3hvd - Revision history

OCA at 14:05, 13 March 2024

OCA — Wed, 13 Mar 2024 14:05:25 GMT

←Older revision		Revision as of 14:05, 13 March 2024
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hvd ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hvd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The assembly of bacterial toxins and virulence factors is critical to their function, but the regulation of assembly during infection has not been studied. We begin to address this question using anthrax toxin as a model. The protective antigen (PA) component of the toxin assembles into ring-shaped homooligomers that bind the two other enzyme components of the toxin, lethal factor (LF) and edema factor (EF), to form toxic complexes. To disrupt the host, these toxic complexes are endocytosed, such that the PA oligomer forms a membrane-spanning channel that LF and EF translocate through to enter the cytosol. Using single-channel electrophysiology, we show that PA channels contain two populations of conductance states, which correspond to two different PA pre-channel oligomers observed by electron microscopy-the well-described heptamer and a novel octamer. Mass spectrometry demonstrates that the PA octamer binds four LFs, and assembly routes leading to the octamer are populated with even-numbered, dimeric and tetrameric, PA intermediates. Both heptameric and octameric PA complexes can translocate LF and EF with similar rates and efficiencies. Here, we report a 3.2-A crystal structure of the PA octamer. The octamer comprises approximately 20-30% of the oligomers on cells, but outside of the cell, the octamer is more stable than the heptamer under physiological pH. Thus, the PA octamer is a physiological, stable, and active assembly state capable of forming lethal toxins that may withstand the hostile conditions encountered in the bloodstream. This assembly mechanism may provide a novel means to control cytotoxicity.
-
-	The protective antigen component of anthrax toxin forms functional octameric complexes.,Kintzer AF, Thoren KL, Sterling HJ, Dong KC, Feld GK, Tang II, Zhang TT, Williams ER, Berger JM, Krantz BA J Mol Biol. 2009 Sep 25;392(3):614-29. Epub 2009 Jul 20. PMID:19627991<ref>PMID:19627991</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3hvd" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:32, 6 September 2023

OCA — Wed, 06 Sep 2023 07:32:01 GMT

←Older revision		Revision as of 07:32, 6 September 2023
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	<StructureSection load='3hvd' size='340' side='right'caption='[[3hvd]], [[Resolution\|resolution]] 3.21Å' scene=''>		<StructureSection load='3hvd' size='340' side='right'caption='[[3hvd]], [[Resolution\|resolution]] 3.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hvd]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var.~~ anthracis~~" (cohn 1872) smith et al. 1946~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HVD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hvd]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HVD FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.209Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>pa, PAG ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1392~~ "~~Bacillus cereus var. anthracis~~" ~~(Cohn 1872) Smith et al. 1946]), pa ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hvd OCA], [https://pdbe.org/3hvd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hvd RCSB], [https://www.ebi.ac.uk/pdbsum/3hvd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hvd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hvd OCA], [https://pdbe.org/3hvd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hvd RCSB], [https://www.ebi.ac.uk/pdbsum/3hvd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hvd ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q08G54_BACAN Q08G54_BACAN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Large Structures]]
-	[[Category: Berger, J M]]
-	[[Category: Dong, K C]]
-	[[Category: Kintzer, A F]]
-	[[Category: Krantz, B A]]
-	[[Category: Anthrax protective antigen]]
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
-	[[Category: ~~Octamer~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Toxin~~]]	+	[[Category: Berger JM]]
-	[[Category: ~~Transport protein~~]]	+	[[Category: Dong KC]]
-	[[Category: ~~Xray-crystallography~~]]	+	[[Category: Kintzer AF]]
		+	[[Category: Krantz BA]]

OCA at 12:04, 13 October 2021

OCA — Wed, 13 Oct 2021 12:04:38 GMT

←Older revision		Revision as of 12:04, 13 October 2021
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	==The Protective Antigen Component of Anthrax Toxin Forms Functional Octameric Complexes==		==The Protective Antigen Component of Anthrax Toxin Forms Functional Octameric Complexes==
-	<StructureSection load='3hvd' size='340' side='right' caption='[[3hvd]], [[Resolution\|resolution]] 3.21Å' scene=''>	+	<StructureSection load='3hvd' size='340' side='right'caption='[[3hvd]], [[Resolution\|resolution]] 3.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hvd]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3HVD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hvd]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HVD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pa, PAG ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946]), pa ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pa, PAG ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946]), pa ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3hvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hvd OCA], [~~http~~://pdbe.org/3hvd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3hvd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3hvd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3hvd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hvd OCA], [https://pdbe.org/3hvd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hvd RCSB], [https://www.ebi.ac.uk/pdbsum/3hvd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hvd ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hv/3hvd_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hv/3hvd_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Berger, J M]]		[[Category: Berger, J M]]
	[[Category: Dong, K C]]		[[Category: Dong, K C]]

