3hsj - Revision history

OCA at 13:09, 30 August 2023

OCA — Wed, 30 Aug 2023 13:09:28 GMT

←Older revision		Revision as of 13:09, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='3hsj' size='340' side='right'caption='[[3hsj]], [[Resolution\|resolution]] 1.18Å' scene=''>		<StructureSection load='3hsj' size='340' side='right'caption='[[3hsj]], [[Resolution\|resolution]] 1.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hsj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HSJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hsj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HSJ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.18Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1hka\|1hka]], [[3hsd\|3hsd]], [[3hsg\|3hsg]], [[3hsz\|3hsz]], [[3ht0\|3ht0]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>b0142, foIK, folK, JW0138 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~83333 ECOLI])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[https://en.wikipedia.org/wiki/2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_diphosphokinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.3 2.7.6.3] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hsj OCA], [https://pdbe.org/3hsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hsj RCSB], [https://www.ebi.ac.uk/pdbsum/3hsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hsj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hsj OCA], [https://pdbe.org/3hsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hsj RCSB], [https://www.ebi.ac.uk/pdbsum/3hsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hsj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/HPPK_ECOLI HPPK_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hsj ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hsj ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: Folate cofactors are essential for life. Mammals derive folates from their diet, whereas most microorganisms must synthesize folates de novo. Enzymes of the folate pathway therefore provide ideal targets for the development of antimicrobial agents. 6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the transfer of pyrophosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in the folate biosynthetic pathway. RESULTS: The crystal structure of HPPK from Escherichia coli has been determined at 1.5 A resolution with a crystallographic R factor of 0.182. The HPPK molecule has a novel three-layered alpha beta alpha fold that creates a valley approximately 26 A long, 10 A wide and 10 A deep. The active center of HPPK is located in the valley and the substrate-binding sites have been identified with the aid of NMR spectroscopy. The HP-binding site is located at one end of the valley, near Asn55, and is sandwiched between two aromatic sidechains. The ATP-binding site is located at the other end of the valley. The adenine base of ATP is positioned near Leu111 and the ribose and the triphosphate extend across and reach the vicinity of HP. CONCLUSIONS: The HPPK structure provides a framework to elucidate structure/function relationships of the enzyme and to analyze mechanisms of pyrophosphoryl transfer. Furthermore, this work may prove useful in the structure-based design of new antimicrobial agents.
-
-	Crystal structure of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, a potential target for the development of novel antimicrobial agents.,Xiao B, Shi G, Chen X, Yan H, Ji X Structure. 1999 May;7(5):489-96. PMID:10378268<ref>PMID:10378268</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3hsj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[HPPK 3D structures\|HPPK 3D structures]]		*[[HPPK 3D structures\|HPPK 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 2-~~amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Ecoli~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Blaszczyk, J]]	+	[[Category: Blaszczyk J]]
-	[[Category: Ji, X]]	+	[[Category: Ji X]]
-	[[Category: Li, Y]]	+	[[Category: Li Y]]
-	[[Category: Yan, H]]	+	[[Category: Yan H]]
-	~~[[Category: Alpha beta]]~~	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Folate biosynthesis]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 12:03, 13 October 2021

OCA — Wed, 13 Oct 2021 12:03:24 GMT

←Older revision		Revision as of 12:03, 13 October 2021
Line 1:		Line 1:

	==Crystal structure of E. coli HPPK(N55A)==		==Crystal structure of E. coli HPPK(N55A)==
-	<StructureSection load='3hsj' size='340' side='right' caption='[[3hsj]], [[Resolution\|resolution]] 1.18Å' scene=''>	+	<StructureSection load='3hsj' size='340' side='right'caption='[[3hsj]], [[Resolution\|resolution]] 1.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hsj]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3HSJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hsj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HSJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hka\|1hka]], [[3hsd\|3hsd]], [[3hsg\|3hsg]], [[3hsz\|3hsz]], [[3ht0\|3ht0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hka\|1hka]], [[3hsd\|3hsd]], [[3hsg\|3hsg]], [[3hsz\|3hsz]], [[3ht0\|3ht0]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0142, foIK, folK, JW0138 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0142, foIK, folK, JW0138 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_diphosphokinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.3 2.7.6.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_diphosphokinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.3 2.7.6.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3hsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hsj OCA], [~~http~~://pdbe.org/3hsj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3hsj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3hsj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3hsj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hsj OCA], [https://pdbe.org/3hsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hsj RCSB], [https://www.ebi.ac.uk/pdbsum/3hsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hsj ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hs/3hsj_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hs/3hsj_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 3hsj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3hsj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[HPPK 3D structures\|HPPK 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	[[Category: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase]]		[[Category: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Blaszczyk, J]]		[[Category: Blaszczyk, J]]
	[[Category: Ji, X]]		[[Category: Ji, X]]

