3guz - Revision history

OCA at 19:20, 29 May 2024

OCA — Wed, 29 May 2024 19:20:57 GMT

←Older revision		Revision as of 19:20, 29 May 2024
Line 3:		Line 3:
	<StructureSection load='3guz' size='340' side='right'caption='[[3guz]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='3guz' size='340' side='right'caption='[[3guz]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3guz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3guz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GUZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PAF:PANTOATE'>PAF</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.67Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>panC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~83333 ECOLI])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Pantoate--beta-alanine_ligase Pantoate--beta-alanine ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.1 6.3.2.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [https://pdbe.org/3guz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [https://www.ebi.ac.uk/pdbsum/3guz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3guz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [https://pdbe.org/3guz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [https://www.ebi.ac.uk/pdbsum/3guz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3guz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PANC_ECOLI PANC_ECOLI]] Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.<ref>PMID:357689</ref>	+	[https://www.uniprot.org/uniprot/PANC_ECOLI PANC_ECOLI] Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.<ref>PMID:357689</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Pantoate--beta-alanine ligase]]~~	+	[[Category: Chakrabarti KS]]
-	[[Category: Chakrabarti~~, K S~~]]	+	[[Category: Gopal B]]
-	[[Category: Gopal, B]]	+	[[Category: Sarma SP]]
-	[[Category: Sarma~~, S P~~]]	+	[[Category: Thakur KG]]
-	[[Category: Thakur~~, K G]]~~	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Competitive inhibition]]~~	+
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Non-canonical pantoate binding-site]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Pantothenate biosynthesis]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Substrate binding~~]]	+

OCA at 07:42, 10 November 2021

OCA — Wed, 10 Nov 2021 07:42:33 GMT

←Older revision		Revision as of 07:42, 10 November 2021
Line 1:		Line 1:

	==Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's==		==Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's==
-	<StructureSection load='3guz' size='340' side='right' caption='[[3guz]], [[Resolution\|resolution]] 1.67Å' scene=''>	+	<StructureSection load='3guz' size='340' side='right'caption='[[3guz]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3guz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3GUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3guz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GUZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Pantoate--beta-alanine_ligase Pantoate--beta-alanine ligase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.1 6.3.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Pantoate--beta-alanine_ligase Pantoate--beta-alanine ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.1 6.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [~~http~~://pdbe.org/3guz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3guz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3guz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [https://pdbe.org/3guz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [https://www.ebi.ac.uk/pdbsum/3guz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3guz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PANC_ECOLI PANC_ECOLI]] Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.<ref>PMID:357689</ref>	+	[[https://www.uniprot.org/uniprot/PANC_ECOLI PANC_ECOLI]] Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.<ref>PMID:357689</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Pantoate--beta-alanine ligase]]		[[Category: Pantoate--beta-alanine ligase]]
	[[Category: Chakrabarti, K S]]		[[Category: Chakrabarti, K S]]

OCA at 05:32, 7 December 2018

OCA — Fri, 07 Dec 2018 05:32:22 GMT

←Older revision		Revision as of 05:32, 7 December 2018
Line 1:		Line 1:
		+
	==Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's==		==Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's==
	<StructureSection load='3guz' size='340' side='right' caption='[[3guz]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='3guz' size='340' side='right' caption='[[3guz]], [[Resolution\|resolution]] 1.67Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantoate--beta-alanine_ligase Pantoate--beta-alanine ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.1 6.3.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantoate--beta-alanine_ligase Pantoate--beta-alanine ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.1 6.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [http://pdbe.org/3guz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [http://www.ebi.ac.uk/pdbsum/3guz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [http://pdbe.org/3guz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [http://www.ebi.ac.uk/pdbsum/3guz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3guz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/3guz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/3guz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 44:		Line 45:
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Competitive inhibition]]		[[Category: Competitive inhibition]]
		+	[[Category: Cytoplasm]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Non-canonical pantoate binding-site]]		[[Category: Non-canonical pantoate binding-site]]

