3gz1 - Revision history

OCA at 15:46, 1 November 2023

OCA — Wed, 01 Nov 2023 15:46:12 GMT

←Older revision		Revision as of 15:46, 1 November 2023
Line 1:		Line 1:

-	====	+	==Crystal structure of IpgC in complex with the chaperone binding region of IpaB==
-	<StructureSection load='3gz1' size='340' side='right'caption='[[3gz1]]' scene=''>	+	<StructureSection load='3gz1' size='340' side='right'caption='[[3gz1]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gz1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GZ1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gz1 OCA], [https://pdbe.org/3gz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gz1 RCSB], [https://www.ebi.ac.uk/pdbsum/3gz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gz1 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gz1 OCA], [https://pdbe.org/3gz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gz1 RCSB], [https://www.ebi.ac.uk/pdbsum/3gz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gz1 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IPGC_SHIFL IPGC_SHIFL] Assists the correct folding of nascent IpaB. Once it is bound to IpaB, it binds to IpaC and impedes their premature association that would lead to their degradation in the absence of IpcG.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gz1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gz1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The delivery of virulence factors into host cells through type III secretion systems is essential for enterobacterial pathogenesis. Molecular chaperones bind specifically to virulence factors in the bacterial cytosol before secretion. Invasion plasmid gene C (IpgC) is a chaperone that binds 2 essential virulence factors of Shigella: invasion plasmid antigens (Ipa) B and C. Here, we report the crystal structure of IpgC alone and in complex with the chaperone binding domain (CBD) of IpaB. The chaperone captures the CBD in an extended conformation that is stabilized by conserved residues lining the cleft. Analysis of the cocrystal structure reveals a sequence motif that is functional in the IpaB translocator class from different bacteria as determined by isothermal titration calorimetry. Our results show how translocators are chaperoned and may allow the design of inhibitors of enterobacterial diseases.
		+
		+	IpaB-IpgC interaction defines binding motif for type III secretion translocator.,Lunelli M, Lokareddy RK, Zychlinsky A, Kolbe M Proc Natl Acad Sci U S A. 2009 May 28. PMID:19478065<ref>PMID:19478065</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3gz1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Shigella flexneri]]
		+	[[Category: Kolbe M]]
		+	[[Category: Lokareddy RK]]
		+	[[Category: Lunelli M]]
		+	[[Category: Zychlinsky A]]

OCA at 07:42, 10 November 2021

OCA — Wed, 10 Nov 2021 07:42:48 GMT

←Older revision		Revision as of 07:42, 10 November 2021
Line 1:		Line 1:

