3hk6 - Revision history

OCA at 07:23, 6 September 2023

OCA — Wed, 06 Sep 2023 07:23:07 GMT

←Older revision		Revision as of 07:23, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3hk6' size='340' side='right'caption='[[3hk6]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='3hk6' size='340' side='right'caption='[[3hk6]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hk6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HK6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hk6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HK6 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1tq0\|1tq0]], [[3hk3\|3hk3]], [[3hki\|3hki]], [[3hkj\|3hkj]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">F2, Cf2 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10090 LK3 transgenic mice])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hk6 OCA], [https://pdbe.org/3hk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hk6 RCSB], [https://www.ebi.ac.uk/pdbsum/3hk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hk6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hk6 OCA], [https://pdbe.org/3hk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hk6 RCSB], [https://www.ebi.ac.uk/pdbsum/3hk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hk6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/THRB_MOUSE THRB_MOUSE]] Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity).	+	[https://www.uniprot.org/uniprot/THRB_MOUSE THRB_MOUSE] Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice]]~~	+	[[Category: Mus musculus]]
-	~~[[Category: Thrombin~~]]	+	[[Category: Bush-Pelc L]]
-	[[Category: Bush-Pelc, L]]	+	[[Category: Chen Z]]
-	[[Category: ~~Cera, E Di~~]]	+	[[Category: Di Cera E]]
-	[[Category: ~~Chen, Z~~]]	+	[[Category: Gandhi PS]]
-	[[Category: Gandhi~~, P S~~]]	+	[[Category: Page MJ]]
-	[[Category: Page~~, M J]]~~	+
-	~~[[Category: Acute phase]]~~	+
-	~~[[Category: Blood coagulation]]~~	+
-	~~[[Category: Calcium]]~~	+
-	~~[[Category: Cleavage on pair of basic residue]]~~	+
-	~~[[Category: Disulfide bond]]~~	+
-	~~[[Category: Gamma-carboxyglutamic acid]]~~	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Kringle]]~~	+
-	~~[[Category: Protease]]~~	+
-	~~[[Category: Serine protease]]~~	+
-	~~[[Category: Zymogen~~]]	+

OCA at 12:00, 13 October 2021

OCA — Wed, 13 Oct 2021 12:00:49 GMT

←Older revision		Revision as of 12:00, 13 October 2021
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	==Crystal structure of murine thrombin mutant W215A/E217A (two molecules in the asymmetric unit)==		==Crystal structure of murine thrombin mutant W215A/E217A (two molecules in the asymmetric unit)==
-	<StructureSection load='3hk6' size='340' side='right' caption='[[3hk6]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='3hk6' size='340' side='right'caption='[[3hk6]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hk6]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3HK6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hk6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HK6 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tq0\|1tq0]], [[3hk3\|3hk3]], [[3hki\|3hki]], [[3hkj\|3hkj]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1tq0\|1tq0]], [[3hk3\|3hk3]], [[3hki\|3hki]], [[3hkj\|3hkj]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F2, Cf2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F2, Cf2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hk6 OCA], [~~http~~://pdbe.org/3hk6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3hk6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3hk6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3hk6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hk6 OCA], [https://pdbe.org/3hk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hk6 RCSB], [https://www.ebi.ac.uk/pdbsum/3hk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hk6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/THRB_MOUSE THRB_MOUSE]] Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity).	+	[[https://www.uniprot.org/uniprot/THRB_MOUSE THRB_MOUSE]] Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
-	*[[Thrombin\|Thrombin]]	+	*[[Thrombin 3D Structures\|Thrombin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Thrombin]]		[[Category: Thrombin]]

