3hiq - Revision history

OCA at 10:00, 21 February 2024

OCA — Wed, 21 Feb 2024 10:00:17 GMT

←Older revision		Revision as of 10:00, 21 February 2024
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	<StructureSection load='3hiq' size='340' side='right'caption='[[3hiq]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='3hiq' size='340' side='right'caption='[[3hiq]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hiq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Common_soapwort Common soapwort~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HIQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hiq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saponaria_officinalis Saponaria officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HIQ FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[3hio\|3hio]]~~, [[~~3his~~\|~~3his~~]]~~, [[3hit\|3hit]], [[3hiv\|3hiv]], [[3hiw\|3hiw]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.19Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.~~2.22 3.2.2.22] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hiq OCA], [https://pdbe.org/3hiq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hiq RCSB], [https://www.ebi.ac.uk/pdbsum/3hiq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hiq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hiq OCA], [https://pdbe.org/3hiq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hiq RCSB], [https://www.ebi.ac.uk/pdbsum/3hiq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hiq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q2QEH4_SAPOF Q2QEH4_SAPOF]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hiq ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hiq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Ricin A-chain (RTA) and saporin-L1 (SAP) catalyze adenosine depurination of 28S rRNA to inhibit protein synthesis and cause cell death. We present the crystal structures of RTA and SAP in complex with transition state analogue inhibitors. These tight-binding inhibitors mimic the sarcin-ricin recognition loop of 28S rRNA and the dissociative ribocation transition state established for RTA catalysis. RTA and SAP share unique purine-binding geometry with quadruple pi-stacking interactions between adjacent adenine and guanine bases and 2 conserved tyrosines. An arginine at one end of the pi-stack provides cationic polarization and enhanced leaving group ability to the susceptible adenine. Common features of these ribosome-inactivating proteins include adenine leaving group activation, a remarkable lack of ribocation stabilization, and conserved glutamates as general bases for activation of the H(2)O nucleophile. Catalytic forces originate primarily from leaving group activation evident in both RTA and SAP in complex with transition state analogues.
-
-	Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins.,Ho MC, Sturm MB, Almo SC, Schramm VL Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20276-81. Epub 2009 Nov 17. PMID:19920175<ref>PMID:19920175</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3hiq" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Common soapwort]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~RRNA N-glycosylase~~]]	+	[[Category: Saponaria officinalis]]
-	[[Category: Almo~~, S C~~]]	+	[[Category: Almo SC]]
-	[[Category: Ho, M]]	+	[[Category: Ho M]]
-	[[Category: Schramm~~, V L~~]]	+	[[Category: Schramm VL]]
-	[[Category: Sturm~~, M B]]~~	+	[[Category: Sturm MB]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Plant defense]]~~	+
-	~~[[Category: Protein synthesis inhibitor]]~~	+
-	~~[[Category: Ribosome inactivating protein]]~~	+
-	~~[[Category: Rip]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 12:00, 13 October 2021

OCA — Wed, 13 Oct 2021 12:00:04 GMT

←Older revision		Revision as of 12:00, 13 October 2021
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	<StructureSection load='3hiq' size='340' side='right'caption='[[3hiq]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='3hiq' size='340' side='right'caption='[[3hiq]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hiq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Common_soapwort Common soapwort]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3HIQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hiq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Common_soapwort Common soapwort]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HIQ FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hio\|3hio]], [[3his\|3his]], [[3hit\|3hit]], [[3hiv\|3hiv]], [[3hiw\|3hiw]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3hio\|3hio]], [[3his\|3his]], [[3hit\|3hit]], [[3hiv\|3hiv]], [[3hiw\|3hiw]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3hiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hiq OCA], [~~http~~://pdbe.org/3hiq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3hiq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3hiq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3hiq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hiq OCA], [https://pdbe.org/3hiq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hiq RCSB], [https://www.ebi.ac.uk/pdbsum/3hiq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hiq ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 06:49, 7 August 2019

