3hn1 - Revision history

OCA at 07:24, 6 September 2023

OCA — Wed, 06 Sep 2023 07:24:38 GMT

←Older revision		Revision as of 07:24, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3hn1' size='340' side='right'caption='[[3hn1]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3hn1' size='340' side='right'caption='[[3hn1]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hn1]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_tetani"_flugge_1886 "bacillus~~ tetani~~" flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3HN1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hn1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HN1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GLA:ALPHA+D~~-~~GALACTOSE'>GLA</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~SIA~~:O-~~SIALIC+ACID~~'>~~SIA~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat">[[1fv2\|1fv2]]~~, ~~[[3hmy\|3hmy]]~~<~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hn1 OCA], [https://pdbe.org/3hn1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hn1 RCSB], [https://www.ebi.ac.uk/pdbsum/3hn1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hn1 ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3hn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hn1 OCA], [~~http~~://pdbe.org/3hn1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3hn1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3hn1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3hn1 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TETX_CLOTE TETX_CLOTE]] Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-\|-Phe-77' bond of synaptobrevin-2.	+	[https://www.uniprot.org/uniprot/TETX_CLOTE TETX_CLOTE] Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-\|-Phe-77' bond of synaptobrevin-2.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ tetani ~~flugge 1886~~]]	+	[[Category: Clostridium tetani]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Tentoxilysin]]~~	+	[[Category: Baldwin MR]]
-	[[Category: Baldwin~~, M R~~]]	+	[[Category: Barbieri JT]]
-	[[Category: Barbieri~~, J T~~]]	+	[[Category: Chen C]]
-	[[Category: Chen, C]]	+	[[Category: Fu Z]]
-	[[Category: Fu, Z]]	+	[[Category: Kim J-JP]]
-	[[Category: Kim, J ~~J.P]]~~	+
-	~~[[Category: Disulfide bond]]~~	+
-	~~[[Category: Ganglioside gt2]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Lactose]]~~	+
-	~~[[Category: Metal~~-~~binding]]~~	+
-	~~[[Category: Metalloprotease]]~~	+
-	~~[[Category: Neurotoxin]]~~	+
-	~~[[Category: Protease]]~~	+
-	~~[[Category: Receptor binding pocket]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 10:49, 16 September 2020

OCA — Wed, 16 Sep 2020 10:49:36 GMT

←Older revision		Revision as of 10:49, 16 September 2020
Line 1:		Line 1:

	==Crystal structure of HCR/T complexed with GT2 and lactose==		==Crystal structure of HCR/T complexed with GT2 and lactose==
-	<StructureSection load='3hn1' size='340' side='right' caption='[[3hn1]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3hn1' size='340' side='right'caption='[[3hn1]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hn1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tetani"_flugge_1886 "bacillus tetani" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3HN1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hn1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tetani"_flugge_1886 "bacillus tetani" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3HN1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=~~SIA~~:~~O-SIALIC~~+~~ACID~~'>~~SIA~~</scene>, <scene name='pdbligand=~~SO4~~:~~SULFATE~~+~~ION~~'>~~SO4~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fv2\|1fv2]], [[3hmy\|3hmy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fv2\|1fv2]], [[3hmy\|3hmy]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3hn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hn1 OCA], [http://pdbe.org/3hn1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hn1 RCSB], [http://www.ebi.ac.uk/pdbsum/3hn1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hn1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3hn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hn1 OCA], [http://pdbe.org/3hn1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hn1 RCSB], [http://www.ebi.ac.uk/pdbsum/3hn1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hn1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 38:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus tetani flugge 1886]]		[[Category: Bacillus tetani flugge 1886]]
		+	[[Category: Large Structures]]
	[[Category: Tentoxilysin]]		[[Category: Tentoxilysin]]
	[[Category: Baldwin, M R]]		[[Category: Baldwin, M R]]

OCA at 08:10, 12 December 2018

OCA — Wed, 12 Dec 2018 08:10:09 GMT

←Older revision		Revision as of 08:10, 12 December 2018
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3hn1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tetani"_flugge_1886 "bacillus tetani" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HN1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hn1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tetani"_flugge_1886 "bacillus tetani" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HN1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=~~SO4~~:~~SULFATE~~+~~ION~~'>~~SO4~~</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~SIA~~:~~O-SIALIC~~+~~ACID~~'>~~SIA~~</scene></td></tr>	+
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fv2\|1fv2]], [[3hmy\|3hmy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fv2\|1fv2]], [[3hmy\|3hmy]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] </span></td></tr>
Line 16:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hn/3hn1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hn/3hn1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 10:04, 5 August 2016