OCA at 08:20, 12 December 2018

OCA — Wed, 12 Dec 2018 08:20:23 GMT

←Older revision		Revision as of 08:20, 12 December 2018
Line 1:		Line 1:
		+
	==Crystal structure of murine thrombin mutant W215A/E217A (two molecules in the asymmetric unit)==		==Crystal structure of murine thrombin mutant W215A/E217A (two molecules in the asymmetric unit)==
	<StructureSection load='3hk6' size='340' side='right' caption='[[3hk6]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='3hk6' size='340' side='right' caption='[[3hk6]], [[Resolution\|resolution]] 3.20Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F2, Cf2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F2, Cf2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hk6 OCA], [http://pdbe.org/3hk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hk6 RCSB], [http://www.ebi.ac.uk/pdbsum/3hk6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hk6 OCA], [http://pdbe.org/3hk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hk6 RCSB], [http://www.ebi.ac.uk/pdbsum/3hk6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hk6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/3hk6_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/3hk6_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 45:		Line 46:
	[[Category: Acute phase]]		[[Category: Acute phase]]
	[[Category: Blood coagulation]]		[[Category: Blood coagulation]]
		+	[[Category: Calcium]]
	[[Category: Cleavage on pair of basic residue]]		[[Category: Cleavage on pair of basic residue]]
	[[Category: Disulfide bond]]		[[Category: Disulfide bond]]

OCA at 00:33, 10 February 2016

OCA — Wed, 10 Feb 2016 00:33:05 GMT

←Older revision		Revision as of 00:33, 10 February 2016
Line 2:		Line 2:
	<StructureSection load='3hk6' size='340' side='right' caption='[[3hk6]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='3hk6' size='340' side='right' caption='[[3hk6]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hk6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HK6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hk6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HK6 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tq0\|1tq0]], [[3hk3\|3hk3]], [[3hki\|3hki]], [[3hkj\|3hkj]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tq0\|1tq0]], [[3hk3\|3hk3]], [[3hki\|3hki]], [[3hkj\|3hkj]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F2, Cf2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F2, Cf2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hk6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hk6 RCSB], [http://www.ebi.ac.uk/pdbsum/3hk6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hk6 OCA], [http://pdbe.org/3hk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hk6 RCSB], [http://www.ebi.ac.uk/pdbsum/3hk6 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hk6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3hk6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Thrombin]]		[[Category: Thrombin]]
	[[Category: Bush-Pelc, L]]		[[Category: Bush-Pelc, L]]

OCA at 10:33, 25 December 2014

OCA — Thu, 25 Dec 2014 10:33:10 GMT

←Older revision		Revision as of 10:33, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3hk6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HK6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hk6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HK6 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tq0\|1tq0]], [[3hk3\|3hk3]], [[3hki\|3hki]], [[3hkj\|3hkj]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tq0\|1tq0]], [[3hk3\|3hk3]], [[3hki\|3hki]], [[3hkj\|3hkj]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F2, Cf2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F2, Cf2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hk6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hk6 RCSB], [http://www.ebi.ac.uk/pdbsum/3hk6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hk6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hk6 RCSB], [http://www.ebi.ac.uk/pdbsum/3hk6 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/THRB_MOUSE THRB_MOUSE]] Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Thrombin]]		[[Category: Thrombin]]
-	[[Category: Bush-Pelc, L.]]	+	[[Category: Bush-Pelc, L]]
-	[[Category: Cera, E Di.]]	+	[[Category: Cera, E Di]]
-	[[Category: Chen, Z.]]	+	[[Category: Chen, Z]]
-	[[Category: Gandhi, P S.]]	+	[[Category: Gandhi, P S]]
-	[[Category: Page, M J.]]	+	[[Category: Page, M J]]
	[[Category: Acute phase]]		[[Category: Acute phase]]
	[[Category: Blood coagulation]]		[[Category: Blood coagulation]]

OCA at 12:38, 29 September 2014

OCA — Mon, 29 Sep 2014 12:38:41 GMT

←Older revision		Revision as of 12:38, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3hk6.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of murine thrombin mutant W215A/E217A (two molecules in the asymmetric unit)==
		+	<StructureSection load='3hk6' size='340' side='right' caption='[[3hk6]], [[Resolution\|resolution]] 3.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3hk6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HK6 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tq0\|1tq0]], [[3hk3\|3hk3]], [[3hki\|3hki]], [[3hkj\|3hkj]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F2, Cf2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hk6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hk6 RCSB], [http://www.ebi.ac.uk/pdbsum/3hk6 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/3hk6_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The thrombin mutant W215A/E217A (WE) is a potent anticoagulant both in vitro and in vivo. Previous x-ray structural studies have shown that WE assumes a partially collapsed conformation that is similar to the inactive E* form, which explains its drastically reduced activity toward substrate. Whether this collapsed conformation is genuine, rather than the result of crystal packing or the mutation introduced in the critical 215-217 beta-strand, and whether binding of thrombomodulin to exosite I can allosterically shift the E* form to the active E form to restore activity toward protein C are issues of considerable mechanistic importance to improve the design of an anticoagulant thrombin mutant for therapeutic applications. Here we present four crystal structures of WE in the human and murine forms that confirm the collapsed conformation reported previously under different experimental conditions and crystal packing. We also present structures of human and murine WE bound to exosite I with a fragment of the platelet receptor PAR1, which is unable to shift WE to the E form. These structural findings, along with kinetic and calorimetry data, indicate that WE is strongly stabilized in the E* form and explain why binding of ligands to exosite I has only a modest effect on the E-E equilibrium for this mutant. The E --> E transition requires the combined binding of thrombomodulin and protein C and restores activity of the mutant WE in the anticoagulant pathway.