OCA at 07:46, 1 November 2017

OCA — Wed, 01 Nov 2017 07:46:26 GMT

←Older revision		Revision as of 07:46, 1 November 2017
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 3hsj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3hsj" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[6-hydroxymethyl-7%2C8-dihydropterin pyrophosphokinase\|6-hydroxymethyl-7%2C8-dihydropterin pyrophosphokinase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:36, 9 December 2016

OCA — Fri, 09 Dec 2016 20:36:31 GMT

←Older revision		Revision as of 20:36, 9 December 2016
Line 1:		Line 1:
		+
	==Crystal structure of E. coli HPPK(N55A)==		==Crystal structure of E. coli HPPK(N55A)==
	<StructureSection load='3hsj' size='340' side='right' caption='[[3hsj]], [[Resolution\|resolution]] 1.18Å' scene=''>		<StructureSection load='3hsj' size='340' side='right' caption='[[3hsj]], [[Resolution\|resolution]] 1.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hsj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HSJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hsj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HSJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hka\|1hka]], [[3hsd\|3hsd]], [[3hsg\|3hsg]], [[3hsz\|3hsz]], [[3ht0\|3ht0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hka\|1hka]], [[3hsd\|3hsd]], [[3hsg\|3hsg]], [[3hsz\|3hsz]], [[3ht0\|3ht0]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0142, foIK, folK, JW0138 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0142, foIK, folK, JW0138 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_diphosphokinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.3 2.7.6.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_diphosphokinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.3 2.7.6.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hsj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hsj RCSB], [http://www.ebi.ac.uk/pdbsum/3hsj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hsj OCA], [http://pdbe.org/3hsj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hsj RCSB], [http://www.ebi.ac.uk/pdbsum/3hsj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hsj ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hsj ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3hsj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase]]		[[Category: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase]]
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Ecoli]]
	[[Category: Blaszczyk, J]]		[[Category: Blaszczyk, J]]
	[[Category: Ji, X]]		[[Category: Ji, X]]

OCA at 09:12, 8 December 2014

OCA — Mon, 08 Dec 2014 09:12:23 GMT

←Older revision		Revision as of 09:12, 8 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3hsj.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of E. coli HPPK(N55A)==
		+	<StructureSection load='3hsj' size='340' side='right' caption='[[3hsj]], [[Resolution\|resolution]] 1.18Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3hsj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HSJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hka\|1hka]], [[3hsd\|3hsd]], [[3hsg\|3hsg]], [[3hsz\|3hsz]], [[3ht0\|3ht0]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0142, foIK, folK, JW0138 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_diphosphokinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.3 2.7.6.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hsj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hsj RCSB], [http://www.ebi.ac.uk/pdbsum/3hsj PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hs/3hsj_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Folate cofactors are essential for life. Mammals derive folates from their diet, whereas most microorganisms must synthesize folates de novo. Enzymes of the folate pathway therefore provide ideal targets for the development of antimicrobial agents. 6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the transfer of pyrophosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in the folate biosynthetic pathway. RESULTS: The crystal structure of HPPK from Escherichia coli has been determined at 1.5 A resolution with a crystallographic R factor of 0.182. The HPPK molecule has a novel three-layered alpha beta alpha fold that creates a valley approximately 26 A long, 10 A wide and 10 A deep. The active center of HPPK is located in the valley and the substrate-binding sites have been identified with the aid of NMR spectroscopy. The HP-binding site is located at one end of the valley, near Asn55, and is sandwiched between two aromatic sidechains. The ATP-binding site is located at the other end of the valley. The adenine base of ATP is positioned near Leu111 and the ribose and the triphosphate extend across and reach the vicinity of HP. CONCLUSIONS: The HPPK structure provides a framework to elucidate structure/function relationships of the enzyme and to analyze mechanisms of pyrophosphoryl transfer. Furthermore, this work may prove useful in the structure-based design of new antimicrobial agents.