-	==~~Crystal structure of IpgC in complex with the chaperone binding region of IpaB~~==	+	====
-	<StructureSection load='3gz1' size='340' side='right' caption='[[3gz1]]~~, [[Resolution\|resolution]] 2.15Å~~' scene=''>	+	<StructureSection load='3gz1' size='340' side='right'caption='[[3gz1]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[3gz1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=~~3GZ1~~ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=~~3GZ1~~ FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr>~~<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gz1 OCA], [https://pdbe.org/3gz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gz1 RCSB], [https://www.ebi.ac.uk/pdbsum/3gz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gz1 ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gyz\|3gyz]], [[3gz2\|3gz2]]</td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ipgC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gz1 OCA], [~~http~~://pdbe.org/3gz1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3gz1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3gz1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3gz1 ProSAT]</span></td></tr>	+
	</table>		</table>
-	== Function ==
-	[[http://www.uniprot.org/uniprot/IPGC_SHIFL IPGC_SHIFL]] Assists the correct folding of nascent IpaB. Once it is bound to IpaB, it binds to IpaC and impedes their premature association that would lead to their degradation in the absence of IpcG. [[http://www.uniprot.org/uniprot/IPAB_SHIFL IPAB_SHIFL]] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Forms a pore with IpaC, which is inserted into the host cell membrane through the Mxi/Spa apparatus, during cell contact. This pore probably allows the translocation of IpaA. IpaB has also been found to be necessary and sufficient to activate macrophage apoptosis by binding to interleukin-1 beta converting enzyme (ICE). Has also been shown to be important, along with IpaD, to block or regulate secretion through the Mxi/Spa translocon in the presence or absence of the secretion signal, respectively. Through interaction with host human MAD2L2, constitutively activates the anaphase-promoting complex APC and induces a cell cycle arrest to prevent epithelial renewal in order to promote bacterial colonization.<ref>PMID:17719540</ref> <ref>PMID:9009343</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 16:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gz1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gz1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The delivery of virulence factors into host cells through type III secretion systems is essential for enterobacterial pathogenesis. Molecular chaperones bind specifically to virulence factors in the bacterial cytosol before secretion. Invasion plasmid gene C (IpgC) is a chaperone that binds 2 essential virulence factors of Shigella: invasion plasmid antigens (Ipa) B and C. Here, we report the crystal structure of IpgC alone and in complex with the chaperone binding domain (CBD) of IpaB. The chaperone captures the CBD in an extended conformation that is stabilized by conserved residues lining the cleft. Analysis of the cocrystal structure reveals a sequence motif that is functional in the IpaB translocator class from different bacteria as determined by isothermal titration calorimetry. Our results show how translocators are chaperoned and may allow the design of inhibitors of enterobacterial diseases.
-
-	IpaB-IpgC interaction defines binding motif for type III secretion translocator.,Lunelli M, Lokareddy RK, Zychlinsky A, Kolbe M Proc Natl Acad Sci U S A. 2009 May 28. PMID:19478065<ref>PMID:19478065</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3gz1" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Shigella paradysenteriae weldin 1927~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Kolbe, M]]~~	+	[[Category: Z-disk]]
-	~~[[Category: Lokareddy, R K]]~~	+
-	~~[[Category: Lunelli, M]]~~	+
-	~~[[Category: Zychlinsky, A]]~~	+
-	~~[[Category: Chaperone]]~~	+
-	~~[[Category: Chaperone binding region]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Tetratricopeptide repeat]]~~	+
-	~~[[Category: Tpr]]~~	+
-	~~[[Category: Transmembrane]]~~	+
-	~~[[Category: Virulence~~]]	+

OCA at 05:15, 7 December 2018

OCA — Fri, 07 Dec 2018 05:15:37 GMT

←Older revision		Revision as of 05:15, 7 December 2018
Line 1:		Line 1:
		+
	==Crystal structure of IpgC in complex with the chaperone binding region of IpaB==		==Crystal structure of IpgC in complex with the chaperone binding region of IpaB==
	<StructureSection load='3gz1' size='340' side='right' caption='[[3gz1]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='3gz1' size='340' side='right' caption='[[3gz1]], [[Resolution\|resolution]] 2.15Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gyz\|3gyz]], [[3gz2\|3gz2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gyz\|3gyz]], [[3gz2\|3gz2]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ipgC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ipgC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gz1 OCA], [http://pdbe.org/3gz1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gz1 RCSB], [http://www.ebi.ac.uk/pdbsum/3gz1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gz1 OCA], [http://pdbe.org/3gz1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gz1 RCSB], [http://www.ebi.ac.uk/pdbsum/3gz1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gz1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/3gz1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/3gz1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 00:10, 8 February 2016

OCA — Mon, 08 Feb 2016 00:10:49 GMT

←Older revision		Revision as of 00:10, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='3gz1' size='340' side='right' caption='[[3gz1]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='3gz1' size='340' side='right' caption='[[3gz1]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3gz1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Shigella_flexneri Shigella flexneri~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GZ1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3gz1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GZ1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gyz\|3gyz]], [[3gz2\|3gz2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gyz\|3gyz]], [[3gz2\|3gz2]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ipgC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 Shigella ~~flexneri~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ipgC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gz1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gz1 RCSB], [http://www.ebi.ac.uk/pdbsum/3gz1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gz1 OCA], [http://pdbe.org/3gz1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gz1 RCSB], [http://www.ebi.ac.uk/pdbsum/3gz1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gz1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3gz1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Shigella ~~flexneri~~]]	+	[[Category: Shigella paradysenteriae weldin 1927]]
	[[Category: Kolbe, M]]		[[Category: Kolbe, M]]
	[[Category: Lokareddy, R K]]		[[Category: Lokareddy, R K]]

