3hxe - Revision history

OCA at 10:02, 21 February 2024

OCA — Wed, 21 Feb 2024 10:02:15 GMT

←Older revision		Revision as of 10:02, 21 February 2024
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	<StructureSection load='3hxe' size='340' side='right'caption='[[3hxe]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3hxe' size='340' side='right'caption='[[3hxe]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hxe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HXE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hxe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HXE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BD8:N-UNDECANOYL-L-HISTIDYL-L-PHENYLALANYL-N-METHYL-N-(2-PYRIDIN-2-YLETHYL)-L-TYROSINAMIDE'>BD8</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3hxb\|3hxb]], [[3hxc\|3hxc]], [[3hxd\|3hxd]], [[3hxf\|3hxf]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BD8:N-UNDECANOYL-L-HISTIDYL-L-PHENYLALANYL-N-METHYL-N-(2-PYRIDIN-2-YLETHYL)-L-TYROSINAMIDE'>BD8</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Protein_geranylgeranyltransferase_type_II Protein geranylgeranyltransferase type II], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.60 2.5.1.60] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hxe OCA], [https://pdbe.org/3hxe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hxe RCSB], [https://www.ebi.ac.uk/pdbsum/3hxe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hxe ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hxe OCA], [https://pdbe.org/3hxe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hxe RCSB], [https://www.ebi.ac.uk/pdbsum/3hxe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hxe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PGTA_RAT PGTA_RAT]] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively. [[https://www.uniprot.org/uniprot/PGTB2_RAT PGTB2_RAT]] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively.	+	[https://www.uniprot.org/uniprot/PGTA_RAT PGTA_RAT] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hxe ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hxe ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Rab geranylgeranyl transferase (RabGGTase) catalyzes the attachment of geranylgeranyl isoprenoids to Rab guanine triphosphatases, which are key regulators in vesicular transport. Because geranylgeranylation is required for proper function and overexpression of Rabs has been observed in various cancers, RabGGTase may be a target for novel therapeutics. The development of selective inhibitors is, however, difficult because two related enzymes involved in other cellular processes exist in eukaryotes and because RabGGTase recognizes protein substrates indirectly, resulting in relaxed specificity. We report the synthesis of a peptidic library based on the farnesyl transferase inhibitor pepticinnamin E. Of 469 compounds investigated, several were identified as selective for RabGGTase with low micromolar IC(50) values. The compounds were not generally cytotoxic and inhibited Rab isoprenylation in COS-7 cells. Crystal structure analysis revealed that selective inhibitors interact with a tunnel unique to RabGGTase, implying that this structural motif is an attractive target for improved RabGGTase inhibitors.
-
-	Design, synthesis, and characterization of peptide-based rab geranylgeranyl transferase inhibitors.,Tan KT, Guiu-Rozas E, Bon RS, Guo Z, Delon C, Wetzel S, Arndt S, Alexandrov K, Waldmann H, Goody RS, Wu YW, Blankenfeldt W J Med Chem. 2009 Dec 24;52(24):8025-37. PMID:19894725<ref>PMID:19894725</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3hxe" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Geranylgeranyl transferase 3D structures\|Geranylgeranyl transferase 3D structures]]		*[[Geranylgeranyl transferase 3D structures\|Geranylgeranyl transferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Buffalo rat]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Protein geranylgeranyltransferase type II~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: Alexandrov, K]]	+	[[Category: Alexandrov K]]
-	[[Category: Blankenfeldt, W]]	+	[[Category: Blankenfeldt W]]
-	[[Category: Goody~~, R S~~]]	+	[[Category: Goody RS]]
-	[[Category: Guo, Z]]	+	[[Category: Guo Z]]
-	[[Category: Waldmann, H]]	+	[[Category: Waldmann H]]
-	~~[[Category: Protein prenylation inhibition]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 11:59, 30 March 2022

