3h4h - Revision history

OCA at 11:54, 13 October 2021

OCA — Wed, 13 Oct 2021 11:54:30 GMT

←Older revision		Revision as of 11:54, 13 October 2021
Line 1:		Line 1:

	==Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis==		==Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis==
-	<StructureSection load='3h4h' size='340' side='right' caption='[[3h4h]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='3h4h' size='340' side='right'caption='[[3h4h]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3h4h]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Alcfa Alcfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3H4H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3h4h]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcfa Alcfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H4H FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sjm\|1sjm]], [[3h4f\|3h4f]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1sjm\|1sjm]], [[3h4f\|3h4f]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nir, nirK ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511 ALCFA])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nir, nirK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511 ALCFA])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3h4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4h OCA], [~~http~~://pdbe.org/3h4h PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3h4h RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3h4h PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3h4h ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4h OCA], [https://pdbe.org/3h4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h4h RCSB], [https://www.ebi.ac.uk/pdbsum/3h4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h4h ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Nitrite reductase\|Nitrite reductase]]	+	*[[Nitrite reductase 3D structures\|Nitrite reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Alcfa]]		[[Category: Alcfa]]
		+	[[Category: Large Structures]]
	[[Category: MacPherson, I S]]		[[Category: MacPherson, I S]]
	[[Category: Murphy, I S]]		[[Category: Murphy, I S]]

OCA at 05:48, 7 December 2018

OCA — Fri, 07 Dec 2018 05:48:15 GMT

←Older revision		Revision as of 05:48, 7 December 2018
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/3h4h_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/3h4h_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[~~Nitric~~ reductase\|~~Nitric~~ reductase]]	+	*[[Nitrite reductase\|Nitrite reductase]]
	== References ==		== References ==
	<references/>		<references/>
Line 40:		Line 40:
	[[Category: MacPherson, I S]]		[[Category: MacPherson, I S]]
	[[Category: Murphy, I S]]		[[Category: Murphy, I S]]
		+	[[Category: Copper]]
	[[Category: Fad]]		[[Category: Fad]]
	[[Category: Flavoprotein]]		[[Category: Flavoprotein]]
Line 48:		Line 49:
	[[Category: Oxidase]]		[[Category: Oxidase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
		+	[[Category: Periplasm]]
	[[Category: Pyrrolidone carboxylic acid]]		[[Category: Pyrrolidone carboxylic acid]]

OCA at 19:14, 11 August 2016

OCA — Thu, 11 Aug 2016 19:14:04 GMT

←Older revision		Revision as of 19:14, 11 August 2016
Line 1:		Line 1:
		+
	==Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis==		==Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis==
	<StructureSection load='3h4h' size='340' side='right' caption='[[3h4h]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='3h4h' size='340' side='right' caption='[[3h4h]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3h4h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Alcaligenes_faecalis Alcaligenes faecalis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H4H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3h4h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcfa Alcfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H4H FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sjm\|1sjm]], [[3h4f\|3h4f]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sjm\|1sjm]], [[3h4f\|3h4f]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nir, nirK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511 ~~Alcaligenes faecalis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nir, nirK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511 ALCFA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3h4h RCSB], [http://www.ebi.ac.uk/pdbsum/3h4h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4h OCA], [http://pdbe.org/3h4h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3h4h RCSB], [http://www.ebi.ac.uk/pdbsum/3h4h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3h4h ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h4h ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3h4h" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Alcaligenes faecalis~~]]	+	[[Category: Alcfa]]
	[[Category: MacPherson, I S]]		[[Category: MacPherson, I S]]
	[[Category: Murphy, I S]]		[[Category: Murphy, I S]]

OCA at 09:35, 20 January 2015

OCA — Tue, 20 Jan 2015 09:35:07 GMT

←Older revision		Revision as of 09:35, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3h4h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H4H FirstGlance]. <br>		<table><tr><td colspan='2'>[[3h4h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H4H FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sjm\|1sjm]], [[3h4f\|3h4f]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sjm\|1sjm]], [[3h4f\|3h4f]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nir, nirK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511 Alcaligenes faecalis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nir, nirK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511 Alcaligenes faecalis])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3h4h RCSB], [http://www.ebi.ac.uk/pdbsum/3h4h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3h4h RCSB], [http://www.ebi.ac.uk/pdbsum/3h4h PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Alcaligenes faecalis]]		[[Category: Alcaligenes faecalis]]
-	[[Category: MacPherson, I S.]]	+	[[Category: MacPherson, I S]]
-	[[Category: Murphy, I S.]]	+	[[Category: Murphy, I S]]
	[[Category: Fad]]		[[Category: Fad]]
	[[Category: Flavoprotein]]		[[Category: Flavoprotein]]

OCA at 13:20, 29 September 2014

OCA — Mon, 29 Sep 2014 13:20:54 GMT

←Older revision		Revision as of 13:20, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3h4h.~~png~~\|~~left~~\|200px]]	+	==Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis==
		+	<StructureSection load='3h4h' size='340' side='right' caption='[[3h4h]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3h4h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H4H FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sjm\|1sjm]], [[3h4f\|3h4f]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nir, nirK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511 Alcaligenes faecalis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3h4h RCSB], [http://www.ebi.ac.uk/pdbsum/3h4h PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/3h4h_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Directed evolution methods were developed for Cu-containing nitrite reductase (NiR) from Alcaligenes faecalis S-6. The PCR cloning strategy allows for the efficient production of libraries of 100 000 clones by a modification of a megaprimer-based whole-plasmid synthesis reaction. The high-throughput screen includes colony lift onto a nylon membrane and subsequent lysis of NiR-expressing colonies in the presence of Cu(2+) ions for copper incorporation into intracellularly expressed NiR. Addition of a chromogenic substrate, 3, 3'-diaminobenzidine (DAB), results in deposition of red, insoluble color at the site of oxidation by functional NiR. Twenty-thousand random variants of NiR were screened for improved function with DAB as a reductant, and five variants were identified. These variants were shuffled and screened, yielding two double variants. An analog of the DAB substrate, o-dianisidine, which is oxidized to a water-soluble product was used for functional characterization. The double variant M150L/F312C was most proficient at o-dianisidine oxidation with dioxygen as the electron acceptor (5.5X wt), and the M150L single variant was most proficient at o-dianisidine oxidation with nitrite as the electron acceptor (8.5X wt). The library generation and screening method can be employed for evolving new reductase functions in NiR and for screening of efficient folding of engineered NiRs.