OCA at 19:10, 24 December 2014

OCA — Wed, 24 Dec 2014 19:10:25 GMT

←Older revision		Revision as of 19:10, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gz1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gz1 RCSB], [http://www.ebi.ac.uk/pdbsum/3gz1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gz1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gz1 RCSB], [http://www.ebi.ac.uk/pdbsum/3gz1 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IPGC_SHIFL IPGC_SHIFL]] Assists the correct folding of nascent IpaB. Once it is bound to IpaB, it binds to IpaC and impedes their premature association that would lead to their degradation in the absence of IpcG. [[http://www.uniprot.org/uniprot/IPAB_SHIFL IPAB_SHIFL]] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Forms a pore with IpaC, which is inserted into the host cell membrane through the Mxi/Spa apparatus, during cell contact. This pore probably allows the translocation of IpaA. IpaB has also been found to be necessary and sufficient to activate macrophage apoptosis by binding to interleukin-1 beta converting enzyme (ICE). Has also been shown to be important, along with IpaD, to block or regulate secretion through the Mxi/Spa translocon in the presence or absence of the secretion signal, respectively. Through interaction with host human MAD2L2, constitutively activates the anaphase-promoting complex APC and induces a cell cycle arrest to prevent epithelial renewal in order to promote bacterial colonization.<ref>PMID:17719540</ref> <ref>PMID:9009343</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:48, 8 December 2014

OCA — Mon, 08 Dec 2014 08:48:34 GMT

←Older revision		Revision as of 08:48, 8 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3gz1.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of IpgC in complex with the chaperone binding region of IpaB==
		+	<StructureSection load='3gz1' size='340' side='right' caption='[[3gz1]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3gz1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GZ1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gyz\|3gyz]], [[3gz2\|3gz2]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ipgC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 Shigella flexneri])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gz1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gz1 RCSB], [http://www.ebi.ac.uk/pdbsum/3gz1 PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/3gz1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The delivery of virulence factors into host cells through type III secretion systems is essential for enterobacterial pathogenesis. Molecular chaperones bind specifically to virulence factors in the bacterial cytosol before secretion. Invasion plasmid gene C (IpgC) is a chaperone that binds 2 essential virulence factors of Shigella: invasion plasmid antigens (Ipa) B and C. Here, we report the crystal structure of IpgC alone and in complex with the chaperone binding domain (CBD) of IpaB. The chaperone captures the CBD in an extended conformation that is stabilized by conserved residues lining the cleft. Analysis of the cocrystal structure reveals a sequence motif that is functional in the IpaB translocator class from different bacteria as determined by isothermal titration calorimetry. Our results show how translocators are chaperoned and may allow the design of inhibitors of enterobacterial diseases.

-	~~{{STRUCTURE_3gz1\| PDB=3gz1 \| SCENE= }}~~	+	IpaB-IpgC interaction defines binding motif for type III secretion translocator.,Lunelli M, Lokareddy RK, Zychlinsky A, Kolbe M Proc Natl Acad Sci U S A. 2009 May 28. PMID:19478065<ref>PMID:19478065</ref>

-	~~===Crystal structure~~ of ~~IpgC in complex with~~ the ~~chaperone binding region of IpaB===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19478065}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3gz1]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Shigella_flexneri Shigella flexneri]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3GZ1 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019478065<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Shigella flexneri]]		[[Category: Shigella flexneri]]
-	[[Category: Kolbe, M.]]	+	[[Category: Kolbe, M]]
-	[[Category: Lokareddy, R K.]]	+	[[Category: Lokareddy, R K]]
-	[[Category: Lunelli, M.]]	+	[[Category: Lunelli, M]]
-	[[Category: Zychlinsky, A.]]	+	[[Category: Zychlinsky, A]]
	[[Category: Chaperone]]		[[Category: Chaperone]]
	[[Category: Chaperone binding region]]		[[Category: Chaperone binding region]]

