3gwk - Revision history

OCA at 15:45, 1 November 2023

OCA — Wed, 01 Nov 2023 15:45:47 GMT

←Older revision		Revision as of 15:45, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3gwk' size='340' side='right'caption='[[3gwk]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='3gwk' size='340' side='right'caption='[[3gwk]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gwk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Stra5 Stra5~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GWK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gwk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_2603V/R Streptococcus agalactiae 2603V/R]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GWK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[2vrz\|2vrz]], [[2vs0\|2vs0]], [[3fav\|3fav]], [[1wa8\|1wa8]], [[3gvm\|3gvm]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">SAG1039 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208435 STRA5])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gwk OCA], [https://pdbe.org/3gwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gwk RCSB], [https://www.ebi.ac.uk/pdbsum/3gwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gwk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gwk OCA], [https://pdbe.org/3gwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gwk RCSB], [https://www.ebi.ac.uk/pdbsum/3gwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gwk ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ESX2_STRA5 ESX2_STRA5]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Stra5~~]]	+	[[Category: Streptococcus agalactiae 2603V/R]]
-	[[Category: Gries, F]]	+	[[Category: Gries F]]
-	[[Category: Poulsen, C]]	+	[[Category: Poulsen C]]
-	[[Category: Song~~, Y H~~]]	+	[[Category: Song YH]]
-	[[Category: Wilmanns, M]]	+	[[Category: Wilmanns M]]
-	~~[[Category: Four-helical bundle]]~~	+
-	~~[[Category: Viral protein]]~~	+
-	~~[[Category: Wxg motif~~]]	+

OCA at 07:56, 16 March 2022

OCA — Wed, 16 Mar 2022 07:56:46 GMT

←Older revision		Revision as of 07:56, 16 March 2022
Line 1:		Line 1:

	==Structure of the homodimeric WXG-100 family protein from Streptococcus agalactiae==		==Structure of the homodimeric WXG-100 family protein from Streptococcus agalactiae==
-	<StructureSection load='3gwk' size='340' side='right' caption='[[3gwk]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='3gwk' size='340' side='right'caption='[[3gwk]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gwk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Stra5 Stra5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3GWK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gwk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Stra5 Stra5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GWK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vrz\|2vrz]], [[2vs0\|2vs0]], [[3fav\|3fav]], [[1wa8\|1wa8]], [[3gvm\|3gvm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vrz\|2vrz]], [[2vs0\|2vs0]], [[3fav\|3fav]], [[1wa8\|1wa8]], [[3gvm\|3gvm]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG1039 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208435 STRA5])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG1039 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208435 STRA5])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3gwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gwk OCA], [~~http~~://pdbe.org/3gwk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3gwk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3gwk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3gwk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gwk OCA], [https://pdbe.org/3gwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gwk RCSB], [https://www.ebi.ac.uk/pdbsum/3gwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gwk ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Stra5]]		[[Category: Stra5]]
	[[Category: Gries, F]]		[[Category: Gries, F]]

OCA at 05:44, 7 December 2018

OCA — Fri, 07 Dec 2018 05:44:02 GMT

←Older revision		Revision as of 05:44, 7 December 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gw/3gwk_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gw/3gwk_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:58, 11 August 2016

OCA — Thu, 11 Aug 2016 11:58:28 GMT

←Older revision		Revision as of 11:58, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of the homodimeric WXG-100 family protein from Streptococcus agalactiae==		==Structure of the homodimeric WXG-100 family protein from Streptococcus agalactiae==
	<StructureSection load='3gwk' size='340' side='right' caption='[[3gwk]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='3gwk' size='340' side='right' caption='[[3gwk]], [[Resolution\|resolution]] 1.30Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vrz\|2vrz]], [[2vs0\|2vs0]], [[3fav\|3fav]], [[1wa8\|1wa8]], [[3gvm\|3gvm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vrz\|2vrz]], [[2vs0\|2vs0]], [[3fav\|3fav]], [[1wa8\|1wa8]], [[3gvm\|3gvm]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG1039 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208435 STRA5])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG1039 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208435 STRA5])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gwk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gwk RCSB], [http://www.ebi.ac.uk/pdbsum/3gwk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gwk OCA], [http://pdbe.org/3gwk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gwk RCSB], [http://www.ebi.ac.uk/pdbsum/3gwk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gwk ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gwk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3gwk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:42, 5 January 2015