-	~~{{STRUCTURE_3h4h\| PDB=3h4h \| SCENE= }}~~	+	Directed evolution of copper nitrite reductase to a chromogenic reductant.,MacPherson IS, Rosell FI, Scofield M, Mauk AG, Murphy ME Protein Eng Des Sel. 2010 Mar;23(3):137-45. Epub 2010 Jan 18. PMID:20083495<ref>PMID:20083495</ref>

-	~~===Met94Thr~~/~~Phe312Cys variant~~ of ~~nitrite reductase from Alcaligenes faecalis===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20083495}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3h4h]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA].~~	+

	==See Also==		==See Also==
	*[[Nitric reductase\|Nitric reductase]]		*[[Nitric reductase\|Nitric reductase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020083495<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Alcaligenes faecalis]]		[[Category: Alcaligenes faecalis]]
	[[Category: MacPherson, I S.]]		[[Category: MacPherson, I S.]]

OCA at 17:33, 20 October 2012

OCA — Sat, 20 Oct 2012 17:33:09 GMT

←Older revision		Revision as of 17:33, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3h4h]] is a 3 chain structure ~~of [[Nitric reductase]]~~ with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA].	+	[[3h4h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA].

	==See Also==		==See Also==

OCA: Protected "3h4h" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 01:16:28 GMT

Protected "3h4h" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:16, 27 July 2012

OCA at 01:16, 27 July 2012

OCA — Fri, 27 Jul 2012 01:16:26 GMT

←Older revision		Revision as of 01:16, 27 July 2012
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-	{{Seed}}
	[[Image:3h4h.png\|left\|200px]]		[[Image:3h4h.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3h4h", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3h4h\| PDB=3h4h \| SCENE= }}		{{STRUCTURE_3h4h\| PDB=3h4h \| SCENE= }}

	===Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis===		===Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20083495}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20083495 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20083495}}		{{ABSTRACT_PUBMED_20083495}}

	==About this Structure==		==About this Structure==
-	~~3H4H~~ is a 3 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA].	+	[[3h4h]] is a 3 chain structure of [[Nitric reductase]] with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA].
		+
		+	==See Also==
		+	*[[Nitric reductase\|Nitric reductase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20083495~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020083495</ref><references group="xtra"/>
	[[Category: Alcaligenes faecalis]]		[[Category: Alcaligenes faecalis]]
	[[Category: MacPherson, I S.]]		[[Category: MacPherson, I S.]]
	[[Category: Murphy, I S.]]		[[Category: Murphy, I S.]]
-	[[Category: Copper]]
	[[Category: Fad]]		[[Category: Fad]]
	[[Category: Flavoprotein]]		[[Category: Flavoprotein]]
Line 36:		Line 26:
	[[Category: Oxidase]]		[[Category: Oxidase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-	[[Category: Periplasm]]
	[[Category: Pyrrolidone carboxylic acid]]		[[Category: Pyrrolidone carboxylic acid]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 17 09:55:14 2010''

OCA at 07:55, 17 February 2010

OCA — Wed, 17 Feb 2010 07:55:14 GMT

←Older revision		Revision as of 07:55, 17 February 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3h4h.~~jpg~~\|left\|200px]]	+	[[Image:3h4h.png\|left\|200px]]

	<!--		<!--
Line 39:		Line 39:
	[[Category: Pyrrolidone carboxylic acid]]		[[Category: Pyrrolidone carboxylic acid]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 3 09:42:21 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 17 09:55:14 2010''

OCA at 07:42, 3 February 2010

OCA — Wed, 03 Feb 2010 07:42:22 GMT

←Older revision		Revision as of 07:42, 3 February 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3h4h.jpg\|left\|200px]]

-	The ~~entry 3h4h~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3h4h", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3h4h\| PDB=3h4h \| SCENE= }}

-	~~Authors: MacPherson, I.S., Murphy, I.S.~~	+	===Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis===

-	Description: Met94Thr/Phe312Cys variant of nitrite reductase from Alcaligenes faecalis

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jul~~ ~~1 08~~:55:~~14 2009~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20083495}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20083495 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20083495}}
		+
		+	==About this Structure==
		+	3H4H is a 3 chains structure with sequences from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4H OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20083495</ref><references group="xtra"/>
		+	[[Category: Alcaligenes faecalis]]
		+	[[Category: MacPherson, I S.]]
		+	[[Category: Murphy, I S.]]
		+	[[Category: Copper]]
		+	[[Category: Fad]]
		+	[[Category: Flavoprotein]]
		+	[[Category: High-throuhput screening]]
		+	[[Category: Metal-binding]]
		+	[[Category: Nitrate assimilation]]
		+	[[Category: Nitrite reductase]]
		+	[[Category: Oxidase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Periplasm]]
		+	[[Category: Pyrrolidone carboxylic acid]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 3 09:42:21 2010''