OCA — Wed, 30 Mar 2022 11:59:34 GMT

←Older revision		Revision as of 11:59, 30 March 2022
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	<StructureSection load='3hxe' size='340' side='right'caption='[[3hxe]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3hxe' size='340' side='right'caption='[[3hxe]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hxe]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3HXE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hxe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HXE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BD8:N-UNDECANOYL-L-HISTIDYL-L-PHENYLALANYL-N-METHYL-N-(2-PYRIDIN-2-YLETHYL)-L-TYROSINAMIDE'>BD8</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BD8:N-UNDECANOYL-L-HISTIDYL-L-PHENYLALANYL-N-METHYL-N-(2-PYRIDIN-2-YLETHYL)-L-TYROSINAMIDE'>BD8</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hxb\|3hxb]], [[3hxc\|3hxc]], [[3hxd\|3hxd]], [[3hxf\|3hxf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3hxb\|3hxb]], [[3hxc\|3hxc]], [[3hxd\|3hxd]], [[3hxf\|3hxf]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Protein_geranylgeranyltransferase_type_II Protein geranylgeranyltransferase type II], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.60 2.5.1.60] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Protein_geranylgeranyltransferase_type_II Protein geranylgeranyltransferase type II], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.60 2.5.1.60] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3hxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hxe OCA], [~~http~~://pdbe.org/3hxe PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3hxe RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3hxe PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3hxe ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hxe OCA], [https://pdbe.org/3hxe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hxe RCSB], [https://www.ebi.ac.uk/pdbsum/3hxe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hxe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PGTA_RAT PGTA_RAT]] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively. [[~~http~~://www.uniprot.org/uniprot/PGTB2_RAT PGTB2_RAT]] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively.	+	[[https://www.uniprot.org/uniprot/PGTA_RAT PGTA_RAT]] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively. [[https://www.uniprot.org/uniprot/PGTB2_RAT PGTB2_RAT]] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:42, 28 August 2019

OCA — Wed, 28 Aug 2019 16:42:53 GMT

←Older revision		Revision as of 16:42, 28 August 2019
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	==Engineered RabGGTase in complex with a peptidomimetic inhibitor (compound 37)==		==Engineered RabGGTase in complex with a peptidomimetic inhibitor (compound 37)==
-	<StructureSection load='3hxe' size='340' side='right' caption='[[3hxe]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='3hxe' size='340' side='right'caption='[[3hxe]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3hxe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HXE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hxe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HXE FirstGlance]. <br>
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hx/3hxe_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hx/3hxe_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 3hxe" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3hxe" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Geranylgeranyl transferase 3D structures\|Geranylgeranyl transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
		+	[[Category: Large Structures]]
	[[Category: Protein geranylgeranyltransferase type II]]		[[Category: Protein geranylgeranyltransferase type II]]
	[[Category: Alexandrov, K]]		[[Category: Alexandrov, K]]

OCA at 05:11, 16 November 2017

OCA — Thu, 16 Nov 2017 05:11:40 GMT

←Older revision		Revision as of 05:11, 16 November 2017
Line 1:		Line 1:
		+
	==Engineered RabGGTase in complex with a peptidomimetic inhibitor (compound 37)==		==Engineered RabGGTase in complex with a peptidomimetic inhibitor (compound 37)==
	<StructureSection load='3hxe' size='340' side='right' caption='[[3hxe]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3hxe' size='340' side='right' caption='[[3hxe]], [[Resolution\|resolution]] 1.95Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hxb\|3hxb]], [[3hxc\|3hxc]], [[3hxd\|3hxd]], [[3hxf\|3hxf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hxb\|3hxb]], [[3hxc\|3hxc]], [[3hxd\|3hxd]], [[3hxf\|3hxf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_geranylgeranyltransferase_type_II Protein geranylgeranyltransferase type II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.60 2.5.1.60] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_geranylgeranyltransferase_type_II Protein geranylgeranyltransferase type II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.60 2.5.1.60] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hxe OCA], [http://pdbe.org/3hxe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hxe RCSB], [http://www.ebi.ac.uk/pdbsum/3hxe PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hxe OCA], [http://pdbe.org/3hxe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hxe RCSB], [http://www.ebi.ac.uk/pdbsum/3hxe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hxe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 3hxe" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3hxe" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Geranylgeranyl transferase\|Geranylgeranyl transferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 22:31, 9 February 2016