OCA — Mon, 05 Jan 2015 14:42:21 GMT

←Older revision		Revision as of 14:42, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3gwk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Stra5 Stra5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GWK FirstGlance]. <br>		<table><tr><td colspan='2'>[[3gwk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Stra5 Stra5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GWK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vrz\|2vrz]], [[2vs0\|2vs0]], [[3fav\|3fav]], [[1wa8\|1wa8]], [[3gvm\|3gvm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vrz\|2vrz]], [[2vs0\|2vs0]], [[3fav\|3fav]], [[1wa8\|1wa8]], [[3gvm\|3gvm]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG1039 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208435 STRA5])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG1039 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208435 STRA5])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gwk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gwk RCSB], [http://www.ebi.ac.uk/pdbsum/3gwk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gwk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gwk RCSB], [http://www.ebi.ac.uk/pdbsum/3gwk PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Stra5]]		[[Category: Stra5]]
-	[[Category: Gries, F.]]	+	[[Category: Gries, F]]
-	[[Category: Poulsen, C.]]	+	[[Category: Poulsen, C]]
-	[[Category: Song, Y H.]]	+	[[Category: Song, Y H]]
-	[[Category: Wilmanns, M.]]	+	[[Category: Wilmanns, M]]
	[[Category: Four-helical bundle]]		[[Category: Four-helical bundle]]
	[[Category: Viral protein]]		[[Category: Viral protein]]
	[[Category: Wxg motif]]		[[Category: Wxg motif]]

OCA at 06:29, 13 August 2014

OCA — Wed, 13 Aug 2014 06:29:00 GMT

←Older revision		Revision as of 06:29, 13 August 2014
Line 1:		Line 1:
-	[[~~Image:~~3gwk.~~png~~\|~~left~~\|200px]]	+	==Structure of the homodimeric WXG-100 family protein from Streptococcus agalactiae==
		+	<StructureSection load='3gwk' size='340' side='right' caption='[[3gwk]], [[Resolution\|resolution]] 1.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3gwk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Stra5 Stra5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GWK FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vrz\|2vrz]], [[2vs0\|2vs0]], [[3fav\|3fav]], [[1wa8\|1wa8]], [[3gvm\|3gvm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG1039 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208435 STRA5])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gwk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gwk RCSB], [http://www.ebi.ac.uk/pdbsum/3gwk PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gw/3gwk_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Members of the WXG100 protein superfamily form homo- or heterodimeric complexes. The most studied proteins among them are the secreted T-cell antigens CFP-10 (10 kDa culture filtrate protein, EsxB) and ESAT-6 (6 kDa early secreted antigen target, EsxA) from Mycobacterium tuberculosis. They are encoded on an operon within a gene cluster, named as ESX-1, that encodes for the Type VII secretion system (T7SS). WXG100 proteins are secreted in a full-length form and it is known that they adopt a four-helix bundle structure. In the current work we discuss the evolutionary relationship between the homo- and heterodimeric WXG100 proteins, the basis of the oligomeric state and the key structural features of the conserved sequence pattern of WXG100 proteins. We performed an iterative bioinformatics analysis of the WXG100 protein superfamily and correlated this with the atomic structures of the representative WXG100 proteins. We find, firstly, that the WXG100 protein superfamily consists of three subfamilies: CFP-10-, ESAT-6- and sagEsxA-like proteins (EsxA proteins similar to that of Streptococcus agalactiae). Secondly, that the heterodimeric complexes probably evolved from a homodimeric precursor. Thirdly, that the genes of hetero-dimeric WXG100 proteins are always encoded in bi-cistronic operons and finally, by combining the sequence alignments with the X-ray data we identify a conserved C-terminal sequence pattern. The side chains of these conserved residues decorate the same side of the C-terminal alpha-helix and therefore form a distinct surface. Our results lead to a putatively extended T7SS secretion signal which combines two reported T7SS recognition characteristics: Firstly that the T7SS secretion signal is localized at the C-terminus of T7SS substrates and secondly that the conserved residues YxxxD/E are essential for T7SS activity. Furthermore, we propose that the specific alpha-helical surface formed by the conserved sequence pattern including YxxxD/E motif is a key component of T7SS-substrate recognition.

