3hbq - Revision history

OCA at 07:19, 6 September 2023

OCA — Wed, 06 Sep 2023 07:19:31 GMT

←Older revision		Revision as of 07:19, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3hbq' size='340' side='right'caption='[[3hbq]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3hbq' size='340' side='right'caption='[[3hbq]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hbq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chick Chick~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HBQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HBQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hbq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HBQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HBQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MOM:HYDROXY(DIOXO)MOLYBDENUM'>MOM</scene>, <scene name='pdbligand=MTE:PHOSPHONIC+ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER'>MTE</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1sox\|1sox]], [[2a99\|2a99]], [[2a9a\|2a9a]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MOM:HYDROXY(DIOXO)MOLYBDENUM'>MOM</scene>, <scene name='pdbligand=MTE:PHOSPHONIC+ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER'>MTE</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sulfite oxidase ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hbq OCA], [https://pdbe.org/3hbq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hbq RCSB], [https://www.ebi.ac.uk/pdbsum/3hbq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hbq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hbq OCA], [https://pdbe.org/3hbq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hbq RCSB], [https://www.ebi.ac.uk/pdbsum/3hbq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hbq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SUOX_CHICK SUOX_CHICK]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chick~~]]	+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Qiu~~, J A]]~~	+	[[Category: Qiu JA]]
-	~~[[Category: Metal binding]]~~	+
-	~~[[Category: Molybdenum]]~~	+
-	~~[[Category: Molybdopterin]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Oxotransferase]]~~	+
-	~~[[Category: Sulfite oxidase~~]]	+

OCA at 08:08, 16 March 2022

OCA — Wed, 16 Mar 2022 08:08:17 GMT

←Older revision		Revision as of 08:08, 16 March 2022
Line 1:		Line 1:

	==Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala==		==Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala==
-	<StructureSection load='3hbq' size='340' side='right' caption='[[3hbq]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='3hbq' size='340' side='right'caption='[[3hbq]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hbq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HBQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3HBQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hbq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HBQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HBQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MOM:HYDROXY(DIOXO)MOLYBDENUM'>MOM</scene>, <scene name='pdbligand=MTE:PHOSPHONIC+ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER'>MTE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MOM:HYDROXY(DIOXO)MOLYBDENUM'>MOM</scene>, <scene name='pdbligand=MTE:PHOSPHONIC+ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER'>MTE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sox\|1sox]], [[2a99\|2a99]], [[2a9a\|2a9a]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1sox\|1sox]], [[2a99\|2a99]], [[2a9a\|2a9a]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sulfite oxidase ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sulfite oxidase ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3hbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hbq OCA], [~~http~~://pdbe.org/3hbq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3hbq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3hbq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3hbq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hbq OCA], [https://pdbe.org/3hbq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hbq RCSB], [https://www.ebi.ac.uk/pdbsum/3hbq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hbq ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hb/3hbq_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hb/3hbq_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 3hbq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3hbq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Sulfite Oxidase\|Sulfite Oxidase]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Chick]]		[[Category: Chick]]
		+	[[Category: Large Structures]]
	[[Category: Qiu, J A]]		[[Category: Qiu, J A]]
	[[Category: Metal binding]]		[[Category: Metal binding]]

OCA at 07:27, 1 November 2017

OCA — Wed, 01 Nov 2017 07:27:57 GMT

←Older revision		Revision as of 07:27, 1 November 2017
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 3hbq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3hbq" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Sulfite Oxidase\|Sulfite Oxidase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:56, 5 August 2016

