3i27 - Revision history

OCA at 15:55, 1 November 2023

OCA — Wed, 01 Nov 2023 15:55:06 GMT

←Older revision		Revision as of 15:55, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3i27' size='340' side='right'caption='[[3i27]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3i27' size='340' side='right'caption='[[3i27]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i27]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Brv-1 Brv-~~1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I27 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i27]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Breda_virus_serotype_1 Breda virus serotype 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I27 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SID:METHYL+9-S-ACETYL-5-(ACETYLAMINO)-3,5-DIDEOXY-9-THIO-D-GLYCERO-ALPHA-D-GALACTO-NON-2-ULOPYRANOSIDONIC+ACID'>SID</scene~~>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3i1k\|3i1k]], [[3i1l\|3i1l]], [[3i26\|3i26]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SID:METHYL+9-S-ACETYL-5-(ACETYLAMINO)-3,5-DIDEOXY-9-THIO-D-GLYCERO-ALPHA-D-GALACTO-NON-2-ULOPYRANOSIDONIC+ACID'>SID</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360393 BRV-1])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Sialate_O-acetylesterase Sialate O-acetylesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.53 3.1.1.53] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i27 OCA], [https://pdbe.org/3i27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i27 RCSB], [https://www.ebi.ac.uk/pdbsum/3i27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i27 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i27 OCA], [https://pdbe.org/3i27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i27 RCSB], [https://www.ebi.ac.uk/pdbsum/3i27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i27 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HEMA_BRV1 HEMA_BRV1]] Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-di-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. Also hydrolyzes 5-N-acetyl-4-O-acetylneuramic acid and N-acetyl-9-O-acetylneuraminic acid, but displays a substrate preference for N-acetyl-9-di-O-acetylneuraminic acid. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.	+	[https://www.uniprot.org/uniprot/HEMA_BRV1 HEMA_BRV1] Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-di-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. Also hydrolyzes 5-N-acetyl-4-O-acetylneuramic acid and N-acetyl-9-O-acetylneuraminic acid, but displays a substrate preference for N-acetyl-9-di-O-acetylneuraminic acid. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Brv-~~1]]	+	[[Category: Breda virus serotype 1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Sialate O-acetylesterase]]~~	+	[[Category: Huizinga EG]]
-	[[Category: Huizinga~~, E G~~]]	+	[[Category: Zeng QH]]
-	[[Category: Zeng~~, Q H]]~~	+
-	~~[[Category: Cell membrane]]~~	+
-	~~[[Category: Envelope protein]]~~	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Hemagglutinin]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Sgnh-hydrolase fold]]~~	+
-	~~[[Category: Swiss roll]]~~	+
-	~~[[Category: Transmembrane]]~~	+
-	~~[[Category: Virion~~]]	+

OCA at 11:20, 6 April 2022

OCA — Wed, 06 Apr 2022 11:20:33 GMT

←Older revision		Revision as of 11:20, 6 April 2022
Line 1:		Line 1:

	==Structure of bovine torovirus Hemagglutinin-Esterase in complex with receptor==		==Structure of bovine torovirus Hemagglutinin-Esterase in complex with receptor==
-	<StructureSection load='3i27' size='340' side='right' caption='[[3i27]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3i27' size='340' side='right'caption='[[3i27]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i27]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Brv-1 Brv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I27 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3I27 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i27]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Brv-1 Brv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I27 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA~~</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN~~</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SID:METHYL+9-S-ACETYL-5-(ACETYLAMINO)-3,5-DIDEOXY-9-THIO-D-GLYCERO-ALPHA-D-GALACTO-NON-2-ULOPYRANOSIDONIC+ACID'>SID</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SID:METHYL+9-S-ACETYL-5-(ACETYLAMINO)-3,5-DIDEOXY-9-THIO-D-GLYCERO-ALPHA-D-GALACTO-NON-2-ULOPYRANOSIDONIC+ACID'>SID</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i1k\|3i1k]], [[3i1l\|3i1l]], [[3i26\|3i26]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3i1k\|3i1k]], [[3i1l\|3i1l]], [[3i26\|3i26]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HE ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360393 BRV-1])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360393 BRV-1])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Sialate_O-acetylesterase Sialate O-acetylesterase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.53 3.1.1.53] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Sialate_O-acetylesterase Sialate O-acetylesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.53 3.1.1.53] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3i27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i27 OCA], [~~http~~://pdbe.org/3i27 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3i27 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3i27 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3i27 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i27 OCA], [https://pdbe.org/3i27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i27 RCSB], [https://www.ebi.ac.uk/pdbsum/3i27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i27 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HEMA_BRV1 HEMA_BRV1]] Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-di-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. Also hydrolyzes 5-N-acetyl-4-O-acetylneuramic acid and N-acetyl-9-O-acetylneuraminic acid, but displays a substrate preference for N-acetyl-9-di-O-acetylneuraminic acid. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.	+	[[https://www.uniprot.org/uniprot/HEMA_BRV1 HEMA_BRV1]] Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-di-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. Also hydrolyzes 5-N-acetyl-4-O-acetylneuramic acid and N-acetyl-9-O-acetylneuraminic acid, but displays a substrate preference for N-acetyl-9-di-O-acetylneuraminic acid. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:

	==See Also==		==See Also==
-	*[[Hemagglutinin-esterase\|Hemagglutinin-esterase]]	+	*[[Hemagglutinin-esterase 3D structures\|Hemagglutinin-esterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Brv-1]]		[[Category: Brv-1]]
		+	[[Category: Large Structures]]
	[[Category: Sialate O-acetylesterase]]		[[Category: Sialate O-acetylesterase]]
	[[Category: Huizinga, E G]]		[[Category: Huizinga, E G]]

OCA at 09:39, 19 December 2018

OCA — Wed, 19 Dec 2018 09:39:45 GMT

←Older revision		Revision as of 09:39, 19 December 2018
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3i27]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Brv-1 Brv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I27 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3i27]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Brv-1 Brv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I27 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SID:METHYL+9-S-ACETYL-5-(ACETYLAMINO)-3,5-DIDEOXY-9-THIO-D-GLYCERO-ALPHA-D-GALACTO-NON-2-ULOPYRANOSIDONIC+ACID'>SID~~</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SID:METHYL+9-S-ACETYL-5-(ACETYLAMINO)-3,5-DIDEOXY-9-THIO-D-GLYCERO-ALPHA-D-GALACTO-NON-2-ULOPYRANOSIDONIC+ACID'>SID</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i1k\|3i1k]], [[3i1l\|3i1l]], [[3i26\|3i26]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i1k\|3i1k]], [[3i1l\|3i1l]], [[3i26\|3i26]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360393 BRV-1])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360393 BRV-1])</td></tr>
Line 16:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i2/3i27_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i2/3i27_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 05:46, 5 August 2016

OCA — Fri, 05 Aug 2016 05:46:18 GMT

←Older revision		Revision as of 05:46, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of bovine torovirus Hemagglutinin-Esterase in complex with receptor==		==Structure of bovine torovirus Hemagglutinin-Esterase in complex with receptor==
	<StructureSection load='3i27' size='340' side='right' caption='[[3i27]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3i27' size='340' side='right' caption='[[3i27]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i27]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Breda_virus_serotype_1 Breda virus serotype~~ 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I27 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i27]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Brv-1 Brv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I27 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SID:METHYL+9-S-ACETYL-5-(ACETYLAMINO)-3,5-DIDEOXY-9-THIO-D-GLYCERO-ALPHA-D-GALACTO-NON-2-ULOPYRANOSIDONIC+ACID'>SID</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SID:METHYL+9-S-ACETYL-5-(ACETYLAMINO)-3,5-DIDEOXY-9-THIO-D-GLYCERO-ALPHA-D-GALACTO-NON-2-ULOPYRANOSIDONIC+ACID'>SID</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i1k\|3i1k]], [[3i1l\|3i1l]], [[3i26\|3i26]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i1k\|3i1k]], [[3i1l\|3i1l]], [[3i26\|3i26]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360393 ~~Breda virus serotype~~ 1])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360393 BRV-1])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sialate_O-acetylesterase Sialate O-acetylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.53 3.1.1.53] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sialate_O-acetylesterase Sialate O-acetylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.53 3.1.1.53] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i27 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i27 RCSB], [http://www.ebi.ac.uk/pdbsum/3i27 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i27 OCA], [http://pdbe.org/3i27 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i27 RCSB], [http://www.ebi.ac.uk/pdbsum/3i27 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3i27 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i27 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 30:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3i27" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Breda virus serotype~~ 1]]	+	[[Category: Brv-1]]
	[[Category: Sialate O-acetylesterase]]		[[Category: Sialate O-acetylesterase]]
	[[Category: Huizinga, E G]]		[[Category: Huizinga, E G]]