OCA — Fri, 05 Aug 2016 10:04:28 GMT

←Older revision		Revision as of 10:04, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of HCR/T complexed with GT2 and lactose==		==Crystal structure of HCR/T complexed with GT2 and lactose==
	<StructureSection load='3hn1' size='340' side='right' caption='[[3hn1]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3hn1' size='340' side='right' caption='[[3hn1]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hn1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_tetani Clostridium~~ tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HN1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hn1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tetani"_flugge_1886 "bacillus tetani" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HN1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fv2\|1fv2]], [[3hmy\|3hmy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fv2\|1fv2]], [[3hmy\|3hmy]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hn1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hn1 RCSB], [http://www.ebi.ac.uk/pdbsum/3hn1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hn1 OCA], [http://pdbe.org/3hn1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hn1 RCSB], [http://www.ebi.ac.uk/pdbsum/3hn1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hn1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hn1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 30:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3hn1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clostridium~~ tetani]]	+	[[Category: Bacillus tetani flugge 1886]]
	[[Category: Tentoxilysin]]		[[Category: Tentoxilysin]]
	[[Category: Baldwin, M R]]		[[Category: Baldwin, M R]]

OCA at 17:54, 25 December 2014

OCA — Thu, 25 Dec 2014 17:54:49 GMT

←Older revision		Revision as of 17:54, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hn1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hn1 RCSB], [http://www.ebi.ac.uk/pdbsum/3hn1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hn1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hn1 RCSB], [http://www.ebi.ac.uk/pdbsum/3hn1 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TETX_CLOTE TETX_CLOTE]] Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-\|-Phe-77' bond of synaptobrevin-2.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:24, 8 December 2014

OCA — Mon, 08 Dec 2014 09:24:42 GMT

←Older revision		Revision as of 09:24, 8 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3hn1.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of HCR/T complexed with GT2 and lactose==
		+	<StructureSection load='3hn1' size='340' side='right' caption='[[3hn1]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3hn1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HN1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fv2\|1fv2]], [[3hmy\|3hmy]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hn1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hn1 RCSB], [http://www.ebi.ac.uk/pdbsum/3hn1 PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hn/3hn1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tetanus neurotoxin (TeNT) is an exotoxin produced by Clostridium tetani that causes paralytic death to hundreds of thousands of humans annually. TeNT cleaves vesicle-associated membrane protein-2, which inhibits neurotransmitter release in the central nervous system to elicit spastic paralysis, but the molecular basis for TeNT entry into neurons remains unclear. TeNT is a approximately 150-kDa protein that has AB structure-function properties; the A domain is a zinc metalloprotease, and the B domain encodes a translocation domain and C-terminal receptor-binding domain (HCR/T). Earlier studies showed that HCR/T bound gangliosides via two carbohydrate-binding sites, termed the lactose-binding site (the "W" pocket) and the sialic acid-binding site (the "R" pocket). Here we report that TeNT high affinity binding to neurons is mediated solely by gangliosides. Glycan array and solid phase binding analyses identified gangliosides that bound exclusively to either the W pocket or the R pocket of TeNT; GM1a bound to the W pocket, and GD3 bound to the R pocket. Using these gangliosides and mutated forms of HCR/T that lacked one or both carbohydrate-binding pocket, gangliosides binding to both of the W and R pockets were shown to be necessary for high affinity binding to neuronal and non-neuronal cells. The crystal structure of a ternary complex of HCR/T with sugar components of two gangliosides bound to the W and R supported the binding of gangliosides to both carbohydrate pockets. These data show that gangliosides are functional dual receptors for TeNT.

-	~~{{STRUCTURE_3hn1\| PDB=3hn1 \| SCENE= }}~~	+	Gangliosides as high affinity receptors for tetanus neurotoxin.,Chen C, Fu Z, Kim JJ, Barbieri JT, Baldwin MR J Biol Chem. 2009 Sep 25;284(39):26569-77. Epub 2009 Jul 14. PMID:19602728<ref>PMID:19602728</ref>

-	~~===Crystal structure~~ of ~~HCR~~/~~T complexed with GT2 and lactose===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_19602728}}~~	+	==See Also==
-		+	*[[Tetanus toxin\|Tetanus toxin]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~3hn1~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:019602728<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Clostridium tetani]]		[[Category: Clostridium tetani]]
	[[Category: Tentoxilysin]]		[[Category: Tentoxilysin]]
-	[[Category: Baldwin, M R.]]	+	[[Category: Baldwin, M R]]
-	[[Category: Barbieri, J T.]]	+	[[Category: Barbieri, J T]]
-	[[Category: Chen, C.]]	+	[[Category: Chen, C]]
-	[[Category: Fu, Z.]]	+	[[Category: Fu, Z]]
-	[[Category: Kim, J J.P.]]	+	[[Category: Kim, J J.P]]
	[[Category: Disulfide bond]]		[[Category: Disulfide bond]]
	[[Category: Ganglioside gt2]]		[[Category: Ganglioside gt2]]