OCA — Fri, 05 Aug 2016 12:56:32 GMT

←Older revision		Revision as of 12:56, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala==		==Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala==
	<StructureSection load='3hbq' size='340' side='right' caption='[[3hbq]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3hbq' size='340' side='right' caption='[[3hbq]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hbq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Gallus_gallus Gallus gallus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HBQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HBQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hbq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HBQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HBQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MOM:HYDROXY(DIOXO)MOLYBDENUM'>MOM</scene>, <scene name='pdbligand=MTE:PHOSPHONIC+ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER'>MTE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MOM:HYDROXY(DIOXO)MOLYBDENUM'>MOM</scene>, <scene name='pdbligand=MTE:PHOSPHONIC+ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER'>MTE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sox\|1sox]], [[2a99\|2a99]], [[2a9a\|2a9a]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sox\|1sox]], [[2a99\|2a99]], [[2a9a\|2a9a]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sulfite oxidase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 ~~Gallus gallus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sulfite oxidase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hbq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hbq RCSB], [http://www.ebi.ac.uk/pdbsum/3hbq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hbq OCA], [http://pdbe.org/3hbq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hbq RCSB], [http://www.ebi.ac.uk/pdbsum/3hbq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hbq ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hbq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3hbq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Gallus gallus~~]]	+	[[Category: Chick]]
	[[Category: Qiu, J A]]		[[Category: Qiu, J A]]
	[[Category: Metal binding]]		[[Category: Metal binding]]

OCA at 05:57, 18 December 2014

OCA — Thu, 18 Dec 2014 05:57:58 GMT

←Older revision		Revision as of 05:57, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3hbq\| PDB=3hbq \| SCENE= }}~~	+	==Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala==
-	===Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala===	+	<StructureSection load='3hbq' size='340' side='right' caption='[[3hbq]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20356030}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3hbq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HBQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HBQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MOM:HYDROXY(DIOXO)MOLYBDENUM'>MOM</scene>, <scene name='pdbligand=MTE:PHOSPHONIC+ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER'>MTE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sox\|1sox]], [[2a99\|2a99]], [[2a9a\|2a9a]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sulfite oxidase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hbq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hbq RCSB], [http://www.ebi.ac.uk/pdbsum/3hbq PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hb/3hbq_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sulfite oxidase (SO) catalyzes the physiologically critical conversion of sulfite to sulfate. Enzymatic activity is dependent on the presence of the metal molybdenum complexed with a pyrano-pterin-dithiolene cofactor termed molybdopterin. Comparison of the amino acid sequences of SOs from a variety of sources has identified a single conserved Cys residue essential for catalytic activity. The crystal structure of chicken liver sulfite oxidase indicated that this residue, Cys185 in Chicken SO, coordinates the Mo atom in the active site. To better understand the role of this residue in the catalytic mechanism of sulfite oxidase, serine and alanine variants at position 185 of recombinant chicken SO were generated. Spectroscopic and kinetic studies indicate that neither variant is capable of sulfite oxidation. The crystal structure of the C185S variant was solved to 1.9 A resolution, to 2.4 A resolution in the presence of sulfite, and the C185A variant to 2.8 A resolution. The structures of the C185S and C185A variants revealed that neither the Ser or Ala side chains appeared to closely interact with the Mo atom and that a third oxo group replaced the usual cysteine sulfur ligand at the Mo center, confirming earlier EXAFS work on the human C207S mutant. An unexpected result was that in the C185S variant, in the absence of sulfite, the active site residue Tyr322 became disordered as did the loop region flanking it. In the C185S variant crystallized in the presence of sulfite, the Tyr322 residue re-localized to the active site. The C185A variant structure also indicated the presence of a third oxygen ligand, however Tyr322 remained in the active site. EXAFS studies of the Mo coordination environment indicate the Mo atom is in the oxidized MoVI state in both the C185S and C185A variants of chicken SO and show the expected trioxo dithiolene active site. Density functional theory calculations of the trioxo form of the cofactor reasonably reproducd the Mo=O distances of the complex, however the calculated MoS distances were slightly longer than either crystallographic or EXAFS measurements. Taken together, these results indicate that the active sites of the C185S and C185A variants are essentially catalytically inactive, the crystal structures of C185S and C185A variants contain a fully oxidized, trioxo form of the cofactor, and Tyr322 can undergo a conformational change which is of relevance to the reaction mechanism. Additional DFT calculations demonstrated that such methods can reasonably reproduce the geometry and bond lengths of the active site.