OCA — Wed, 07 Aug 2019 06:49:34 GMT

←Older revision		Revision as of 06:49, 7 August 2019
Line 1:		Line 1:

	==Crystal structure of Saporin-L1 mutant (Y73A) from Saponaria officinalis==		==Crystal structure of Saporin-L1 mutant (Y73A) from Saponaria officinalis==
-	<StructureSection load='3hiq' size='340' side='right' caption='[[3hiq]], [[Resolution\|resolution]] 2.19Å' scene=''>	+	<StructureSection load='3hiq' size='340' side='right'caption='[[3hiq]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3hiq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_soapwort Common soapwort]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HIQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hiq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_soapwort Common soapwort]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HIQ FirstGlance]. <br>
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/3hiq_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/3hiq_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</StructureSection>		</StructureSection>
	[[Category: Common soapwort]]		[[Category: Common soapwort]]
		+	[[Category: Large Structures]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]

OCA at 07:38, 1 November 2017

OCA — Wed, 01 Nov 2017 07:38:03 GMT

←Older revision		Revision as of 07:38, 1 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of Saporin-L1 mutant (Y73A) from Saponaria officinalis==		==Crystal structure of Saporin-L1 mutant (Y73A) from Saponaria officinalis==
	<StructureSection load='3hiq' size='340' side='right' caption='[[3hiq]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='3hiq' size='340' side='right' caption='[[3hiq]], [[Resolution\|resolution]] 2.19Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hio\|3hio]], [[3his\|3his]], [[3hit\|3hit]], [[3hiv\|3hiv]], [[3hiw\|3hiw]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hio\|3hio]], [[3his\|3his]], [[3hit\|3hit]], [[3hiv\|3hiv]], [[3hiw\|3hiw]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hiq OCA], [http://pdbe.org/3hiq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hiq RCSB], [http://www.ebi.ac.uk/pdbsum/3hiq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hiq OCA], [http://pdbe.org/3hiq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hiq RCSB], [http://www.ebi.ac.uk/pdbsum/3hiq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hiq ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 13:00, 9 February 2016

OCA — Tue, 09 Feb 2016 13:00:11 GMT

←Older revision		Revision as of 13:00, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3hiq' size='340' side='right' caption='[[3hiq]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='3hiq' size='340' side='right' caption='[[3hiq]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hiq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saponaria_officinalis Saponaria officinalis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HIQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hiq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_soapwort Common soapwort]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HIQ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hio\|3hio]], [[3his\|3his]], [[3hit\|3hit]], [[3hiv\|3hiv]], [[3hiw\|3hiw]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hio\|3hio]], [[3his\|3his]], [[3hit\|3hit]], [[3hiv\|3hiv]], [[3hiw\|3hiw]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hiq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hiq RCSB], [http://www.ebi.ac.uk/pdbsum/3hiq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hiq OCA], [http://pdbe.org/3hiq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hiq RCSB], [http://www.ebi.ac.uk/pdbsum/3hiq PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hiq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3hiq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saponaria officinalis~~]]	+	[[Category: Common soapwort]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]

