3h72 - Revision history

OCA at 09:58, 21 February 2024

OCA — Wed, 21 Feb 2024 09:58:19 GMT

←Older revision		Revision as of 09:58, 21 February 2024
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	<StructureSection load='3h72' size='340' side='right'caption='[[3h72]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3h72' size='340' side='right'caption='[[3h72]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3h72]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Strr6 Strr6~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H72 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3h72]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_R6 Streptococcus pneumoniae R6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H72 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SIA:O~~-~~SIALIC+ACID'>SIA</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3h6j\|3h6j]], [[3h71\|3h71]], [[3h73\|3h73]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>nanA, spr1536 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~171101 STRR6])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h72 OCA], [https://pdbe.org/3h72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h72 RCSB], [https://www.ebi.ac.uk/pdbsum/3h72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h72 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h72 OCA], [https://pdbe.org/3h72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h72 RCSB], [https://www.ebi.ac.uk/pdbsum/3h72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h72 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NANA_STRR6 NANA_STRR6]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h72 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h72 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Currently there is pressing need to develop novel therapeutic agents for the treatment of infections by the human respiratory pathogens Pseudomonas aeruginosa and Streptococcus pneumoniae. The neuraminidases of these pathogens are important for host colonization in animal models of infection and are attractive targets for drug discovery. To aid in the development of inhibitors against these neuraminidases, we have determined the crystal structures of the P. aeruginosa enzyme NanPs and S. pneumoniae enzyme NanA at 1.6 and 1.7A resolution, respectively. In situ proteolysis with trypsin was essential for the crystallization of our recombinant NanA. The active site regions of the two enzymes are strikingly different. NanA contains a deep pocket that is similar to that in canonical neuraminidases, while the NanPs active site is much more open. The comparative studies suggest that NanPs may not be a classical neuraminidase, and may have distinct natural substrates and physiological functions. This work represents an important step in the development of drugs to prevent respiratory tract colonization by these two pathogens.
-
-	Crystal structures of respiratory pathogen neuraminidases.,Hsiao YS, Parker D, Ratner AJ, Prince A, Tong L Biochem Biophys Res Commun. 2009 Mar 13;380(3):467-71. Epub 2009 Jan 23. PMID:19284989<ref>PMID:19284989</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3h72" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Neuraminidase 3D structures\|Neuraminidase 3D structures]]		*[[Neuraminidase 3D structures\|Neuraminidase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Exo-alpha-sialidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Strr6~~]]	+	[[Category: Streptococcus pneumoniae R6]]
-	[[Category: Hsiao, Y S]]	+	[[Category: Hsiao Y-S]]
-	[[Category: Tong, L]]	+	[[Category: Tong L]]
-	~~[[Category: Cell wall]]~~	+
-	~~[[Category: Glycosidase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Peptidoglycan-anchor]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Six-bladed beta-propeller~~]]	+

OCA at 08:04, 16 March 2022

OCA — Wed, 16 Mar 2022 08:04:57 GMT

←Older revision		Revision as of 08:04, 16 March 2022
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	==Crystal structure of Streptococcus pneumoniae D39 neuraminidase A precursor (NanA) in complex with NANA==		==Crystal structure of Streptococcus pneumoniae D39 neuraminidase A precursor (NanA) in complex with NANA==
-	<StructureSection load='3h72' size='340' side='right' caption='[[3h72]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='3h72' size='340' side='right'caption='[[3h72]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3h72]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Strr6 Strr6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3H72 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3h72]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Strr6 Strr6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H72 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3h6j\|3h6j]], [[3h71\|3h71]], [[3h73\|3h73]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3h6j\|3h6j]], [[3h71\|3h71]], [[3h73\|3h73]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nanA, spr1536 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=171101 STRR6])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nanA, spr1536 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=171101 STRR6])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3h72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h72 OCA], [~~http~~://pdbe.org/3h72 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3h72 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3h72 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3h72 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h72 OCA], [https://pdbe.org/3h72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h72 RCSB], [https://www.ebi.ac.uk/pdbsum/3h72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h72 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h7/3h72_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h7/3h72_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 3h72" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3h72" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Neuraminidase 3D structures\|Neuraminidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Exo-alpha-sialidase]]		[[Category: Exo-alpha-sialidase]]
		+	[[Category: Large Structures]]
	[[Category: Strr6]]		[[Category: Strr6]]
	[[Category: Hsiao, Y S]]		[[Category: Hsiao, Y S]]