OCA — Tue, 09 Feb 2016 22:31:52 GMT

←Older revision		Revision as of 22:31, 9 February 2016
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	<StructureSection load='3hxe' size='340' side='right' caption='[[3hxe]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3hxe' size='340' side='right' caption='[[3hxe]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hxe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rattus_norvegicus Rattus norvegicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HXE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hxe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HXE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BD8:N-UNDECANOYL-L-HISTIDYL-L-PHENYLALANYL-N-METHYL-N-(2-PYRIDIN-2-YLETHYL)-L-TYROSINAMIDE'>BD8</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BD8:N-UNDECANOYL-L-HISTIDYL-L-PHENYLALANYL-N-METHYL-N-(2-PYRIDIN-2-YLETHYL)-L-TYROSINAMIDE'>BD8</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hxb\|3hxb]], [[3hxc\|3hxc]], [[3hxd\|3hxd]], [[3hxf\|3hxf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hxb\|3hxb]], [[3hxc\|3hxc]], [[3hxd\|3hxd]], [[3hxf\|3hxf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_geranylgeranyltransferase_type_II Protein geranylgeranyltransferase type II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.60 2.5.1.60] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_geranylgeranyltransferase_type_II Protein geranylgeranyltransferase type II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.60 2.5.1.60] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hxe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hxe RCSB], [http://www.ebi.ac.uk/pdbsum/3hxe PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hxe OCA], [http://pdbe.org/3hxe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hxe RCSB], [http://www.ebi.ac.uk/pdbsum/3hxe PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hxe ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3hxe" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Buffalo rat]]
	[[Category: Protein geranylgeranyltransferase type II]]		[[Category: Protein geranylgeranyltransferase type II]]
-	[[Category: Rattus norvegicus]]
	[[Category: Alexandrov, K]]		[[Category: Alexandrov, K]]
	[[Category: Blankenfeldt, W]]		[[Category: Blankenfeldt, W]]

OCA at 09:58, 25 December 2014

OCA — Thu, 25 Dec 2014 09:58:54 GMT

←Older revision		Revision as of 09:58, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hxe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hxe RCSB], [http://www.ebi.ac.uk/pdbsum/3hxe PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hxe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hxe RCSB], [http://www.ebi.ac.uk/pdbsum/3hxe PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PGTA_RAT PGTA_RAT]] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively. [[http://www.uniprot.org/uniprot/PGTB2_RAT PGTB2_RAT]] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:21, 8 December 2014

OCA — Mon, 08 Dec 2014 09:21:40 GMT

←Older revision		Revision as of 09:21, 8 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3hxe.~~png~~\|~~left~~\|200px]]	+	==Engineered RabGGTase in complex with a peptidomimetic inhibitor (compound 37)==
		+	<StructureSection load='3hxe' size='340' side='right' caption='[[3hxe]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3hxe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HXE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BD8:N-UNDECANOYL-L-HISTIDYL-L-PHENYLALANYL-N-METHYL-N-(2-PYRIDIN-2-YLETHYL)-L-TYROSINAMIDE'>BD8</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hxb\|3hxb]], [[3hxc\|3hxc]], [[3hxd\|3hxd]], [[3hxf\|3hxf]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_geranylgeranyltransferase_type_II Protein geranylgeranyltransferase type II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.60 2.5.1.60] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hxe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hxe RCSB], [http://www.ebi.ac.uk/pdbsum/3hxe PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hx/3hxe_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rab geranylgeranyl transferase (RabGGTase) catalyzes the attachment of geranylgeranyl isoprenoids to Rab guanine triphosphatases, which are key regulators in vesicular transport. Because geranylgeranylation is required for proper function and overexpression of Rabs has been observed in various cancers, RabGGTase may be a target for novel therapeutics. The development of selective inhibitors is, however, difficult because two related enzymes involved in other cellular processes exist in eukaryotes and because RabGGTase recognizes protein substrates indirectly, resulting in relaxed specificity. We report the synthesis of a peptidic library based on the farnesyl transferase inhibitor pepticinnamin E. Of 469 compounds investigated, several were identified as selective for RabGGTase with low micromolar IC(50) values. The compounds were not generally cytotoxic and inhibited Rab isoprenylation in COS-7 cells. Crystal structure analysis revealed that selective inhibitors interact with a tunnel unique to RabGGTase, implying that this structural motif is an attractive target for improved RabGGTase inhibitors.