-	~~{{STRUCTURE_3hsj\| PDB=3hsj \| SCENE= }}~~	+	Crystal structure of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, a potential target for the development of novel antimicrobial agents.,Xiao B, Shi G, Chen X, Yan H, Ji X Structure. 1999 May;7(5):489-96. PMID:10378268<ref>PMID:10378268</ref>

-	~~===Crystal structure~~ of E. ~~coli HPPK(N55A)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_10378268}}~~	+	==See Also==
-		+	*[[6-hydroxymethyl-7%2C8-dihydropterin pyrophosphokinase\|6-hydroxymethyl-7%2C8-dihydropterin pyrophosphokinase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~3hsj~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSJ OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:010378268<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase]]		[[Category: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Blaszczyk, J.]]	+	[[Category: Blaszczyk, J]]
-	[[Category: Ji, X.]]	+	[[Category: Ji, X]]
-	[[Category: Li, Y.]]	+	[[Category: Li, Y]]
-	[[Category: Yan, H.]]	+	[[Category: Yan, H]]
	[[Category: Alpha beta]]		[[Category: Alpha beta]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]

OCA: Protected "3hsj" [edit=sysop:move=sysop]

OCA — Wed, 06 Mar 2013 15:06:37 GMT

Protected "3hsj" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:06, 6 March 2013

OCA at 15:06, 6 March 2013

OCA — Wed, 06 Mar 2013 15:06:32 GMT

←Older revision		Revision as of 15:06, 6 March 2013
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3hsj.png\|left\|200px]]
-	[[Image:3hsj.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3hsj", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3hsj\| PDB=3hsj \| SCENE= }}		{{STRUCTURE_3hsj\| PDB=3hsj \| SCENE= }}

	===Crystal structure of E. coli HPPK(N55A)===		===Crystal structure of E. coli HPPK(N55A)===

		+	{{ABSTRACT_PUBMED_10378268}}

	==About this Structure==		==About this Structure==
-	~~3HSJ~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSJ OCA].	+	[[3hsj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSJ OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10378268~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010378268</ref><references group="xtra"/>
	[[Category: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase]]		[[Category: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
Line 30:		Line 24:
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 23 08:48:44 2010''

OCA at 04:43, 23 June 2010

OCA — Wed, 23 Jun 2010 04:43:27 GMT

←Older revision		Revision as of 04:43, 23 June 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3hsj.jpg\|left\|200px]]

-	The ~~entry 3hsj~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3hsj", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3hsj\| PDB=3hsj \| SCENE= }}

-	~~Authors: Blaszczyk, J., Li, Y., Yan, H., Ji, X~~.	+	===Crystal structure of E. coli HPPK(N55A)===

-	Description: Crystal structure of E. coli HPPK(N55A)

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Jun 25 08:11:~~46 2009~~''	+	==About this Structure==
		+	3HSJ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HSJ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:10378268</ref><references group="xtra"/>
		+	[[Category: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase]]
		+	[[Category: Escherichia coli]]
		+	[[Category: Blaszczyk, J.]]
		+	[[Category: Ji, X.]]
		+	[[Category: Li, Y.]]
		+	[[Category: Yan, H.]]
		+	[[Category: Alpha beta]]
		+	[[Category: Atp-binding]]
		+	[[Category: Folate biosynthesis]]
		+	[[Category: Kinase]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Transferase]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 23 08:48:44 2010''

OCA at 05:11, 25 June 2009

OCA — Thu, 25 Jun 2009 05:11:46 GMT

←Older revision		Revision as of 05:11, 25 June 2009
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3hsj is ON HOLD	+	The entry 3hsj is ON HOLD until Paper Publication

-	Authors: Blaszczyk, ~~Jaroslaw~~, Li, ~~Yue~~, Yan, ~~Honggao~~, Ji, ~~Xinhua~~	+	Authors: Blaszczyk, J., Li, Y., Yan, H., Ji, X.

	Description: Crystal structure of E. coli HPPK(N55A)		Description: Crystal structure of E. coli HPPK(N55A)

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ Jun ~~17 09:49~~:11 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:11:46 2009''

OCA: New page: '''Unreleased structure''' The entry 3hsj is ON HOLD Authors: Blaszczyk, Jaroslaw, Li, Yue, Yan, Honggao, Ji, Xinhua Description: Crystal structure of E. coli HPPK(N55A) ''Page seeded...

OCA — Wed, 17 Jun 2009 06:49:11 GMT

New page: '''Unreleased structure''' The entry 3hsj is ON HOLD Authors: Blaszczyk, Jaroslaw, Li, Yue, Yan, Honggao, Ji, Xinhua Description: Crystal structure of E. coli HPPK(N55A) ''Page seeded...

New page

'''Unreleased structure'''

The entry 3hsj is ON HOLD

Authors: Blaszczyk, Jaroslaw, Li, Yue, Yan, Honggao, Ji, Xinhua

Description: Crystal structure of E. coli HPPK(N55A)

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 17 09:49:11 2009''