OCA: Protected "3gz1" [edit=sysop:move=sysop]

OCA — Wed, 27 Feb 2013 15:18:50 GMT

Protected "3gz1" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:18, 27 February 2013

OCA at 15:18, 27 February 2013

OCA — Wed, 27 Feb 2013 15:18:46 GMT

←Older revision		Revision as of 15:18, 27 February 2013
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-	{{Seed}}
	[[Image:3gz1.png\|left\|200px]]		[[Image:3gz1.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3gz1", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3gz1\| PDB=3gz1 \| SCENE= }}		{{STRUCTURE_3gz1\| PDB=3gz1 \| SCENE= }}

	===Crystal structure of IpgC in complex with the chaperone binding region of IpaB===		===Crystal structure of IpgC in complex with the chaperone binding region of IpaB===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19478065}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19478065 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19478065}}		{{ABSTRACT_PUBMED_19478065}}

	==About this Structure==		==About this Structure==
-	~~3GZ1~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZ1 OCA].	+	[[3gz1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZ1 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19478065~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019478065</ref><references group="xtra"/>
	[[Category: Shigella flexneri]]		[[Category: Shigella flexneri]]
	[[Category: Kolbe, M.]]		[[Category: Kolbe, M.]]
Line 31:		Line 19:
	[[Category: Chaperone]]		[[Category: Chaperone]]
	[[Category: Chaperone binding region]]		[[Category: Chaperone binding region]]
-	[[Category: Cytoplasm]]
	[[Category: Membrane]]		[[Category: Membrane]]
-	[[Category: Plasmid]]
	[[Category: Secreted]]		[[Category: Secreted]]
	[[Category: Tetratricopeptide repeat]]		[[Category: Tetratricopeptide repeat]]
Line 39:		Line 25:
	[[Category: Transmembrane]]		[[Category: Transmembrane]]
	[[Category: Virulence]]		[[Category: Virulence]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:25:33 2009''

OCA at 05:25, 25 June 2009

OCA — Thu, 25 Jun 2009 05:25:35 GMT

←Older revision		Revision as of 05:25, 25 June 2009
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	{{Seed}}		{{Seed}}
-	[[Image:3gz1.~~jpg~~\|left\|200px]]	+	[[Image:3gz1.png\|left\|200px]]

	<!--		<!--
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	[[Category: Virulence]]		[[Category: Virulence]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ Jun ~~17 10~~:14:21 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:25:33 2009''

OCA at 07:14, 17 June 2009

OCA — Wed, 17 Jun 2009 07:14:26 GMT

←Older revision		Revision as of 07:14, 17 June 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3gz1.jpg\|left\|200px]]

-	The ~~entry 3gz1~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3gz1", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3gz1\| PDB=3gz1 \| SCENE= }}

-	~~Authors: Lunelli, M., Lokareddy, R.K., Zychlinsky, A., Kolbe, M.~~	+	===Crystal structure of IpgC in complex with the chaperone binding region of IpaB===

-	Description: Crystal structure of IpgC in complex with the chaperone binding region of IpaB

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Apr 15~~ 10:00:11 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19478065}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19478065 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19478065}}
		+
		+	==About this Structure==
		+	3GZ1 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZ1 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19478065</ref><references group="xtra"/>
		+	[[Category: Shigella flexneri]]
		+	[[Category: Kolbe, M.]]
		+	[[Category: Lokareddy, R K.]]
		+	[[Category: Lunelli, M.]]
		+	[[Category: Zychlinsky, A.]]
		+	[[Category: Chaperone]]
		+	[[Category: Chaperone binding region]]
		+	[[Category: Cytoplasm]]
		+	[[Category: Membrane]]
		+	[[Category: Plasmid]]
		+	[[Category: Secreted]]
		+	[[Category: Tetratricopeptide repeat]]
		+	[[Category: Tpr]]
		+	[[Category: Transmembrane]]
		+	[[Category: Virulence]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 17 10:14:21 2009''