA — Sat, 20 Oct 2012 06:02:00 GMT

←Older revision		Revision as of 06:02, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3gu9]] is a 1 chain structure ~~of [[Phosphotriesterase]]~~ with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA].	+	[[3gu9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA].

	==See Also==		==See Also==

OCA at 11:53, 25 July 2012

OCA — Wed, 25 Jul 2012 11:53:37 GMT

←Older revision		Revision as of 11:53, 25 July 2012
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	[[Image:3gu9.png\|left\|200px]]		[[Image:3gu9.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3gu9", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3gu9\| PDB=3gu9 \| SCENE= }}		{{STRUCTURE_3gu9\| PDB=3gu9 \| SCENE= }}

	===R228A mutation in organophosphorus hydrolase from Deinococcus radiodurans===		===R228A mutation in organophosphorus hydrolase from Deinococcus radiodurans===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19631223}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19631223 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19631223}}		{{ABSTRACT_PUBMED_19631223}}

	==About this Structure==		==About this Structure==
-	[[3gu9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA].	+	[[3gu9]] is a 1 chain structure of [[Phosphotriesterase]] with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA].
		+
		+	==See Also==
		+	*[[Phosphotriesterase\|Phosphotriesterase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19631223~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019631223</ref><references group="xtra"/>
	[[Category: Deinococcus radiodurans]]		[[Category: Deinococcus radiodurans]]
	[[Category: Hawwa, R.]]		[[Category: Hawwa, R.]]

OCA: Protected "3gu9" [edit=sysop:move=sysop]

OCA — Wed, 29 Dec 2010 18:30:01 GMT

Protected "3gu9" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:30, 29 December 2010

OCA at 18:30, 29 December 2010

OCA — Wed, 29 Dec 2010 18:30:00 GMT

←Older revision		Revision as of 18:30, 29 December 2010
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-	{{Seed}}
	[[Image:3gu9.png\|left\|200px]]		[[Image:3gu9.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~3GU9~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA].	+	[[3gu9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA].

	==Reference==		==Reference==
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	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Mutant]]		[[Category: Mutant]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 10 10:49:19 2010''

OCA at 06:44, 10 June 2010

OCA — Thu, 10 Jun 2010 06:44:32 GMT

←Older revision		Revision as of 06:44, 10 June 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3gu9.~~jpg~~\|left\|200px]]	+	[[Image:3gu9.png\|left\|200px]]

	<!--		<!--
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	===R228A mutation in organophosphorus hydrolase from Deinococcus radiodurans===		===R228A mutation in organophosphorus hydrolase from Deinococcus radiodurans===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19631223}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19631223 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19631223}}

	==About this Structure==		==About this Structure==
-	3GU9 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA].	+	3GU9 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GU9 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19631223</ref><references group="xtra"/>
	[[Category: Deinococcus radiodurans]]		[[Category: Deinococcus radiodurans]]
	[[Category: Hawwa, R.]]		[[Category: Hawwa, R.]]
Line 22:		Line 31:
	[[Category: Alpha-beta barrel]]		[[Category: Alpha-beta barrel]]
	[[Category: Amidohydrolase]]		[[Category: Amidohydrolase]]
		+	[[Category: Hydrolase]]
	[[Category: Mutant]]		[[Category: Mutant]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 1 09~~:40:~~21 2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 10 10:49:19 2010''