3hzq - Revision history

OCA at 10:02, 21 February 2024

OCA — Wed, 21 Feb 2024 10:02:36 GMT

←Older revision		Revision as of 10:02, 21 February 2024
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	<StructureSection load='3hzq' size='340' side='right'caption='[[3hzq]], [[Resolution\|resolution]] 3.82Å' scene=''>		<StructureSection load='3hzq' size='340' side='right'caption='[[3hzq]], [[Resolution\|resolution]] 3.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Staaw Staaw~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_MW2 Staphylococcus aureus subsp. aureus MW2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~mscL (~~[~~https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=196620 STAAW])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.82Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [https://pdbe.org/3hzq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [https://www.ebi.ac.uk/pdbsum/3hzq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hzq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [https://pdbe.org/3hzq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [https://www.ebi.ac.uk/pdbsum/3hzq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hzq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MSCL_STAAW MSCL_STAAW]] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell.	+	[https://www.uniprot.org/uniprot/MSCL_STAAW MSCL_STAAW] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hzq ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hzq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The ability of cells to sense and respond to mechanical force underlies diverse processes such as touch and hearing in animals, gravitropism in plants, and bacterial osmoregulation. In bacteria, mechanosensation is mediated by the mechanosensitive channels of large (MscL), small (MscS), potassium-dependent (MscK) and mini (MscM) conductances. These channels act as 'emergency relief valves' protecting bacteria from lysis upon acute osmotic down-shock. Among them, MscL has been intensively studied since the original identification and characterization 15 years ago. MscL is reversibly and directly gated by changes in membrane tension. In the open state, MscL forms a non-selective 3 nS conductance channel which gates at tensions close to the lytic limit of the bacterial membrane. An earlier crystal structure at 3.5 A resolution of a pentameric MscL from Mycobacterium tuberculosis represents a closed-state or non-conducting conformation. MscL has a complex gating behaviour; it exhibits several intermediates between the closed and open states, including one putative non-conductive expanded state and at least three sub-conducting states. Although our understanding of the closed and open states of MscL has been increasing, little is known about the structures of the intermediate states despite their importance in elucidating the complete gating process of MscL. Here we present the crystal structure of a carboxy-terminal truncation mutant (Delta95-120) of MscL from Staphylococcus aureus (SaMscL(CDelta26)) at 3.8 A resolution. Notably, SaMscL(CDelta26) forms a tetrameric channel with both transmembrane helices tilted away from the membrane normal at angles close to that inferred for the open state, probably corresponding to a non-conductive but partially expanded intermediate state.
-
-	Structure of a tetrameric MscL in an expanded intermediate state.,Liu Z, Gandhi CS, Rees DC Nature. 2009 Sep 3;461(7260):120-4. Epub 2009 Aug 23. PMID:19701184<ref>PMID:19701184</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3hzq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ion channels 3D structures\|Ion channels 3D structures]]		*[[Ion channels 3D structures\|Ion channels 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Staaw~~]]	+	[[Category: Staphylococcus aureus subsp. aureus MW2]]
-	[[Category: Gandhi~~, C S~~]]	+	[[Category: Gandhi CS]]
-	[[Category: Liu~~, Z F~~]]	+	[[Category: Liu ZF]]
-	[[Category: Rees~~, D C]]~~	+	[[Category: Rees DC]]
-	~~[[Category: Cell membrane]]~~	+
-	~~[[Category: Intermediate state mechanosensitive channel osmoregulation]]~~	+
-	~~[[Category: Ion transport]]~~	+
-	~~[[Category: Ionic channel]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Transmembrane]]~~	+
-	~~[[Category: Transport~~]]	+

OCA at 12:06, 13 October 2021

OCA — Wed, 13 Oct 2021 12:06:14 GMT

←Older revision		Revision as of 12:06, 13 October 2021
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	==Structure of a tetrameric MscL in an expanded intermediate state==		==Structure of a tetrameric MscL in an expanded intermediate state==
-	<StructureSection load='3hzq' size='340' side='right' caption='[[3hzq]], [[Resolution\|resolution]] 3.82Å' scene=''>	+	<StructureSection load='3hzq' size='340' side='right'caption='[[3hzq]], [[Resolution\|resolution]] 3.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Staaw Staaw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staaw Staaw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mscL ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196620 STAAW])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mscL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196620 STAAW])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [~~http~~://pdbe.org/3hzq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3hzq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3hzq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [https://pdbe.org/3hzq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [https://www.ebi.ac.uk/pdbsum/3hzq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hzq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MSCL_STAAW MSCL_STAAW]] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell.	+	[[https://www.uniprot.org/uniprot/MSCL_STAAW MSCL_STAAW]] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hz/3hzq_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hz/3hzq_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 3hzq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3hzq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Staaw]]		[[Category: Staaw]]
	[[Category: Gandhi, C S]]		[[Category: Gandhi, C S]]