OCA at 16:15, 25 December 2014

OCA — Thu, 25 Dec 2014 16:15:29 GMT

←Older revision		Revision as of 16:15, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i27 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i27 RCSB], [http://www.ebi.ac.uk/pdbsum/3i27 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i27 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i27 RCSB], [http://www.ebi.ac.uk/pdbsum/3i27 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HEMA_BRV1 HEMA_BRV1]] Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-di-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. Also hydrolyzes 5-N-acetyl-4-O-acetylneuramic acid and N-acetyl-9-O-acetylneuraminic acid, but displays a substrate preference for N-acetyl-9-di-O-acetylneuraminic acid. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:36, 18 December 2014

OCA — Thu, 18 Dec 2014 06:36:05 GMT

←Older revision		Revision as of 06:36, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3i27\| PDB=3i27 \| SCENE= }}~~	+	==Structure of bovine torovirus Hemagglutinin-Esterase in complex with receptor==
-	===Structure of bovine torovirus Hemagglutinin-Esterase in complex with receptor===	+	<StructureSection load='3i27' size='340' side='right' caption='[[3i27]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19721004}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3i27]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Breda_virus_serotype_1 Breda virus serotype 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I27 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SID:METHYL+9-S-ACETYL-5-(ACETYLAMINO)-3,5-DIDEOXY-9-THIO-D-GLYCERO-ALPHA-D-GALACTO-NON-2-ULOPYRANOSIDONIC+ACID'>SID</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i1k\|3i1k]], [[3i1l\|3i1l]], [[3i26\|3i26]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360393 Breda virus serotype 1])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sialate_O-acetylesterase Sialate O-acetylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.53 3.1.1.53] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i27 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i27 RCSB], [http://www.ebi.ac.uk/pdbsum/3i27 PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i2/3i27_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hemagglutinin esterases (HEs), closely related envelope glycoproteins in influenza C and corona- and toroviruses, mediate reversible attachment to O-acetylated sialic acids (Sias). They do so by acting both as lectins and as receptor-destroying enzymes, functions exerted by separate protein domains. HE divergence was accompanied by changes in quaternary structure and in receptor and substrate specificity. The selective forces underlying HE diversity and the molecular basis for Sia specificity are poorly understood. Here we present crystal structures of porcine and bovine torovirus HEs in complex with receptor analogs. Torovirus HEs form homodimers with sialate-O-acetylesterase domains almost identical to corresponding domains in orthomyxo- and coronavirus HEs, but with unique lectin sites. Structure-guided biochemical analysis of the esterase domains revealed that a functionally, but not structurally conserved arginine-Sia carboxylate interaction is critical for the binding and positioning of glycosidically bound Sias in the catalytic pocket. Although essential for efficient de-O-acetylation of Sias, this interaction is not required for catalysis nor does it affect substrate specificity. In fact, the distinct preference of the porcine torovirus enzyme for 9-mono- over 7,9-di-O-acetylated Sias can be explained from a single-residue difference with HEs of more promiscuous specificity. Apparently, esterase and lectin pockets coevolved; also the porcine torovirus HE receptor-binding site seems to have been designed to use 9-mono- and exclude di-O-acetylated Sias, possibly as an adaptation to replication in swine. Our findings shed light on HE evolution and provide fundamental insight into mechanisms of substrate binding, substrate recognition, and receptor selection in this important class of virion proteins.

