3i46 - Revision history

OCA at 06:39, 3 April 2024

OCA — Wed, 03 Apr 2024 06:39:04 GMT

←Older revision		Revision as of 06:39, 3 April 2024
Line 3:		Line 3:
	<StructureSection load='3i46' size='340' side='right'caption='[[3i46]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3i46' size='340' side='right'caption='[[3i46]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i46]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Staphylococcus_aureus_rn4220~~ Staphylococcus aureus ~~rn4220~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I46 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i46]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_RN4220 Staphylococcus aureus subsp. aureus RN4220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I46 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3i41\|3i41]], [[3i48\|3i48]], [[3i5v\|3i5v]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">hlb ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=561307 Staphylococcus aureus RN4220])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i46 OCA], [https://pdbe.org/3i46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i46 RCSB], [https://www.ebi.ac.uk/pdbsum/3i46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i46 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i46 OCA], [https://pdbe.org/3i46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i46 RCSB], [https://www.ebi.ac.uk/pdbsum/3i46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i46 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A7LAI8_STAAU A7LAI8_STAAU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i46 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i46 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Beta toxin is a neutral sphingomyelinase secreted by certain strains of Staphylococcus aureus. This virulence factor lyses erythrocytes in order to evade the host immune system as well as scavenge nutrients. The structure of beta toxin was determined at 2.4-A resolution using crystals that were merohedrally twinned. This structure is similar to that of the sphingomyelinases of Listeria ivanovii and Bacillus cereus. Beta toxin belongs to the DNase I folding superfamily; in addition to sphingomyelinases, the proteins most structurally related to beta toxin include human endonuclease HAP1, Escherichia coli endonuclease III, bovine pancreatic DNase I, and the endonuclease domain of TRAS1 from Bombyx mori. Our biological assays demonstrated for the first time that beta toxin kills proliferating human lymphocytes. Structure-directed active site mutations show that biological activities, including hemolysis and lymphotoxicity, are due to the sphingomyelinase activity of the enzyme.
-
-	Structure and biological activities of beta toxin from Staphylococcus aureus.,Huseby M, Shi K, Brown CK, Digre J, Mengistu F, Seo KS, Bohach GA, Schlievert PM, Ohlendorf DH, Earhart CA J Bacteriol. 2007 Dec;189(23):8719-26. Epub 2007 Sep 14. PMID:17873030<ref>PMID:17873030</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3i46" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Hemolysin 3D structures\|Hemolysin 3D structures]]		*[[Hemolysin 3D structures\|Hemolysin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Staphylococcus aureus ~~rn4220~~]]	+	[[Category: Staphylococcus aureus subsp. aureus RN4220]]
-	[[Category: Huseby, M]]	+	[[Category: Huseby M]]
-	[[Category: Kruse~~, A C~~]]	+	[[Category: Kruse AC]]
-	[[Category: Ohlendorf~~, D H~~]]	+	[[Category: Ohlendorf DH]]
-	[[Category: Shi, K]]	+	[[Category: Shi K]]
-	~~[[Category: Beta toxin]]~~	+
-	~~[[Category: Hemolysin]]~~	+
-	~~[[Category: Sphingomyelinase]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 12:07, 13 October 2021

OCA — Wed, 13 Oct 2021 12:07:47 GMT

←Older revision		Revision as of 12:07, 13 October 2021
Line 1:		Line 1:

	==Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound calcium ions==		==Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound calcium ions==
-	<StructureSection load='3i46' size='340' side='right' caption='[[3i46]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='3i46' size='340' side='right'caption='[[3i46]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i46]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Staphylococcus_aureus_rn4220 Staphylococcus aureus rn4220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3I46 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i46]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_rn4220 Staphylococcus aureus rn4220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I46 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i41\|3i41]], [[3i48\|3i48]], [[3i5v\|3i5v]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3i41\|3i41]], [[3i48\|3i48]], [[3i5v\|3i5v]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hlb ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=561307 Staphylococcus aureus RN4220])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hlb ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=561307 Staphylococcus aureus RN4220])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3i46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i46 OCA], [~~http~~://pdbe.org/3i46 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3i46 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3i46 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3i46 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i46 OCA], [https://pdbe.org/3i46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i46 RCSB], [https://www.ebi.ac.uk/pdbsum/3i46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i46 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Hemolysin\|Hemolysin]]	+	*[[Hemolysin 3D structures\|Hemolysin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Staphylococcus aureus rn4220]]		[[Category: Staphylococcus aureus rn4220]]
	[[Category: Huseby, M]]		[[Category: Huseby, M]]