OCA at 13:09, 9 February 2016

OCA — Tue, 09 Feb 2016 13:09:03 GMT

←Older revision		Revision as of 13:09, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3guz' size='340' side='right' caption='[[3guz]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='3guz' size='340' side='right' caption='[[3guz]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3guz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3guz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GUZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantoate--beta-alanine_ligase Pantoate--beta-alanine ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.1 6.3.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantoate--beta-alanine_ligase Pantoate--beta-alanine ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.1 6.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [http://www.ebi.ac.uk/pdbsum/3guz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [http://pdbe.org/3guz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [http://www.ebi.ac.uk/pdbsum/3guz PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3guz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3guz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Ecoli]]
	[[Category: Pantoate--beta-alanine ligase]]		[[Category: Pantoate--beta-alanine ligase]]
	[[Category: Chakrabarti, K S]]		[[Category: Chakrabarti, K S]]

OCA at 07:01, 25 December 2014

OCA — Thu, 25 Dec 2014 07:01:13 GMT

←Older revision		Revision as of 07:01, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [http://www.ebi.ac.uk/pdbsum/3guz PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [http://www.ebi.ac.uk/pdbsum/3guz PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PANC_ECOLI PANC_ECOLI]] Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.<ref>PMID:357689</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:06, 8 December 2014

OCA — Mon, 08 Dec 2014 09:06:01 GMT

←Older revision		Revision as of 09:06, 8 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3guz.~~png~~\|~~left~~\|200px]]	+	==Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's==
		+	<StructureSection load='3guz' size='340' side='right' caption='[[3guz]], [[Resolution\|resolution]] 1.67Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3guz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GUZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantoate--beta-alanine_ligase Pantoate--beta-alanine ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.1 6.3.2.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [http://www.ebi.ac.uk/pdbsum/3guz PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/3guz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The structural basis for the homotropic inhibition of pantothenate synthetase by the substrate pantoate was investigated by X-ray crystallography and high-resolution NMR spectroscopic methods. The tertiary structure of the dimeric N-terminal domain of Escherichia coli pantothenate synthetase, determined by X-ray crystallography to a resolution of 1.7 A, showed a second molecule of pantoate bound in the ATP-binding pocket. Pantoate binding to the ATP-binding site induced large changes in structure, mainly for backbone and side chain atoms of residues in the ATP binding HXGH(34-37) motif. Sequence-specific NMR resonance assignments and solution secondary structure of the dimeric N-terminal domain, obtained using samples enriched in (2)H, (13)C, and (15)N, indicated that the secondary structural elements were conserved in solution. Nitrogen-15 edited two-dimensional solution NMR chemical shift mapping experiments revealed that pantoate, at 10 mm, bound at these two independent sites. The solution NMR studies unambiguously demonstrated that ATP stoichiometrically displaced pantoate from the ATP-binding site. All NMR and X-ray studies were conducted at substrate concentrations used for enzymatic characterization of pantothenate synthetase from different sources [Jonczyk R & Genschel U (2006) J Biol Chem 281, 37435-37446]. As pantoate binding to its canonical site is structurally conserved, these results demonstrate that the observed homotropic effects of pantoate on pantothenate biosynthesis are caused by competitive binding of this substrate to the ATP-binding site. The results presented here have implications for the design and development of potential antibacterial and herbicidal agents.