-	~~==About this Structure==~~	+	Structural basis for ligand and substrate recognition by torovirus hemagglutinin esterases.,Langereis MA, Zeng Q, Gerwig GJ, Frey B, von Itzstein M, Kamerling JP, de Groot RJ, Huizinga EG Proc Natl Acad Sci U S A. 2009 Aug 31. PMID:19721004<ref>PMID:19721004</ref>
-	~~[[3i27]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Breda_virus_serotype_1 Breda virus serotype 1]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3I27 OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Hemagglutinin-esterase\|Hemagglutinin-esterase]]		*[[Hemagglutinin-esterase\|Hemagglutinin-esterase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019721004<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Breda virus serotype 1]]		[[Category: Breda virus serotype 1]]
	[[Category: Sialate O-acetylesterase]]		[[Category: Sialate O-acetylesterase]]
-	[[Category: Huizinga, E G.]]	+	[[Category: Huizinga, E G]]
-	[[Category: Zeng, Q H.]]	+	[[Category: Zeng, Q H]]
	[[Category: Cell membrane]]		[[Category: Cell membrane]]
	[[Category: Envelope protein]]		[[Category: Envelope protein]]

OCA: Protected "3i27" [edit=sysop:move=sysop]

OCA — Wed, 13 Mar 2013 11:08:18 GMT

Protected "3i27" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:08, 13 March 2013

OCA at 11:08, 13 March 2013

OCA — Wed, 13 Mar 2013 11:08:15 GMT

←Older revision		Revision as of 11:08, 13 March 2013
Line 1:		Line 1:
-	{{Seed}}
-	[[Image:3i27.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3i27", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3i27\| PDB=3i27 \| SCENE= }}		{{STRUCTURE_3i27\| PDB=3i27 \| SCENE= }}
-
	===Structure of bovine torovirus Hemagglutinin-Esterase in complex with receptor===		===Structure of bovine torovirus Hemagglutinin-Esterase in complex with receptor===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19721004}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19721004 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19721004}}		{{ABSTRACT_PUBMED_19721004}}

	==About this Structure==		==About this Structure==
-	~~3I27~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Breda_virus_serotype_1 Breda virus serotype 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I27 OCA].	+	[[3i27]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Breda_virus_serotype_1 Breda virus serotype 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I27 OCA].
		+
		+	==See Also==
		+	*[[Hemagglutinin-esterase\|Hemagglutinin-esterase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19721004~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019721004</ref><references group="xtra"/>
	[[Category: Breda virus serotype 1]]		[[Category: Breda virus serotype 1]]
	[[Category: Sialate O-acetylesterase]]		[[Category: Sialate O-acetylesterase]]
Line 38:		Line 25:
	[[Category: Transmembrane]]		[[Category: Transmembrane]]
	[[Category: Virion]]		[[Category: Virion]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 23 08:14:41 2009''

OCA at 05:14, 23 September 2009

OCA — Wed, 23 Sep 2009 05:14:42 GMT

←Older revision		Revision as of 05:14, 23 September 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3i27.~~jpg~~\|left\|200px]]	+	[[Image:3i27.png\|left\|200px]]

	<!--		<!--
Line 39:		Line 39:
	[[Category: Virion]]		[[Category: Virion]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep ~~16 09~~:01:12 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 23 08:14:41 2009''

OCA at 06:01, 16 September 2009

OCA — Wed, 16 Sep 2009 06:01:15 GMT

←Older revision		Revision as of 06:01, 16 September 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3i27.jpg\|left\|200px]]

-	The ~~entry 3i27~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3i27", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3i27\| PDB=3i27 \| SCENE= }}

-	~~Authors: Zeng, Q.H., Huizinga, E.G.~~	+	===Structure of bovine torovirus Hemagglutinin-Esterase in complex with receptor===

-	Description: Structure of bovine torovirus Hemagglutinin-Esterase in complex with receptor

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Aug 5~~ 09:46:55 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19721004}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19721004 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19721004}}
		+
		+	==About this Structure==
		+	3I27 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Breda_virus_serotype_1 Breda virus serotype 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I27 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19721004</ref><references group="xtra"/>
		+	[[Category: Breda virus serotype 1]]
		+	[[Category: Sialate O-acetylesterase]]
		+	[[Category: Huizinga, E G.]]
		+	[[Category: Zeng, Q H.]]
		+	[[Category: Cell membrane]]
		+	[[Category: Envelope protein]]
		+	[[Category: Glycoprotein]]
		+	[[Category: Hemagglutinin]]
		+	[[Category: Hydrolase]]
		+	[[Category: Membrane]]
		+	[[Category: Sgnh-hydrolase fold]]
		+	[[Category: Swiss roll]]
		+	[[Category: Transmembrane]]
		+	[[Category: Virion]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 16 09:01:12 2009''