OCA at 09:35, 19 December 2018

OCA — Wed, 19 Dec 2018 09:35:16 GMT

←Older revision		Revision as of 09:35, 19 December 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i4/3i46_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i4/3i46_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:30, 9 December 2016

OCA — Fri, 09 Dec 2016 20:30:21 GMT

←Older revision		Revision as of 20:30, 9 December 2016
Line 1:		Line 1:
		+
	==Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound calcium ions==		==Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound calcium ions==
	<StructureSection load='3i46' size='340' side='right' caption='[[3i46]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3i46' size='340' side='right' caption='[[3i46]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i46]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus~~ Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I46 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i46]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_rn4220 Staphylococcus aureus rn4220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I46 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i41\|3i41]], [[3i48\|3i48]], [[3i5v\|3i5v]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i41\|3i41]], [[3i48\|3i48]], [[3i5v\|3i5v]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hlb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1280~~ Staphylococcus aureus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hlb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=561307 Staphylococcus aureus RN4220])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i46 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i46 RCSB], [http://www.ebi.ac.uk/pdbsum/3i46 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i46 OCA], [http://pdbe.org/3i46 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i46 RCSB], [http://www.ebi.ac.uk/pdbsum/3i46 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3i46 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i46 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3i46" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Staphylococcus aureus]]	+	[[Category: Staphylococcus aureus rn4220]]
	[[Category: Huseby, M]]		[[Category: Huseby, M]]
	[[Category: Kruse, A C]]		[[Category: Kruse, A C]]

OCA at 09:02, 8 December 2014

OCA — Mon, 08 Dec 2014 09:02:02 GMT

←Older revision		Revision as of 09:02, 8 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3i46.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound calcium ions==
		+	<StructureSection load='3i46' size='340' side='right' caption='[[3i46]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3i46]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I46 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i41\|3i41]], [[3i48\|3i48]], [[3i5v\|3i5v]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hlb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i46 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i46 RCSB], [http://www.ebi.ac.uk/pdbsum/3i46 PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i4/3i46_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Beta toxin is a neutral sphingomyelinase secreted by certain strains of Staphylococcus aureus. This virulence factor lyses erythrocytes in order to evade the host immune system as well as scavenge nutrients. The structure of beta toxin was determined at 2.4-A resolution using crystals that were merohedrally twinned. This structure is similar to that of the sphingomyelinases of Listeria ivanovii and Bacillus cereus. Beta toxin belongs to the DNase I folding superfamily; in addition to sphingomyelinases, the proteins most structurally related to beta toxin include human endonuclease HAP1, Escherichia coli endonuclease III, bovine pancreatic DNase I, and the endonuclease domain of TRAS1 from Bombyx mori. Our biological assays demonstrated for the first time that beta toxin kills proliferating human lymphocytes. Structure-directed active site mutations show that biological activities, including hemolysis and lymphotoxicity, are due to the sphingomyelinase activity of the enzyme.

-	~~{{STRUCTURE_3i46\| PDB=3i46 \| SCENE= }}~~	+	Structure and biological activities of beta toxin from Staphylococcus aureus.,Huseby M, Shi K, Brown CK, Digre J, Mengistu F, Seo KS, Bohach GA, Schlievert PM, Ohlendorf DH, Earhart CA J Bacteriol. 2007 Dec;189(23):8719-26. Epub 2007 Sep 14. PMID:17873030<ref>PMID:17873030</ref>

-	~~===Crystal structure of beta toxin from Staphylococcus aureus F277A~~, ~~P278A mutant with bound calcium ions===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17873030}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3i46]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Staphylococcus_aureus Staphylococcus aureus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA].~~	+