-	~~{{STRUCTURE_3guz\| PDB=3guz \| SCENE= }}~~	+	X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate.,Chakrabarti KS, Thakur KG, Gopal B, Sarma SP FEBS J. 2010 Feb;277(3):697-712. Epub 2010 Jan 4. PMID:20059543<ref>PMID:20059543</ref>

-	~~===Structural and substrate-binding studies~~ of ~~pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20059543}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3guz]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA].~~	+

	==See Also==		==See Also==
	*[[Pantothenate synthetase\|Pantothenate synthetase]]		*[[Pantothenate synthetase\|Pantothenate synthetase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020059543<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Pantoate--beta-alanine ligase]]		[[Category: Pantoate--beta-alanine ligase]]
-	[[Category: Chakrabarti, K S.]]	+	[[Category: Chakrabarti, K S]]
-	[[Category: Gopal, B.]]	+	[[Category: Gopal, B]]
-	[[Category: Sarma, S P.]]	+	[[Category: Sarma, S P]]
-	[[Category: Thakur, K G.]]	+	[[Category: Thakur, K G]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Competitive inhibition]]		[[Category: Competitive inhibition]]

OCA: Protected "3guz" [edit=sysop:move=sysop]

OCA — Wed, 27 Feb 2013 15:40:11 GMT

Protected "3guz" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:40, 27 February 2013

OCA at 15:40, 27 February 2013

OCA — Wed, 27 Feb 2013 15:40:08 GMT

←Older revision		Revision as of 15:40, 27 February 2013
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3guz.png\|left\|200px]]
-	[[Image:3guz.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3guz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3guz\| PDB=3guz \| SCENE= }}		{{STRUCTURE_3guz\| PDB=3guz \| SCENE= }}

	===Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's===		===Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20059543}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20059543 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20059543}}		{{ABSTRACT_PUBMED_20059543}}

	==About this Structure==		==About this Structure==
-	~~3GUZ~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA].	+	[[3guz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA].
		+
		+	==See Also==
		+	*[[Pantothenate synthetase\|Pantothenate synthetase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20059543~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020059543</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Pantoate--beta-alanine ligase]]		[[Category: Pantoate--beta-alanine ligase]]
Line 32:		Line 23:
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Competitive inhibition]]		[[Category: Competitive inhibition]]
-	[[Category: Cytoplasm]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Non-canonical pantoate binding-site]]		[[Category: Non-canonical pantoate binding-site]]
Line 39:		Line 29:
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
	[[Category: Substrate binding]]		[[Category: Substrate binding]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 10 17:22:51 2010''

OCA at 15:22, 10 February 2010

OCA — Wed, 10 Feb 2010 15:22:51 GMT

←Older revision		Revision as of 15:22, 10 February 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3guz.jpg\|left\|200px]]

-	The ~~entry 3guz~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3guz", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3guz\| PDB=3guz \| SCENE= }}

-	~~Authors: Chakrabarti, K.S., Thakur, K.G., Gopal, B., Sarma, S.P.~~	+	===Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's===

-	Description: Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Apr 22 11~~:08:~~52 2009~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20059543}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20059543 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20059543}}
		+
		+	==About this Structure==
		+	3GUZ is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20059543</ref><references group="xtra"/>
		+	[[Category: Escherichia coli]]
		+	[[Category: Pantoate--beta-alanine ligase]]
		+	[[Category: Chakrabarti, K S.]]
		+	[[Category: Gopal, B.]]
		+	[[Category: Sarma, S P.]]
		+	[[Category: Thakur, K G.]]
		+	[[Category: Atp-binding]]
		+	[[Category: Competitive inhibition]]
		+	[[Category: Cytoplasm]]
		+	[[Category: Ligase]]
		+	[[Category: Non-canonical pantoate binding-site]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Pantothenate biosynthesis]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Substrate binding]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 10 17:22:51 2010''

OCA at 08:08, 22 April 2009

OCA — Wed, 22 Apr 2009 08:08:52 GMT

←Older revision		Revision as of 08:08, 22 April 2009
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3guz is ON HOLD	+	The entry 3guz is ON HOLD until Paper Publication

	Authors: Chakrabarti, K.S., Thakur, K.G., Gopal, B., Sarma, S.P.		Authors: Chakrabarti, K.S., Thakur, K.G., Gopal, B., Sarma, S.P.
Line 7:		Line 7:
	Description: Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's		Description: Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr ~~15 09~~:58:04 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 22 11:08:52 2009''