OCA at 07:23, 1 November 2017

OCA — Wed, 01 Nov 2017 07:23:30 GMT

←Older revision		Revision as of 07:23, 1 November 2017
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 3h72" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3h72" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Neuraminidase\|Neuraminidase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:39, 11 August 2016

OCA — Thu, 11 Aug 2016 19:39:09 GMT

←Older revision		Revision as of 19:39, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Streptococcus pneumoniae D39 neuraminidase A precursor (NanA) in complex with NANA==		==Crystal structure of Streptococcus pneumoniae D39 neuraminidase A precursor (NanA) in complex with NANA==
	<StructureSection load='3h72' size='340' side='right' caption='[[3h72]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3h72' size='340' side='right' caption='[[3h72]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3h72]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_pneumoniae Streptococcus pneumoniae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H72 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3h72]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strr6 Strr6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H72 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3h6j\|3h6j]], [[3h71\|3h71]], [[3h73\|3h73]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3h6j\|3h6j]], [[3h71\|3h71]], [[3h73\|3h73]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nanA, spr1536 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1313 Streptococcus pneumoniae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nanA, spr1536 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=171101 STRR6])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3h72 RCSB], [http://www.ebi.ac.uk/pdbsum/3h72 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h72 OCA], [http://pdbe.org/3h72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3h72 RCSB], [http://www.ebi.ac.uk/pdbsum/3h72 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3h72 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h72 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3h72" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Exo-alpha-sialidase]]		[[Category: Exo-alpha-sialidase]]
-	[[Category: ~~Streptococcus pneumoniae~~]]	+	[[Category: Strr6]]
	[[Category: Hsiao, Y S]]		[[Category: Hsiao, Y S]]
	[[Category: Tong, L]]		[[Category: Tong, L]]

OCA at 10:03, 20 January 2015

OCA — Tue, 20 Jan 2015 10:03:23 GMT

←Older revision		Revision as of 10:03, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3h72]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H72 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3h72]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H72 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3h6j\|3h6j]], [[3h71\|3h71]], [[3h73\|3h73]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3h6j\|3h6j]], [[3h71\|3h71]], [[3h73\|3h73]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nanA, spr1536 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nanA, spr1536 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3h72 RCSB], [http://www.ebi.ac.uk/pdbsum/3h72 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3h72 RCSB], [http://www.ebi.ac.uk/pdbsum/3h72 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Exo-alpha-sialidase]]		[[Category: Exo-alpha-sialidase]]
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]
-	[[Category: Hsiao, Y S.]]	+	[[Category: Hsiao, Y S]]
-	[[Category: Tong, L.]]	+	[[Category: Tong, L]]
	[[Category: Cell wall]]		[[Category: Cell wall]]
	[[Category: Glycosidase]]		[[Category: Glycosidase]]

OCA at 12:13, 29 September 2014

OCA — Mon, 29 Sep 2014 12:13:02 GMT

←Older revision		Revision as of 12:13, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3h72.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of Streptococcus pneumoniae D39 neuraminidase A precursor (NanA) in complex with NANA==
		+	<StructureSection load='3h72' size='340' side='right' caption='[[3h72]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3h72]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H72 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3h6j\|3h6j]], [[3h71\|3h71]], [[3h73\|3h73]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nanA, spr1536 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3h72 RCSB], [http://www.ebi.ac.uk/pdbsum/3h72 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h7/3h72_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Currently there is pressing need to develop novel therapeutic agents for the treatment of infections by the human respiratory pathogens Pseudomonas aeruginosa and Streptococcus pneumoniae. The neuraminidases of these pathogens are important for host colonization in animal models of infection and are attractive targets for drug discovery. To aid in the development of inhibitors against these neuraminidases, we have determined the crystal structures of the P. aeruginosa enzyme NanPs and S. pneumoniae enzyme NanA at 1.6 and 1.7A resolution, respectively. In situ proteolysis with trypsin was essential for the crystallization of our recombinant NanA. The active site regions of the two enzymes are strikingly different. NanA contains a deep pocket that is similar to that in canonical neuraminidases, while the NanPs active site is much more open. The comparative studies suggest that NanPs may not be a classical neuraminidase, and may have distinct natural substrates and physiological functions. This work represents an important step in the development of drugs to prevent respiratory tract colonization by these two pathogens.