-	~~{{STRUCTURE_3hxe\| PDB=3hxe \| SCENE= }}~~	+	Design, synthesis, and characterization of peptide-based rab geranylgeranyl transferase inhibitors.,Tan KT, Guiu-Rozas E, Bon RS, Guo Z, Delon C, Wetzel S, Arndt S, Alexandrov K, Waldmann H, Goody RS, Wu YW, Blankenfeldt W J Med Chem. 2009 Dec 24;52(24):8025-37. PMID:19894725<ref>PMID:19894725</ref>

-	~~===Engineered RabGGTase in complex with~~ a ~~peptidomimetic inhibitor (compound 37)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_19894725}}~~	+	==See Also==
-		+	*[[Geranylgeranyl transferase\|Geranylgeranyl transferase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~3hxe~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:019894725<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Protein geranylgeranyltransferase type II]]		[[Category: Protein geranylgeranyltransferase type II]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
-	[[Category: Alexandrov, K.]]	+	[[Category: Alexandrov, K]]
-	[[Category: Blankenfeldt, W.]]	+	[[Category: Blankenfeldt, W]]
-	[[Category: Goody, R S.]]	+	[[Category: Goody, R S]]
-	[[Category: Guo, Z.]]	+	[[Category: Guo, Z]]
-	[[Category: Waldmann, H.]]	+	[[Category: Waldmann, H]]
	[[Category: Protein prenylation inhibition]]		[[Category: Protein prenylation inhibition]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA: Protected "3hxe" [edit=sysop:move=sysop]

OCA — Wed, 13 Feb 2013 09:26:43 GMT

Protected "3hxe" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:26, 13 February 2013

OCA at 09:26, 13 February 2013

OCA — Wed, 13 Feb 2013 09:26:42 GMT

←Older revision		Revision as of 09:26, 13 February 2013
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-	{{Seed}}
	[[Image:3hxe.png\|left\|200px]]		[[Image:3hxe.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3hxe", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3hxe\| PDB=3hxe \| SCENE= }}		{{STRUCTURE_3hxe\| PDB=3hxe \| SCENE= }}

	===Engineered RabGGTase in complex with a peptidomimetic inhibitor (compound 37)===		===Engineered RabGGTase in complex with a peptidomimetic inhibitor (compound 37)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19894725}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19894725 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19894725}}		{{ABSTRACT_PUBMED_19894725}}

	==About this Structure==		==About this Structure==
-	~~3HXE~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA].	+	[[3hxe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19894725~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019894725</ref><references group="xtra"/>
	[[Category: Protein geranylgeranyltransferase type II]]		[[Category: Protein geranylgeranyltransferase type II]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
Line 33:		Line 21:
	[[Category: Protein prenylation inhibition]]		[[Category: Protein prenylation inhibition]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 30 14:17:32 2009''

OCA at 12:17, 30 December 2009

OCA — Wed, 30 Dec 2009 12:17:33 GMT

←Older revision		Revision as of 12:17, 30 December 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3hxe.~~jpg~~\|left\|200px]]	+	[[Image:3hxe.png\|left\|200px]]

	<!--		<!--
Line 10:		Line 10:
	{{STRUCTURE_3hxe\| PDB=3hxe \| SCENE= }}		{{STRUCTURE_3hxe\| PDB=3hxe \| SCENE= }}

-	===Engineered RabGGTase in complex with a peptidomimetic inhibitor (compound 32)===	+	===Engineered RabGGTase in complex with a peptidomimetic inhibitor (compound 37)===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19894725}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19894725 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19894725}}

	==About this Structure==		==About this Structure==
	3HXE is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA].		3HXE is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXE OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19894725</ref><references group="xtra"/>
	[[Category: Protein geranylgeranyltransferase type II]]		[[Category: Protein geranylgeranyltransferase type II]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
Line 25:		Line 34:
	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 9 09~~:48:35 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 30 14:17:32 2009''