OCA at 00:45, 16 November 2017

OCA — Thu, 16 Nov 2017 00:45:34 GMT

←Older revision		Revision as of 00:45, 16 November 2017
Line 1:		Line 1:
		+
	==The Protective Antigen Component of Anthrax Toxin Forms Functional Octameric Complexes==		==The Protective Antigen Component of Anthrax Toxin Forms Functional Octameric Complexes==
	<StructureSection load='3hvd' size='340' side='right' caption='[[3hvd]], [[Resolution\|resolution]] 3.21Å' scene=''>		<StructureSection load='3hvd' size='340' side='right' caption='[[3hvd]], [[Resolution\|resolution]] 3.21Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pa, PAG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946]), pa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pa, PAG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946]), pa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hvd OCA], [http://pdbe.org/3hvd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hvd RCSB], [http://www.ebi.ac.uk/pdbsum/3hvd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hvd OCA], [http://pdbe.org/3hvd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hvd RCSB], [http://www.ebi.ac.uk/pdbsum/3hvd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hvd ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</div>		</div>
	<div class="pdbe-citations 3hvd" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3hvd" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Anthrax protective antigen\|Anthrax protective antigen]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:59, 9 February 2016

OCA — Tue, 09 Feb 2016 00:59:57 GMT

←Older revision		Revision as of 00:59, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3hvd' size='340' side='right' caption='[[3hvd]], [[Resolution\|resolution]] 3.21Å' scene=''>		<StructureSection load='3hvd' size='340' side='right' caption='[[3hvd]], [[Resolution\|resolution]] 3.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hvd]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_anthracis Bacillus~~ anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HVD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hvd]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HVD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pa, PAG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis]), pa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pa, PAG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946]), pa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hvd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hvd RCSB], [http://www.ebi.ac.uk/pdbsum/3hvd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hvd OCA], [http://pdbe.org/3hvd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hvd RCSB], [http://www.ebi.ac.uk/pdbsum/3hvd PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hvd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3hvd" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus anthracis]]
	[[Category: Berger, J M]]		[[Category: Berger, J M]]
	[[Category: Dong, K C]]		[[Category: Dong, K C]]
Line 38:		Line 38:
	[[Category: Krantz, B A]]		[[Category: Krantz, B A]]
	[[Category: Anthrax protective antigen]]		[[Category: Anthrax protective antigen]]
		+	[[Category: Bacillus anthracis]]
	[[Category: Octamer]]		[[Category: Octamer]]
	[[Category: Toxin]]		[[Category: Toxin]]
	[[Category: Transport protein]]		[[Category: Transport protein]]
	[[Category: Xray-crystallography]]		[[Category: Xray-crystallography]]

OCA at 09:15, 20 January 2015

OCA — Tue, 20 Jan 2015 09:15:09 GMT

←Older revision		Revision as of 09:15, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3hvd]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HVD FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hvd]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HVD FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pa, PAG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis]), pa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pa, PAG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis]), pa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hvd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hvd RCSB], [http://www.ebi.ac.uk/pdbsum/3hvd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hvd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hvd RCSB], [http://www.ebi.ac.uk/pdbsum/3hvd PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
-	[[Category: Berger, J M.]]	+	[[Category: Berger, J M]]
-	[[Category: Dong, K C.]]	+	[[Category: Dong, K C]]
-	[[Category: Kintzer, A F.]]	+	[[Category: Kintzer, A F]]
-	[[Category: Krantz, B A.]]	+	[[Category: Krantz, B A]]
	[[Category: Anthrax protective antigen]]		[[Category: Anthrax protective antigen]]
-	[[Category: Bacillus anthracis]]
	[[Category: Octamer]]		[[Category: Octamer]]
	[[Category: Toxin]]		[[Category: Toxin]]
	[[Category: Transport protein]]		[[Category: Transport protein]]
	[[Category: Xray-crystallography]]		[[Category: Xray-crystallography]]