-	~~{{STRUCTURE_3h72\| PDB=3h72 \| SCENE= }}~~	+	Crystal structures of respiratory pathogen neuraminidases.,Hsiao YS, Parker D, Ratner AJ, Prince A, Tong L Biochem Biophys Res Commun. 2009 Mar 13;380(3):467-71. Epub 2009 Jan 23. PMID:19284989<ref>PMID:19284989</ref>

-	~~===Crystal structure~~ of ~~Streptococcus pneumoniae D39 neuraminidase A precursor (NanA) in complex with NANA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19284989}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3h72]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA].~~	+

	==See Also==		==See Also==
	*[[Neuraminidase\|Neuraminidase]]		*[[Neuraminidase\|Neuraminidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019284989<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Exo-alpha-sialidase]]		[[Category: Exo-alpha-sialidase]]
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]

OCA at 17:41, 20 October 2012

OCA — Sat, 20 Oct 2012 17:41:06 GMT

←Older revision		Revision as of 17:41, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3h72]] is a 2 chain structure ~~of [[Neuraminidase]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA].	+	[[3h72]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA].

	==See Also==		==See Also==

OCA: Protected "3h72" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 01:41:43 GMT

Protected "3h72" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:41, 27 July 2012

OCA at 01:41, 27 July 2012

OCA — Fri, 27 Jul 2012 01:41:41 GMT

←Older revision		Revision as of 01:41, 27 July 2012
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3h72.png\|left\|200px]]
-	[[Image:3h72.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3h72", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3h72\| PDB=3h72 \| SCENE= }}		{{STRUCTURE_3h72\| PDB=3h72 \| SCENE= }}

	===Crystal structure of Streptococcus pneumoniae D39 neuraminidase A precursor (NanA) in complex with NANA===		===Crystal structure of Streptococcus pneumoniae D39 neuraminidase A precursor (NanA) in complex with NANA===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19284989}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19284989 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19284989}}		{{ABSTRACT_PUBMED_19284989}}

	==About this Structure==		==About this Structure==
-	~~3H72~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA].	+	[[3h72]] is a 2 chain structure of [[Neuraminidase]] with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA].
		+
		+	==See Also==
		+	*[[Neuraminidase\|Neuraminidase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19284989~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019284989</ref><references group="xtra"/>
	[[Category: Exo-alpha-sialidase]]		[[Category: Exo-alpha-sialidase]]
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]
Line 34:		Line 25:
	[[Category: Secreted]]		[[Category: Secreted]]
	[[Category: Six-bladed beta-propeller]]		[[Category: Six-bladed beta-propeller]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 13 10:07:41 2009''

OCA at 07:07, 13 May 2009

OCA — Wed, 13 May 2009 07:07:42 GMT

←Older revision		Revision as of 07:07, 13 May 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3h72.jpg\|left\|200px]]

-	The ~~entry 3h72~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3h72", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3h72\| PDB=3h72 \| SCENE= }}

-	~~Authors: Hsiao, Y.-S., Tong, L.~~	+	===Crystal structure of Streptococcus pneumoniae D39 neuraminidase A precursor (NanA) in complex with NANA===

-	Description: Crystal structure of Streptococcus pneumoniae D39 neuraminidase A precursor (NanA) in complex with NANA

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May ~~6 09~~:41:40 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19284989}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19284989 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19284989}}
		+
		+	==About this Structure==
		+	3H72 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H72 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19284989</ref><references group="xtra"/>
		+	[[Category: Exo-alpha-sialidase]]
		+	[[Category: Streptococcus pneumoniae]]
		+	[[Category: Hsiao, Y S.]]
		+	[[Category: Tong, L.]]
		+	[[Category: Cell wall]]
		+	[[Category: Glycosidase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Peptidoglycan-anchor]]
		+	[[Category: Secreted]]
		+	[[Category: Six-bladed beta-propeller]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 13 10:07:41 2009''