-	~~==About this Structure==~~	+	The Structures of the C185S and C185A Mutants of Sulfite Oxidase Reveal Rearrangement of the Active Site.,Qiu JA, Wilson HL, Pushie MJ, Kisker CF, George GN, Rajagopalan KV Biochemistry. 2010 Mar 31. PMID:20356030<ref>PMID:20356030</ref>
-	~~[[3hbq]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Gallus_gallus Gallus gallus]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3HBQ OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Sulfite Oxidase\|Sulfite Oxidase]]		*[[Sulfite Oxidase\|Sulfite Oxidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020356030<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
-	[[Category: Qiu, J A.]]	+	[[Category: Qiu, J A]]
	[[Category: Metal binding]]		[[Category: Metal binding]]
	[[Category: Molybdenum]]		[[Category: Molybdenum]]

OCA at 09:06, 11 March 2013

OCA — Mon, 11 Mar 2013 09:06:09 GMT

←Older revision		Revision as of 09:06, 11 March 2013
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-	[[Image:3hbq.png\|left\|200px]]
-
	{{STRUCTURE_3hbq\| PDB=3hbq \| SCENE= }}		{{STRUCTURE_3hbq\| PDB=3hbq \| SCENE= }}
-
	===Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala===		===Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala===
-
	{{ABSTRACT_PUBMED_20356030}}		{{ABSTRACT_PUBMED_20356030}}

OCA: Protected "3hbq" [edit=sysop:move=sysop]

OCA — Wed, 06 Mar 2013 14:38:06 GMT

Protected "3hbq" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:38, 6 March 2013

OCA at 14:38, 6 March 2013

OCA — Wed, 06 Mar 2013 14:38:02 GMT

←Older revision		Revision as of 14:38, 6 March 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3hbq.png\|left\|200px]]		[[Image:3hbq.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3hbq", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3hbq\| PDB=3hbq \| SCENE= }}		{{STRUCTURE_3hbq\| PDB=3hbq \| SCENE= }}

	===Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala===		===Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20356030}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20356030 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20356030}}		{{ABSTRACT_PUBMED_20356030}}

	==About this Structure==		==About this Structure==
-	~~3HBQ~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HBQ OCA].	+	[[3hbq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HBQ OCA].
		+
		+	==See Also==
		+	*[[Sulfite Oxidase\|Sulfite Oxidase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20356030~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020356030</ref><references group="xtra"/>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Qiu, J A.]]		[[Category: Qiu, J A.]]
Line 32:		Line 23:
	[[Category: Oxotransferase]]		[[Category: Oxotransferase]]
	[[Category: Sulfite oxidase]]		[[Category: Sulfite oxidase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 20 08:57:09 2010''

OCA at 05:57, 20 May 2010

OCA — Thu, 20 May 2010 05:57:10 GMT

←Older revision		Revision as of 05:57, 20 May 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3hbq.~~jpg~~\|left\|200px]]	+	[[Image:3hbq.png\|left\|200px]]

	<!--		<!--
Line 33:		Line 33:
	[[Category: Sulfite oxidase]]		[[Category: Sulfite oxidase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Apr 14 09~~:33:59 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 20 08:57:09 2010''

OCA at 06:34, 14 April 2010

OCA — Wed, 14 Apr 2010 06:34:00 GMT

←Older revision		Revision as of 06:34, 14 April 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3hbq.jpg\|left\|200px]]

-	The ~~entry 3hbq~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3hbq", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3hbq\| PDB=3hbq \| SCENE= }}

-	~~Authors: Qiu, J.A.~~	+	===Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala===

-	Description: Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~May 27~~ 14:39:~~21 2009~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20356030}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20356030 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20356030}}
		+
		+	==About this Structure==
		+	3HBQ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HBQ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20356030</ref><references group="xtra"/>
		+	[[Category: Gallus gallus]]
		+	[[Category: Qiu, J A.]]
		+	[[Category: Metal binding]]
		+	[[Category: Molybdenum]]
		+	[[Category: Molybdopterin]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Oxotransferase]]
		+	[[Category: Sulfite oxidase]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 14 09:33:59 2010''