OCA: Protected "3hn1" [edit=sysop:move=sysop]

OCA — Wed, 06 Mar 2013 14:47:53 GMT

Protected "3hn1" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:47, 6 March 2013

OCA at 14:47, 6 March 2013

OCA — Wed, 06 Mar 2013 14:47:51 GMT

←Older revision		Revision as of 14:47, 6 March 2013
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-	{{Seed}}
	[[Image:3hn1.png\|left\|200px]]		[[Image:3hn1.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3hn1", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3hn1\| PDB=3hn1 \| SCENE= }}		{{STRUCTURE_3hn1\| PDB=3hn1 \| SCENE= }}

	===Crystal structure of HCR/T complexed with GT2 and lactose===		===Crystal structure of HCR/T complexed with GT2 and lactose===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19602728}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19602728 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19602728}}		{{ABSTRACT_PUBMED_19602728}}

	==About this Structure==		==About this Structure==
-	~~3HN1~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA].	+	[[3hn1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19602728~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019602728</ref><references group="xtra"/>
	[[Category: Clostridium tetani]]		[[Category: Clostridium tetani]]
	[[Category: Tentoxilysin]]		[[Category: Tentoxilysin]]
Line 41:		Line 29:
	[[Category: Receptor binding pocket]]		[[Category: Receptor binding pocket]]
	[[Category: Toxin]]		[[Category: Toxin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Aug 12 00:06:04 2010''

OCA at 19:58, 11 August 2010

OCA — Wed, 11 Aug 2010 19:58:47 GMT

←Older revision		Revision as of 19:58, 11 August 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3hn1.~~jpg~~\|left\|200px]]	+	[[Image:3hn1.png\|left\|200px]]

	<!--		<!--
Line 12:		Line 12:
	===Crystal structure of HCR/T complexed with GT2 and lactose===		===Crystal structure of HCR/T complexed with GT2 and lactose===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19602728}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19602728 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19602728}}

	==About this Structure==		==About this Structure==
-	3HN1 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA].	+	3HN1 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19602728</ref><references group="xtra"/>
	[[Category: Clostridium tetani]]		[[Category: Clostridium tetani]]
	[[Category: Tentoxilysin]]		[[Category: Tentoxilysin]]
Line 29:		Line 38:
	[[Category: Metalloprotease]]		[[Category: Metalloprotease]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
-	[[Category: Plasmid]]
	[[Category: Protease]]		[[Category: Protease]]
	[[Category: Receptor binding pocket]]		[[Category: Receptor binding pocket]]
	[[Category: Toxin]]		[[Category: Toxin]]
-	[[Category: Zinc]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 15 13~~:53:04 ~~2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Aug 12 00:06:04 2010''

OCA at 10:53, 15 July 2009

OCA — Wed, 15 Jul 2009 10:53:06 GMT

←Older revision		Revision as of 10:53, 15 July 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3hn1.jpg\|left\|200px]]

-	The ~~entry 3hn1~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3hn1", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3hn1\| PDB=3hn1 \| SCENE= }}

-	~~Authors: Chen, C., Fu, Z., Kim, J.-J.P., Barbieri, J.~~T~~., Baldwin, M.R.~~	+	===Crystal structure of HCR/T complexed with GT2 and lactose===

-	Description: Crystal structure of HCR/T complexed with GT2 and lactose

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jun 10 18~~:00:07 2009''	+	==About this Structure==
		+	3HN1 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN1 OCA].
		+	[[Category: Clostridium tetani]]
		+	[[Category: Tentoxilysin]]
		+	[[Category: Baldwin, M R.]]
		+	[[Category: Barbieri, J T.]]
		+	[[Category: Chen, C.]]
		+	[[Category: Fu, Z.]]
		+	[[Category: Kim, J J.P.]]
		+	[[Category: Disulfide bond]]
		+	[[Category: Ganglioside gt2]]
		+	[[Category: Hydrolase]]
		+	[[Category: Lactose]]
		+	[[Category: Metal-binding]]
		+	[[Category: Metalloprotease]]
		+	[[Category: Neurotoxin]]
		+	[[Category: Plasmid]]
		+	[[Category: Protease]]
		+	[[Category: Receptor binding pocket]]
		+	[[Category: Toxin]]
		+	[[Category: Zinc]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 15 13:53:04 2009''