OCA at 13:02, 29 September 2014

OCA — Mon, 29 Sep 2014 13:02:52 GMT

←Older revision		Revision as of 13:02, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3hvd.~~png~~\|~~left~~\|200px]]	+	==The Protective Antigen Component of Anthrax Toxin Forms Functional Octameric Complexes==
		+	<StructureSection load='3hvd' size='340' side='right' caption='[[3hvd]], [[Resolution\|resolution]] 3.21Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3hvd]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HVD FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pa, PAG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis]), pa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hvd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hvd RCSB], [http://www.ebi.ac.uk/pdbsum/3hvd PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hv/3hvd_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The assembly of bacterial toxins and virulence factors is critical to their function, but the regulation of assembly during infection has not been studied. We begin to address this question using anthrax toxin as a model. The protective antigen (PA) component of the toxin assembles into ring-shaped homooligomers that bind the two other enzyme components of the toxin, lethal factor (LF) and edema factor (EF), to form toxic complexes. To disrupt the host, these toxic complexes are endocytosed, such that the PA oligomer forms a membrane-spanning channel that LF and EF translocate through to enter the cytosol. Using single-channel electrophysiology, we show that PA channels contain two populations of conductance states, which correspond to two different PA pre-channel oligomers observed by electron microscopy-the well-described heptamer and a novel octamer. Mass spectrometry demonstrates that the PA octamer binds four LFs, and assembly routes leading to the octamer are populated with even-numbered, dimeric and tetrameric, PA intermediates. Both heptameric and octameric PA complexes can translocate LF and EF with similar rates and efficiencies. Here, we report a 3.2-A crystal structure of the PA octamer. The octamer comprises approximately 20-30% of the oligomers on cells, but outside of the cell, the octamer is more stable than the heptamer under physiological pH. Thus, the PA octamer is a physiological, stable, and active assembly state capable of forming lethal toxins that may withstand the hostile conditions encountered in the bloodstream. This assembly mechanism may provide a novel means to control cytotoxicity.

-	~~{{STRUCTURE_3hvd\| PDB=3hvd \| SCENE= }}~~	+	The protective antigen component of anthrax toxin forms functional octameric complexes.,Kintzer AF, Thoren KL, Sterling HJ, Dong KC, Feld GK, Tang II, Zhang TT, Williams ER, Berger JM, Krantz BA J Mol Biol. 2009 Sep 25;392(3):614-29. Epub 2009 Jul 20. PMID:19627991<ref>PMID:19627991</ref>

-	~~===The Protective Antigen Component~~ of ~~Anthrax Toxin Forms Functional Octameric Complexes===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19627991}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3hvd]] is a 8 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_anthracis Bacillus anthracis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA].~~	+

	==See Also==		==See Also==
	*[[Anthrax protective antigen\|Anthrax protective antigen]]		*[[Anthrax protective antigen\|Anthrax protective antigen]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019627991<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Berger, J M.]]		[[Category: Berger, J M.]]

OCA at 12:05, 20 October 2012

OCA — Sat, 20 Oct 2012 12:05:29 GMT

←Older revision		Revision as of 12:05, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3hvd]] is a 8 chain structure ~~of [[Anthrax protective antigen]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA].	+	[[3hvd]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA].

	==See Also==		==See Also==

OCA: Protected "3hvd" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 07:35:14 GMT

Protected "3hvd" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:35, 26 July 2012

OCA at 07:35, 26 July 2012

OCA — Thu, 26 Jul 2012 07:35:12 GMT

←Older revision		Revision as of 07:35, 26 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3hvd.png\|left\|200px]]		[[Image:3hvd.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3hvd", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3hvd\| PDB=3hvd \| SCENE= }}		{{STRUCTURE_3hvd\| PDB=3hvd \| SCENE= }}

	===The Protective Antigen Component of Anthrax Toxin Forms Functional Octameric Complexes===		===The Protective Antigen Component of Anthrax Toxin Forms Functional Octameric Complexes===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19627991}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19627991 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19627991}}		{{ABSTRACT_PUBMED_19627991}}

	==About this Structure==		==About this Structure==
-	~~3HVD~~ is a 8 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA].	+	[[3hvd]] is a 8 chain structure of [[Anthrax protective antigen]] with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HVD OCA].
		+
		+	==See Also==
		+	*[[Anthrax protective antigen\|Anthrax protective antigen]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19627991~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019627991</ref><references group="xtra"/>
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Berger, J M.]]		[[Category: Berger, J M.]]
Line 35:		Line 26:
	[[Category: Transport protein]]		[[Category: Transport protein]]
	[[Category: Xray-crystallography]]		[[Category: Xray-crystallography]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Sep 21 19:28:11 2009''