-	~~{{STRUCTURE_3gwk\| PDB=3gwk \| SCENE= }}~~	+	WXG100 protein superfamily consists of three subfamilies and exhibits an alpha-helical C-terminal conserved residue pattern.,Poulsen C, Panjikar S, Holton SJ, Wilmanns M, Song YH PLoS One. 2014 Feb 26;9(2):e89313. doi: 10.1371/journal.pone.0089313. eCollection, 2014. PMID:24586681<ref>PMID:24586681</ref>

-	~~===Structure~~ of the ~~homodimeric WXG-100 family protein from Streptococcus agalactiae===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	==~~About this Structure~~==	+	<references/>
-	~~[[3gwk]] is a 2 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_v Streptococcus agalactiae serogroup v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWK OCA].	+	__TOC__
-	[[Category: ~~Streptococcus agalactiae serogroup v~~]]	+	</StructureSection>
		+	[[Category: Stra5]]
	[[Category: Gries, F.]]		[[Category: Gries, F.]]
	[[Category: Poulsen, C.]]		[[Category: Poulsen, C.]]

OCA: Protected "3gwk" [edit=sysop:move=sysop]

OCA — Wed, 27 Feb 2013 15:16:11 GMT

Protected "3gwk" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:16, 27 February 2013

OCA at 15:16, 27 February 2013

OCA — Wed, 27 Feb 2013 15:16:08 GMT

←Older revision		Revision as of 15:16, 27 February 2013
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-	~~{{Seed}}~~	+	[[Image:3gwk.png\|left\|200px]]
-	[[Image:3gwk.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3gwk", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3gwk\| PDB=3gwk \| SCENE= }}		{{STRUCTURE_3gwk\| PDB=3gwk \| SCENE= }}

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	==About this Structure==		==About this Structure==
-	~~3GWK~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_v Streptococcus agalactiae serogroup v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWK OCA].	+	[[3gwk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_v Streptococcus agalactiae serogroup v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWK OCA].
	[[Category: Streptococcus agalactiae serogroup v]]		[[Category: Streptococcus agalactiae serogroup v]]
	[[Category: Gries, F.]]		[[Category: Gries, F.]]
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	[[Category: Viral protein]]		[[Category: Viral protein]]
	[[Category: Wxg motif]]		[[Category: Wxg motif]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Sep 10 13:55:56 2010''

OCA at 09:47, 10 September 2010

OCA — Fri, 10 Sep 2010 09:47:28 GMT

←Older revision		Revision as of 09:47, 10 September 2010
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3gwk.jpg\|left\|200px]]

-	The ~~entry 3gwk~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3gwk", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3gwk\| PDB=3gwk \| SCENE= }}

-	~~Authors: Poulsen, C., Gries, F., Wilmanns, M., Song, Y.H.~~	+	===Structure of the homodimeric WXG-100 family protein from Streptococcus agalactiae===

-	Description: Structure of the homodimeric WXG-100 family protein from Streptococcus agalactiae

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Sep ~~3 15~~:18:~~46 2009~~''	+	==About this Structure==
		+	3GWK is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_v Streptococcus agalactiae serogroup v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWK OCA].
		+	[[Category: Streptococcus agalactiae serogroup v]]
		+	[[Category: Gries, F.]]
		+	[[Category: Poulsen, C.]]
		+	[[Category: Song, Y H.]]
		+	[[Category: Wilmanns, M.]]
		+	[[Category: Four-helical bundle]]
		+	[[Category: Viral protein]]
		+	[[Category: Wxg motif]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Sep 10 13:55:56 2010''

OCA at 12:18, 3 September 2009

OCA — Thu, 03 Sep 2009 12:18:47 GMT

←Older revision		Revision as of 12:18, 3 September 2009
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	The entry 3gwk is ON HOLD until Paper Publication		The entry 3gwk is ON HOLD until Paper Publication

-	Authors: Poulsen, C~~., Panjikar, S~~., Gries, F., Wilmanns, M., Song, Y.H.	+	Authors: Poulsen, C., Gries, F., Wilmanns, M., Song, Y.H.

	Description: Structure of the homodimeric WXG-100 family protein from Streptococcus agalactiae		Description: Structure of the homodimeric WXG-100 family protein from Streptococcus agalactiae

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Apr~~ 15 09:59:00 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 15:18:46 2009''