OCA at 13:27, 6 November 2014

OCA — Thu, 06 Nov 2014 13:27:55 GMT

←Older revision		Revision as of 13:27, 6 November 2014
Line 1:		Line 1:
-	[[~~Image:~~3hiq.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of Saporin-L1 mutant (Y73A) from Saponaria officinalis==
		+	<StructureSection load='3hiq' size='340' side='right' caption='[[3hiq]], [[Resolution\|resolution]] 2.19Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3hiq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saponaria_officinalis Saponaria officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HIQ FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hio\|3hio]], [[3his\|3his]], [[3hit\|3hit]], [[3hiv\|3hiv]], [[3hiw\|3hiw]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hiq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hiq RCSB], [http://www.ebi.ac.uk/pdbsum/3hiq PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/3hiq_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ricin A-chain (RTA) and saporin-L1 (SAP) catalyze adenosine depurination of 28S rRNA to inhibit protein synthesis and cause cell death. We present the crystal structures of RTA and SAP in complex with transition state analogue inhibitors. These tight-binding inhibitors mimic the sarcin-ricin recognition loop of 28S rRNA and the dissociative ribocation transition state established for RTA catalysis. RTA and SAP share unique purine-binding geometry with quadruple pi-stacking interactions between adjacent adenine and guanine bases and 2 conserved tyrosines. An arginine at one end of the pi-stack provides cationic polarization and enhanced leaving group ability to the susceptible adenine. Common features of these ribosome-inactivating proteins include adenine leaving group activation, a remarkable lack of ribocation stabilization, and conserved glutamates as general bases for activation of the H(2)O nucleophile. Catalytic forces originate primarily from leaving group activation evident in both RTA and SAP in complex with transition state analogues.

-	~~{{STRUCTURE_3hiq\| PDB=3hiq \| SCENE= }}~~	+	Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins.,Ho MC, Sturm MB, Almo SC, Schramm VL Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20276-81. Epub 2009 Nov 17. PMID:19920175<ref>PMID:19920175</ref>

-	~~===Crystal structure~~ of ~~Saporin-L1 mutant (Y73A) from Saponaria officinalis===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19920175}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3hiq]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saponaria_officinalis Saponaria officinalis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019920175<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Saponaria officinalis]]		[[Category: Saponaria officinalis]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
-	[[Category: Almo, S C.]]	+	[[Category: Almo, S C]]
-	[[Category: Ho, M.]]	+	[[Category: Ho, M]]
-	[[Category: Schramm, V L.]]	+	[[Category: Schramm, V L]]
-	[[Category: Sturm, M B.]]	+	[[Category: Sturm, M B]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Plant defense]]		[[Category: Plant defense]]

OCA: Protected "3hiq" [edit=sysop:move=sysop]

OCA — Wed, 06 Mar 2013 14:55:07 GMT

Protected "3hiq" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:55, 6 March 2013

OCA at 14:55, 6 March 2013

OCA — Wed, 06 Mar 2013 14:55:04 GMT

←Older revision		Revision as of 14:55, 6 March 2013
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	[[Image:3hiq.png\|left\|200px]]		[[Image:3hiq.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3hiq\| PDB=3hiq \| SCENE= }}		{{STRUCTURE_3hiq\| PDB=3hiq \| SCENE= }}

	===Crystal structure of Saporin-L1 mutant (Y73A) from Saponaria officinalis===		===Crystal structure of Saporin-L1 mutant (Y73A) from Saponaria officinalis===

-
-	<!--
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	{{ABSTRACT_PUBMED_19920175}}		{{ABSTRACT_PUBMED_19920175}}

	==About this Structure==		==About this Structure==
-	~~3HIQ~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Saponaria_officinalis Saponaria officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA].	+	[[3hiq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saponaria_officinalis Saponaria officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19920175~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019920175</ref><references group="xtra"/>
	[[Category: Saponaria officinalis]]		[[Category: Saponaria officinalis]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
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	[[Category: Toxin]]		[[Category: Toxin]]
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OCA at 12:54, 28 January 2010

OCA — Thu, 28 Jan 2010 12:54:46 GMT

←Older revision		Revision as of 12:54, 28 January 2010
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	==About this Structure==		==About this Structure==
-	3HIQ is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Saponaria_officinalis Saponaria officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA].	+	3HIQ is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Saponaria_officinalis Saponaria officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HIQ OCA].

	==Reference==		==Reference==
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	[[Category: Toxin]]		[[Category: Toxin]]

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OCA at 09:31, 16 December 2009

OCA — Wed, 16 Dec 2009 09:31:31 GMT

←Older revision		Revision as of 09:31, 16 December 2009
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-	[[Image:3hiq.~~jpg~~\|left\|200px]]	+	[[Image:3hiq.png\|left\|200px]]

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