	==See Also==		==See Also==
	*[[Hemolysin\|Hemolysin]]		*[[Hemolysin\|Hemolysin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:017873030<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Huseby, M.]]	+	[[Category: Huseby, M]]
-	[[Category: Kruse, A C.]]	+	[[Category: Kruse, A C]]
-	[[Category: Ohlendorf, D H.]]	+	[[Category: Ohlendorf, D H]]
-	[[Category: Shi, K.]]	+	[[Category: Shi, K]]
	[[Category: Beta toxin]]		[[Category: Beta toxin]]
	[[Category: Hemolysin]]		[[Category: Hemolysin]]
	[[Category: Sphingomyelinase]]		[[Category: Sphingomyelinase]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 14:29, 20 October 2012

OCA — Sat, 20 Oct 2012 14:29:54 GMT

←Older revision		Revision as of 14:29, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3i46]] is a 2 chain structure ~~of [[Hemolysin]]~~ with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA].	+	[[3i46]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA].

	==See Also==		==See Also==

OCA: Protected "3i46" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 15:20:30 GMT

Protected "3i46" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:20, 26 July 2012

OCA at 15:20, 26 July 2012

OCA — Thu, 26 Jul 2012 15:20:26 GMT

←Older revision		Revision as of 15:20, 26 July 2012
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-	~~{{Seed}}~~	+	[[Image:3i46.png\|left\|200px]]
-	[[Image:3i46.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3i46", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3i46\| PDB=3i46 \| SCENE= }}		{{STRUCTURE_3i46\| PDB=3i46 \| SCENE= }}

	===Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound calcium ions===		===Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound calcium ions===

		+	{{ABSTRACT_PUBMED_17873030}}

	==About this Structure==		==About this Structure==
-	~~3I46~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_rn4220~~ Staphylococcus aureus ~~rn4220~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA].	+	[[3i46]] is a 2 chain structure of [[Hemolysin]] with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA].
		+
		+	==See Also==
		+	*[[Hemolysin\|Hemolysin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17873030~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017873030</ref><references group="xtra"/>
-	[[Category: Staphylococcus aureus ~~rn4220~~]]	+	[[Category: Staphylococcus aureus]]
	[[Category: Huseby, M.]]		[[Category: Huseby, M.]]
	[[Category: Kruse, A C.]]		[[Category: Kruse, A C.]]
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	[[Category: Sphingomyelinase]]		[[Category: Sphingomyelinase]]
	[[Category: Toxin]]		[[Category: Toxin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 16:18:27 2010''

OCA at 12:12, 14 July 2010

OCA — Wed, 14 Jul 2010 12:12:19 GMT

←Older revision		Revision as of 12:12, 14 July 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3i46.jpg\|left\|200px]]

-	The ~~entry 3i46~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3i46", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3i46\| PDB=3i46 \| SCENE= }}

-	~~Authors: Huseby~~, ~~M., Shi, K., Kruse, A.C., Ohlendorf, D.H.~~	+	===Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound calcium ions===

-	Description: Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound calcium ions

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Aug 5 09~~:47:~~21 2009~~''	+	==About this Structure==
		+	3I46 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_rn4220 Staphylococcus aureus rn4220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I46 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:17873030</ref><references group="xtra"/>
		+	[[Category: Staphylococcus aureus rn4220]]
		+	[[Category: Huseby, M.]]
		+	[[Category: Kruse, A C.]]
		+	[[Category: Ohlendorf, D H.]]
		+	[[Category: Shi, K.]]
		+	[[Category: Beta toxin]]
		+	[[Category: Hemolysin]]
		+	[[Category: Sphingomyelinase]]
		+	[[Category: Toxin]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 16:18:27 2010''

OCA at 06:47, 5 August 2009

OCA — Wed, 05 Aug 2009 06:47:22 GMT

←Older revision		Revision as of 06:47, 5 August 2009
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3i46 is ON HOLD	+	The entry 3i46 is ON HOLD until Paper Publication

	Authors: Huseby, M., Shi, K., Kruse, A.C., Ohlendorf, D.H.		Authors: Huseby, M., Shi, K., Kruse, A.C., Ohlendorf, D.H.
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	Description: Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound calcium ions		Description: Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound calcium ions

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jul 15 08~~:29:41 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 5 09:47:21 2009''