-	~~{{STRUCTURE_3hk6\| PDB=3hk6 \| SCENE= }}~~	+	Mechanism of the anticoagulant activity of thrombin mutant W215A/E217A.,Gandhi PS, Page MJ, Chen Z, Bush-Pelc L, Di Cera E J Biol Chem. 2009 Sep 4;284(36):24098-105. Epub 2009 Jul 8. PMID:19586901<ref>PMID:19586901</ref>

-	~~===Crystal structure~~ of ~~murine thrombin mutant W215A/E217A (two molecules in~~ the ~~asymmetric unit)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19586901}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3hk6]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA].~~	+

	==See Also==		==See Also==
	*[[Thrombin\|Thrombin]]		*[[Thrombin\|Thrombin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019586901<~~/~~ref~~><~~ref group="xtra">PMID:015252033</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Thrombin]]		[[Category: Thrombin]]

OCA at 15:40, 20 October 2012

OCA — Sat, 20 Oct 2012 15:40:49 GMT

←Older revision		Revision as of 15:40, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3hk6]] is a 4 chain structure ~~of [[Thrombin]]~~ with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA].	+	[[3hk6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA].

	==See Also==		==See Also==

OCA at 19:08, 26 July 2012

OCA — Thu, 26 Jul 2012 19:08:40 GMT

←Older revision		Revision as of 19:08, 26 July 2012
Line 1:		Line 1:
	[[Image:3hk6.png\|left\|200px]]		[[Image:3hk6.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3hk6", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3hk6\| PDB=3hk6 \| SCENE= }}		{{STRUCTURE_3hk6\| PDB=3hk6 \| SCENE= }}

	===Crystal structure of murine thrombin mutant W215A/E217A (two molecules in the asymmetric unit)===		===Crystal structure of murine thrombin mutant W215A/E217A (two molecules in the asymmetric unit)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19586901}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19586901 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19586901}}		{{ABSTRACT_PUBMED_19586901}}

	==About this Structure==		==About this Structure==
-	[[3hk6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA].	+	[[3hk6]] is a 4 chain structure of [[Thrombin]] with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA].
		+
		+	==See Also==
		+	*[[Thrombin\|Thrombin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19586901~~</ref><ref group="xtra">PMID:~~15252033~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019586901</ref><ref group="xtra">PMID:015252033</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Thrombin]]		[[Category: Thrombin]]
Line 32:		Line 24:
	[[Category: Acute phase]]		[[Category: Acute phase]]
	[[Category: Blood coagulation]]		[[Category: Blood coagulation]]
-	[[Category: Calcium]]
	[[Category: Cleavage on pair of basic residue]]		[[Category: Cleavage on pair of basic residue]]
	[[Category: Disulfide bond]]		[[Category: Disulfide bond]]

OCA: Protected "3hk6" [edit=sysop:move=sysop]

OCA — Mon, 14 Mar 2011 22:50:11 GMT

Protected "3hk6" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:50, 14 March 2011

OCA at 22:50, 14 March 2011

OCA — Mon, 14 Mar 2011 22:50:09 GMT

←Older revision		Revision as of 22:50, 14 March 2011
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3hk6.png\|left\|200px]]		[[Image:3hk6.png\|left\|200px]]

Line 20:		Line 19:

	==About this Structure==		==About this Structure==
-	~~3HK6~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA].	+	[[3hk6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HK6 OCA].

	==Reference==		==Reference==
Line 43:		Line 42:
	[[Category: Serine protease]]		[[Category: Serine protease]]
	[[Category: Zymogen]]		[[Category: Zymogen]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 16 08:30:16 2009''