OCA at 07:53, 1 November 2017

OCA — Wed, 01 Nov 2017 07:53:20 GMT

←Older revision		Revision as of 07:53, 1 November 2017
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		+
	==Structure of a tetrameric MscL in an expanded intermediate state==		==Structure of a tetrameric MscL in an expanded intermediate state==
	<StructureSection load='3hzq' size='340' side='right' caption='[[3hzq]], [[Resolution\|resolution]] 3.82Å' scene=''>		<StructureSection load='3hzq' size='340' side='right' caption='[[3hzq]], [[Resolution\|resolution]] 3.82Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staaw Staaw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staaw Staaw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mscL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196620 STAAW])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mscL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196620 STAAW])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [http://pdbe.org/3hzq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [http://www.ebi.ac.uk/pdbsum/3hzq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [http://pdbe.org/3hzq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [http://www.ebi.ac.uk/pdbsum/3hzq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hzq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 3hzq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3hzq" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Ion channels\|Ion channels]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:09, 10 February 2016

OCA — Wed, 10 Feb 2016 05:09:05 GMT

←Older revision		Revision as of 05:09, 10 February 2016
Line 2:		Line 2:
	<StructureSection load='3hzq' size='340' side='right' caption='[[3hzq]], [[Resolution\|resolution]] 3.82Å' scene=''>		<StructureSection load='3hzq' size='340' side='right' caption='[[3hzq]], [[Resolution\|resolution]] 3.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_subsp._aureus_mw2 Staphylococcus aureus subsp. aureus mw2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staaw Staaw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mscL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196620 ~~Staphylococcus aureus subsp. aureus MW2~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mscL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196620 STAAW])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [http://www.ebi.ac.uk/pdbsum/3hzq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [http://pdbe.org/3hzq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [http://www.ebi.ac.uk/pdbsum/3hzq PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hzq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3hzq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Staphylococcus aureus subsp. aureus mw2~~]]	+	[[Category: Staaw]]
	[[Category: Gandhi, C S]]		[[Category: Gandhi, C S]]
	[[Category: Liu, Z F]]		[[Category: Liu, Z F]]

OCA at 12:00, 25 December 2014

OCA — Thu, 25 Dec 2014 12:00:21 GMT

←Older revision		Revision as of 12:00, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mw2 Staphylococcus aureus subsp. aureus mw2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mw2 Staphylococcus aureus subsp. aureus mw2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mscL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196620 Staphylococcus aureus subsp. aureus MW2])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mscL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196620 Staphylococcus aureus subsp. aureus MW2])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [http://www.ebi.ac.uk/pdbsum/3hzq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [http://www.ebi.ac.uk/pdbsum/3hzq PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MSCL_STAAW MSCL_STAAW]] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Staphylococcus aureus subsp. aureus mw2]]		[[Category: Staphylococcus aureus subsp. aureus mw2]]
-	[[Category: Gandhi, C S.]]	+	[[Category: Gandhi, C S]]
-	[[Category: Liu, Z F.]]	+	[[Category: Liu, Z F]]
-	[[Category: Rees, D C.]]	+	[[Category: Rees, D C]]
	[[Category: Cell membrane]]		[[Category: Cell membrane]]
	[[Category: Intermediate state mechanosensitive channel osmoregulation]]		[[Category: Intermediate state mechanosensitive channel osmoregulation]]

OCA at 13:21, 29 September 2014

OCA — Mon, 29 Sep 2014 13:21:16 GMT

←Older revision		Revision as of 13:21, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:3hzq.~~png\|left~~\|200px]]	+	==Structure of a tetrameric MscL in an expanded intermediate state==
		+	<StructureSection load='3hzq' size='340' side='right' caption='[[3hzq]], [[Resolution\|resolution]] 3.82Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mw2 Staphylococcus aureus subsp. aureus mw2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mscL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196620 Staphylococcus aureus subsp. aureus MW2])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [http://www.ebi.ac.uk/pdbsum/3hzq PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hz/3hzq_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ability of cells to sense and respond to mechanical force underlies diverse processes such as touch and hearing in animals, gravitropism in plants, and bacterial osmoregulation. In bacteria, mechanosensation is mediated by the mechanosensitive channels of large (MscL), small (MscS), potassium-dependent (MscK) and mini (MscM) conductances. These channels act as 'emergency relief valves' protecting bacteria from lysis upon acute osmotic down-shock. Among them, MscL has been intensively studied since the original identification and characterization 15 years ago. MscL is reversibly and directly gated by changes in membrane tension. In the open state, MscL forms a non-selective 3 nS conductance channel which gates at tensions close to the lytic limit of the bacterial membrane. An earlier crystal structure at 3.5 A resolution of a pentameric MscL from Mycobacterium tuberculosis represents a closed-state or non-conducting conformation. MscL has a complex gating behaviour; it exhibits several intermediates between the closed and open states, including one putative non-conductive expanded state and at least three sub-conducting states. Although our understanding of the closed and open states of MscL has been increasing, little is known about the structures of the intermediate states despite their importance in elucidating the complete gating process of MscL. Here we present the crystal structure of a carboxy-terminal truncation mutant (Delta95-120) of MscL from Staphylococcus aureus (SaMscL(CDelta26)) at 3.8 A resolution. Notably, SaMscL(CDelta26) forms a tetrameric channel with both transmembrane helices tilted away from the membrane normal at angles close to that inferred for the open state, probably corresponding to a non-conductive but partially expanded intermediate state.

-	~~{{STRUCTURE_3hzq\| PDB=3hzq \| SCENE= }}~~	+	Structure of a tetrameric MscL in an expanded intermediate state.,Liu Z, Gandhi CS, Rees DC Nature. 2009 Sep 3;461(7260):120-4. Epub 2009 Aug 23. PMID:19701184<ref>PMID:19701184</ref>

-	~~===Structure~~ of ~~a tetrameric MscL in an expanded intermediate state===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19701184}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3hzq]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Staphylococcus_aureus_subsp~~.~~_aureus_mw2 Staphylococcus aureus subsp. aureus mw2]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA].~~	+

	==See Also==		==See Also==
	*[[Ion channels\|Ion channels]]		*[[Ion channels\|Ion channels]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019701184<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Staphylococcus aureus subsp. aureus mw2]]		[[Category: Staphylococcus aureus subsp. aureus mw2]]
	[[Category: Gandhi, C S.]]		[[Category: Gandhi, C S.]]

OCA at 16:26, 20 October 2012

OCA — Sat, 20 Oct 2012 16:26:17 GMT

←Older revision		Revision as of 16:26, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3hzq]] is a 1 chain structure ~~of [[Ion channels]]~~ with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mw2 Staphylococcus aureus subsp. aureus mw2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA].	+	[[3hzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mw2 Staphylococcus aureus subsp. aureus mw2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA].

	==See Also==		==See Also==

OCA at 18:58, 6 August 2012

OCA — Mon, 06 Aug 2012 18:58:27 GMT

←Older revision		Revision as of 18:58, 6 August 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3hzq]] is a 1 chain structure of ~~[[]] and~~ [[Ion channels]] with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mw2 Staphylococcus aureus subsp. aureus mw2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA].	+	[[3hzq]] is a 1 chain structure of [[Ion channels]] with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mw2 Staphylococcus aureus subsp. aureus mw2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA].

	==See Also==		==See Also==
-	*[[\|]]
	*[[Ion channels\|Ion channels]]		*[[Ion channels\|Ion channels]]

OCA at 18:53, 6 August 2012

OCA — Mon, 06 Aug 2012 18:53:15 GMT

←Older revision		Revision as of 18:53, 6 August 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3hzq]] is a 1 chain structure of [[~~Ion channels~~]] and [[Ion channels ~~(Part II)~~]] with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mw2 Staphylococcus aureus subsp. aureus mw2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA].	+	[[3hzq]] is a 1 chain structure of [[]] and [[Ion channels]] with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mw2 Staphylococcus aureus subsp. aureus mw2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA].

	==See Also==		==See Also==
		+	*[[\|]]
	*[[Ion channels\|Ion channels]]		*[[Ion channels\|Ion channels]]
-	*[[Ion channels (Part II)\|Ion channels (Part II)]]

	==Reference==		==Reference==

OCA at 21:41, 26 July 2012

OCA — Thu, 26 Jul 2012 21:41:26 GMT

←Older revision		Revision as of 21:41, 26 July 2012
Line 1:		Line 1:
	[[Image:3hzq.png\|left\|200px]]		[[Image:3hzq.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3hzq", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3hzq\| PDB=3hzq \| SCENE= }}		{{STRUCTURE_3hzq\| PDB=3hzq \| SCENE= }}

	===Structure of a tetrameric MscL in an expanded intermediate state===		===Structure of a tetrameric MscL in an expanded intermediate state===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19701184}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19701184 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19701184}}		{{ABSTRACT_PUBMED_19701184}}

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	==See Also==		==See Also==
-	*[[Ion channels]]	+	*[[Ion channels\|Ion channels]]
-	*[[Ion channels (Part II)]]	+	*[[Ion channels (Part II)\|Ion channels (Part II)]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19701184~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019701184</ref><references group="xtra"/>
	[[Category: Staphylococcus aureus subsp. aureus mw2]]		[[Category: Staphylococcus aureus subsp. aureus mw2]]
	[[Category: Gandhi, C S.]]		[[Category: Gandhi, C S.]]